Afipia broomeae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae;

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M8ZKW5|A0A2M8ZKW5_9BRAD Glutamate/tyrosine decarboxylase-like PLP-dependent enzyme OS=Afipia broomeae OX=56946 GN=BKA77_2963 PE=3 SV=1
MM1 pKa = 7.81GIFDD5 pKa = 4.95ALNTAVGGLQSQSFALQNISGNIANASTVGYY36 pKa = 9.86KK37 pKa = 10.31GVNTTFEE44 pKa = 4.57DD45 pKa = 4.75LIADD49 pKa = 4.22ASSPSGQVAGGVAAYY64 pKa = 9.12AQQTITTAGTVSASTVATNMAINGDD89 pKa = 4.06GFFSVQNATGTVDD102 pKa = 3.58GQPVFSGVTDD112 pKa = 3.31YY113 pKa = 11.22TRR115 pKa = 11.84AGDD118 pKa = 3.8FQVNANGYY126 pKa = 8.24LVNGAGYY133 pKa = 9.66YY134 pKa = 10.88LMGVTVDD141 pKa = 4.09PKK143 pKa = 10.31TGNPLGSVPQVLQFQNNFVPAQATSALQYY172 pKa = 10.7AANLPTKK179 pKa = 9.78PATTASTTAPAGSVTAAGGLSPGDD203 pKa = 3.58FGQNPMILGTPATPFGNSSVTGSPAASTAGPISTGTLLSSKK244 pKa = 10.63AVGSTSGTAVDD255 pKa = 4.79DD256 pKa = 3.99QTTSPVPITGTTLLAGGPGTASLTNAFANGDD287 pKa = 4.16TITVGTKK294 pKa = 9.75TITFSTTAATSTDD307 pKa = 3.33ANGGVINLGTGTVNDD322 pKa = 3.51VLKK325 pKa = 11.03AIDD328 pKa = 4.69EE329 pKa = 4.31ISTTGTASTVSGGKK343 pKa = 8.0ITLNDD348 pKa = 3.39SAGDD352 pKa = 3.52ITVTSSNTAAFTALGFSGGTVTTTAGTATGTLVNNQTTTPVPVSGTTLLSGVAGAYY408 pKa = 9.99SLSSGFAANDD418 pKa = 3.96TITVNGKK425 pKa = 8.17TITFSTTAATSTSASGGVINLGTGTVQDD453 pKa = 3.8VLTAIDD459 pKa = 4.85EE460 pKa = 4.49ISGTTTPSTISASGVITLNDD480 pKa = 3.38NAGSLSITSSNSSALGALGFSASTITTPSALSASFSAGDD519 pKa = 3.64TVTVDD524 pKa = 3.07GKK526 pKa = 9.37TISFYY531 pKa = 11.3DD532 pKa = 3.71PTSTTGPTSAGSATNTTYY550 pKa = 11.49LNLATATVGTLLTTIDD566 pKa = 4.18SLTGASVHH574 pKa = 6.27ASISATGAITLNTGTAADD592 pKa = 4.49LSVSSTNTSAFGALGFTDD610 pKa = 4.17PMTAARR616 pKa = 11.84TGGGTAGTGVVIGNDD631 pKa = 2.9LTAFTNEE638 pKa = 4.49SISGGAVTAYY648 pKa = 10.47NSAGTPVNLQVRR660 pKa = 11.84WALTSTAGGQDD671 pKa = 3.53TWNMFYY677 pKa = 9.38QTDD680 pKa = 3.59TSATGSQVAWVNAGTNFVFNANGSLSSPSGSAITIPGVTVNNQSLGNLTLNVGEE734 pKa = 4.63GALTQYY740 pKa = 11.64ASTSGSATVNTFTQNGYY757 pKa = 10.11AAGQLQSVAINNNGLVVGTFSNGQDD782 pKa = 3.69LDD784 pKa = 4.06LASITLSHH792 pKa = 7.23FNGTNYY798 pKa = 10.22LQALSGEE805 pKa = 4.55AYY807 pKa = 10.29AVTAQSGPAIAGASGTISGSSLEE830 pKa = 4.39GSNTDD835 pKa = 3.49IADD838 pKa = 3.88EE839 pKa = 4.17FTKK842 pKa = 11.09LIVTQQAYY850 pKa = 9.83SANTKK855 pKa = 10.44VITTANDD862 pKa = 3.71MIQSLLSVLRR872 pKa = 4.12

Molecular weight: 85.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M8ZP66|A0A2M8ZP66_9BRAD DNA-binding transcriptional LysR family regulator OS=Afipia broomeae OX=56946 GN=BKA77_4155 PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 10.25VRR4 pKa = 11.84NSLKK8 pKa = 10.15SLRR11 pKa = 11.84GRR13 pKa = 11.84HH14 pKa = 5.06RR15 pKa = 11.84NNRR18 pKa = 11.84LVRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 10.2GRR25 pKa = 11.84VYY27 pKa = 10.74VINKK31 pKa = 4.67VQRR34 pKa = 11.84RR35 pKa = 11.84FKK37 pKa = 10.83ARR39 pKa = 11.84QGG41 pKa = 3.34

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7389

0

7389

2250058

29

2793

304.5

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.017 ± 0.038

0.884 ± 0.009

5.49 ± 0.021

5.135 ± 0.025

3.913 ± 0.017

8.314 ± 0.026

2.048 ± 0.015

5.599 ± 0.02

3.709 ± 0.026

9.89 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.012

2.885 ± 0.019

5.251 ± 0.023

3.179 ± 0.016

6.704 ± 0.03

5.926 ± 0.024

5.412 ± 0.023

7.481 ± 0.023

1.335 ± 0.011

2.342 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski