Clostridium sp. CAG:411

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2623 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7HUT0|R7HUT0_9CLOT DNA internalization-related competence protein ComEC/Rec2 OS=Clostridium sp. CAG:411 OX=1262802 GN=BN648_01560 PE=4 SV=1
MM1 pKa = 7.34SLDD4 pKa = 3.33ISSLSGTSNLNSIYY18 pKa = 10.63NNNYY22 pKa = 7.81STDD25 pKa = 3.53RR26 pKa = 11.84VSNFSNSYY34 pKa = 10.48DD35 pKa = 3.56GLISSVKK42 pKa = 10.69DD43 pKa = 3.23EE44 pKa = 4.52GDD46 pKa = 3.26SFEE49 pKa = 5.66NVFQSALDD57 pKa = 4.63LINTTNDD64 pKa = 3.21YY65 pKa = 10.95TNVAEE70 pKa = 4.67QEE72 pKa = 4.21EE73 pKa = 4.87LNYY76 pKa = 11.32AMGLSEE82 pKa = 4.6DD83 pKa = 3.66THH85 pKa = 8.0TLMIAQQKK93 pKa = 10.56ANISLAYY100 pKa = 8.45TVAVRR105 pKa = 11.84DD106 pKa = 4.08AVVDD110 pKa = 4.44AYY112 pKa = 11.64NNIMNMQFF120 pKa = 2.96

Molecular weight: 13.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7I4G8|R7I4G8_9CLOT Hydrolyase tartrate beta subunit/fumarate domain protein Fe-S type OS=Clostridium sp. CAG:411 OX=1262802 GN=BN648_00011 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.1SGRR28 pKa = 11.84KK29 pKa = 8.52VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2623

0

2623

861530

31

2486

328.5

37.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.79 ± 0.047

1.465 ± 0.02

5.346 ± 0.039

7.888 ± 0.053

3.994 ± 0.031

6.516 ± 0.042

1.564 ± 0.021

7.571 ± 0.044

8.374 ± 0.045

8.533 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.974 ± 0.026

4.783 ± 0.041

2.825 ± 0.032

3.662 ± 0.031

3.639 ± 0.037

5.867 ± 0.046

5.875 ± 0.062

7.102 ± 0.043

0.854 ± 0.017

4.372 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski