Apteryx rowi circovirus-like virus
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6TGK2|A0A0F6TGK2_9CIRC ATP-dependent helicase Rep OS=Apteryx rowi circovirus-like virus OX=1634476 PE=3 SV=1
MM1 pKa = 7.4 SKK3 pKa = 10.33 ARR5 pKa = 11.84 HH6 pKa = 4.78 WCFTLNNPDD15 pKa = 4.87 DD16 pKa = 4.65 EE17 pKa = 5.95 PDD19 pKa = 3.6 LSDD22 pKa = 4.08 TNIQYY27 pKa = 10.78 AIYY30 pKa = 9.98 QMEE33 pKa = 4.65 IAPHH37 pKa = 6.31 TDD39 pKa = 2.92 TPHH42 pKa = 5.22 FQGYY46 pKa = 10.01 IILFKK51 pKa = 10.35 KK52 pKa = 9.91 QRR54 pKa = 11.84 LAWVRR59 pKa = 11.84 AVLPRR64 pKa = 11.84 AHH66 pKa = 6.74 WEE68 pKa = 3.87 IARR71 pKa = 11.84 GTPSQNRR78 pKa = 11.84 DD79 pKa = 3.19 YY80 pKa = 10.25 CTKK83 pKa = 8.72 PDD85 pKa = 3.52 TRR87 pKa = 11.84 IGEE90 pKa = 4.1 PSEE93 pKa = 5.3 LGLFPEE99 pKa = 5.31 DD100 pKa = 3.48 AGQGSRR106 pKa = 11.84 TDD108 pKa = 3.38 LQAVHH113 pKa = 6.93 FALQHH118 pKa = 5.55 GLTNKK123 pKa = 9.89 EE124 pKa = 3.61 YY125 pKa = 9.9 ANQYY129 pKa = 7.72 FSTWIRR135 pKa = 11.84 YY136 pKa = 7.57 PNLIDD141 pKa = 4.18 TFAQSNIVPRR151 pKa = 11.84 TGDD154 pKa = 3.49 EE155 pKa = 4.26 EE156 pKa = 4.45 IEE158 pKa = 4.65 CILIIGPPGTGKK170 pKa = 10.02 SRR172 pKa = 11.84 YY173 pKa = 9.05 AEE175 pKa = 3.85 RR176 pKa = 11.84 LARR179 pKa = 11.84 SYY181 pKa = 11.55 NVGEE185 pKa = 4.42 FFRR188 pKa = 11.84 KK189 pKa = 9.28 QRR191 pKa = 11.84 GKK193 pKa = 9.83 WYY195 pKa = 10.0 DD196 pKa = 3.13 GYY198 pKa = 11.04 LGEE201 pKa = 5.02 RR202 pKa = 11.84 VIIFDD207 pKa = 4.08 DD208 pKa = 4.06 FRR210 pKa = 11.84 GSSLSFTDD218 pKa = 5.04 FKK220 pKa = 11.59 LLVDD224 pKa = 4.69 RR225 pKa = 11.84 YY226 pKa = 9.8 ALRR229 pKa = 11.84 VEE231 pKa = 4.33 VKK233 pKa = 10.24 GRR235 pKa = 11.84 YY236 pKa = 8.75 VNMAATKK243 pKa = 9.74 FIITTNLSPDD253 pKa = 3.23 AWWGEE258 pKa = 4.24 EE259 pKa = 3.88 VTGPEE264 pKa = 3.98 FPAISRR270 pKa = 11.84 RR271 pKa = 11.84 ITEE274 pKa = 3.81 VLFFPEE280 pKa = 4.67 LNSFCRR286 pKa = 11.84 FDD288 pKa = 3.52 SYY290 pKa = 11.29 EE291 pKa = 4.01 AYY293 pKa = 10.68 LEE295 pKa = 3.93 YY296 pKa = 10.74 LRR298 pKa = 11.84 PQLILPHH305 pKa = 6.55 GLLPQAQAQYY315 pKa = 11.25 AFQEE319 pKa = 4.39 VIYY322 pKa = 10.8
Molecular weight: 37.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.564
IPC2_protein 5.626
IPC_protein 5.639
Toseland 5.957
ProMoST 5.931
Dawson 5.83
Bjellqvist 5.868
Wikipedia 5.804
Rodwell 5.804
Grimsley 6.084
Solomon 5.83
Lehninger 5.817
Nozaki 6.071
DTASelect 6.249
Thurlkill 6.211
EMBOSS 6.173
Sillero 6.16
Patrickios 3.91
IPC_peptide 5.842
IPC2_peptide 6.16
IPC2.peptide.svr19 6.145
Protein with the highest isoelectric point:
>tr|A0A0F6TGK2|A0A0F6TGK2_9CIRC ATP-dependent helicase Rep OS=Apteryx rowi circovirus-like virus OX=1634476 PE=3 SV=1
MM1 pKa = 7.05 VSYY4 pKa = 10.52 RR5 pKa = 11.84 KK6 pKa = 9.51 RR7 pKa = 11.84 KK8 pKa = 9.55 RR9 pKa = 11.84 SMRR12 pKa = 11.84 SKK14 pKa = 10.99 KK15 pKa = 10.52 SFTKK19 pKa = 9.67 IKK21 pKa = 10.21 RR22 pKa = 11.84 RR23 pKa = 11.84 PRR25 pKa = 11.84 VLNKK29 pKa = 9.72 FKK31 pKa = 10.77 RR32 pKa = 11.84 KK33 pKa = 9.19 NRR35 pKa = 11.84 GSQRR39 pKa = 11.84 IKK41 pKa = 8.39 RR42 pKa = 11.84 TRR44 pKa = 11.84 WPVDD48 pKa = 3.11 NPFGDD53 pKa = 3.43 NLFYY57 pKa = 10.77 KK58 pKa = 10.63 VRR60 pKa = 11.84 FSYY63 pKa = 8.93 GTTLTIPTTASSINLGFQMNSLASIISSAGNAPGLNSLGNAFVNYY108 pKa = 9.96 RR109 pKa = 11.84 IRR111 pKa = 11.84 GLKK114 pKa = 9.12 TKK116 pKa = 7.79 MTLWPDD122 pKa = 3.18 SATVPIVAYY131 pKa = 9.48 HH132 pKa = 6.73 LAGASQSEE140 pKa = 4.59 MASITPNVSTLPEE153 pKa = 4.26 LRR155 pKa = 11.84 WQKK158 pKa = 10.85 YY159 pKa = 5.69 RR160 pKa = 11.84 TCNYY164 pKa = 9.15 AAAGAKK170 pKa = 7.37 PTRR173 pKa = 11.84 LNSYY177 pKa = 9.44 FSVDD181 pKa = 3.21 KK182 pKa = 11.18 VYY184 pKa = 11.13 GPDD187 pKa = 3.13 AVVKK191 pKa = 10.58 NDD193 pKa = 3.31 SNFVGNITTAAPPAPPVFGAPGLGPGFRR221 pKa = 11.84 FGLFTMSGLNPTVAVNCVTKK241 pKa = 10.4 IEE243 pKa = 4.06 FTAYY247 pKa = 10.76 VNFFSKK253 pKa = 10.5 RR254 pKa = 11.84 GIINN258 pKa = 3.34
Molecular weight: 28.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 10.175
IPC_protein 10.979
Toseland 11.067
ProMoST 10.847
Dawson 11.14
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.403
Grimsley 11.184
Solomon 11.301
Lehninger 11.257
Nozaki 11.038
DTASelect 10.877
Thurlkill 11.052
EMBOSS 11.477
Sillero 11.067
Patrickios 11.111
IPC_peptide 11.316
IPC2_peptide 9.882
IPC2.peptide.svr19 8.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
580
258
322
290.0
32.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.586 ± 0.347
1.034 ± 0.163
4.138 ± 1.137
4.31 ± 1.974
6.207 ± 0.003
6.724 ± 0.402
1.379 ± 0.622
6.034 ± 0.624
5.172 ± 1.375
7.414 ± 0.76
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.552 ± 0.485
5.517 ± 1.402
6.552 ± 0.267
3.448 ± 1.19
7.586 ± 0.104
6.724 ± 1.374
6.724 ± 0.645
5.345 ± 1.023
1.724 ± 0.352
4.828 ± 0.597
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here