Streptomyces phage Forthebois

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Deltatectivirus; Streptomyces virus Forthebois

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E2V8|A0A4D6E2V8_9VIRU Uncharacterized protein OS=Streptomyces phage Forthebois OX=2562185 GN=1 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.8FGNPIVGGEE11 pKa = 3.96DD12 pKa = 4.54LIRR15 pKa = 11.84TAIKK19 pKa = 10.81SPDD22 pKa = 3.62FNTDD26 pKa = 3.38PEE28 pKa = 4.61SGNVTGWRR36 pKa = 11.84IARR39 pKa = 11.84DD40 pKa = 3.76GSATFYY46 pKa = 11.33NLTIGSADD54 pKa = 3.58FNIDD58 pKa = 3.25EE59 pKa = 4.75NGNAVFQSVTANDD72 pKa = 3.16ITLDD76 pKa = 3.83GEE78 pKa = 4.23NLEE81 pKa = 4.41SVLAEE86 pKa = 4.13KK87 pKa = 10.82ALGTLAIVTLSGDD100 pKa = 3.41TAGYY104 pKa = 10.52NGTSLLFGRR113 pKa = 11.84IVIPNFDD120 pKa = 3.77GTRR123 pKa = 11.84QYY125 pKa = 11.5AIGGSGVHH133 pKa = 6.44FDD135 pKa = 3.56KK136 pKa = 11.23QAVTGFTRR144 pKa = 11.84LTIRR148 pKa = 11.84AYY150 pKa = 10.57LAWDD154 pKa = 4.2TPATTASTQLVEE166 pKa = 4.05YY167 pKa = 10.44QYY169 pKa = 11.62LSDD172 pKa = 4.06SASGSDD178 pKa = 3.34WIVSFRR184 pKa = 11.84HH185 pKa = 5.97LFQDD189 pKa = 3.89SSPAGTDD196 pKa = 3.2AHH198 pKa = 6.17IAWYY202 pKa = 7.81FTTNVNNTAGLRR214 pKa = 11.84CSGYY218 pKa = 10.64DD219 pKa = 3.34SGGGTPGSRR228 pKa = 11.84IYY230 pKa = 11.2AEE232 pKa = 4.18DD233 pKa = 3.62AGDD236 pKa = 3.26IVTYY240 pKa = 10.58TDD242 pKa = 3.78FNMGGGGGTPVQSYY256 pKa = 8.24TKK258 pKa = 9.38TYY260 pKa = 10.08AANEE264 pKa = 4.25SASYY268 pKa = 9.62QQDD271 pKa = 2.87GSNRR275 pKa = 11.84GIADD279 pKa = 4.53CYY281 pKa = 8.67QGRR284 pKa = 11.84YY285 pKa = 8.86SATNGNQYY293 pKa = 11.65SMIGFDD299 pKa = 3.79DD300 pKa = 3.96AQIRR304 pKa = 11.84SDD306 pKa = 3.83LSGATITKK314 pKa = 10.09VEE316 pKa = 4.29LYY318 pKa = 10.8LNNNHH323 pKa = 6.41FYY325 pKa = 11.25SNSGGNAVIGTHH337 pKa = 5.49NQTTLSGSHH346 pKa = 6.46SSSQINDD353 pKa = 3.29NLQQTHH359 pKa = 6.59FDD361 pKa = 3.43LGQAKK366 pKa = 9.85WITIPNSIGNALRR379 pKa = 11.84DD380 pKa = 3.75NTAKK384 pKa = 10.89GIALGPGPTTSQSYY398 pKa = 9.76YY399 pKa = 11.02GYY401 pKa = 10.39FAGNGQSGEE410 pKa = 3.96PQLRR414 pKa = 11.84ITYY417 pKa = 8.08TKK419 pKa = 10.94

Molecular weight: 44.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E2V9|A0A4D6E2V9_9VIRU Uncharacterized protein OS=Streptomyces phage Forthebois OX=2562185 GN=9 PE=4 SV=1
MM1 pKa = 7.58AAQRR5 pKa = 11.84SMKK8 pKa = 10.16DD9 pKa = 2.82KK10 pKa = 10.85MMAAMLKK17 pKa = 10.43DD18 pKa = 3.26KK19 pKa = 11.14GIARR23 pKa = 11.84TTGQCPRR30 pKa = 11.84CHH32 pKa = 6.45KK33 pKa = 10.12FAYY36 pKa = 10.04SIKK39 pKa = 9.62NQNSLILHH47 pKa = 6.65LNGCQGRR54 pKa = 11.84RR55 pKa = 11.84AKK57 pKa = 10.95VNN59 pKa = 3.23

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

5544

38

700

154.0

16.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.011 ± 0.622

0.685 ± 0.18

5.592 ± 0.281

4.96 ± 0.475

3.481 ± 0.228

9.578 ± 0.647

1.641 ± 0.189

5.195 ± 0.367

5.213 ± 0.47

6.836 ± 0.528

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.36

4.816 ± 0.376

5.628 ± 0.558

3.535 ± 0.384

5.014 ± 0.544

6.025 ± 0.388

7.143 ± 0.545

6.854 ± 0.59

1.858 ± 0.206

3.12 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski