Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ruegeria; Ruegeria pomeroyi

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5LNP8|Q5LNP8_RUEPO UPF0260 protein SPO3155 OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) OX=246200 GN=SPO3155 PE=3 SV=1
MM1 pKa = 7.43RR2 pKa = 11.84AAVRR6 pKa = 11.84LQLLPHH12 pKa = 6.62MDD14 pKa = 3.72SIYY17 pKa = 10.54FFCNLTACYY26 pKa = 10.44YY27 pKa = 11.06LLLNRR32 pKa = 11.84GTLVLGTILFAALGVAAIATVISSDD57 pKa = 3.72DD58 pKa = 3.76DD59 pKa = 3.78SHH61 pKa = 8.87EE62 pKa = 4.54EE63 pKa = 3.88IADD66 pKa = 3.66GDD68 pKa = 4.04VPPPIEE74 pKa = 4.83EE75 pKa = 4.08EE76 pKa = 4.32DD77 pKa = 3.83GTPSLIQEE85 pKa = 4.29NGLTVVTGNEE95 pKa = 4.0EE96 pKa = 3.73PVQIDD101 pKa = 3.74VEE103 pKa = 4.23ALDD106 pKa = 3.9EE107 pKa = 5.16DD108 pKa = 4.24GDD110 pKa = 3.88ARR112 pKa = 11.84VYY114 pKa = 10.43QGSEE118 pKa = 3.63ADD120 pKa = 3.41EE121 pKa = 4.22TVYY124 pKa = 10.54IDD126 pKa = 4.66PDD128 pKa = 3.83TTSEE132 pKa = 3.87FSILMGSGGADD143 pKa = 2.94TVVTGLGQNISTTDD157 pKa = 3.35GADD160 pKa = 3.34ADD162 pKa = 4.34AEE164 pKa = 4.23EE165 pKa = 5.18ADD167 pKa = 4.32TVRR170 pKa = 11.84LIYY173 pKa = 10.38TPGASEE179 pKa = 4.33EE180 pKa = 4.12LFEE183 pKa = 5.87DD184 pKa = 4.7GSVRR188 pKa = 11.84GSFSMDD194 pKa = 2.97KK195 pKa = 11.06NDD197 pKa = 3.52VLDD200 pKa = 3.6VQFPEE205 pKa = 5.03GEE207 pKa = 4.29SGTLLPVYY215 pKa = 9.42STFVSYY221 pKa = 10.32PRR223 pKa = 11.84DD224 pKa = 3.5ACCSFEE230 pKa = 4.26EE231 pKa = 4.33EE232 pKa = 4.49HH233 pKa = 7.0EE234 pKa = 4.22LTLVYY239 pKa = 10.38IPDD242 pKa = 3.49EE243 pKa = 4.3VEE245 pKa = 3.88YY246 pKa = 10.97SLEE249 pKa = 4.03EE250 pKa = 4.31LEE252 pKa = 4.48SSYY255 pKa = 11.14YY256 pKa = 10.29HH257 pKa = 7.01DD258 pKa = 3.83WEE260 pKa = 4.85ALGMVPIADD269 pKa = 3.69FHH271 pKa = 6.64LGQRR275 pKa = 11.84GEE277 pKa = 3.69IDD279 pKa = 3.46FTHH282 pKa = 6.6EE283 pKa = 4.27GEE285 pKa = 4.26SGEE288 pKa = 3.95FRR290 pKa = 11.84EE291 pKa = 4.02WDD293 pKa = 3.78YY294 pKa = 11.71VRR296 pKa = 11.84EE297 pKa = 4.21PPTVTSNLAIEE308 pKa = 4.12RR309 pKa = 11.84QILIRR314 pKa = 11.84YY315 pKa = 7.21

Molecular weight: 34.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5LL17|Q5LL17_RUEPO Nitric oxide reductase F protein putative OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) OX=246200 GN=SPOA0212 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 9.89PVRR5 pKa = 11.84PQPATPPALSEE16 pKa = 4.08NDD18 pKa = 3.41RR19 pKa = 11.84QALNDD24 pKa = 3.81LYY26 pKa = 11.73VRR28 pKa = 11.84GTPEE32 pKa = 3.5NTLRR36 pKa = 11.84AYY38 pKa = 9.92EE39 pKa = 4.3RR40 pKa = 11.84DD41 pKa = 3.82LLYY44 pKa = 10.1LTAWKK49 pKa = 9.83QLSFGAPLAWPEE61 pKa = 3.86QHH63 pKa = 6.25EE64 pKa = 4.39CALRR68 pKa = 11.84FILDD72 pKa = 3.62HH73 pKa = 7.16SRR75 pKa = 11.84DD76 pKa = 3.73LGDD79 pKa = 3.22ATGPARR85 pKa = 11.84EE86 pKa = 4.07VAEE89 pKa = 3.95ALIARR94 pKa = 11.84GLRR97 pKa = 11.84RR98 pKa = 11.84SLACPAPATLNRR110 pKa = 11.84RR111 pKa = 11.84IASWQAFHH119 pKa = 7.42RR120 pKa = 11.84MKK122 pKa = 10.98NLDD125 pKa = 3.79SPFDD129 pKa = 3.9SPLLKK134 pKa = 10.02QARR137 pKa = 11.84AKK139 pKa = 10.41ARR141 pKa = 11.84RR142 pKa = 11.84AAARR146 pKa = 11.84PTAPKK151 pKa = 10.0SRR153 pKa = 11.84NPITRR158 pKa = 11.84PVLEE162 pKa = 4.44QLLATCRR169 pKa = 11.84GSRR172 pKa = 11.84RR173 pKa = 11.84DD174 pKa = 3.53CRR176 pKa = 11.84DD177 pKa = 2.94RR178 pKa = 11.84ALLMLGWASGGRR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 11.84SEE194 pKa = 3.65IASLRR199 pKa = 11.84VEE201 pKa = 4.4DD202 pKa = 3.91VSLRR206 pKa = 11.84DD207 pKa = 3.63FAADD211 pKa = 4.81GIAWLMLVEE220 pKa = 4.64TKK222 pKa = 7.05TTRR225 pKa = 11.84RR226 pKa = 11.84GATPRR231 pKa = 11.84LVLKK235 pKa = 10.72GRR237 pKa = 11.84AAQALVHH244 pKa = 5.76WLEE247 pKa = 3.87VSGIEE252 pKa = 4.95DD253 pKa = 3.71GPLFRR258 pKa = 11.84PVSKK262 pKa = 10.35SDD264 pKa = 3.17RR265 pKa = 11.84VLTRR269 pKa = 11.84RR270 pKa = 11.84LTPDD274 pKa = 4.23AIYY277 pKa = 10.34QIVRR281 pKa = 11.84HH282 pKa = 5.82RR283 pKa = 11.84LQLAGLPQDD292 pKa = 3.74FASPHH297 pKa = 5.27GLRR300 pKa = 11.84SGFLTQAALDD310 pKa = 3.96GAPIQAAMRR319 pKa = 11.84LSLHH323 pKa = 6.57RR324 pKa = 11.84SVAQAQRR331 pKa = 11.84YY332 pKa = 8.12YY333 pKa = 11.22DD334 pKa = 3.45DD335 pKa = 4.36VEE337 pKa = 4.36IVEE340 pKa = 4.79NPATDD345 pKa = 3.67LLGG348 pKa = 4.09

Molecular weight: 38.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4269

0

4269

1375997

30

8093

322.3

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.523 ± 0.052

0.944 ± 0.012

5.919 ± 0.046

5.776 ± 0.033

3.648 ± 0.025

8.94 ± 0.048

2.126 ± 0.019

4.984 ± 0.024

2.849 ± 0.029

10.314 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.023

2.522 ± 0.027

5.214 ± 0.028

3.278 ± 0.021

6.971 ± 0.043

5.021 ± 0.031

5.287 ± 0.032

7.193 ± 0.032

1.428 ± 0.016

2.25 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski