Ursus americanus (American black bear) (Euarctos americanus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A452RLE4|A0A452RLE4_URSAM Isoform of A0A452RLJ5 Peptidyl-prolyl cis-trans isomerase OS=Ursus americanus OX=9643 GN=PPIC PE=3 SV=1
MM1 pKa = 7.29EE2 pKa = 4.36VVVLIPYY9 pKa = 10.0VLGLLLLPLLAVALCVRR26 pKa = 11.84CRR28 pKa = 11.84EE29 pKa = 4.18MPGSYY34 pKa = 8.87DD35 pKa = 3.14TGASDD40 pKa = 4.59SLTPSIMLKK49 pKa = 10.24RR50 pKa = 11.84PPTVVPWPSATSYY63 pKa = 11.6APVTSYY69 pKa = 11.21PPLSQPDD76 pKa = 4.23LLPIPRR82 pKa = 11.84SPQPHH87 pKa = 6.67RR88 pKa = 11.84MPSSRR93 pKa = 11.84QDD95 pKa = 2.92SDD97 pKa = 3.47GANSVASYY105 pKa = 10.71EE106 pKa = 4.2NEE108 pKa = 3.86EE109 pKa = 4.31PACEE113 pKa = 5.26DD114 pKa = 3.82DD115 pKa = 6.45DD116 pKa = 5.13EE117 pKa = 7.07DD118 pKa = 4.93EE119 pKa = 4.31EE120 pKa = 5.84DD121 pKa = 3.83YY122 pKa = 11.55HH123 pKa = 8.47NEE125 pKa = 4.01GYY127 pKa = 11.01LEE129 pKa = 4.22VLPDD133 pKa = 3.67STPATSTAVAPAPVPSNPGLRR154 pKa = 11.84DD155 pKa = 3.14SAFSMEE161 pKa = 4.23SGEE164 pKa = 4.92DD165 pKa = 3.63YY166 pKa = 11.71VNVAEE171 pKa = 4.65SEE173 pKa = 4.22EE174 pKa = 4.29SADD177 pKa = 3.6VSLDD181 pKa = 3.29GSRR184 pKa = 11.84EE185 pKa = 3.96YY186 pKa = 11.72VNVSQEE192 pKa = 4.44LPPMARR198 pKa = 11.84TEE200 pKa = 3.92AAILNSQKK208 pKa = 10.22VEE210 pKa = 4.2DD211 pKa = 4.43EE212 pKa = 4.21DD213 pKa = 5.59DD214 pKa = 3.99EE215 pKa = 4.77EE216 pKa = 5.11EE217 pKa = 4.32EE218 pKa = 4.72GAPDD222 pKa = 3.77YY223 pKa = 11.52EE224 pKa = 4.36NLQGLHH230 pKa = 6.14

Molecular weight: 24.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A452QCH8|A0A452QCH8_URSAM Coiled-coil domain containing 148 OS=Ursus americanus OX=9643 GN=CCDC148 PE=4 SV=1
PP1 pKa = 7.66ASPAGAPSLGASRR14 pKa = 11.84RR15 pKa = 11.84AAPRR19 pKa = 11.84WSAATPRR26 pKa = 11.84RR27 pKa = 11.84TTTTAAPSPPTPGAPRR43 pKa = 11.84RR44 pKa = 11.84PATRR48 pKa = 11.84SPPPRR53 pKa = 11.84LTTTTSSPPRR63 pKa = 11.84GARR66 pKa = 11.84RR67 pKa = 11.84AARR70 pKa = 11.84AARR73 pKa = 11.84AASRR77 pKa = 11.84ATRR80 pKa = 11.84ATARR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84TTANTAPRR95 pKa = 11.84RR96 pKa = 11.84PRR98 pKa = 11.84RR99 pKa = 11.84PWGTAATRR107 pKa = 11.84PSTRR111 pKa = 11.84SASTSPRR118 pKa = 11.84PGRR121 pKa = 11.84CPATRR126 pKa = 11.84CPSKK130 pKa = 10.47RR131 pKa = 11.84DD132 pKa = 3.36ARR134 pKa = 11.84RR135 pKa = 11.84SPPRR139 pKa = 11.84ATTRR143 pKa = 11.84RR144 pKa = 3.62

Molecular weight: 15.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20406

12106

32512

18648622

27

7814

573.6

63.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.972 ± 0.013

2.302 ± 0.014

4.829 ± 0.012

7.001 ± 0.018

3.754 ± 0.009

6.549 ± 0.018

2.579 ± 0.006

4.39 ± 0.011

5.623 ± 0.019

10.096 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.005

3.604 ± 0.009

6.169 ± 0.021

4.707 ± 0.012

5.712 ± 0.012

8.196 ± 0.016

5.239 ± 0.009

6.18 ± 0.011

1.224 ± 0.005

2.689 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski