Nesterenkonia jeotgali
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2504 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W8ICD3|A0A0W8ICD3_9MICC Acyl-CoA_dh_2 domain-containing protein OS=Nesterenkonia jeotgali OX=317018 GN=AVL63_13120 PE=4 SV=1
MM1 pKa = 7.47 TGPSPRR7 pKa = 11.84 HH8 pKa = 5.43 LWSSSLALTMLLLVTSSCSAEE29 pKa = 3.73 QSAQNPSEE37 pKa = 4.23 QEE39 pKa = 3.62 PSAQDD44 pKa = 3.44 SDD46 pKa = 3.91 SSPPAEE52 pKa = 4.0 QRR54 pKa = 11.84 SSPSSAAVGAEE65 pKa = 3.93 DD66 pKa = 4.21 PEE68 pKa = 4.43 EE69 pKa = 3.86 EE70 pKa = 4.32 AEE72 pKa = 4.24 AVTEE76 pKa = 4.2 VEE78 pKa = 4.24 EE79 pKa = 4.32 EE80 pKa = 4.13 EE81 pKa = 4.05 PSIRR85 pKa = 11.84 DD86 pKa = 3.26 VEE88 pKa = 4.29 FADD91 pKa = 5.09 LEE93 pKa = 4.15 WTEE96 pKa = 4.03 WVNEE100 pKa = 4.15 VVLIPAEE107 pKa = 4.45 GSDD110 pKa = 3.7 GPEE113 pKa = 3.72 FEE115 pKa = 4.54 VSEE118 pKa = 4.61 EE119 pKa = 3.55 IHH121 pKa = 6.33 YY122 pKa = 11.24 ADD124 pKa = 4.93 ADD126 pKa = 4.07 GDD128 pKa = 4.0 GHH130 pKa = 6.77 EE131 pKa = 5.17 DD132 pKa = 3.49 ALVSLGWTDD141 pKa = 3.85 DD142 pKa = 4.04 RR143 pKa = 11.84 GFEE146 pKa = 4.6 DD147 pKa = 4.51 IWYY150 pKa = 8.61 IWAWDD155 pKa = 3.89 PDD157 pKa = 4.13 TEE159 pKa = 4.46 SAYY162 pKa = 10.32 QAGGPVARR170 pKa = 11.84 AANCGDD176 pKa = 3.35 HH177 pKa = 6.5 VEE179 pKa = 4.39 SVSAGEE185 pKa = 3.74 DD186 pKa = 3.06 HH187 pKa = 7.27 FVIEE191 pKa = 4.09 EE192 pKa = 4.16 HH193 pKa = 6.58 LWPQNADD200 pKa = 3.34 HH201 pKa = 7.63 DD202 pKa = 4.62 CASTPSIQVTRR213 pKa = 11.84 HH214 pKa = 5.65 LVLEE218 pKa = 4.67 DD219 pKa = 3.91 DD220 pKa = 4.31 WPVMISPVRR229 pKa = 11.84 AHH231 pKa = 7.11 GGMCPQILGTDD242 pKa = 3.86 DD243 pKa = 3.7 LWPVEE248 pKa = 4.2 DD249 pKa = 4.7 FEE251 pKa = 5.65 FRR253 pKa = 11.84 TSPEE257 pKa = 3.55 EE258 pKa = 3.72 AAGLVGDD265 pKa = 4.64 PAPTAFAEE273 pKa = 4.13 ADD275 pKa = 3.23 VWSRR279 pKa = 11.84 LWLHH283 pKa = 5.89 YY284 pKa = 9.35 PNWMLVHH291 pKa = 6.73 LAYY294 pKa = 10.09 MNPADD299 pKa = 4.62 GPPVLEE305 pKa = 5.31 GGYY308 pKa = 7.65 TPCGWVYY315 pKa = 10.96 LEE317 pKa = 4.49 EE318 pKa = 4.28 DD319 pKa = 3.97 TRR321 pKa = 11.84 PVPHH325 pKa = 7.35 DD326 pKa = 4.23 PYY328 pKa = 10.68 PPEE331 pKa = 4.28 DD332 pKa = 3.25
Molecular weight: 36.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.617
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.923
Patrickios 1.049
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.836
Protein with the highest isoelectric point:
>tr|A0A0W8II41|A0A0W8II41_9MICC Uncharacterized protein OS=Nesterenkonia jeotgali OX=317018 GN=AVL63_10775 PE=4 SV=1
MM1 pKa = 7.48 RR2 pKa = 11.84 AALFVLVFAVTVIGASLAGSQNLAFVLLMGAAVAALASTVMTASGRR48 pKa = 11.84 QALRR52 pKa = 11.84 RR53 pKa = 11.84 TRR55 pKa = 11.84 GNLTQGWSRR64 pKa = 11.84 RR65 pKa = 11.84 PGG67 pKa = 3.12
Molecular weight: 6.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2504
0
2504
820621
38
1656
327.7
35.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.617 ± 0.064
0.553 ± 0.012
5.623 ± 0.038
6.796 ± 0.054
3.036 ± 0.029
8.769 ± 0.043
2.267 ± 0.024
4.377 ± 0.031
2.204 ± 0.036
10.389 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.095 ± 0.022
2.067 ± 0.023
5.353 ± 0.035
3.649 ± 0.028
6.847 ± 0.044
6.15 ± 0.034
5.908 ± 0.029
7.935 ± 0.036
1.414 ± 0.019
1.95 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here