Amino acid dipepetide frequency for Clostridium sp. CAG:411

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.024AlaAla: 5.024 ± 0.102	0.974AlaCys: 0.974 ± 0.035	3.743AlaAsp: 3.743 ± 0.071	4.652AlaGlu: 4.652 ± 0.084	2.879AlaPhe: 2.879 ± 0.072	4.938AlaGly: 4.938 ± 0.082	0.951AlaHis: 0.951 ± 0.033	5.258AlaIle: 5.258 ± 0.086	5.696AlaLys: 5.696 ± 0.085	6.042AlaLeu: 6.042 ± 0.091	2.153AlaMet: 2.153 ± 0.056	2.898AlaAsn: 2.898 ± 0.068	1.682AlaPro: 1.682 ± 0.057	2.158AlaGln: 2.158 ± 0.052	2.309AlaArg: 2.309 ± 0.063	3.905AlaSer: 3.905 ± 0.084	4.204AlaThr: 4.204 ± 0.109	5.128AlaVal: 5.128 ± 0.077	0.518AlaTrp: 0.518 ± 0.025	2.781AlaTyr: 2.781 ± 0.052	0.007AlaXaa: 0.007 ± 0.003
Cys	0.923CysAla: 0.923 ± 0.034	0.296CysCys: 0.296 ± 0.02	0.765CysAsp: 0.765 ± 0.026	0.936CysGlu: 0.936 ± 0.034	0.677CysPhe: 0.677 ± 0.029	1.218CysGly: 1.218 ± 0.045	0.296CysHis: 0.296 ± 0.021	1.257CysIle: 1.257 ± 0.037	1.04CysLys: 1.04 ± 0.035	1.117CysLeu: 1.117 ± 0.04	0.46CysMet: 0.46 ± 0.023	0.683CysAsn: 0.683 ± 0.036	0.497CysPro: 0.497 ± 0.029	0.476CysGln: 0.476 ± 0.025	0.51CysArg: 0.51 ± 0.023	0.919CysSer: 0.919 ± 0.035	0.741CysThr: 0.741 ± 0.028	1.07CysVal: 1.07 ± 0.034	0.115CysTrp: 0.115 ± 0.011	0.659CysTyr: 0.659 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	3.943AspAla: 3.943 ± 0.077	0.832AspCys: 0.832 ± 0.036	2.543AspAsp: 2.543 ± 0.069	4.312AspGlu: 4.312 ± 0.074	2.505AspPhe: 2.505 ± 0.063	4.219AspGly: 4.219 ± 0.099	0.656AspHis: 0.656 ± 0.029	4.325AspIle: 4.325 ± 0.078	4.037AspLys: 4.037 ± 0.072	4.246AspLeu: 4.246 ± 0.085	1.714AspMet: 1.714 ± 0.049	2.23AspAsn: 2.23 ± 0.058	1.2AspPro: 1.2 ± 0.04	1.278AspGln: 1.278 ± 0.039	1.702AspArg: 1.702 ± 0.051	3.122AspSer: 3.122 ± 0.068	3.453AspThr: 3.453 ± 0.068	3.948AspVal: 3.948 ± 0.069	0.517AspTrp: 0.517 ± 0.027	2.672AspTyr: 2.672 ± 0.054	0.002AspXaa: 0.002 ± 0.001
Glu	4.959GluAla: 4.959 ± 0.095	0.822GluCys: 0.822 ± 0.035	4.347GluAsp: 4.347 ± 0.081	7.973GluGlu: 7.973 ± 0.17	2.496GluPhe: 2.496 ± 0.059	4.145GluGly: 4.145 ± 0.078	1.286GluHis: 1.286 ± 0.041	5.822GluIle: 5.822 ± 0.092	8.675GluLys: 8.675 ± 0.133	6.422GluLeu: 6.422 ± 0.094	2.462GluMet: 2.462 ± 0.052	4.7GluAsn: 4.7 ± 0.075	1.769GluPro: 1.769 ± 0.057	4.177GluGln: 4.177 ± 0.085	3.257GluArg: 3.257 ± 0.076	3.041GluSer: 3.041 ± 0.058	3.926GluThr: 3.926 ± 0.079	4.687GluVal: 4.687 ± 0.077	0.681GluTrp: 0.681 ± 0.028	3.231GluTyr: 3.231 ± 0.072	0.0GluXaa: 0.0 ± 0.0
Phe	2.771PheAla: 2.771 ± 0.066	0.713PheCys: 0.713 ± 0.031	2.314PheAsp: 2.314 ± 0.05	2.643PheGlu: 2.643 ± 0.063	1.878PhePhe: 1.878 ± 0.054	2.772PheGly: 2.772 ± 0.072	0.745PheHis: 0.745 ± 0.029	2.675PheIle: 2.675 ± 0.062	2.136PheLys: 2.136 ± 0.047	3.861PheLeu: 3.861 ± 0.099	1.107PheMet: 1.107 ± 0.035	1.505PheAsn: 1.505 ± 0.044	1.17PhePro: 1.17 ± 0.038	1.523PheGln: 1.523 ± 0.054	1.446PheArg: 1.446 ± 0.044	3.159PheSer: 3.159 ± 0.059	2.339PheThr: 2.339 ± 0.057	2.931PheVal: 2.931 ± 0.069	0.409PheTrp: 0.409 ± 0.025	1.844PheTyr: 1.844 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	4.18GlyAla: 4.18 ± 0.094	1.173GlyCys: 1.173 ± 0.047	3.178GlyAsp: 3.178 ± 0.066	4.644GlyGlu: 4.644 ± 0.073	2.658GlyPhe: 2.658 ± 0.057	3.949GlyGly: 3.949 ± 0.094	1.008GlyHis: 1.008 ± 0.038	5.9GlyIle: 5.9 ± 0.103	6.334GlyLys: 6.334 ± 0.085	4.86GlyLeu: 4.86 ± 0.079	2.251GlyMet: 2.251 ± 0.056	3.337GlyAsn: 3.337 ± 0.061	1.018GlyPro: 1.018 ± 0.033	1.912GlyGln: 1.912 ± 0.048	2.237GlyArg: 2.237 ± 0.051	3.711GlySer: 3.711 ± 0.079	4.296GlyThr: 4.296 ± 0.092	4.769GlyVal: 4.769 ± 0.082	0.573GlyTrp: 0.573 ± 0.025	3.165GlyTyr: 3.165 ± 0.074	0.008GlyXaa: 0.008 ± 0.003
His	1.011HisAla: 1.011 ± 0.033	0.269HisCys: 0.269 ± 0.017	0.738HisAsp: 0.738 ± 0.029	0.889HisGlu: 0.889 ± 0.029	0.822HisPhe: 0.822 ± 0.029	1.069HisGly: 1.069 ± 0.035	0.325HisHis: 0.325 ± 0.026	1.371HisIle: 1.371 ± 0.041	1.069HisLys: 1.069 ± 0.035	1.326HisLeu: 1.326 ± 0.039	0.526HisMet: 0.526 ± 0.026	0.775HisAsn: 0.775 ± 0.03	0.644HisPro: 0.644 ± 0.035	0.474HisGln: 0.474 ± 0.022	0.611HisArg: 0.611 ± 0.026	0.913HisSer: 0.913 ± 0.031	0.944HisThr: 0.944 ± 0.033	1.027HisVal: 1.027 ± 0.037	0.135HisTrp: 0.135 ± 0.013	0.699HisTyr: 0.699 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.721IleAla: 5.721 ± 0.08	1.342IleCys: 1.342 ± 0.045	3.986IleAsp: 3.986 ± 0.07	5.388IleGlu: 5.388 ± 0.099	2.919IlePhe: 2.919 ± 0.075	5.122IleGly: 5.122 ± 0.099	1.232IleHis: 1.232 ± 0.037	5.173IleIle: 5.173 ± 0.098	5.271IleLys: 5.271 ± 0.077	7.047IleLeu: 7.047 ± 0.108	1.913IleMet: 1.913 ± 0.05	3.156IleAsn: 3.156 ± 0.059	2.833IlePro: 2.833 ± 0.062	2.533IleGln: 2.533 ± 0.052	2.963IleArg: 2.963 ± 0.058	5.157IleSer: 5.157 ± 0.094	4.752IleThr: 4.752 ± 0.089	5.598IleVal: 5.598 ± 0.096	0.611IleTrp: 0.611 ± 0.029	2.989IleTyr: 2.989 ± 0.067	0.001IleXaa: 0.001 ± 0.001
Lys	5.36LysAla: 5.36 ± 0.093	0.817LysCys: 0.817 ± 0.033	4.578LysAsp: 4.578 ± 0.08	8.638LysGlu: 8.638 ± 0.132	2.162LysPhe: 2.162 ± 0.05	4.853LysGly: 4.853 ± 0.075	1.185LysHis: 1.185 ± 0.036	6.082LysIle: 6.082 ± 0.075	9.393LysLys: 9.393 ± 0.131	6.075LysLeu: 6.075 ± 0.09	2.594LysMet: 2.594 ± 0.055	4.891LysAsn: 4.891 ± 0.082	1.929LysPro: 1.929 ± 0.051	3.653LysGln: 3.653 ± 0.079	3.358LysArg: 3.358 ± 0.066	3.912LysSer: 3.912 ± 0.068	4.434LysThr: 4.434 ± 0.074	5.549LysVal: 5.549 ± 0.102	0.761LysTrp: 0.761 ± 0.033	3.515LysTyr: 3.515 ± 0.08	0.0LysXaa: 0.0 ± 0.0
Leu	5.833LeuAla: 5.833 ± 0.081	1.379LeuCys: 1.379 ± 0.037	4.659LeuAsp: 4.659 ± 0.077	6.654LeuGlu: 6.654 ± 0.12	3.751LeuPhe: 3.751 ± 0.089	5.354LeuGly: 5.354 ± 0.097	1.612LeuHis: 1.612 ± 0.048	5.302LeuIle: 5.302 ± 0.094	6.544LeuLys: 6.544 ± 0.095	8.579LeuLeu: 8.579 ± 0.143	2.245LeuMet: 2.245 ± 0.05	3.692LeuAsn: 3.692 ± 0.072	3.076LeuPro: 3.076 ± 0.059	3.583LeuGln: 3.583 ± 0.081	3.113LeuArg: 3.113 ± 0.077	5.906LeuSer: 5.906 ± 0.088	4.645LeuThr: 4.645 ± 0.09	5.373LeuVal: 5.373 ± 0.085	0.636LeuTrp: 0.636 ± 0.032	3.39LeuTyr: 3.39 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	2.005MetAla: 2.005 ± 0.055	0.359MetCys: 0.359 ± 0.02	1.851MetAsp: 1.851 ± 0.047	2.633MetGlu: 2.633 ± 0.065	1.049MetPhe: 1.049 ± 0.037	1.862MetGly: 1.862 ± 0.052	0.508MetHis: 0.508 ± 0.025	1.927MetIle: 1.927 ± 0.051	2.628MetLys: 2.628 ± 0.052	2.85MetLeu: 2.85 ± 0.074	0.788MetMet: 0.788 ± 0.034	1.435MetAsn: 1.435 ± 0.046	0.947MetPro: 0.947 ± 0.031	1.28MetGln: 1.28 ± 0.039	1.124MetArg: 1.124 ± 0.036	1.698MetSer: 1.698 ± 0.042	1.521MetThr: 1.521 ± 0.039	2.002MetVal: 2.002 ± 0.055	0.215MetTrp: 0.215 ± 0.014	1.057MetTyr: 1.057 ± 0.038	0.001MetXaa: 0.001 ± 0.001
Asn	3.438AsnAla: 3.438 ± 0.064	0.779AsnCys: 0.779 ± 0.036	2.146AsnAsp: 2.146 ± 0.051	3.1AsnGlu: 3.1 ± 0.071	1.778AsnPhe: 1.778 ± 0.046	3.767AsnGly: 3.767 ± 0.079	0.789AsnHis: 0.789 ± 0.034	3.762AsnIle: 3.762 ± 0.067	3.666AsnLys: 3.666 ± 0.07	3.811AsnLeu: 3.811 ± 0.073	1.476AsnMet: 1.476 ± 0.041	2.202AsnAsn: 2.202 ± 0.066	1.769AsnPro: 1.769 ± 0.043	1.796AsnGln: 1.796 ± 0.051	1.938AsnArg: 1.938 ± 0.06	2.652AsnSer: 2.652 ± 0.063	2.908AsnThr: 2.908 ± 0.069	3.461AsnVal: 3.461 ± 0.07	0.454AsnTrp: 0.454 ± 0.026	2.138AsnTyr: 2.138 ± 0.056	0.0AsnXaa: 0.0 ± 0.0
Pro	1.806ProAla: 1.806 ± 0.05	0.368ProCys: 0.368 ± 0.022	1.692ProAsp: 1.692 ± 0.051	2.664ProGlu: 2.664 ± 0.062	1.327ProPhe: 1.327 ± 0.039	1.717ProGly: 1.717 ± 0.048	0.399ProHis: 0.399 ± 0.025	2.224ProIle: 2.224 ± 0.048	2.107ProLys: 2.107 ± 0.055	2.138ProLeu: 2.138 ± 0.052	0.764ProMet: 0.764 ± 0.026	1.278ProAsn: 1.278 ± 0.036	0.544ProPro: 0.544 ± 0.03	0.931ProGln: 0.931 ± 0.032	0.76ProArg: 0.76 ± 0.03	1.717ProSer: 1.717 ± 0.05	1.991ProThr: 1.991 ± 0.083	2.279ProVal: 2.279 ± 0.057	0.252ProTrp: 0.252 ± 0.02	1.291ProTyr: 1.291 ± 0.039	0.003ProXaa: 0.003 ± 0.002
Gln	2.317GlnAla: 2.317 ± 0.057	0.446GlnCys: 0.446 ± 0.022	1.746GlnAsp: 1.746 ± 0.051	3.26GlnGlu: 3.26 ± 0.094	1.381GlnPhe: 1.381 ± 0.044	2.002GlnGly: 2.002 ± 0.052	0.483GlnHis: 0.483 ± 0.026	2.938GlnIle: 2.938 ± 0.062	3.533GlnLys: 3.533 ± 0.072	3.359GlnLeu: 3.359 ± 0.063	1.211GlnMet: 1.211 ± 0.039	1.832GlnAsn: 1.832 ± 0.051	0.879GlnPro: 0.879 ± 0.031	1.623GlnGln: 1.623 ± 0.054	1.31GlnArg: 1.31 ± 0.04	1.783GlnSer: 1.783 ± 0.051	1.851GlnThr: 1.851 ± 0.052	2.642GlnVal: 2.642 ± 0.064	0.319GlnTrp: 0.319 ± 0.02	1.709GlnTyr: 1.709 ± 0.045	0.001GlnXaa: 0.001 ± 0.001
Arg	2.096ArgAla: 2.096 ± 0.052	0.475ArgCys: 0.475 ± 0.022	1.652ArgAsp: 1.652 ± 0.049	3.279ArgGlu: 3.279 ± 0.076	1.507ArgPhe: 1.507 ± 0.043	1.924ArgGly: 1.924 ± 0.052	0.519ArgHis: 0.519 ± 0.023	3.043ArgIle: 3.043 ± 0.066	3.473ArgLys: 3.473 ± 0.067	3.011ArgLeu: 3.011 ± 0.07	1.404ArgMet: 1.404 ± 0.038	1.967ArgAsn: 1.967 ± 0.05	0.941ArgPro: 0.941 ± 0.031	1.4ArgGln: 1.4 ± 0.044	1.613ArgArg: 1.613 ± 0.049	1.788ArgSer: 1.788 ± 0.042	1.977ArgThr: 1.977 ± 0.05	2.403ArgVal: 2.403 ± 0.056	0.322ArgTrp: 0.322 ± 0.019	1.593ArgTyr: 1.593 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	3.875SerAla: 3.875 ± 0.087	0.852SerCys: 0.852 ± 0.034	3.244SerAsp: 3.244 ± 0.066	3.937SerGlu: 3.937 ± 0.079	2.613SerPhe: 2.613 ± 0.054	4.299SerGly: 4.299 ± 0.083	0.86SerHis: 0.86 ± 0.03	4.772SerIle: 4.772 ± 0.079	4.434SerLys: 4.434 ± 0.078	4.877SerLeu: 4.877 ± 0.086	1.898SerMet: 1.898 ± 0.047	2.899SerAsn: 2.899 ± 0.075	1.447SerPro: 1.447 ± 0.047	1.877SerGln: 1.877 ± 0.056	1.987SerArg: 1.987 ± 0.051	3.792SerSer: 3.792 ± 0.104	3.15SerThr: 3.15 ± 0.091	4.5SerVal: 4.5 ± 0.085	0.51SerTrp: 0.51 ± 0.027	2.84SerTyr: 2.84 ± 0.066	0.006SerXaa: 0.006 ± 0.003
Thr	4.353ThrAla: 4.353 ± 0.097	0.705ThrCys: 0.705 ± 0.031	3.143ThrAsp: 3.143 ± 0.07	4.215ThrGlu: 4.215 ± 0.071	2.287ThrPhe: 2.287 ± 0.059	4.107ThrGly: 4.107 ± 0.091	0.866ThrHis: 0.866 ± 0.035	4.918ThrIle: 4.918 ± 0.083	4.339ThrLys: 4.339 ± 0.087	4.873ThrLeu: 4.873 ± 0.082	1.428ThrMet: 1.428 ± 0.045	2.616ThrAsn: 2.616 ± 0.064	2.382ThrPro: 2.382 ± 0.09	1.775ThrGln: 1.775 ± 0.051	1.714ThrArg: 1.714 ± 0.049	3.388ThrSer: 3.388 ± 0.09	3.698ThrThr: 3.698 ± 0.119	4.946ThrVal: 4.946 ± 0.119	0.479ThrTrp: 0.479 ± 0.026	2.514ThrTyr: 2.514 ± 0.077	0.001ThrXaa: 0.001 ± 0.001
Val	5.04ValAla: 5.04 ± 0.083	1.219ValCys: 1.219 ± 0.037	3.971ValAsp: 3.971 ± 0.071	5.152ValGlu: 5.152 ± 0.09	2.925ValPhe: 2.925 ± 0.062	4.357ValGly: 4.357 ± 0.084	0.938ValHis: 0.938 ± 0.032	5.115ValIle: 5.115 ± 0.081	5.347ValLys: 5.347 ± 0.085	6.452ValLeu: 6.452 ± 0.085	1.841ValMet: 1.841 ± 0.048	3.047ValAsn: 3.047 ± 0.067	2.135ValPro: 2.135 ± 0.05	2.167ValGln: 2.167 ± 0.044	2.405ValArg: 2.405 ± 0.055	4.991ValSer: 4.991 ± 0.082	4.88ValThr: 4.88 ± 0.12	5.428ValVal: 5.428 ± 0.093	0.6ValTrp: 0.6 ± 0.026	3.011ValTyr: 3.011 ± 0.071	0.001ValXaa: 0.001 ± 0.001
Trp	0.4TrpAla: 0.4 ± 0.021	0.136TrpCys: 0.136 ± 0.014	0.484TrpAsp: 0.484 ± 0.026	0.608TrpGlu: 0.608 ± 0.027	0.376TrpPhe: 0.376 ± 0.02	0.539TrpGly: 0.539 ± 0.026	0.133TrpHis: 0.133 ± 0.013	0.72TrpIle: 0.72 ± 0.029	0.891TrpLys: 0.891 ± 0.034	0.739TrpLeu: 0.739 ± 0.031	0.346TrpMet: 0.346 ± 0.021	0.576TrpAsn: 0.576 ± 0.026	0.167TrpPro: 0.167 ± 0.016	0.274TrpGln: 0.274 ± 0.019	0.293TrpArg: 0.293 ± 0.018	0.511TrpSer: 0.511 ± 0.024	0.409TrpThr: 0.409 ± 0.027	0.449TrpVal: 0.449 ± 0.019	0.087TrpTrp: 0.087 ± 0.01	0.404TrpTyr: 0.404 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.841TyrAla: 2.841 ± 0.064	0.698TyrCys: 0.698 ± 0.027	2.674TyrAsp: 2.674 ± 0.069	3.298TyrGlu: 3.298 ± 0.073	1.951TyrPhe: 1.951 ± 0.054	2.946TyrGly: 2.946 ± 0.057	0.843TyrHis: 0.843 ± 0.032	3.121TyrIle: 3.121 ± 0.063	3.128TyrLys: 3.128 ± 0.06	3.642TyrLeu: 3.642 ± 0.077	1.157TyrMet: 1.157 ± 0.037	2.109TyrAsn: 2.109 ± 0.057	1.221TyrPro: 1.221 ± 0.038	1.734TyrGln: 1.734 ± 0.051	1.734TyrArg: 1.734 ± 0.042	2.601TyrSer: 2.601 ± 0.063	2.626TyrThr: 2.626 ± 0.075	2.831TyrVal: 2.831 ± 0.063	0.349TyrTrp: 0.349 ± 0.023	2.214TyrTyr: 2.214 ± 0.065	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.002	0.002XaaHis: 0.002 ± 0.002	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.002	0.002XaaArg: 0.002 ± 0.002	0.003XaaSer: 0.003 ± 0.002	0.003XaaThr: 0.003 ± 0.002	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.038XaaXaa: 0.038 ± 0.01

Statistics based on 2623 proteins (861530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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