Ustilago trichophora
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13002 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C3DYT7|A0A5C3DYT7_9BASI Related to LST8 - required for transport of permeases from the golgi to the plasma membrane OS=Ustilago trichophora OX=86804 GN=UTRI_03848_B PE=3 SV=1
MM1 pKa = 6.93 YY2 pKa = 9.56 QACVNFDD9 pKa = 4.43 SINCDD14 pKa = 3.06 QHH16 pKa = 8.18 DD17 pKa = 4.15 VASAASVPILPSSNWLVDD35 pKa = 3.52 AANEE39 pKa = 4.01 LHH41 pKa = 6.87 SEE43 pKa = 4.27 YY44 pKa = 10.34 PPEE47 pKa = 4.6 TPHH50 pKa = 5.42 QTNNAPMEE58 pKa = 4.56 PCEE61 pKa = 4.38 HH62 pKa = 7.19 LDD64 pKa = 3.75 LFDD67 pKa = 4.53 SASVDD72 pKa = 5.11 DD73 pKa = 5.92 NDD75 pKa = 6.3 DD76 pKa = 5.0 DD77 pKa = 7.12 DD78 pKa = 7.36 DD79 pKa = 7.34 DD80 pKa = 6.93 DD81 pKa = 6.67 DD82 pKa = 4.75 EE83 pKa = 6.9 SEE85 pKa = 6.36 LLACWDD91 pKa = 3.84 SQDD94 pKa = 4.59 EE95 pKa = 4.41 YY96 pKa = 11.34 NDD98 pKa = 3.43 NDD100 pKa = 4.07 GATTPVQFGVEE111 pKa = 4.21 HH112 pKa = 6.42 QDD114 pKa = 2.96 EE115 pKa = 4.63 SQTFEE120 pKa = 3.79 EE121 pKa = 4.42 QQRR124 pKa = 11.84 IQQPMPASSQSAEE137 pKa = 3.8 IVSSNTDD144 pKa = 2.88 EE145 pKa = 4.7 TIPRR149 pKa = 11.84 TRR151 pKa = 11.84 RR152 pKa = 11.84 RR153 pKa = 11.84 CMAEE157 pKa = 3.41 IARR160 pKa = 11.84 TLAIHH165 pKa = 6.42 MGMASIAFGLGCLSTTVRR183 pKa = 11.84 TAAPNPSQTFSVDD196 pKa = 3.14 KK197 pKa = 10.84 LLQLALNHH205 pKa = 6.57 LAA207 pKa = 4.66
Molecular weight: 22.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.872
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.888
Protein with the highest isoelectric point:
>tr|A0A5C3EBZ5|A0A5C3EBZ5_9BASI alpha-1 2-Mannosidase OS=Ustilago trichophora OX=86804 GN=UTRI_04591_B PE=3 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.12 WRR6 pKa = 11.84 KK7 pKa = 8.88 KK8 pKa = 8.94 RR9 pKa = 11.84 MRR11 pKa = 11.84 RR12 pKa = 11.84 LRR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 8.14 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.51 MRR21 pKa = 11.84 ARR23 pKa = 11.84 SKK25 pKa = 11.11
Molecular weight: 3.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13002
0
13002
7812304
25
4858
600.9
65.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.266 ± 0.027
0.963 ± 0.007
5.692 ± 0.014
5.71 ± 0.019
3.251 ± 0.012
6.816 ± 0.024
2.494 ± 0.011
4.135 ± 0.014
4.634 ± 0.019
8.744 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.929 ± 0.007
3.427 ± 0.012
6.23 ± 0.019
4.345 ± 0.019
6.259 ± 0.017
10.042 ± 0.034
6.013 ± 0.013
5.641 ± 0.016
1.176 ± 0.007
2.233 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here