Beihai sobemo-like virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KE94|A0A1L3KE94_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 21 OX=1922693 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84LCVSPFMVSCAIIIAVYY19 pKa = 6.83THH21 pKa = 6.61IGIMIGVMVGDD32 pKa = 4.15FTDD35 pKa = 3.83LPFRR39 pKa = 11.84EE40 pKa = 4.52LNKK43 pKa = 10.13QLLRR47 pKa = 11.84THH49 pKa = 6.01YY50 pKa = 10.6VHH52 pKa = 7.39AFWASLNFLGEE63 pKa = 4.53FLRR66 pKa = 11.84SMHH69 pKa = 5.89TFLWALVEE77 pKa = 5.94FILAQNFSLEE87 pKa = 4.37SVGFIALLVVPTIHH101 pKa = 5.7VLYY104 pKa = 10.84VIFRR108 pKa = 11.84PKK110 pKa = 10.09PIRR113 pKa = 11.84FGEE116 pKa = 4.01IEE118 pKa = 4.63EE119 pKa = 4.37ILDD122 pKa = 3.98GYY124 pKa = 11.0LAFTGEE130 pKa = 4.42SLQEE134 pKa = 4.0GSPLQQVQPEE144 pKa = 4.44SVPKK148 pKa = 10.14GQAVLIAEE156 pKa = 4.52TATARR161 pKa = 11.84YY162 pKa = 8.55LQGQVTRR169 pKa = 11.84MDD171 pKa = 3.59NVEE174 pKa = 4.0VTDD177 pKa = 3.87TVIEE181 pKa = 4.14RR182 pKa = 11.84WNKK185 pKa = 10.45LGIDD189 pKa = 3.66YY190 pKa = 10.72DD191 pKa = 4.2FSIVNSGEE199 pKa = 4.26TKK201 pKa = 9.11FACYY205 pKa = 9.97AGLSHH210 pKa = 6.74CTTRR214 pKa = 11.84SGKK217 pKa = 9.93YY218 pKa = 10.04FIAKK222 pKa = 10.29GNDD225 pKa = 3.15LNVQVPCYY233 pKa = 10.03SVPSLAAEE241 pKa = 3.96DD242 pKa = 4.4LMFFLVPANKK252 pKa = 9.76VSVLGNPVVKK262 pKa = 9.93PHH264 pKa = 6.15STDD267 pKa = 2.85LPANVRR273 pKa = 11.84VITSAAKK280 pKa = 9.4YY281 pKa = 10.05GEE283 pKa = 4.5KK284 pKa = 10.19FGSWSTFGRR293 pKa = 11.84LDD295 pKa = 3.68RR296 pKa = 11.84DD297 pKa = 4.12DD298 pKa = 3.68VTGCCRR304 pKa = 11.84FTGSTISGYY313 pKa = 10.78SGAGYY318 pKa = 9.49YY319 pKa = 10.57ASNAWVGIHH328 pKa = 6.53RR329 pKa = 11.84GHH331 pKa = 7.37RR332 pKa = 11.84SGLNFGFLAAYY343 pKa = 9.38VKK345 pKa = 9.27TVKK348 pKa = 10.55NVTFLDD354 pKa = 3.71KK355 pKa = 11.01AVTGEE360 pKa = 4.17SDD362 pKa = 3.43TEE364 pKa = 4.05LARR367 pKa = 11.84LLGLLRR373 pKa = 11.84EE374 pKa = 5.04DD375 pKa = 4.55GPMAYY380 pKa = 10.12SKK382 pKa = 10.77LSKK385 pKa = 10.15RR386 pKa = 11.84KK387 pKa = 10.27SGDD390 pKa = 3.24PSIDD394 pKa = 3.2LYY396 pKa = 11.03EE397 pKa = 4.05YY398 pKa = 10.74NGDD401 pKa = 3.47YY402 pKa = 10.63FYY404 pKa = 11.76VDD406 pKa = 3.36RR407 pKa = 11.84DD408 pKa = 3.99DD409 pKa = 3.94VNSYY413 pKa = 8.44QRR415 pKa = 11.84EE416 pKa = 3.75EE417 pKa = 3.85WQNYY421 pKa = 7.89LDD423 pKa = 4.87AMQMDD428 pKa = 4.8NEE430 pKa = 4.44TEE432 pKa = 3.84DD433 pKa = 3.49RR434 pKa = 11.84QVRR437 pKa = 11.84RR438 pKa = 11.84RR439 pKa = 11.84NKK441 pKa = 9.53SRR443 pKa = 11.84RR444 pKa = 11.84RR445 pKa = 11.84DD446 pKa = 3.49DD447 pKa = 3.41TDD449 pKa = 3.76DD450 pKa = 4.17FEE452 pKa = 7.14GEE454 pKa = 4.09AGSVDD459 pKa = 3.87LTHH462 pKa = 6.84LNSRR466 pKa = 11.84GGAVRR471 pKa = 11.84TEE473 pKa = 4.28CGEE476 pKa = 4.2EE477 pKa = 3.76IQGTMLLPPPRR488 pKa = 11.84CDD490 pKa = 2.86STGNVTDD497 pKa = 3.57HH498 pKa = 6.71ARR500 pKa = 11.84EE501 pKa = 3.8KK502 pKa = 10.72RR503 pKa = 11.84ADD505 pKa = 4.17EE506 pKa = 4.1IALLLIKK513 pKa = 10.53QKK515 pKa = 10.83LDD517 pKa = 3.34RR518 pKa = 11.84LSEE521 pKa = 4.01KK522 pKa = 9.91MKK524 pKa = 10.74QSFVNYY530 pKa = 9.11TFEE533 pKa = 5.17QKK535 pKa = 10.49LGWMKK540 pKa = 10.55SPQEE544 pKa = 3.84LWHH547 pKa = 6.27LTGKK551 pKa = 8.82MLSIQSALTHH561 pKa = 6.5WIKK564 pKa = 10.56PVAEE568 pKa = 4.85AYY570 pKa = 10.28SEE572 pKa = 4.25TMSRR576 pKa = 11.84VAEE579 pKa = 3.91LTPWEE584 pKa = 4.13EE585 pKa = 3.8EE586 pKa = 4.13AKK588 pKa = 10.63KK589 pKa = 10.67KK590 pKa = 9.58LAKK593 pKa = 10.25SRR595 pKa = 11.84ASYY598 pKa = 10.94NKK600 pKa = 10.53ANGTPAPPASFDD612 pKa = 4.61PILEE616 pKa = 4.08SWYY619 pKa = 9.83EE620 pKa = 3.9ATEE623 pKa = 4.01KK624 pKa = 10.61LHH626 pKa = 5.13EE627 pKa = 4.47QIMEE631 pKa = 3.69ISEE634 pKa = 4.58RR635 pKa = 11.84YY636 pKa = 8.24PPPSVTTEE644 pKa = 3.9GDD646 pKa = 3.44VADD649 pKa = 4.69PEE651 pKa = 4.54DD652 pKa = 4.39FEE654 pKa = 6.48GEE656 pKa = 4.33TGDD659 pKa = 4.46HH660 pKa = 5.69QLEE663 pKa = 4.38DD664 pKa = 3.65LLEE667 pKa = 4.18ILRR670 pKa = 11.84NPTQPPLTQPLEE682 pKa = 4.17KK683 pKa = 10.1EE684 pKa = 3.95QTSRR688 pKa = 11.84FQMKK692 pKa = 9.28HH693 pKa = 5.61LSGAPIVKK701 pKa = 8.21QTAAKK706 pKa = 10.44SPTTLPVVPAVISSGDD722 pKa = 3.79DD723 pKa = 3.5LEE725 pKa = 5.08VGADD729 pKa = 3.48AEE731 pKa = 4.63SFSASQAVSHH741 pKa = 6.09PTEE744 pKa = 4.34DD745 pKa = 3.93FIEE748 pKa = 4.72RR749 pKa = 11.84SSLTSQATSRR759 pKa = 11.84PPVLSDD765 pKa = 3.31RR766 pKa = 11.84GQRR769 pKa = 11.84HH770 pKa = 5.82ASASNQLEE778 pKa = 4.3TPLASNGQIQNDD790 pKa = 3.82QPTQLPKK797 pKa = 10.68ASAIIPEE804 pKa = 4.69CSSEE808 pKa = 4.25TTSQMSGPHH817 pKa = 5.81GMNLTKK823 pKa = 10.37SAKK826 pKa = 9.14TSGKK830 pKa = 10.23SSLSSPRR837 pKa = 11.84QEE839 pKa = 4.44SRR841 pKa = 11.84FEE843 pKa = 4.29TVWPEE848 pKa = 3.76KK849 pKa = 10.95SKK851 pKa = 9.82LTSNKK856 pKa = 9.81KK857 pKa = 9.97LVSLLTMVEE866 pKa = 3.92RR867 pKa = 11.84PDD869 pKa = 3.23WVRR872 pKa = 11.84QADD875 pKa = 3.58WDD877 pKa = 3.79KK878 pKa = 11.46LINKK882 pKa = 9.53RR883 pKa = 11.84LFLRR887 pKa = 11.84VNLLTDD893 pKa = 3.5TCLQEE898 pKa = 4.17YY899 pKa = 10.47CLTRR903 pKa = 11.84PSWSHH908 pKa = 6.73QYY910 pKa = 8.45TQDD913 pKa = 2.66QWEE916 pKa = 4.98DD917 pKa = 3.6YY918 pKa = 10.17LQKK921 pKa = 10.7EE922 pKa = 4.45SQAQQLVSLKK932 pKa = 8.93TQLEE936 pKa = 4.59KK937 pKa = 10.49IQQQADD943 pKa = 3.62SHH945 pKa = 6.56KK946 pKa = 10.6GSARR950 pKa = 11.84QPQAQKK956 pKa = 10.59QKK958 pKa = 10.69KK959 pKa = 9.7KK960 pKa = 10.35KK961 pKa = 9.88GKK963 pKa = 8.66QANATPVAEE972 pKa = 4.3PTTSNN977 pKa = 3.29

Molecular weight:
109.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KE94|A0A1L3KE94_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 21 OX=1922693 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 4.35EE3 pKa = 4.19VTSRR7 pKa = 11.84TLAPHH12 pKa = 6.59RR13 pKa = 11.84KK14 pKa = 9.12DD15 pKa = 3.09VEE17 pKa = 4.18YY18 pKa = 9.88PKK20 pKa = 10.87RR21 pKa = 11.84LDD23 pKa = 3.91TLDD26 pKa = 3.56QACSRR31 pKa = 11.84GLLRR35 pKa = 11.84DD36 pKa = 3.64NVEE39 pKa = 4.12SGGTHH44 pKa = 4.22TMGRR48 pKa = 11.84RR49 pKa = 11.84SEE51 pKa = 4.17EE52 pKa = 3.91EE53 pKa = 3.55TGKK56 pKa = 10.1IARR59 pKa = 11.84KK60 pKa = 8.58LQQSEE65 pKa = 4.61RR66 pKa = 11.84NASTSCVIRR75 pKa = 11.84PNSGVLVRR83 pKa = 11.84GNGKK87 pKa = 7.09TARR90 pKa = 11.84TDD92 pKa = 3.29NGDD95 pKa = 3.23QRR97 pKa = 11.84TLSATICDD105 pKa = 3.54NRR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84CGPGRR115 pKa = 11.84LRR117 pKa = 11.84RR118 pKa = 11.84GNGRR122 pKa = 11.84SSARR126 pKa = 11.84RR127 pKa = 11.84PVRR130 pKa = 11.84NSEE133 pKa = 4.07KK134 pKa = 10.44PNPTTVDD141 pKa = 3.25STIGEE146 pKa = 4.29GADD149 pKa = 3.11IEE151 pKa = 4.52ISNEE155 pKa = 3.73APFRR159 pKa = 11.84CPDD162 pKa = 3.62CEE164 pKa = 4.05TDD166 pKa = 3.3CRR168 pKa = 11.84KK169 pKa = 8.75ITDD172 pKa = 3.54YY173 pKa = 7.48TTRR176 pKa = 11.84CPSCDD181 pKa = 3.54KK182 pKa = 10.15QWRR185 pKa = 11.84RR186 pKa = 11.84LGGRR190 pKa = 11.84CGCRR194 pKa = 11.84EE195 pKa = 4.01LFGVPGCLTSYY206 pKa = 10.51RR207 pKa = 11.84GFHH210 pKa = 6.41RR211 pKa = 11.84KK212 pKa = 8.79IKK214 pKa = 10.36FDD216 pKa = 3.67FSSYY220 pKa = 10.64VPATCPLRR228 pKa = 11.84QGAEE232 pKa = 3.73TCEE235 pKa = 4.25CFKK238 pKa = 11.23SIGDD242 pKa = 3.6PAGFQWPDD250 pKa = 3.3PEE252 pKa = 5.61RR253 pKa = 11.84PTNPIAEE260 pKa = 4.72SICYY264 pKa = 9.87HH265 pKa = 6.11SGVFLRR271 pKa = 11.84NYY273 pKa = 9.86KK274 pKa = 10.09PDD276 pKa = 3.3VRR278 pKa = 11.84PTRR281 pKa = 11.84DD282 pKa = 3.69EE283 pKa = 4.23LDD285 pKa = 3.71QVCKK289 pKa = 10.73DD290 pKa = 3.18LWKK293 pKa = 10.42KK294 pKa = 10.41FSIFASPRR302 pKa = 11.84KK303 pKa = 9.1PVRR306 pKa = 11.84DD307 pKa = 3.54CLAGKK312 pKa = 8.76EE313 pKa = 3.98QTYY316 pKa = 9.94LQQEE320 pKa = 4.33VSKK323 pKa = 10.62PLDD326 pKa = 4.16DD327 pKa = 4.69GGKK330 pKa = 7.43TRR332 pKa = 11.84LGATSRR338 pKa = 11.84LGQVDD343 pKa = 3.7QQTSFPEE350 pKa = 4.05SEE352 pKa = 4.44PLDD355 pKa = 4.19GYY357 pKa = 11.03LPTGILPDD365 pKa = 3.52QTEE368 pKa = 4.75LEE370 pKa = 4.19SSIYY374 pKa = 9.1SRR376 pKa = 11.84PMGGLSSEE384 pKa = 4.53GEE386 pKa = 4.33SGSTTGEE393 pKa = 4.23LEE395 pKa = 3.96NSTGEE400 pKa = 4.05NPTTGGFPQGICSSTPGSKK419 pKa = 10.39AEE421 pKa = 4.14EE422 pKa = 4.44KK423 pKa = 10.31EE424 pKa = 4.07RR425 pKa = 11.84EE426 pKa = 4.13AGKK429 pKa = 10.4CYY431 pKa = 9.88PGCRR435 pKa = 11.84TDD437 pKa = 4.31HH438 pKa = 5.87EE439 pKa = 4.36QLVRR443 pKa = 11.84GILPSFDD450 pKa = 5.18DD451 pKa = 4.01FTTLVNRR458 pKa = 11.84QLDD461 pKa = 3.93KK462 pKa = 11.46NSTPGIPYY470 pKa = 8.45NTIAPTNAEE479 pKa = 3.93VFGWDD484 pKa = 3.23GYY486 pKa = 11.65KK487 pKa = 8.9YY488 pKa = 9.35TKK490 pKa = 10.45NLDD493 pKa = 3.25MLYY496 pKa = 10.4NDD498 pKa = 3.31VFEE501 pKa = 5.57RR502 pKa = 11.84MVDD505 pKa = 3.91LLSGPASNPINLFLKK520 pKa = 10.32PEE522 pKa = 3.83PHH524 pKa = 6.83KK525 pKa = 10.76KK526 pKa = 9.99SKK528 pKa = 10.94CEE530 pKa = 3.57AGAWRR535 pKa = 11.84IIWGCSCVDD544 pKa = 3.27QMVALVLWYY553 pKa = 9.85PNVDD557 pKa = 3.51KK558 pKa = 10.91IVNQWGMSPSMIGWTPQRR576 pKa = 11.84GGHH579 pKa = 5.7KK580 pKa = 9.52WLSEE584 pKa = 4.06LFNARR589 pKa = 11.84HH590 pKa = 6.06EE591 pKa = 4.55SVQCADD597 pKa = 3.11KK598 pKa = 11.18SAWDD602 pKa = 3.33WTVQFWVCVAVSYY615 pKa = 10.88VLVNLMVGASEE626 pKa = 4.04AQRR629 pKa = 11.84VIAHH633 pKa = 5.57NHH635 pKa = 4.66CMAMLGYY642 pKa = 8.45QTVQFGKK649 pKa = 10.53KK650 pKa = 9.04KK651 pKa = 10.74LKK653 pKa = 9.96FQIPGRR659 pKa = 11.84LPSGWFWTIFFNCAGQASVDD679 pKa = 3.7SLASQRR685 pKa = 11.84VGEE688 pKa = 4.31VPDD691 pKa = 4.47PKK693 pKa = 10.48LKK695 pKa = 11.17AMGDD699 pKa = 3.59DD700 pKa = 3.47TAQTVRR706 pKa = 11.84SDD708 pKa = 4.17AYY710 pKa = 9.1WKK712 pKa = 10.54AVNQLCIVKK721 pKa = 10.48QIDD724 pKa = 3.52TFKK727 pKa = 10.93PGDD730 pKa = 3.55EE731 pKa = 4.69FEE733 pKa = 4.55FCGTLMNHH741 pKa = 6.09EE742 pKa = 4.88RR743 pKa = 11.84GTPAKK748 pKa = 9.75RR749 pKa = 11.84GSHH752 pKa = 6.45AFRR755 pKa = 11.84LHH757 pKa = 6.76FLRR760 pKa = 11.84DD761 pKa = 3.08EE762 pKa = 4.45CAAEE766 pKa = 4.12GLVSYY771 pKa = 10.98ALLYY775 pKa = 10.87AHH777 pKa = 7.48DD778 pKa = 4.32PAAFATISALQCEE791 pKa = 4.61IGRR794 pKa = 11.84YY795 pKa = 7.35DD796 pKa = 3.55TLITRR801 pKa = 11.84DD802 pKa = 3.86AAVAWYY808 pKa = 10.19SGTALGIGG816 pKa = 3.61

Molecular weight:
90.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1793

816

977

896.5

100.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.86 ± 0.244

2.454 ± 0.981

5.745 ± 0.256

6.581 ± 0.467

3.625 ± 0.034

7.195 ± 1.089

1.952 ± 0.158

3.96 ± 0.026

5.521 ± 0.25

8.254 ± 0.767

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.84 ± 0.165

3.569 ± 0.072

5.745 ± 0.256

4.908 ± 0.578

6.247 ± 0.822

8.199 ± 0.565

6.804 ± 0.043

5.856 ± 0.31

1.84 ± 0.162

2.844 ± 0.099

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski