Geobacter sp. DSM 9736
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3513 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A212PIF8|A0A212PIF8_9DELT Quinol:cytochrome c oxidoreductase pentaheme cytochrome subunit OS=Geobacter sp. DSM 9736 OX=1277350 GN=SAMN06269301_2157 PE=4 SV=1
MM1 pKa = 7.78 GILVCYY7 pKa = 10.54 DD8 pKa = 3.41 DD9 pKa = 3.57 FTYY12 pKa = 11.0 DD13 pKa = 3.18 VVADD17 pKa = 4.23 FTFDD21 pKa = 3.51 TEE23 pKa = 4.37 DD24 pKa = 3.54 GRR26 pKa = 11.84 IAGYY30 pKa = 10.69 DD31 pKa = 3.56 NSCDD35 pKa = 3.26 WEE37 pKa = 4.35 KK38 pKa = 11.36 VVMEE42 pKa = 4.57 EE43 pKa = 5.29 FIPLPPLNVQTVLKK57 pKa = 10.38 KK58 pKa = 10.73
Molecular weight: 6.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A212PK61|A0A212PK61_9DELT Beta sliding clamp OS=Geobacter sp. DSM 9736 OX=1277350 GN=SAMN06269301_2761 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNVSRR12 pKa = 11.84 KK13 pKa = 7.52 RR14 pKa = 11.84 THH16 pKa = 6.03 GFLVRR21 pKa = 11.84 MSTKK25 pKa = 10.31 NGRR28 pKa = 11.84 LVIKK32 pKa = 10.39 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.58 RR41 pKa = 11.84 LAVTIASKK49 pKa = 11.0
Molecular weight: 5.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3513
0
3513
1189170
29
4151
338.5
37.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.339 ± 0.049
1.316 ± 0.03
5.122 ± 0.029
6.689 ± 0.067
4.016 ± 0.03
8.24 ± 0.052
2.095 ± 0.03
5.861 ± 0.033
4.717 ± 0.044
9.946 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.432 ± 0.022
3.292 ± 0.04
4.631 ± 0.03
3.036 ± 0.025
6.476 ± 0.048
6.101 ± 0.048
5.444 ± 0.061
7.385 ± 0.035
1.051 ± 0.019
2.811 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here