Trichoplax adhaerens (Trichoplax reptans)
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11520 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3RQ32|B3RQ32_TRIAD 2-Hacid_dh_C domain-containing protein OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_53759 PE=4 SV=1
MM1 pKa = 6.47 YY2 pKa = 9.56 TPEE5 pKa = 5.08 ASIQTEE11 pKa = 3.82 SDD13 pKa = 3.34 IIADD17 pKa = 3.24 ITSQDD22 pKa = 3.44 EE23 pKa = 4.19 NLKK26 pKa = 8.21 EE27 pKa = 4.2 TRR29 pKa = 11.84 STILLSDD36 pKa = 3.4 VDD38 pKa = 4.3 IEE40 pKa = 4.25 LSSTIGNEE48 pKa = 4.14 EE49 pKa = 3.89 EE50 pKa = 5.06 DD51 pKa = 4.27 KK52 pKa = 11.27 EE53 pKa = 4.5 STNIDD58 pKa = 3.83 NIISATDD65 pKa = 3.25 IQEE68 pKa = 4.01 SSKK71 pKa = 10.99 DD72 pKa = 3.4 QSILHH77 pKa = 6.12 TLQSTIDD84 pKa = 3.68 NFEE87 pKa = 3.88 VSSPVNNIEE96 pKa = 4.15 SSSIDD101 pKa = 3.4 IISDD105 pKa = 3.36 KK106 pKa = 11.06 VSIYY110 pKa = 10.87 SDD112 pKa = 3.57 GLLQTITQNVMYY124 pKa = 9.19 TPEE127 pKa = 4.68 ASIQTEE133 pKa = 3.82 SDD135 pKa = 3.34 IIADD139 pKa = 3.24 ITSQDD144 pKa = 3.44 EE145 pKa = 4.19 NLKK148 pKa = 8.21 EE149 pKa = 4.19 TRR151 pKa = 11.84 STILISDD158 pKa = 3.52 VDD160 pKa = 3.88 IEE162 pKa = 4.21 LSSTIGNEE170 pKa = 4.01 EE171 pKa = 4.11 EE172 pKa = 4.48 EE173 pKa = 4.5 KK174 pKa = 10.91 EE175 pKa = 4.22 STNIDD180 pKa = 3.84 NIISATDD187 pKa = 3.25 IQEE190 pKa = 4.01 SSKK193 pKa = 10.98 DD194 pKa = 3.47 QSILYY199 pKa = 7.97 TLQSTIDD206 pKa = 3.65 NFEE209 pKa = 3.88 VSSPVNNIEE218 pKa = 4.37 SNSIDD223 pKa = 3.69 IISDD227 pKa = 3.69 KK228 pKa = 10.87 ISIYY232 pKa = 10.8 SDD234 pKa = 3.16 GLSQTNTQNIMYY246 pKa = 8.7 TPEE249 pKa = 4.55 ASIQTEE255 pKa = 3.82 SDD257 pKa = 3.34 IIADD261 pKa = 3.23 ITSQDD266 pKa = 2.85 EE267 pKa = 4.33 HH268 pKa = 8.04 LQEE271 pKa = 4.45 TQSTTLISDD280 pKa = 3.51 VSIEE284 pKa = 4.09 LSNTIQSEE292 pKa = 4.55 EE293 pKa = 4.11 EE294 pKa = 3.8 KK295 pKa = 9.96 STNIDD300 pKa = 3.25 NSILTTDD307 pKa = 3.27 IQEE310 pKa = 4.28 SSEE313 pKa = 4.07 DD314 pKa = 3.58 QPILHH319 pKa = 6.77 TLQSTIDD326 pKa = 3.53 NFKK329 pKa = 10.45 ISSSVDD335 pKa = 3.36 NIEE338 pKa = 4.49 SNSIDD343 pKa = 3.59 IISDD347 pKa = 3.33 KK348 pKa = 11.06 VSIYY352 pKa = 10.87 SDD354 pKa = 3.57 GLLQTITQNIMYY366 pKa = 8.73 TPEE369 pKa = 4.55 ASIQTGSDD377 pKa = 3.36 IIADD381 pKa = 3.18 ITSQDD386 pKa = 3.27 EE387 pKa = 4.13 NLQEE391 pKa = 4.27 TRR393 pKa = 11.84 STTLISDD400 pKa = 3.43 VDD402 pKa = 3.73 IEE404 pKa = 4.21 LSSTIGNEE412 pKa = 4.01 EE413 pKa = 4.11 EE414 pKa = 4.48 EE415 pKa = 4.5 KK416 pKa = 10.91 EE417 pKa = 4.22 STNIDD422 pKa = 3.78 NIISPTGGNEE432 pKa = 3.6 IVVTKK437 pKa = 10.65 SIGFMYY443 pKa = 10.4 HH444 pKa = 6.86 SSDD447 pKa = 3.21 QSAVTDD453 pKa = 3.53 EE454 pKa = 4.42 FSSVEE459 pKa = 3.84 IFNNALSISVNHH471 pKa = 6.65 SNFQTIQEE479 pKa = 4.14 NSDD482 pKa = 3.77 FEE484 pKa = 4.4 LSNIISTNGYY494 pKa = 10.31 ASLPPIEE501 pKa = 5.85 LSTQEE506 pKa = 3.8 YY507 pKa = 10.45 ASRR510 pKa = 11.84 SNHH513 pKa = 4.61 EE514 pKa = 4.3 TYY516 pKa = 11.08 LLQASSDD523 pKa = 4.21 SINGFTMFNSLSSLVDD539 pKa = 3.65 SVSSNGNFEE548 pKa = 4.56 QIASVPEE555 pKa = 4.0 SGISSMEE562 pKa = 4.11 SVNDD566 pKa = 3.28 AVTFTNAVGPFQSIFNSRR584 pKa = 11.84 NDD586 pKa = 3.0 ISLSQWNNIAGASYY600 pKa = 11.13 AVTTSDD606 pKa = 3.76 PLIAFVGSVSSDD618 pKa = 3.27 HH619 pKa = 6.35 YY620 pKa = 11.1 SHH622 pKa = 7.59 TINHH626 pKa = 5.53 EE627 pKa = 4.24 TQSILIMASTDD638 pKa = 3.16 SSSEE642 pKa = 4.39 SNSSAPDD649 pKa = 4.23 DD650 pKa = 3.78 IYY652 pKa = 11.62 SEE654 pKa = 4.07 IRR656 pKa = 11.84 GSISSSAVDD665 pKa = 3.31 TLTISMNNYY674 pKa = 10.41 SMLSSSTYY682 pKa = 9.98 PFPLDD687 pKa = 3.28 PHH689 pKa = 5.92 EE690 pKa = 4.73 TQSSTHH696 pKa = 3.87 THH698 pKa = 5.47 YY699 pKa = 11.28 GEE701 pKa = 4.51 SYY703 pKa = 9.66 RR704 pKa = 11.84 EE705 pKa = 4.11 SPASILLDD713 pKa = 3.24 HH714 pKa = 7.09 KK715 pKa = 11.6 SNVTSFVQSSNLAQIVNVTGSDD737 pKa = 3.29 GLDD740 pKa = 3.21 NVEE743 pKa = 4.15 TSSSIVAFSPTFFSSFYY760 pKa = 10.86 SGDD763 pKa = 3.27 GNIYY767 pKa = 10.13 DD768 pKa = 3.73 QSSTNSMIDD777 pKa = 3.52 DD778 pKa = 4.91 NIAPEE783 pKa = 4.16 PSSVLEE789 pKa = 3.95 NTEE792 pKa = 4.48 TISKK796 pKa = 8.89 QQTIEE801 pKa = 4.12 DD802 pKa = 4.42 CSTNMNLCDD811 pKa = 3.72 TNAICTNTLGSFICSCVTGFTGNGTFCEE839 pKa = 4.45 GKK841 pKa = 10.04 EE842 pKa = 3.95 FQSLYY847 pKa = 11.0 LHH849 pKa = 6.82 FLL851 pKa = 3.66
Molecular weight: 92.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.478
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.567
Rodwell 3.516
Grimsley 3.376
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.516
EMBOSS 3.579
Sillero 3.808
Patrickios 0.973
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|B3RZS5|B3RZS5_TRIAD Glyco_tran_10_N domain-containing protein OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_26273 PE=3 SV=1
SS1 pKa = 6.89 HH2 pKa = 7.64 KK3 pKa = 11.01 SFIIKK8 pKa = 10.17 RR9 pKa = 11.84 KK10 pKa = 8.59 LAKK13 pKa = 10.03 KK14 pKa = 8.72 MKK16 pKa = 9.55 QNRR19 pKa = 11.84 PVPQWIRR26 pKa = 11.84 LRR28 pKa = 11.84 TNNKK32 pKa = 8.55 IRR34 pKa = 11.84 YY35 pKa = 5.25 NTKK38 pKa = 8.17 RR39 pKa = 11.84 RR40 pKa = 11.84 HH41 pKa = 3.92 WRR43 pKa = 11.84 RR44 pKa = 11.84 TKK46 pKa = 10.83 LGLL49 pKa = 3.67
Molecular weight: 6.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11518
2
11520
5222991
49
7710
453.4
51.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.994 ± 0.021
2.096 ± 0.027
5.688 ± 0.016
5.807 ± 0.029
4.103 ± 0.017
5.071 ± 0.028
2.342 ± 0.011
7.327 ± 0.021
6.7 ± 0.028
9.376 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.171 ± 0.009
5.844 ± 0.019
3.87 ± 0.021
4.204 ± 0.017
4.884 ± 0.017
8.249 ± 0.026
5.573 ± 0.02
5.725 ± 0.015
1.122 ± 0.007
3.855 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here