McMurdo Ice Shelf pond-associated circular DNA virus-1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075LZD6|A0A075LZD6_9VIRU Uncharacterized protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-1 OX=1521385 PE=4 SV=1
MM1 pKa = 7.27SQGKK5 pKa = 8.11HH6 pKa = 4.22WQFTLNNPTQDD17 pKa = 3.35EE18 pKa = 4.64RR19 pKa = 11.84NVLAEE24 pKa = 5.27LGDD27 pKa = 3.89QPTTQYY33 pKa = 11.14LIYY36 pKa = 9.68GDD38 pKa = 4.13EE39 pKa = 4.39VGASGTPHH47 pKa = 6.34LQGHH51 pKa = 5.35VSFVQRR57 pKa = 11.84YY58 pKa = 8.4RR59 pKa = 11.84FNQVKK64 pKa = 9.93NWVSPRR70 pKa = 11.84AHH72 pKa = 6.65LEE74 pKa = 3.67LVRR77 pKa = 11.84LLRR80 pKa = 11.84RR81 pKa = 11.84HH82 pKa = 6.28IEE84 pKa = 3.93YY85 pKa = 10.32CKK87 pKa = 10.53KK88 pKa = 10.54DD89 pKa = 3.25GAYY92 pKa = 10.66LEE94 pKa = 5.39FGTPPDD100 pKa = 3.36STLAKK105 pKa = 10.15DD106 pKa = 4.01GKK108 pKa = 10.44RR109 pKa = 11.84NEE111 pKa = 4.15LAEE114 pKa = 4.12FRR116 pKa = 11.84ATVAEE121 pKa = 4.77GVFHH125 pKa = 7.27SPEE128 pKa = 3.93LRR130 pKa = 11.84EE131 pKa = 3.75KK132 pKa = 10.35HH133 pKa = 6.16PNVMARR139 pKa = 11.84YY140 pKa = 7.83PHH142 pKa = 6.62FANSIIRR149 pKa = 11.84DD150 pKa = 4.45LFPQSAPPDD159 pKa = 3.58LPLRR163 pKa = 11.84AWQQRR168 pKa = 11.84VVEE171 pKa = 5.37LIDD174 pKa = 4.6APPDD178 pKa = 3.34PRR180 pKa = 11.84KK181 pKa = 10.53VYY183 pKa = 10.19FIVDD187 pKa = 3.53RR188 pKa = 11.84QGNAGKK194 pKa = 8.76TSLAKK199 pKa = 10.38LLHH202 pKa = 5.93RR203 pKa = 11.84THH205 pKa = 6.7EE206 pKa = 4.21AVQIIRR212 pKa = 11.84SGKK215 pKa = 7.81VADD218 pKa = 3.49MAYY221 pKa = 10.33LYY223 pKa = 10.68KK224 pKa = 9.69ITTKK228 pKa = 10.48ILILDD233 pKa = 3.84VPRR236 pKa = 11.84SKK238 pKa = 11.17SEE240 pKa = 3.62LLQYY244 pKa = 11.17SFIEE248 pKa = 4.16MVKK251 pKa = 10.6DD252 pKa = 3.54GLLMSTKK259 pKa = 10.05YY260 pKa = 10.29EE261 pKa = 4.22SVMKK265 pKa = 10.38TFDD268 pKa = 3.76PPHH271 pKa = 7.68ILVMMNADD279 pKa = 4.04PDD281 pKa = 3.9HH282 pKa = 6.7TALSTDD288 pKa = 3.47RR289 pKa = 11.84YY290 pKa = 10.54HH291 pKa = 7.72YY292 pKa = 10.0IIPHH296 pKa = 6.01

Molecular weight: 34.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075LZ71|A0A075LZ71_9VIRU Replication-associated protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-1 OX=1521385 PE=4 SV=1
MM1 pKa = 7.64PKK3 pKa = 9.92VRR5 pKa = 11.84WDD7 pKa = 4.07DD8 pKa = 3.58VVKK11 pKa = 10.36WGIFMPWGRR20 pKa = 11.84SSLMRR25 pKa = 11.84HH26 pKa = 5.61WDD28 pKa = 3.45NDD30 pKa = 3.36YY31 pKa = 11.2KK32 pKa = 10.99QYY34 pKa = 11.24NDD36 pKa = 4.28PKK38 pKa = 9.52TIQYY42 pKa = 10.73KK43 pKa = 10.06DD44 pKa = 2.83GSMAKK49 pKa = 9.63RR50 pKa = 11.84KK51 pKa = 9.25SASVTVFGGQASSKK65 pKa = 9.46RR66 pKa = 11.84ARR68 pKa = 11.84TRR70 pKa = 11.84SMGRR74 pKa = 11.84IRR76 pKa = 11.84RR77 pKa = 11.84VTRR80 pKa = 11.84YY81 pKa = 9.22RR82 pKa = 11.84RR83 pKa = 11.84GRR85 pKa = 11.84RR86 pKa = 11.84GASRR90 pKa = 11.84KK91 pKa = 8.37RR92 pKa = 11.84TSSYY96 pKa = 9.44GARR99 pKa = 11.84ARR101 pKa = 11.84RR102 pKa = 11.84QIGEE106 pKa = 4.04RR107 pKa = 11.84VGTHH111 pKa = 4.24SAKK114 pKa = 10.15TKK116 pKa = 9.54VVEE119 pKa = 4.48DD120 pKa = 3.81AVTTFAMSTRR130 pKa = 11.84TLYY133 pKa = 11.07NLVVSEE139 pKa = 4.11VLEE142 pKa = 4.15GTEE145 pKa = 3.82RR146 pKa = 11.84NEE148 pKa = 4.28RR149 pKa = 11.84VNDD152 pKa = 3.56KK153 pKa = 11.23VNFRR157 pKa = 11.84GIKK160 pKa = 10.23VFLQAYY166 pKa = 7.49NALNTPVNFHH176 pKa = 5.86VAYY179 pKa = 10.5LSPKK183 pKa = 9.44VGQTIDD189 pKa = 3.19TTDD192 pKa = 3.89FFRR195 pKa = 11.84SEE197 pKa = 4.13RR198 pKa = 11.84IARR201 pKa = 11.84GRR203 pKa = 11.84NFATIEE209 pKa = 3.98LSSLEE214 pKa = 4.13MRR216 pKa = 11.84TLPINTDD223 pKa = 3.0RR224 pKa = 11.84YY225 pKa = 10.39VVLKK229 pKa = 9.92HH230 pKa = 4.89MRR232 pKa = 11.84MRR234 pKa = 11.84LAAATEE240 pKa = 3.85ASTIFEE246 pKa = 4.78DD247 pKa = 4.03GGRR250 pKa = 11.84PSLMTAQFYY259 pKa = 11.1LPIKK263 pKa = 9.22RR264 pKa = 11.84QLRR267 pKa = 11.84FEE269 pKa = 4.74GPGSDD274 pKa = 3.42TPIDD278 pKa = 4.18GKK280 pKa = 9.45SLWFIGATFLQQMLVLRR297 pKa = 11.84YY298 pKa = 9.36

Molecular weight: 34.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

793

199

298

264.3

30.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.305 ± 0.66

0.631 ± 0.516

4.414 ± 1.105

4.666 ± 0.349

4.035 ± 0.318

5.044 ± 1.118

2.774 ± 0.887

5.17 ± 0.537

6.053 ± 0.36

9.458 ± 1.768

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.279 ± 0.26

3.909 ± 0.413

5.422 ± 0.978

3.405 ± 0.763

8.323 ± 2.037

7.945 ± 1.742

6.81 ± 0.669

6.81 ± 0.332

1.765 ± 0.48

3.783 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski