Tortispora caseinolytica NRRL Y-17796
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4657 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E4T9R0|A0A1E4T9R0_9ASCO Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 OX=767744 GN=CANCADRAFT_46371 PE=4 SV=1
MM1 pKa = 7.68 LSTVTLTEE9 pKa = 4.03 TSLCKK14 pKa = 10.25 PPTTTTTVYY23 pKa = 10.48 EE24 pKa = 4.26 KK25 pKa = 9.97 TYY27 pKa = 10.79 SSANGIVTAYY37 pKa = 10.62 DD38 pKa = 4.26 DD39 pKa = 3.94 YY40 pKa = 11.61 CLPEE44 pKa = 4.23 TKK46 pKa = 10.29 FVDD49 pKa = 3.45 NVYY52 pKa = 10.57 CYY54 pKa = 11.15 ACTPTVLTYY63 pKa = 10.52 VPKK66 pKa = 10.83 GPLVNDD72 pKa = 2.94 SHH74 pKa = 6.38 TYY76 pKa = 9.42 VWGLYY81 pKa = 8.05 QEE83 pKa = 4.68 ISFSVSIAFDD93 pKa = 3.15 ITIEE97 pKa = 4.12 KK98 pKa = 9.39 PIAVTKK104 pKa = 10.28 FEE106 pKa = 4.37 NADD109 pKa = 4.02 AIQTTLSSVALMPVIPLSSNQASISSEE136 pKa = 3.33 ISTSPSSILATTSTFSNISVGEE158 pKa = 3.86 GAIIGVASATAVEE171 pKa = 4.51 STTVQEE177 pKa = 4.66 RR178 pKa = 11.84 PTAYY182 pKa = 8.4 ITSNDD187 pKa = 3.13 IRR189 pKa = 11.84 NGDD192 pKa = 3.42 LTTIVEE198 pKa = 4.59 SFKK201 pKa = 11.24 EE202 pKa = 4.05 MAVSSSVEE210 pKa = 3.58 ISSFAQLSSSYY221 pKa = 11.04 AEE223 pKa = 4.14 SSPALTSLISSSVVQNDD240 pKa = 2.88 ISSSYY245 pKa = 10.29 PPTSIPKK252 pKa = 9.34 VDD254 pKa = 4.06 SSSMDD259 pKa = 3.35 AVIVAPEE266 pKa = 3.97 YY267 pKa = 11.26 SLDD270 pKa = 4.06 PGPDD274 pKa = 3.72 RR275 pKa = 11.84 IPQSEE280 pKa = 4.41 MVDD283 pKa = 4.23 DD284 pKa = 5.64 IIKK287 pKa = 11.03 SMIDD291 pKa = 3.17 GLIIATHH298 pKa = 6.37 LPVVSIDD305 pKa = 3.68 PGEE308 pKa = 4.76 HH309 pKa = 6.57 NDD311 pKa = 3.66 DD312 pKa = 3.91 TVSDD316 pKa = 4.07 GLVGDD321 pKa = 4.6 NEE323 pKa = 4.94 LDD325 pKa = 3.37 TGIVDD330 pKa = 3.96 SSEE333 pKa = 4.06 DD334 pKa = 3.94 EE335 pKa = 4.1 SWSDD339 pKa = 3.24 IVQDD343 pKa = 3.5 VDD345 pKa = 4.8 DD346 pKa = 4.94 EE347 pKa = 4.64 EE348 pKa = 5.32 SVIDD352 pKa = 5.54 LEE354 pKa = 5.03 NPDD357 pKa = 5.13 LLGDD361 pKa = 4.52 DD362 pKa = 4.81 YY363 pKa = 12.08 AEE365 pKa = 4.12 EE366 pKa = 4.07 WSDD369 pKa = 4.04 LNEE372 pKa = 5.17 DD373 pKa = 3.57 GSAVDD378 pKa = 4.87 GVVSTDD384 pKa = 3.81 DD385 pKa = 4.38 LVPDD389 pKa = 3.62 TTGIAEE395 pKa = 4.16 TDD397 pKa = 3.77 FGSEE401 pKa = 3.66 SGTNISGLVGEE412 pKa = 4.56 DD413 pKa = 3.42 MSWTVSRR420 pKa = 11.84 LEE422 pKa = 4.82 DD423 pKa = 3.51 YY424 pKa = 11.4 SGDD427 pKa = 3.76 TEE429 pKa = 4.36 VTIDD433 pKa = 4.29 KK434 pKa = 10.7 ADD436 pKa = 3.39 KK437 pKa = 10.77
Molecular weight: 46.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.528
IPC_protein 3.554
Toseland 3.325
ProMoST 3.719
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.49
Rodwell 3.376
Grimsley 3.236
Solomon 3.541
Lehninger 3.503
Nozaki 3.656
DTASelect 3.897
Thurlkill 3.376
EMBOSS 3.49
Sillero 3.681
Patrickios 0.795
IPC_peptide 3.541
IPC2_peptide 3.656
IPC2.peptide.svr19 3.67
Protein with the highest isoelectric point:
>tr|A0A1E4TJU4|A0A1E4TJU4_9ASCO Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 OX=767744 GN=CANCADRAFT_11734 PE=4 SV=1
MM1 pKa = 7.57 HH2 pKa = 7.03 NQRR5 pKa = 11.84 HH6 pKa = 4.78 QPSMLQLIMSQPSTFQRR23 pKa = 11.84 RR24 pKa = 11.84 LKK26 pKa = 10.93 SRR28 pKa = 11.84 GNTYY32 pKa = 10.22 QPSTLRR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 10.01 RR41 pKa = 11.84 KK42 pKa = 9.22 FGFLARR48 pKa = 11.84 LRR50 pKa = 11.84 SRR52 pKa = 11.84 TGKK55 pKa = 10.47 KK56 pKa = 9.17 ILARR60 pKa = 11.84 RR61 pKa = 11.84 KK62 pKa = 10.12 LKK64 pKa = 10.49 GRR66 pKa = 11.84 WRR68 pKa = 11.84 LTHH71 pKa = 6.58
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.477
IPC2_protein 11.228
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.486
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.29
Sillero 12.793
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4656
1
4657
2057799
50
4853
441.9
49.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.834 ± 0.03
1.312 ± 0.015
5.944 ± 0.027
6.125 ± 0.032
4.032 ± 0.026
5.532 ± 0.036
2.234 ± 0.017
6.048 ± 0.025
5.461 ± 0.03
9.533 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.149 ± 0.012
4.199 ± 0.017
5.016 ± 0.03
3.643 ± 0.025
5.218 ± 0.024
9.164 ± 0.062
5.667 ± 0.021
6.361 ± 0.023
1.128 ± 0.012
3.399 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here