Haemophilus ducreyi (strain 35000HP / ATCC 700724)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Haemophilus; [Haemophilus] ducreyi

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q7VMB8|Y1071_HAEDU UPF0756 membrane protein HD_1071 OS=Haemophilus ducreyi (strain 35000HP / ATCC 700724) OX=233412 GN=HD_1071 PE=3 SV=1
MM1 pKa = 7.38NPDD4 pKa = 4.27LDD6 pKa = 3.89PTTVRR11 pKa = 11.84FTDD14 pKa = 3.17MHH16 pKa = 6.59KK17 pKa = 9.97WICEE21 pKa = 3.46IDD23 pKa = 3.95EE24 pKa = 5.07FDD26 pKa = 6.26DD27 pKa = 5.82DD28 pKa = 4.2PQASNEE34 pKa = 4.2YY35 pKa = 9.21ILEE38 pKa = 4.93AILSIWLEE46 pKa = 4.31EE47 pKa = 4.2YY48 pKa = 10.46QQ49 pKa = 4.5

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7VN40|Q7VN40_HAEDU Putative xylanase A OS=Haemophilus ducreyi (strain 35000HP / ATCC 700724) OX=233412 GN=HD_0746 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1694

0

1694

474521

30

4919

280.1

31.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.6 ± 0.07

1.067 ± 0.028

4.893 ± 0.043

6.028 ± 0.062

4.32 ± 0.048

6.232 ± 0.056

2.218 ± 0.03

7.291 ± 0.051

6.181 ± 0.062

10.629 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.041

4.801 ± 0.068

3.66 ± 0.033

5.129 ± 0.055

4.556 ± 0.051

5.747 ± 0.056

5.425 ± 0.031

6.46 ± 0.057

1.102 ± 0.025

3.196 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski