Amino acid dipepetide frequency for Clostridium sp. CAG:167

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.561AlaAla: 6.561 ± 0.134	1.051AlaCys: 1.051 ± 0.043	4.202AlaAsp: 4.202 ± 0.085	4.741AlaGlu: 4.741 ± 0.092	2.817AlaPhe: 2.817 ± 0.072	6.197AlaGly: 6.197 ± 0.112	1.11AlaHis: 1.11 ± 0.04	4.312AlaIle: 4.312 ± 0.095	5.442AlaLys: 5.442 ± 0.111	6.588AlaLeu: 6.588 ± 0.121	2.49AlaMet: 2.49 ± 0.066	2.378AlaAsn: 2.378 ± 0.073	2.01AlaPro: 2.01 ± 0.061	2.156AlaGln: 2.156 ± 0.062	3.021AlaArg: 3.021 ± 0.076	4.337AlaSer: 4.337 ± 0.089	3.521AlaThr: 3.521 ± 0.086	6.306AlaVal: 6.306 ± 0.104	0.606AlaTrp: 0.606 ± 0.03	2.769AlaTyr: 2.769 ± 0.064	0.003AlaXaa: 0.003 ± 0.002
Cys	0.877CysAla: 0.877 ± 0.037	0.301CysCys: 0.301 ± 0.022	0.786CysAsp: 0.786 ± 0.037	0.842CysGlu: 0.842 ± 0.035	0.682CysPhe: 0.682 ± 0.033	1.436CysGly: 1.436 ± 0.048	0.373CysHis: 0.373 ± 0.028	1.086CysIle: 1.086 ± 0.047	0.848CysLys: 0.848 ± 0.041	1.289CysLeu: 1.289 ± 0.048	0.477CysMet: 0.477 ± 0.028	0.517CysAsn: 0.517 ± 0.031	0.575CysPro: 0.575 ± 0.036	0.575CysGln: 0.575 ± 0.032	0.799CysArg: 0.799 ± 0.039	0.875CysSer: 0.875 ± 0.041	0.69CysThr: 0.69 ± 0.034	1.081CysVal: 1.081 ± 0.044	0.079CysTrp: 0.079 ± 0.011	0.588CysTyr: 0.588 ± 0.034	0.0CysXaa: 0.0 ± 0.0
Asp	4.012AspAla: 4.012 ± 0.075	0.832AspCys: 0.832 ± 0.039	2.819AspAsp: 2.819 ± 0.078	4.581AspGlu: 4.581 ± 0.1	2.454AspPhe: 2.454 ± 0.065	4.277AspGly: 4.277 ± 0.096	0.999AspHis: 0.999 ± 0.039	4.126AspIle: 4.126 ± 0.091	3.973AspLys: 3.973 ± 0.095	4.81AspLeu: 4.81 ± 0.102	1.894AspMet: 1.894 ± 0.059	2.113AspAsn: 2.113 ± 0.057	1.794AspPro: 1.794 ± 0.059	1.76AspGln: 1.76 ± 0.052	2.326AspArg: 2.326 ± 0.059	3.251AspSer: 3.251 ± 0.082	3.105AspThr: 3.105 ± 0.071	3.82AspVal: 3.82 ± 0.085	0.563AspTrp: 0.563 ± 0.03	2.847AspTyr: 2.847 ± 0.072	0.002AspXaa: 0.002 ± 0.002
Glu	5.344GluAla: 5.344 ± 0.107	0.789GluCys: 0.789 ± 0.036	4.461GluAsp: 4.461 ± 0.086	7.297GluGlu: 7.297 ± 0.151	2.516GluPhe: 2.516 ± 0.071	4.512GluGly: 4.512 ± 0.09	1.256GluHis: 1.256 ± 0.056	5.263GluIle: 5.263 ± 0.101	7.509GluLys: 7.509 ± 0.139	6.122GluLeu: 6.122 ± 0.127	2.43GluMet: 2.43 ± 0.067	3.633GluAsn: 3.633 ± 0.076	1.989GluPro: 1.989 ± 0.062	3.033GluGln: 3.033 ± 0.074	3.015GluArg: 3.015 ± 0.084	3.313GluSer: 3.313 ± 0.074	3.95GluThr: 3.95 ± 0.087	4.806GluVal: 4.806 ± 0.096	0.593GluTrp: 0.593 ± 0.031	2.987GluTyr: 2.987 ± 0.074	0.002GluXaa: 0.002 ± 0.002
Phe	2.834PheAla: 2.834 ± 0.066	0.777PheCys: 0.777 ± 0.037	2.402PheAsp: 2.402 ± 0.066	2.638PheGlu: 2.638 ± 0.076	1.741PhePhe: 1.741 ± 0.066	2.863PheGly: 2.863 ± 0.068	0.872PheHis: 0.872 ± 0.038	2.365PheIle: 2.365 ± 0.078	1.97PheLys: 1.97 ± 0.059	3.424PheLeu: 3.424 ± 0.1	1.282PheMet: 1.282 ± 0.049	1.406PheAsn: 1.406 ± 0.051	1.267PhePro: 1.267 ± 0.046	1.246PheGln: 1.246 ± 0.046	1.622PheArg: 1.622 ± 0.057	2.79PheSer: 2.79 ± 0.083	2.207PheThr: 2.207 ± 0.057	3.004PheVal: 3.004 ± 0.068	0.396PheTrp: 0.396 ± 0.026	1.679PheTyr: 1.679 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	4.905GlyAla: 4.905 ± 0.099	1.206GlyCys: 1.206 ± 0.048	3.533GlyAsp: 3.533 ± 0.076	4.895GlyGlu: 4.895 ± 0.099	2.922GlyPhe: 2.922 ± 0.066	4.838GlyGly: 4.838 ± 0.136	1.276GlyHis: 1.276 ± 0.05	5.897GlyIle: 5.897 ± 0.116	6.368GlyLys: 6.368 ± 0.117	5.609GlyLeu: 5.609 ± 0.12	2.416GlyMet: 2.416 ± 0.062	2.956GlyAsn: 2.956 ± 0.077	1.366GlyPro: 1.366 ± 0.048	2.375GlyGln: 2.375 ± 0.064	3.164GlyArg: 3.164 ± 0.077	4.41GlySer: 4.41 ± 0.091	4.474GlyThr: 4.474 ± 0.083	5.697GlyVal: 5.697 ± 0.113	0.625GlyTrp: 0.625 ± 0.035	3.158GlyTyr: 3.158 ± 0.083	0.003GlyXaa: 0.003 ± 0.002
His	0.967HisAla: 0.967 ± 0.046	0.365HisCys: 0.365 ± 0.028	0.861HisAsp: 0.861 ± 0.038	1.053HisGlu: 1.053 ± 0.046	0.878HisPhe: 0.878 ± 0.036	1.425HisGly: 1.425 ± 0.052	0.425HisHis: 0.425 ± 0.03	1.378HisIle: 1.378 ± 0.053	1.021HisLys: 1.021 ± 0.04	1.485HisLeu: 1.485 ± 0.052	0.501HisMet: 0.501 ± 0.029	0.693HisAsn: 0.693 ± 0.034	0.869HisPro: 0.869 ± 0.039	0.579HisGln: 0.579 ± 0.035	0.84HisArg: 0.84 ± 0.042	1.018HisSer: 1.018 ± 0.043	1.002HisThr: 1.002 ± 0.046	1.078HisVal: 1.078 ± 0.04	0.136HisTrp: 0.136 ± 0.014	0.751HisTyr: 0.751 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.014IleAla: 5.014 ± 0.113	1.262IleCys: 1.262 ± 0.045	3.638IleAsp: 3.638 ± 0.089	4.377IleGlu: 4.377 ± 0.093	2.576IlePhe: 2.576 ± 0.062	4.665IleGly: 4.665 ± 0.097	1.517IleHis: 1.517 ± 0.059	4.058IleIle: 4.058 ± 0.107	4.226IleLys: 4.226 ± 0.095	6.937IleLeu: 6.937 ± 0.132	1.899IleMet: 1.899 ± 0.06	2.559IleAsn: 2.559 ± 0.071	2.92IlePro: 2.92 ± 0.075	2.752IleGln: 2.752 ± 0.065	3.632IleArg: 3.632 ± 0.082	4.369IleSer: 4.369 ± 0.089	4.155IleThr: 4.155 ± 0.089	4.952IleVal: 4.952 ± 0.107	0.556IleTrp: 0.556 ± 0.033	2.622IleTyr: 2.622 ± 0.063	0.002IleXaa: 0.002 ± 0.001
Lys	5.366LysAla: 5.366 ± 0.107	0.724LysCys: 0.724 ± 0.039	4.672LysAsp: 4.672 ± 0.091	7.612LysGlu: 7.612 ± 0.146	1.905LysPhe: 1.905 ± 0.061	4.971LysGly: 4.971 ± 0.106	0.904LysHis: 0.904 ± 0.047	5.285LysIle: 5.285 ± 0.097	9.056LysLys: 9.056 ± 0.187	5.46LysLeu: 5.46 ± 0.097	2.581LysMet: 2.581 ± 0.071	3.923LysAsn: 3.923 ± 0.085	2.04LysPro: 2.04 ± 0.059	2.582LysGln: 2.582 ± 0.073	3.281LysArg: 3.281 ± 0.085	3.54LysSer: 3.54 ± 0.085	4.355LysThr: 4.355 ± 0.098	5.383LysVal: 5.383 ± 0.095	0.647LysTrp: 0.647 ± 0.036	2.998LysTyr: 2.998 ± 0.08	0.0LysXaa: 0.0 ± 0.0
Leu	6.763LeuAla: 6.763 ± 0.117	1.536LeuCys: 1.536 ± 0.062	4.922LeuAsp: 4.922 ± 0.101	6.0LeuGlu: 6.0 ± 0.129	3.644LeuPhe: 3.644 ± 0.108	5.94LeuGly: 5.94 ± 0.105	1.536LeuHis: 1.536 ± 0.052	5.272LeuIle: 5.272 ± 0.126	6.078LeuLys: 6.078 ± 0.104	7.628LeuLeu: 7.628 ± 0.165	2.557LeuMet: 2.557 ± 0.071	3.342LeuAsn: 3.342 ± 0.076	3.239LeuPro: 3.239 ± 0.064	3.012LeuGln: 3.012 ± 0.085	3.637LeuArg: 3.637 ± 0.084	5.975LeuSer: 5.975 ± 0.115	5.03LeuThr: 5.03 ± 0.088	5.788LeuVal: 5.788 ± 0.1	0.758LeuTrp: 0.758 ± 0.034	3.359LeuTyr: 3.359 ± 0.083	0.0LeuXaa: 0.0 ± 0.0
Met	2.619MetAla: 2.619 ± 0.068	0.369MetCys: 0.369 ± 0.026	2.067MetAsp: 2.067 ± 0.068	2.739MetGlu: 2.739 ± 0.065	0.999MetPhe: 0.999 ± 0.046	2.173MetGly: 2.173 ± 0.064	0.452MetHis: 0.452 ± 0.025	2.21MetIle: 2.21 ± 0.061	2.736MetLys: 2.736 ± 0.073	2.554MetLeu: 2.554 ± 0.056	0.918MetMet: 0.918 ± 0.042	1.604MetAsn: 1.604 ± 0.05	1.099MetPro: 1.099 ± 0.036	1.091MetGln: 1.091 ± 0.038	1.259MetArg: 1.259 ± 0.045	1.787MetSer: 1.787 ± 0.052	1.826MetThr: 1.826 ± 0.058	2.089MetVal: 2.089 ± 0.07	0.163MetTrp: 0.163 ± 0.016	0.973MetTyr: 0.973 ± 0.04	0.0MetXaa: 0.0 ± 0.0
Asn	2.79AsnAla: 2.79 ± 0.075	0.604AsnCys: 0.604 ± 0.03	1.95AsnAsp: 1.95 ± 0.057	2.628AsnGlu: 2.628 ± 0.063	1.511AsnPhe: 1.511 ± 0.047	3.335AsnGly: 3.335 ± 0.088	0.767AsnHis: 0.767 ± 0.038	3.066AsnIle: 3.066 ± 0.072	2.949AsnLys: 2.949 ± 0.07	3.472AsnLeu: 3.472 ± 0.076	1.297AsnMet: 1.297 ± 0.048	1.787AsnAsn: 1.787 ± 0.067	1.747AsnPro: 1.747 ± 0.056	1.482AsnGln: 1.482 ± 0.047	1.872AsnArg: 1.872 ± 0.059	2.2AsnSer: 2.2 ± 0.057	2.281AsnThr: 2.281 ± 0.067	2.744AsnVal: 2.744 ± 0.084	0.38AsnTrp: 0.38 ± 0.025	1.668AsnTyr: 1.668 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	2.481ProAla: 2.481 ± 0.069	0.431ProCys: 0.431 ± 0.028	2.245ProAsp: 2.245 ± 0.059	3.245ProGlu: 3.245 ± 0.087	1.431ProPhe: 1.431 ± 0.045	2.456ProGly: 2.456 ± 0.07	0.534ProHis: 0.534 ± 0.03	1.771ProIle: 1.771 ± 0.059	1.858ProLys: 1.858 ± 0.062	2.573ProLeu: 2.573 ± 0.073	0.85ProMet: 0.85 ± 0.037	1.018ProAsn: 1.018 ± 0.043	0.72ProPro: 0.72 ± 0.039	1.034ProGln: 1.034 ± 0.039	0.969ProArg: 0.969 ± 0.043	1.89ProSer: 1.89 ± 0.062	1.607ProThr: 1.607 ± 0.065	3.126ProVal: 3.126 ± 0.072	0.339ProTrp: 0.339 ± 0.024	1.43ProTyr: 1.43 ± 0.053	0.0ProXaa: 0.0 ± 0.0
Gln	2.649GlnAla: 2.649 ± 0.067	0.482GlnCys: 0.482 ± 0.03	1.828GlnAsp: 1.828 ± 0.054	2.731GlnGlu: 2.731 ± 0.075	1.206GlnPhe: 1.206 ± 0.042	2.584GlnGly: 2.584 ± 0.063	0.449GlnHis: 0.449 ± 0.029	2.75GlnIle: 2.75 ± 0.067	2.895GlnLys: 2.895 ± 0.08	2.605GlnLeu: 2.605 ± 0.075	1.37GlnMet: 1.37 ± 0.044	1.474GlnAsn: 1.474 ± 0.05	0.926GlnPro: 0.926 ± 0.041	1.333GlnGln: 1.333 ± 0.047	1.458GlnArg: 1.458 ± 0.062	1.801GlnSer: 1.801 ± 0.051	1.794GlnThr: 1.794 ± 0.055	2.571GlnVal: 2.571 ± 0.063	0.425GlnTrp: 0.425 ± 0.029	1.458GlnTyr: 1.458 ± 0.049	0.0GlnXaa: 0.0 ± 0.0
Arg	2.333ArgAla: 2.333 ± 0.063	0.615ArgCys: 0.615 ± 0.034	2.115ArgAsp: 2.115 ± 0.062	3.63ArgGlu: 3.63 ± 0.09	1.79ArgPhe: 1.79 ± 0.055	2.579ArgGly: 2.579 ± 0.076	0.808ArgHis: 0.808 ± 0.04	3.397ArgIle: 3.397 ± 0.069	3.43ArgLys: 3.43 ± 0.073	3.797ArgLeu: 3.797 ± 0.09	1.531ArgMet: 1.531 ± 0.056	1.75ArgAsn: 1.75 ± 0.056	1.252ArgPro: 1.252 ± 0.048	1.978ArgGln: 1.978 ± 0.059	2.264ArgArg: 2.264 ± 0.073	2.316ArgSer: 2.316 ± 0.056	2.15ArgThr: 2.15 ± 0.062	2.807ArgVal: 2.807 ± 0.077	0.407ArgTrp: 0.407 ± 0.028	1.993ArgTyr: 1.993 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	3.982SerAla: 3.982 ± 0.084	0.75SerCys: 0.75 ± 0.035	3.329SerAsp: 3.329 ± 0.073	3.513SerGlu: 3.513 ± 0.071	2.552SerPhe: 2.552 ± 0.064	5.019SerGly: 5.019 ± 0.108	1.094SerHis: 1.094 ± 0.048	3.89SerIle: 3.89 ± 0.086	3.916SerLys: 3.916 ± 0.089	5.398SerLeu: 5.398 ± 0.108	1.945SerMet: 1.945 ± 0.054	2.172SerAsn: 2.172 ± 0.065	1.685SerPro: 1.685 ± 0.052	2.232SerGln: 2.232 ± 0.064	2.624SerArg: 2.624 ± 0.059	3.901SerSer: 3.901 ± 0.123	2.993SerThr: 2.993 ± 0.081	4.714SerVal: 4.714 ± 0.09	0.491SerTrp: 0.491 ± 0.035	2.635SerTyr: 2.635 ± 0.072	0.0SerXaa: 0.0 ± 0.0
Thr	4.675ThrAla: 4.675 ± 0.106	0.642ThrCys: 0.642 ± 0.032	3.161ThrAsp: 3.161 ± 0.076	3.659ThrGlu: 3.659 ± 0.1	1.989ThrPhe: 1.989 ± 0.057	4.724ThrGly: 4.724 ± 0.088	0.829ThrHis: 0.829 ± 0.035	3.977ThrIle: 3.977 ± 0.089	3.96ThrLys: 3.96 ± 0.094	4.935ThrLeu: 4.935 ± 0.096	1.539ThrMet: 1.539 ± 0.045	2.058ThrAsn: 2.058 ± 0.065	2.265ThrPro: 2.265 ± 0.068	1.547ThrGln: 1.547 ± 0.054	1.978ThrArg: 1.978 ± 0.052	3.156ThrSer: 3.156 ± 0.077	3.264ThrThr: 3.264 ± 0.113	5.041ThrVal: 5.041 ± 0.112	0.51ThrTrp: 0.51 ± 0.034	2.186ThrTyr: 2.186 ± 0.069	0.0ThrXaa: 0.0 ± 0.0
Val	5.393ValAla: 5.393 ± 0.122	1.297ValCys: 1.297 ± 0.05	4.174ValAsp: 4.174 ± 0.078	4.909ValGlu: 4.909 ± 0.097	3.044ValPhe: 3.044 ± 0.084	4.325ValGly: 4.325 ± 0.071	1.091ValHis: 1.091 ± 0.046	5.179ValIle: 5.179 ± 0.104	5.46ValLys: 5.46 ± 0.126	6.973ValLeu: 6.973 ± 0.135	2.207ValMet: 2.207 ± 0.051	2.912ValAsn: 2.912 ± 0.08	2.723ValPro: 2.723 ± 0.065	2.127ValGln: 2.127 ± 0.061	2.915ValArg: 2.915 ± 0.079	4.962ValSer: 4.962 ± 0.11	4.941ValThr: 4.941 ± 0.121	5.74ValVal: 5.74 ± 0.102	0.717ValTrp: 0.717 ± 0.04	2.963ValTyr: 2.963 ± 0.073	0.003ValXaa: 0.003 ± 0.002
Trp	0.458TrpAla: 0.458 ± 0.03	0.132TrpCys: 0.132 ± 0.016	0.564TrpAsp: 0.564 ± 0.027	0.617TrpGlu: 0.617 ± 0.032	0.384TrpPhe: 0.384 ± 0.025	0.625TrpGly: 0.625 ± 0.042	0.203TrpHis: 0.203 ± 0.02	0.717TrpIle: 0.717 ± 0.034	0.729TrpLys: 0.729 ± 0.035	0.81TrpLeu: 0.81 ± 0.041	0.325TrpMet: 0.325 ± 0.026	0.428TrpAsn: 0.428 ± 0.026	0.216TrpPro: 0.216 ± 0.018	0.469TrpGln: 0.469 ± 0.028	0.322TrpArg: 0.322 ± 0.024	0.46TrpSer: 0.46 ± 0.031	0.415TrpThr: 0.415 ± 0.031	0.503TrpVal: 0.503 ± 0.033	0.105TrpTrp: 0.105 ± 0.014	0.347TrpTyr: 0.347 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.595TyrAla: 2.595 ± 0.057	0.609TyrCys: 0.609 ± 0.032	2.63TyrAsp: 2.63 ± 0.057	3.112TyrGlu: 3.112 ± 0.074	1.744TyrPhe: 1.744 ± 0.063	3.15TyrGly: 3.15 ± 0.082	0.831TyrHis: 0.831 ± 0.039	2.682TyrIle: 2.682 ± 0.061	2.863TyrLys: 2.863 ± 0.074	3.548TyrLeu: 3.548 ± 0.077	1.214TyrMet: 1.214 ± 0.044	1.807TyrAsn: 1.807 ± 0.062	1.303TyrPro: 1.303 ± 0.048	1.427TyrGln: 1.427 ± 0.047	1.947TyrArg: 1.947 ± 0.06	2.482TyrSer: 2.482 ± 0.073	2.337TyrThr: 2.337 ± 0.068	2.801TyrVal: 2.801 ± 0.069	0.328TyrTrp: 0.328 ± 0.025	1.983TyrTyr: 1.983 ± 0.061	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.002XaaAla: 0.002 ± 0.001	0.002XaaCys: 0.002 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.006XaaIle: 0.006 ± 0.003	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.002XaaMet: 0.002 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.002	0.002XaaTrp: 0.002 ± 0.002	0.0XaaTyr: 0.0 ± 0.0	0.022XaaXaa: 0.022 ± 0.007

Statistics based on 1994 proteins (630825 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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