Circovirus-like genome DCCV-13

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHD3|A0A190WHD3_9CIRC Replication-associated protein OS=Circovirus-like genome DCCV-13 OX=1788441 PE=4 SV=1
MM1 pKa = 7.26EE2 pKa = 4.86WEE4 pKa = 4.93LPNEE8 pKa = 4.45LDD10 pKa = 3.8TGCLPSPTKK19 pKa = 10.33IGPQRR24 pKa = 11.84SCSHH28 pKa = 6.68PACRR32 pKa = 11.84TYY34 pKa = 10.52EE35 pKa = 3.99VKK37 pKa = 10.29PRR39 pKa = 11.84SVQQGSNTGSCLRR52 pKa = 11.84SIPSRR57 pKa = 11.84SDD59 pKa = 3.34SLPLSPALSDD69 pKa = 2.99RR70 pKa = 11.84HH71 pKa = 5.64TANQPDD77 pKa = 4.34QKK79 pKa = 11.01PPMTMFGRR87 pKa = 11.84KK88 pKa = 5.78TPRR91 pKa = 11.84WTEE94 pKa = 3.57QDD96 pKa = 3.87LKK98 pKa = 10.49WVNDD102 pKa = 3.55LTEE105 pKa = 4.55EE106 pKa = 4.09MSKK109 pKa = 8.59PTGSEE114 pKa = 3.72YY115 pKa = 11.16GNPLLQGTFCPSKK128 pKa = 10.41QVFVFNITAHH138 pKa = 5.33SVQYY142 pKa = 11.6ALTTLNQLLLNVEE155 pKa = 4.64SLSFMDD161 pKa = 5.0LQVLEE166 pKa = 4.26NRR168 pKa = 11.84EE169 pKa = 4.15EE170 pKa = 4.19PGKK173 pKa = 10.91KK174 pKa = 9.45PVGLLILKK182 pKa = 9.73IRR184 pKa = 11.84EE185 pKa = 4.28ANFGMDD191 pKa = 3.3TQIRR195 pKa = 11.84RR196 pKa = 11.84MLYY199 pKa = 10.04LMNFEE204 pKa = 4.79EE205 pKa = 4.71EE206 pKa = 4.71SIFPIYY212 pKa = 10.26CDD214 pKa = 3.57GLIATQYY221 pKa = 9.56SLKK224 pKa = 10.59SRR226 pKa = 11.84EE227 pKa = 4.18LQRR230 pKa = 11.84VWW232 pKa = 3.02

Molecular weight: 26.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHE0|A0A190WHE0_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-13 OX=1788441 PE=4 SV=1
MM1 pKa = 7.17AVKK4 pKa = 10.37RR5 pKa = 11.84KK6 pKa = 8.59MPQSQGKK13 pKa = 7.72YY14 pKa = 6.58VKK16 pKa = 9.77KK17 pKa = 10.56RR18 pKa = 11.84KK19 pKa = 6.92STKK22 pKa = 9.31VNKK25 pKa = 10.11LQVKK29 pKa = 10.09KK30 pKa = 10.09MILGLSEE37 pKa = 4.01TKK39 pKa = 9.89HH40 pKa = 4.35YY41 pKa = 10.69HH42 pKa = 4.26STYY45 pKa = 9.36VAEE48 pKa = 4.64IKK50 pKa = 9.81TGFQYY55 pKa = 10.99NINPLYY61 pKa = 9.68WIPIGSNEE69 pKa = 3.95TSRR72 pKa = 11.84LGDD75 pKa = 3.61KK76 pKa = 10.6IFLEE80 pKa = 4.84SIDD83 pKa = 4.22MNCKK87 pKa = 9.03IDD89 pKa = 3.7RR90 pKa = 11.84SNVDD94 pKa = 2.74VGGLGRR100 pKa = 11.84FKK102 pKa = 10.98NSSIPFNLKK111 pKa = 10.47LIRR114 pKa = 11.84CTSKK118 pKa = 10.85AKK120 pKa = 10.52QGTTGDD126 pKa = 3.34AGFTLLGLPDD136 pKa = 3.57IRR138 pKa = 11.84LDD140 pKa = 3.87ALGNFANPHH149 pKa = 5.73NNLQQFQVVWDD160 pKa = 3.72YY161 pKa = 11.68RR162 pKa = 11.84GTLEE166 pKa = 5.3QMPVLNAGNDD176 pKa = 3.5QYY178 pKa = 11.82NSVMIKK184 pKa = 10.14KK185 pKa = 9.92RR186 pKa = 11.84IPINRR191 pKa = 11.84QVLYY195 pKa = 10.66DD196 pKa = 3.58GVEE199 pKa = 4.0ITGSSGFLKK208 pKa = 10.36DD209 pKa = 3.47YY210 pKa = 10.86QYY212 pKa = 11.81YY213 pKa = 10.07FYY215 pKa = 10.86FAVDD219 pKa = 3.56SNTTAGLSAVFVHH232 pKa = 7.18SDD234 pKa = 2.6ILVNFKK240 pKa = 11.31DD241 pKa = 3.57MM242 pKa = 4.41

Molecular weight: 27.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

742

232

268

247.3

28.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.987 ± 1.105

1.348 ± 0.475

5.391 ± 0.593

5.526 ± 1.261

3.908 ± 0.434

6.334 ± 0.648

2.022 ± 0.427

5.93 ± 0.793

6.199 ± 1.193

8.491 ± 1.415

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.582

4.447 ± 1.466

5.526 ± 1.133

4.447 ± 0.832

6.604 ± 1.294

7.278 ± 0.724

6.469 ± 0.456

6.065 ± 0.678

2.426 ± 0.934

4.178 ± 0.611
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski