Amino acid dipepetide frequency for Streptomyces sp. SID10815

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.739AlaAla: 22.739 ± 0.13
1.141AlaCys: 1.141 ± 0.027
8.917AlaAsp: 8.917 ± 0.075
8.978AlaGlu: 8.978 ± 0.083
3.614AlaPhe: 3.614 ± 0.042
13.315AlaGly: 13.315 ± 0.094
3.306AlaHis: 3.306 ± 0.045
3.226AlaIle: 3.226 ± 0.042
2.873AlaLys: 2.873 ± 0.056
15.126AlaLeu: 15.126 ± 0.105
2.441AlaMet: 2.441 ± 0.036
1.912AlaAsn: 1.912 ± 0.037
7.362AlaPro: 7.362 ± 0.078
3.842AlaGln: 3.842 ± 0.041
10.981AlaArg: 10.981 ± 0.088
5.695AlaSer: 5.695 ± 0.055
6.994AlaThr: 6.994 ± 0.061
12.663AlaVal: 12.663 ± 0.095
1.902AlaTrp: 1.902 ± 0.031
2.942AlaTyr: 2.942 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.186CysAla: 1.186 ± 0.027
0.092CysCys: 0.092 ± 0.007
0.518CysAsp: 0.518 ± 0.014
0.398CysGlu: 0.398 ± 0.014
0.21CysPhe: 0.21 ± 0.01
0.977CysGly: 0.977 ± 0.023
0.209CysHis: 0.209 ± 0.01
0.135CysIle: 0.135 ± 0.009
0.117CysLys: 0.117 ± 0.008
0.805CysLeu: 0.805 ± 0.018
0.119CysMet: 0.119 ± 0.008
0.133CysAsn: 0.133 ± 0.008
0.513CysPro: 0.513 ± 0.017
0.18CysGln: 0.18 ± 0.01
0.632CysArg: 0.632 ± 0.02
0.437CysSer: 0.437 ± 0.015
0.518CysThr: 0.518 ± 0.016
0.682CysVal: 0.682 ± 0.018
0.131CysTrp: 0.131 ± 0.008
0.144CysTyr: 0.144 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
8.225AspAla: 8.225 ± 0.07
0.437AspCys: 0.437 ± 0.014
3.734AspAsp: 3.734 ± 0.043
3.788AspGlu: 3.788 ± 0.047
1.683AspPhe: 1.683 ± 0.027
6.521AspGly: 6.521 ± 0.063
1.515AspHis: 1.515 ± 0.029
1.705AspIle: 1.705 ± 0.029
1.191AspLys: 1.191 ± 0.031
6.425AspLeu: 6.425 ± 0.065
0.802AspMet: 0.802 ± 0.018
0.922AspAsn: 0.922 ± 0.02
4.567AspPro: 4.567 ± 0.048
1.567AspGln: 1.567 ± 0.028
5.218AspArg: 5.218 ± 0.053
2.482AspSer: 2.482 ± 0.031
3.337AspThr: 3.337 ± 0.041
4.949AspVal: 4.949 ± 0.051
1.067AspTrp: 1.067 ± 0.022
1.139AspTyr: 1.139 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.172GluAla: 7.172 ± 0.073
0.369GluCys: 0.369 ± 0.013
2.888GluAsp: 2.888 ± 0.037
3.507GluGlu: 3.507 ± 0.054
1.431GluPhe: 1.431 ± 0.026
4.152GluGly: 4.152 ± 0.047
1.578GluHis: 1.578 ± 0.03
2.208GluIle: 2.208 ± 0.032
1.324GluLys: 1.324 ± 0.026
6.77GluLeu: 6.77 ± 0.063
0.818GluMet: 0.818 ± 0.02
0.973GluAsn: 0.973 ± 0.023
3.261GluPro: 3.261 ± 0.041
2.161GluGln: 2.161 ± 0.035
5.736GluArg: 5.736 ± 0.059
2.389GluSer: 2.389 ± 0.034
2.855GluThr: 2.855 ± 0.036
4.382GluVal: 4.382 ± 0.05
0.733GluTrp: 0.733 ± 0.019
1.079GluTyr: 1.079 ± 0.02
0.0GluXaa: 0.0 ± 0.0
Phe
3.813PheAla: 3.813 ± 0.05
0.272PheCys: 0.272 ± 0.012
2.002PheAsp: 2.002 ± 0.037
1.384PheGlu: 1.384 ± 0.026
0.856PhePhe: 0.856 ± 0.023
2.953PheGly: 2.953 ± 0.038
0.65PheHis: 0.65 ± 0.017
0.63PheIle: 0.63 ± 0.017
0.496PheLys: 0.496 ± 0.016
2.574PheLeu: 2.574 ± 0.04
0.376PheMet: 0.376 ± 0.013
0.524PheAsn: 0.524 ± 0.016
1.381PhePro: 1.381 ± 0.024
0.71PheGln: 0.71 ± 0.02
1.86PheArg: 1.86 ± 0.031
1.407PheSer: 1.407 ± 0.026
2.021PheThr: 2.021 ± 0.034
2.246PheVal: 2.246 ± 0.037
0.397PheTrp: 0.397 ± 0.014
0.579PheTyr: 0.579 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
11.735GlyAla: 11.735 ± 0.084
0.847GlyCys: 0.847 ± 0.02
5.326GlyAsp: 5.326 ± 0.049
4.96GlyGlu: 4.96 ± 0.056
2.811GlyPhe: 2.811 ± 0.041
8.936GlyGly: 8.936 ± 0.1
2.508GlyHis: 2.508 ± 0.036
3.09GlyIle: 3.09 ± 0.041
2.293GlyLys: 2.293 ± 0.04
9.264GlyLeu: 9.264 ± 0.071
1.901GlyMet: 1.901 ± 0.033
1.671GlyAsn: 1.671 ± 0.035
5.071GlyPro: 5.071 ± 0.058
2.663GlyGln: 2.663 ± 0.042
8.13GlyArg: 8.13 ± 0.063
5.095GlySer: 5.095 ± 0.06
5.974GlyThr: 5.974 ± 0.06
7.793GlyVal: 7.793 ± 0.078
1.691GlyTrp: 1.691 ± 0.027
2.307GlyTyr: 2.307 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.832HisAla: 2.832 ± 0.037
0.23HisCys: 0.23 ± 0.01
1.435HisAsp: 1.435 ± 0.026
1.263HisGlu: 1.263 ± 0.027
0.649HisPhe: 0.649 ± 0.019
2.596HisGly: 2.596 ± 0.038
0.767HisHis: 0.767 ± 0.025
0.652HisIle: 0.652 ± 0.019
0.37HisLys: 0.37 ± 0.014
2.565HisLeu: 2.565 ± 0.037
0.32HisMet: 0.32 ± 0.012
0.365HisAsn: 0.365 ± 0.013
1.946HisPro: 1.946 ± 0.036
0.663HisGln: 0.663 ± 0.019
2.243HisArg: 2.243 ± 0.035
1.056HisSer: 1.056 ± 0.025
1.476HisThr: 1.476 ± 0.03
1.779HisVal: 1.779 ± 0.028
0.417HisTrp: 0.417 ± 0.013
0.496HisTyr: 0.496 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.488IleAla: 4.488 ± 0.048
0.255IleCys: 0.255 ± 0.012
2.137IleAsp: 2.137 ± 0.031
1.847IleGlu: 1.847 ± 0.031
0.596IlePhe: 0.596 ± 0.017
3.349IleGly: 3.349 ± 0.046
0.569IleHis: 0.569 ± 0.018
0.762IleIle: 0.762 ± 0.02
0.697IleLys: 0.697 ± 0.019
2.254IleLeu: 2.254 ± 0.037
0.408IleMet: 0.408 ± 0.013
0.635IleAsn: 0.635 ± 0.019
1.575IlePro: 1.575 ± 0.03
0.667IleGln: 0.667 ± 0.017
2.127IleArg: 2.127 ± 0.033
1.522IleSer: 1.522 ± 0.026
2.08IleThr: 2.08 ± 0.036
2.617IleVal: 2.617 ± 0.033
0.346IleTrp: 0.346 ± 0.013
0.48IleTyr: 0.48 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.887LysAla: 2.887 ± 0.047
0.123LysCys: 0.123 ± 0.007
1.304LysAsp: 1.304 ± 0.03
1.112LysGlu: 1.112 ± 0.026
0.465LysPhe: 0.465 ± 0.015
1.799LysGly: 1.799 ± 0.031
0.392LysHis: 0.392 ± 0.013
0.846LysIle: 0.846 ± 0.02
0.843LysLys: 0.843 ± 0.031
1.961LysLeu: 1.961 ± 0.033
0.347LysMet: 0.347 ± 0.015
0.528LysAsn: 0.528 ± 0.016
1.225LysPro: 1.225 ± 0.027
0.698LysGln: 0.698 ± 0.019
1.396LysArg: 1.396 ± 0.026
1.14LysSer: 1.14 ± 0.027
1.285LysThr: 1.285 ± 0.032
1.844LysVal: 1.844 ± 0.033
0.265LysTrp: 0.265 ± 0.011
0.464LysTyr: 0.464 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
15.528LeuAla: 15.528 ± 0.115
0.898LeuCys: 0.898 ± 0.022
6.92LeuAsp: 6.92 ± 0.054
4.661LeuGlu: 4.661 ± 0.052
2.636LeuPhe: 2.636 ± 0.043
9.097LeuGly: 9.097 ± 0.081
2.37LeuHis: 2.37 ± 0.033
3.135LeuIle: 3.135 ± 0.044
2.061LeuLys: 2.061 ± 0.034
11.309LeuLeu: 11.309 ± 0.098
1.614LeuMet: 1.614 ± 0.03
1.633LeuAsn: 1.633 ± 0.03
6.678LeuPro: 6.678 ± 0.062
2.175LeuGln: 2.175 ± 0.036
8.916LeuArg: 8.916 ± 0.076
5.197LeuSer: 5.197 ± 0.044
7.182LeuThr: 7.182 ± 0.062
8.827LeuVal: 8.827 ± 0.08
1.319LeuTrp: 1.319 ± 0.03
1.922LeuTyr: 1.922 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.215MetAla: 2.215 ± 0.031
0.134MetCys: 0.134 ± 0.008
0.873MetAsp: 0.873 ± 0.022
0.702MetGlu: 0.702 ± 0.021
0.432MetPhe: 0.432 ± 0.015
1.253MetGly: 1.253 ± 0.026
0.332MetHis: 0.332 ± 0.011
0.604MetIle: 0.604 ± 0.017
0.401MetLys: 0.401 ± 0.013
1.559MetLeu: 1.559 ± 0.031
0.268MetMet: 0.268 ± 0.012
0.419MetAsn: 0.419 ± 0.014
1.047MetPro: 1.047 ± 0.023
0.407MetGln: 0.407 ± 0.014
1.35MetArg: 1.35 ± 0.026
1.29MetSer: 1.29 ± 0.022
1.496MetThr: 1.496 ± 0.028
1.211MetVal: 1.211 ± 0.025
0.202MetTrp: 0.202 ± 0.008
0.322MetTyr: 0.322 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.123AsnAla: 2.123 ± 0.033
0.149AsnCys: 0.149 ± 0.008
0.906AsnAsp: 0.906 ± 0.022
0.774AsnGlu: 0.774 ± 0.022
0.458AsnPhe: 0.458 ± 0.014
1.848AsnGly: 1.848 ± 0.04
0.376AsnHis: 0.376 ± 0.013
0.581AsnIle: 0.581 ± 0.016
0.411AsnLys: 0.411 ± 0.014
1.567AsnLeu: 1.567 ± 0.029
0.283AsnMet: 0.283 ± 0.013
0.383AsnAsn: 0.383 ± 0.013
1.283AsnPro: 1.283 ± 0.024
0.494AsnGln: 0.494 ± 0.018
1.252AsnArg: 1.252 ± 0.028
0.887AsnSer: 0.887 ± 0.025
1.089AsnThr: 1.089 ± 0.024
1.346AsnVal: 1.346 ± 0.03
0.27AsnTrp: 0.27 ± 0.013
0.404AsnTyr: 0.404 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
9.094ProAla: 9.094 ± 0.081
0.338ProCys: 0.338 ± 0.013
4.637ProAsp: 4.637 ± 0.05
4.303ProGlu: 4.303 ± 0.05
1.543ProPhe: 1.543 ± 0.025
6.497ProGly: 6.497 ± 0.063
1.514ProHis: 1.514 ± 0.025
1.198ProIle: 1.198 ± 0.027
1.144ProLys: 1.144 ± 0.026
5.419ProLeu: 5.419 ± 0.056
0.968ProMet: 0.968 ± 0.022
0.861ProAsn: 0.861 ± 0.023
3.433ProPro: 3.433 ± 0.054
1.648ProGln: 1.648 ± 0.035
4.166ProArg: 4.166 ± 0.053
2.969ProSer: 2.969 ± 0.042
3.067ProThr: 3.067 ± 0.044
5.415ProVal: 5.415 ± 0.055
0.874ProTrp: 0.874 ± 0.023
1.439ProTyr: 1.439 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.684GlnAla: 3.684 ± 0.046
0.17GlnCys: 0.17 ± 0.009
1.438GlnAsp: 1.438 ± 0.03
1.387GlnGlu: 1.387 ± 0.026
0.673GlnPhe: 0.673 ± 0.017
2.298GlnGly: 2.298 ± 0.032
0.678GlnHis: 0.678 ± 0.018
1.077GlnIle: 1.077 ± 0.022
0.634GlnLys: 0.634 ± 0.019
2.968GlnLeu: 2.968 ± 0.04
0.446GlnMet: 0.446 ± 0.015
0.514GlnAsn: 0.514 ± 0.018
1.621GlnPro: 1.621 ± 0.04
1.22GlnGln: 1.22 ± 0.028
2.368GlnArg: 2.368 ± 0.033
1.262GlnSer: 1.262 ± 0.025
1.318GlnThr: 1.318 ± 0.025
2.38GlnVal: 2.38 ± 0.036
0.473GlnTrp: 0.473 ± 0.015
0.607GlnTyr: 0.607 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
10.515ArgAla: 10.515 ± 0.09
0.619ArgCys: 0.619 ± 0.017
4.513ArgAsp: 4.513 ± 0.048
4.819ArgGlu: 4.819 ± 0.056
2.372ArgPhe: 2.372 ± 0.035
5.915ArgGly: 5.915 ± 0.053
2.267ArgHis: 2.267 ± 0.037
3.092ArgIle: 3.092 ± 0.039
1.539ArgLys: 1.539 ± 0.029
9.477ArgLeu: 9.477 ± 0.085
1.696ArgMet: 1.696 ± 0.025
1.255ArgAsn: 1.255 ± 0.022
5.314ArgPro: 5.314 ± 0.058
2.327ArgGln: 2.327 ± 0.031
8.096ArgArg: 8.096 ± 0.082
3.859ArgSer: 3.859 ± 0.043
5.691ArgThr: 5.691 ± 0.056
6.257ArgVal: 6.257 ± 0.064
1.375ArgTrp: 1.375 ± 0.027
1.824ArgTyr: 1.824 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
6.782SerAla: 6.782 ± 0.064
0.416SerCys: 0.416 ± 0.013
2.595SerAsp: 2.595 ± 0.034
2.16SerGlu: 2.16 ± 0.033
1.492SerPhe: 1.492 ± 0.026
5.839SerGly: 5.839 ± 0.07
0.983SerHis: 0.983 ± 0.023
1.273SerIle: 1.273 ± 0.025
0.947SerLys: 0.947 ± 0.024
4.766SerLeu: 4.766 ± 0.052
0.968SerMet: 0.968 ± 0.019
0.766SerAsn: 0.766 ± 0.02
3.007SerPro: 3.007 ± 0.037
1.188SerGln: 1.188 ± 0.025
3.482SerArg: 3.482 ± 0.042
2.579SerSer: 2.579 ± 0.053
2.899SerThr: 2.899 ± 0.044
4.193SerVal: 4.193 ± 0.044
0.868SerTrp: 0.868 ± 0.022
1.189SerTyr: 1.189 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.441ThrAla: 9.441 ± 0.075
0.454ThrCys: 0.454 ± 0.016
3.652ThrAsp: 3.652 ± 0.043
3.18ThrGlu: 3.18 ± 0.041
1.564ThrPhe: 1.564 ± 0.027
6.904ThrGly: 6.904 ± 0.066
1.247ThrHis: 1.247 ± 0.024
1.526ThrIle: 1.526 ± 0.029
1.066ThrLys: 1.066 ± 0.026
5.732ThrLeu: 5.732 ± 0.054
0.885ThrMet: 0.885 ± 0.018
0.941ThrAsn: 0.941 ± 0.026
4.058ThrPro: 4.058 ± 0.046
1.305ThrGln: 1.305 ± 0.028
3.995ThrArg: 3.995 ± 0.037
3.005ThrSer: 3.005 ± 0.04
3.702ThrThr: 3.702 ± 0.056
6.229ThrVal: 6.229 ± 0.059
0.881ThrTrp: 0.881 ± 0.023
1.319ThrTyr: 1.319 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.943ValAla: 10.943 ± 0.088
0.845ValCys: 0.845 ± 0.021
5.096ValAsp: 5.096 ± 0.051
4.639ValGlu: 4.639 ± 0.058
2.485ValPhe: 2.485 ± 0.038
6.463ValGly: 6.463 ± 0.066
2.068ValHis: 2.068 ± 0.032
2.719ValIle: 2.719 ± 0.035
1.757ValLys: 1.757 ± 0.036
9.662ValLeu: 9.662 ± 0.076
1.341ValMet: 1.341 ± 0.026
1.628ValAsn: 1.628 ± 0.029
5.308ValPro: 5.308 ± 0.053
2.071ValGln: 2.071 ± 0.029
7.617ValArg: 7.617 ± 0.072
4.225ValSer: 4.225 ± 0.045
5.804ValThr: 5.804 ± 0.057
8.051ValVal: 8.051 ± 0.075
1.146ValTrp: 1.146 ± 0.024
1.644ValTyr: 1.644 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.637TrpAla: 1.637 ± 0.03
0.172TrpCys: 0.172 ± 0.009
0.843TrpAsp: 0.843 ± 0.021
0.718TrpGlu: 0.718 ± 0.021
0.512TrpPhe: 0.512 ± 0.017
1.056TrpGly: 1.056 ± 0.021
0.387TrpHis: 0.387 ± 0.014
0.517TrpIle: 0.517 ± 0.016
0.373TrpLys: 0.373 ± 0.014
1.75TrpLeu: 1.75 ± 0.031
0.281TrpMet: 0.281 ± 0.01
0.41TrpAsn: 0.41 ± 0.015
0.76TrpPro: 0.76 ± 0.02
0.609TrpGln: 0.609 ± 0.018
1.379TrpArg: 1.379 ± 0.026
0.963TrpSer: 0.963 ± 0.024
1.072TrpThr: 1.072 ± 0.02
0.925TrpVal: 0.925 ± 0.022
0.313TrpTrp: 0.313 ± 0.012
0.355TrpTyr: 0.355 ± 0.011
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.932TyrAla: 2.932 ± 0.038
0.176TyrCys: 0.176 ± 0.008
1.541TyrAsp: 1.541 ± 0.029
1.207TyrGlu: 1.207 ± 0.025
0.653TyrPhe: 0.653 ± 0.018
2.297TyrGly: 2.297 ± 0.039
0.402TyrHis: 0.402 ± 0.013
0.433TyrIle: 0.433 ± 0.016
0.386TyrLys: 0.386 ± 0.013
2.124TyrLeu: 2.124 ± 0.034
0.232TyrMet: 0.232 ± 0.01
0.41TyrAsn: 0.41 ± 0.016
1.119TyrPro: 1.119 ± 0.025
0.621TyrGln: 0.621 ± 0.017
1.879TyrArg: 1.879 ± 0.032
0.933TyrSer: 0.933 ± 0.022
1.226TyrThr: 1.226 ± 0.026
1.723TyrVal: 1.723 ± 0.029
0.362TyrTrp: 0.362 ± 0.013
0.464TyrTyr: 0.464 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7832 proteins (2222051 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski