Bovine parvovirus (isolate pAT153/1986) (BPV)
Average proteome isoelectric point is 7.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P07297|CAPSD_PAVBP Minor capsid protein VP1 OS=Bovine parvovirus (isolate pAT153/1986) OX=648243 PE=3 SV=1
MM1 pKa = 6.89 QPSRR5 pKa = 11.84 GLYY8 pKa = 9.71 SALHH12 pKa = 5.58 SFKK15 pKa = 10.77 HH16 pKa = 5.22 LLITIRR22 pKa = 11.84 QRR24 pKa = 11.84 THH26 pKa = 6.98 APDD29 pKa = 3.45 TVLSRR34 pKa = 11.84 DD35 pKa = 4.34 EE36 pKa = 4.08 LNAWNWLQKK45 pKa = 9.56 IAQRR49 pKa = 11.84 VMRR52 pKa = 11.84 GDD54 pKa = 3.15 MDD56 pKa = 5.98 DD57 pKa = 3.64 IVDD60 pKa = 3.5 ILKK63 pKa = 10.22 CRR65 pKa = 11.84 KK66 pKa = 10.02 ANGTLVAQAINGTEE80 pKa = 4.63 FITRR84 pKa = 11.84 YY85 pKa = 8.38 MLPKK89 pKa = 9.43 NRR91 pKa = 11.84 KK92 pKa = 8.5 VADD95 pKa = 3.77 TVLTRR100 pKa = 11.84 HH101 pKa = 5.27 TTPEE105 pKa = 3.59 QSYY108 pKa = 10.86 DD109 pKa = 3.74 STWGKK114 pKa = 8.49 TYY116 pKa = 10.84 GFAVCNGEE124 pKa = 4.31 TVSEE128 pKa = 4.47 FTRR131 pKa = 11.84 KK132 pKa = 10.1 DD133 pKa = 3.1 LWKK136 pKa = 10.37 VLYY139 pKa = 10.31 NIYY142 pKa = 8.86 TAHH145 pKa = 7.21 PAEE148 pKa = 4.63 NMLNSNPSVWGDD160 pKa = 3.46 LPRR163 pKa = 11.84 VSANRR168 pKa = 11.84 IDD170 pKa = 4.9 ADD172 pKa = 3.7 DD173 pKa = 4.16 AEE175 pKa = 4.56 ARR177 pKa = 11.84 SRR179 pKa = 11.84 PIKK182 pKa = 10.36 LSRR185 pKa = 11.84 KK186 pKa = 7.07 QKK188 pKa = 10.21 IMAEE192 pKa = 4.37 VIQRR196 pKa = 11.84 ATDD199 pKa = 3.86 GLLLTYY205 pKa = 10.75 NDD207 pKa = 4.26 LVVHH211 pKa = 6.58 LSDD214 pKa = 5.14 LMLMLEE220 pKa = 4.41 GMPGGSKK227 pKa = 8.51 TAEE230 pKa = 3.89 QLLTMIHH237 pKa = 6.5 IKK239 pKa = 10.67 LCAKK243 pKa = 9.28 YY244 pKa = 9.96 NAYY247 pKa = 10.35 EE248 pKa = 4.04 FMLMKK253 pKa = 10.49 TPATQNMNPGAPHH266 pKa = 6.78 YY267 pKa = 9.83 DD268 pKa = 3.48 CQGNLVFKK276 pKa = 10.69 LLNLQGYY283 pKa = 8.04 NPWQVGHH290 pKa = 6.95 WLVMMLSKK298 pKa = 10.08 KK299 pKa = 7.96 TGKK302 pKa = 10.21 RR303 pKa = 11.84 NSTLFYY309 pKa = 10.93 GPASTGKK316 pKa = 8.58 TNLAKK321 pKa = 10.49 AICHH325 pKa = 5.71 AVGLYY330 pKa = 10.87 GCVNHH335 pKa = 6.51 NNKK338 pKa = 9.06 QFPFNDD344 pKa = 3.97 APNKK348 pKa = 9.42 MILWWEE354 pKa = 4.1 EE355 pKa = 4.01 CIMTTDD361 pKa = 3.4 YY362 pKa = 11.6 VEE364 pKa = 4.07 AAKK367 pKa = 10.54 CVLGGTHH374 pKa = 5.44 VRR376 pKa = 11.84 VDD378 pKa = 3.75 VKK380 pKa = 11.08 HH381 pKa = 6.37 KK382 pKa = 10.55 DD383 pKa = 3.54 SRR385 pKa = 11.84 EE386 pKa = 3.94 LPQIPVLLSSNHH398 pKa = 6.23 DD399 pKa = 3.55 VYY401 pKa = 11.37 TVVGGNATFGVHH413 pKa = 6.97 AAPLKK418 pKa = 10.4 EE419 pKa = 5.63 RR420 pKa = 11.84 ITQMNFMKK428 pKa = 10.37 QLPNTFGEE436 pKa = 4.44 ITPGMISNWLSHH448 pKa = 6.66 CAHH451 pKa = 7.26 IHH453 pKa = 5.0 QEE455 pKa = 4.07 HH456 pKa = 6.83 LSLEE460 pKa = 4.18 GFAIKK465 pKa = 9.86 WDD467 pKa = 3.79 VQSVGNSFPLQTLCPGHH484 pKa = 5.34 SQNWTFSEE492 pKa = 5.01 NGVCWHH498 pKa = 7.05 CGGFIQPTPEE508 pKa = 4.33 SDD510 pKa = 3.16 TDD512 pKa = 3.75 SDD514 pKa = 4.72 GDD516 pKa = 4.16 PDD518 pKa = 4.31 PDD520 pKa = 3.69 GAVAGDD526 pKa = 4.07 SDD528 pKa = 3.77 TSANSEE534 pKa = 4.31 STVSFSSNDD543 pKa = 3.3 SGLGSVTSSAPSVPDD558 pKa = 3.12 RR559 pKa = 11.84 AEE561 pKa = 4.22 EE562 pKa = 3.96 IEE564 pKa = 4.71 EE565 pKa = 4.16 IPSEE569 pKa = 4.03 CLEE572 pKa = 4.06 WMRR575 pKa = 11.84 EE576 pKa = 3.98 EE577 pKa = 4.12 VDD579 pKa = 3.44 RR580 pKa = 11.84 LSAHH584 pKa = 7.39 DD585 pKa = 4.12 INSLAHH591 pKa = 5.56 QATGFILDD599 pKa = 5.39 PIPEE603 pKa = 4.14 EE604 pKa = 4.24 PEE606 pKa = 3.56 EE607 pKa = 4.41 GEE609 pKa = 3.77 RR610 pKa = 11.84 DD611 pKa = 3.59 LARR614 pKa = 11.84 EE615 pKa = 3.95 DD616 pKa = 4.35 AEE618 pKa = 4.58 PEE620 pKa = 3.82 ASTSHH625 pKa = 5.79 TPATKK630 pKa = 9.76 RR631 pKa = 11.84 ARR633 pKa = 11.84 VEE635 pKa = 4.17 EE636 pKa = 4.39 GEE638 pKa = 4.35 PWDD641 pKa = 3.56 GTQPITEE648 pKa = 4.47 GDD650 pKa = 3.55 WIDD653 pKa = 3.47 FEE655 pKa = 5.14 SRR657 pKa = 11.84 QKK659 pKa = 10.42 RR660 pKa = 11.84 RR661 pKa = 11.84 RR662 pKa = 11.84 LEE664 pKa = 3.87 RR665 pKa = 11.84 EE666 pKa = 3.73 EE667 pKa = 4.89 KK668 pKa = 10.7 GGEE671 pKa = 4.05 DD672 pKa = 3.4 EE673 pKa = 5.37 DD674 pKa = 4.81 MEE676 pKa = 4.51 VQEE679 pKa = 5.19 SDD681 pKa = 3.04 PSAWGEE687 pKa = 3.75 KK688 pKa = 10.23 LGIVEE693 pKa = 4.81 KK694 pKa = 10.44 PGEE697 pKa = 4.27 EE698 pKa = 4.34 PIVLYY703 pKa = 10.73 CFEE706 pKa = 4.77 TLPEE710 pKa = 4.22 SDD712 pKa = 4.77 EE713 pKa = 4.77 EE714 pKa = 5.01 GDD716 pKa = 3.54 SDD718 pKa = 5.57 KK719 pKa = 11.35 EE720 pKa = 4.43 NKK722 pKa = 7.8 THH724 pKa = 5.93 TVV726 pKa = 3.01
Molecular weight: 81.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.011
IPC2_protein 5.003
IPC_protein 4.991
Toseland 4.965
ProMoST 5.143
Dawson 5.003
Bjellqvist 5.118
Wikipedia 4.876
Rodwell 4.927
Grimsley 4.902
Solomon 5.003
Lehninger 4.965
Nozaki 5.13
DTASelect 5.283
Thurlkill 4.978
EMBOSS 4.927
Sillero 5.207
Patrickios 3.999
IPC_peptide 5.016
IPC2_peptide 5.207
IPC2.peptide.svr19 5.135
Protein with the highest isoelectric point:
>sp|P07296|NS1_PAVBP Initiator protein NS1 OS=Bovine parvovirus (isolate pAT153/1986) OX=648243 GN=NS1 PE=3 SV=1
MM1 pKa = 7.29 EE2 pKa = 5.88 RR3 pKa = 11.84 SRR5 pKa = 11.84 SPRR8 pKa = 11.84 EE9 pKa = 3.72 TGSTSSRR16 pKa = 11.84 DD17 pKa = 3.31 KK18 pKa = 11.47 SDD20 pKa = 4.18 ADD22 pKa = 2.87 WSEE25 pKa = 3.52 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 EE29 pKa = 3.79 EE30 pKa = 4.0 RR31 pKa = 11.84 TRR33 pKa = 11.84 TWKK36 pKa = 10.31 SRR38 pKa = 11.84 SPIRR42 pKa = 11.84 ARR44 pKa = 11.84 GEE46 pKa = 3.99 RR47 pKa = 11.84 SWGSWRR53 pKa = 11.84 SRR55 pKa = 11.84 EE56 pKa = 4.13 KK57 pKa = 10.8 NQSSSTASRR66 pKa = 11.84 PYY68 pKa = 10.36 QKK70 pKa = 9.82 ATRR73 pKa = 11.84 KK74 pKa = 7.55 EE75 pKa = 3.98 TATKK79 pKa = 8.43 KK80 pKa = 8.76 TKK82 pKa = 8.68 HH83 pKa = 5.3 TPFNVFSAHH92 pKa = 6.68 RR93 pKa = 11.84 ALSKK97 pKa = 10.07 TDD99 pKa = 3.37 LQFCGFYY106 pKa = 9.43 WHH108 pKa = 5.99 STRR111 pKa = 11.84 LASKK115 pKa = 8.4 GTNEE119 pKa = 4.09 IFNGLKK125 pKa = 10.17 QSFQSKK131 pKa = 10.59 AIDD134 pKa = 4.28 GKK136 pKa = 11.05 LDD138 pKa = 3.34 WEE140 pKa = 4.96 GVRR143 pKa = 11.84 EE144 pKa = 4.4 LLFEE148 pKa = 4.28 QKK150 pKa = 10.29 KK151 pKa = 10.76 CLDD154 pKa = 2.83 TWYY157 pKa = 10.68 RR158 pKa = 11.84 NMMYY162 pKa = 10.22 HH163 pKa = 6.52 FALGGDD169 pKa = 4.11 CEE171 pKa = 4.37 KK172 pKa = 11.01 CNYY175 pKa = 8.82 WDD177 pKa = 4.26 DD178 pKa = 3.88 VYY180 pKa = 11.36 KK181 pKa = 10.92 KK182 pKa = 9.99 HH183 pKa = 6.29 LANVDD188 pKa = 3.67 TYY190 pKa = 11.65 SVAEE194 pKa = 4.66 EE195 pKa = 4.08 ITDD198 pKa = 4.2 SEE200 pKa = 4.27 MLEE203 pKa = 3.92 AAEE206 pKa = 5.29 AVDD209 pKa = 5.04 AANQQ213 pKa = 3.32
Molecular weight: 24.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.77
IPC2_protein 8.58
IPC_protein 8.521
Toseland 9.253
ProMoST 9.033
Dawson 9.531
Bjellqvist 9.311
Wikipedia 9.677
Rodwell 9.794
Grimsley 9.56
Solomon 9.589
Lehninger 9.56
Nozaki 9.487
DTASelect 9.238
Thurlkill 9.399
EMBOSS 9.692
Sillero 9.531
Patrickios 5.181
IPC_peptide 9.589
IPC2_peptide 7.98
IPC2.peptide.svr19 7.772
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
2
5
2730
213
726
546.0
61.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.85 ± 0.208
1.099 ± 0.275
5.604 ± 0.274
5.275 ± 0.973
2.637 ± 0.177
7.766 ± 0.486
3.15 ± 0.128
4.835 ± 0.389
4.945 ± 0.492
6.63 ± 0.471
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.144
6.63 ± 0.611
5.238 ± 0.422
4.835 ± 0.441
5.861 ± 0.577
6.923 ± 0.532
6.74 ± 0.128
6.117 ± 0.444
2.344 ± 0.194
3.736 ± 0.461
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here