Desulfurococcaceae archaeon AG1

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; unclassified Desulfurococcaceae

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1903 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4ZRB5|A0A2R4ZRB5_9CREN Uncharacterized protein OS=Desulfurococcaceae archaeon AG1 OX=2027460 GN=ATG_03450 PE=4 SV=1
AA1 pKa = 7.52TEE3 pKa = 5.31PIATMRR9 pKa = 11.84VCRR12 pKa = 11.84NNVCEE17 pKa = 4.49AIGVSFAKK25 pKa = 9.46ATGWGWVDD33 pKa = 2.52IDD35 pKa = 3.89YY36 pKa = 10.89SYY38 pKa = 11.73SPEE41 pKa = 4.38LMDD44 pKa = 5.58IDD46 pKa = 4.06TLEE49 pKa = 4.31DD50 pKa = 3.46LLRR53 pKa = 11.84AEE55 pKa = 5.1EE56 pKa = 4.35ICGSMVEE63 pKa = 4.48GG64 pKa = 4.14

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4ZSV9|A0A2R4ZSV9_9CREN Threonine synthase OS=Desulfurococcaceae archaeon AG1 OX=2027460 GN=ATG_06880 PE=3 SV=1
MM1 pKa = 7.1PTHH4 pKa = 6.96GSMTKK9 pKa = 10.12AGKK12 pKa = 9.43VRR14 pKa = 11.84NATPKK19 pKa = 10.12IPPKK23 pKa = 9.87PKK25 pKa = 9.4RR26 pKa = 11.84NLVPRR31 pKa = 11.84VRR33 pKa = 11.84NRR35 pKa = 11.84RR36 pKa = 11.84EE37 pKa = 3.17FWIRR41 pKa = 11.84NRR43 pKa = 11.84KK44 pKa = 8.41AAGLPVPTVVPPSSVPRR61 pKa = 11.84KK62 pKa = 9.48KK63 pKa = 10.31QQ64 pKa = 3.02

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1903

0

1903

555226

39

2169

291.8

32.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.513 ± 0.053

0.686 ± 0.017

4.858 ± 0.039

7.048 ± 0.063

3.137 ± 0.033

7.802 ± 0.051

1.495 ± 0.023

9.322 ± 0.057

5.298 ± 0.052

10.063 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.026

2.781 ± 0.033

4.537 ± 0.037

1.696 ± 0.028

7.059 ± 0.046

7.074 ± 0.054

4.239 ± 0.044

7.781 ± 0.045

1.133 ± 0.02

4.057 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski