Rhizobium sp. CF142

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J1T8W9|J1T8W9_9RHIZ ABC-type proline/glycine betaine transport system permease component OS=Rhizobium sp. CF142 OX=1144314 GN=PMI11_03582 PE=3 SV=1
MM1 pKa = 7.71PFVEE5 pKa = 4.45SQVNTFTVNSQYY17 pKa = 10.44STVTTQLPGGGWVITWASTLEE38 pKa = 4.54DD39 pKa = 3.74GSGTGVYY46 pKa = 9.35QQVFDD51 pKa = 4.02ATGSPSGPEE60 pKa = 3.71TRR62 pKa = 11.84VNTIVEE68 pKa = 4.76GNQEE72 pKa = 4.05MPAIAPLADD81 pKa = 4.17GGWVITWGNQGISTPGVYY99 pKa = 9.29QQAFNADD106 pKa = 3.43GTPRR110 pKa = 11.84GSEE113 pKa = 4.17TQVNTYY119 pKa = 9.88EE120 pKa = 4.39GAGQGSRR127 pKa = 11.84SVMGLSDD134 pKa = 4.1GGWVVSWLSVGQDD147 pKa = 3.02GSGYY151 pKa = 10.7GIYY154 pKa = 9.75QQAFAADD161 pKa = 3.89GSKK164 pKa = 10.07IAGEE168 pKa = 4.31TRR170 pKa = 11.84VNTHH174 pKa = 5.53TDD176 pKa = 3.06GNQWLGSMAPLDD188 pKa = 3.94GGGWVATWSSPGQDD202 pKa = 3.14GSEE205 pKa = 3.78TGVFQQIYY213 pKa = 8.93NADD216 pKa = 3.81GTPHH220 pKa = 6.8GGEE223 pKa = 4.24TLVNTYY229 pKa = 9.46TNSYY233 pKa = 9.01QDD235 pKa = 3.47SPSVVARR242 pKa = 11.84NGGWVVIWEE251 pKa = 4.36SLGQDD256 pKa = 3.18GSSRR260 pKa = 11.84GIYY263 pKa = 8.12QQAYY267 pKa = 8.29NADD270 pKa = 3.54GTRR273 pKa = 11.84FGTEE277 pKa = 3.66TRR279 pKa = 11.84VNTATDD285 pKa = 3.42GNEE288 pKa = 4.05AYY290 pKa = 9.93HH291 pKa = 7.11ASAALDD297 pKa = 3.82DD298 pKa = 4.97GGWVVIWISGDD309 pKa = 3.0AHH311 pKa = 7.57LMQQAYY317 pKa = 9.16NANGTPQGGEE327 pKa = 4.14TQVDD331 pKa = 3.4TDD333 pKa = 3.3GTYY336 pKa = 10.54PGNQKK341 pKa = 8.06VTALSDD347 pKa = 3.68GGWVVTWMVIGSSEE361 pKa = 4.21TFYY364 pKa = 11.07HH365 pKa = 7.19VYY367 pKa = 9.69QQAFNADD374 pKa = 3.27GTKK377 pKa = 10.73NGDD380 pKa = 3.27EE381 pKa = 4.11ALINTLTHH389 pKa = 6.31TYY391 pKa = 10.94KK392 pKa = 10.89DD393 pKa = 3.62IPQVAALDD401 pKa = 4.03DD402 pKa = 4.71GGWVVSWASDD412 pKa = 2.99SDD414 pKa = 3.94YY415 pKa = 11.33TDD417 pKa = 3.12VQGYY421 pKa = 10.22DD422 pKa = 2.85SGIFQVRR429 pKa = 11.84FDD431 pKa = 3.97TDD433 pKa = 2.64AHH435 pKa = 5.95AVEE438 pKa = 4.94LPSNRR443 pKa = 11.84IYY445 pKa = 10.35GTYY448 pKa = 9.23PDD450 pKa = 4.34KK451 pKa = 11.44NLVGTAGDD459 pKa = 3.74DD460 pKa = 3.88AIYY463 pKa = 10.55ASSGNDD469 pKa = 3.19TLTGLAGNDD478 pKa = 4.04HH479 pKa = 7.23LDD481 pKa = 3.11GGYY484 pKa = 10.66GHH486 pKa = 7.4DD487 pKa = 4.52RR488 pKa = 11.84MTGGLGDD495 pKa = 3.69DD496 pKa = 4.09TYY498 pKa = 11.31IVDD501 pKa = 4.07TSADD505 pKa = 3.56RR506 pKa = 11.84VVEE509 pKa = 3.88LAGQGTDD516 pKa = 3.37TILVQVSYY524 pKa = 11.45SLGANVEE531 pKa = 4.01NLAFVAEE538 pKa = 4.46GNLYY542 pKa = 10.92ASGNALANVITGNSGNNTLGGAAGSDD568 pKa = 3.66TLSGLDD574 pKa = 3.65GNDD577 pKa = 3.61YY578 pKa = 11.24LDD580 pKa = 4.83GGADD584 pKa = 3.48NDD586 pKa = 3.93KK587 pKa = 11.09LYY589 pKa = 11.09GGSGDD594 pKa = 5.24DD595 pKa = 3.62ILAGGTGDD603 pKa = 3.67DD604 pKa = 4.27TMDD607 pKa = 4.51GGDD610 pKa = 4.04GNDD613 pKa = 3.26KK614 pKa = 11.14YY615 pKa = 11.25KK616 pKa = 10.74VDD618 pKa = 3.66SANDD622 pKa = 3.63VVHH625 pKa = 6.79DD626 pKa = 4.2TGDD629 pKa = 5.18GIWDD633 pKa = 3.73WNDD636 pKa = 3.08IIYY639 pKa = 8.55STAEE643 pKa = 4.16TYY645 pKa = 11.12SLAGTGAEE653 pKa = 4.15TLILQAGAVTGIGDD667 pKa = 3.72SGNNQISGNSAANTLVSGGGSDD689 pKa = 3.72GLAGGGGVDD698 pKa = 3.74TFVLAAPSASNFAFINDD715 pKa = 4.02FGADD719 pKa = 3.64DD720 pKa = 4.34FLAFQSSDD728 pKa = 3.56FQGMTTATLDD738 pKa = 3.45FHH740 pKa = 7.08VGKK743 pKa = 8.3TAIGADD749 pKa = 2.99AQFYY753 pKa = 10.67FNTSDD758 pKa = 3.33RR759 pKa = 11.84TLYY762 pKa = 10.16WDD764 pKa = 4.57DD765 pKa = 5.13DD766 pKa = 4.03GTGGDD771 pKa = 3.63GAVRR775 pKa = 11.84VATLNGAYY783 pKa = 9.82VLQSGDD789 pKa = 3.44FLFAA793 pKa = 5.24

Molecular weight: 82.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J2AQL1|J2AQL1_9RHIZ Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme OS=Rhizobium sp. CF142 OX=1144314 GN=glnE PE=3 SV=1
MM1 pKa = 7.56ANLVNEE7 pKa = 4.44LVSGVVNSVLKK18 pKa = 10.69EE19 pKa = 3.87ILKK22 pKa = 9.04KK23 pKa = 7.5TTGRR27 pKa = 11.84TTTKK31 pKa = 9.97RR32 pKa = 11.84KK33 pKa = 9.1RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.0AASATTSRR44 pKa = 11.84TTRR47 pKa = 11.84KK48 pKa = 8.21ATPTSSKK55 pKa = 9.87PARR58 pKa = 11.84KK59 pKa = 8.82QVSKK63 pKa = 10.81RR64 pKa = 11.84RR65 pKa = 11.84TAAGRR70 pKa = 11.84SRR72 pKa = 11.84QRR74 pKa = 11.84RR75 pKa = 11.84AA76 pKa = 2.78

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7229

0

7229

2195810

21

4433

303.8

33.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.74 ± 0.034

0.789 ± 0.01

5.679 ± 0.026

5.706 ± 0.029

3.996 ± 0.019

8.234 ± 0.031

2.027 ± 0.015

5.895 ± 0.02

3.707 ± 0.02

10.013 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.564 ± 0.014

2.911 ± 0.018

4.883 ± 0.022

3.142 ± 0.017

6.512 ± 0.031

5.907 ± 0.025

5.337 ± 0.023

7.269 ± 0.023

1.313 ± 0.011

2.375 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski