Beihai sobemo-like virus 21
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KE94|A0A1L3KE94_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 21 OX=1922693 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 LCVSPFMVSCAIIIAVYY19 pKa = 6.83 THH21 pKa = 6.61 IGIMIGVMVGDD32 pKa = 4.15 FTDD35 pKa = 3.83 LPFRR39 pKa = 11.84 EE40 pKa = 4.52 LNKK43 pKa = 10.13 QLLRR47 pKa = 11.84 THH49 pKa = 6.01 YY50 pKa = 10.6 VHH52 pKa = 7.39 AFWASLNFLGEE63 pKa = 4.53 FLRR66 pKa = 11.84 SMHH69 pKa = 5.89 TFLWALVEE77 pKa = 5.94 FILAQNFSLEE87 pKa = 4.37 SVGFIALLVVPTIHH101 pKa = 5.7 VLYY104 pKa = 10.84 VIFRR108 pKa = 11.84 PKK110 pKa = 10.09 PIRR113 pKa = 11.84 FGEE116 pKa = 4.01 IEE118 pKa = 4.63 EE119 pKa = 4.37 ILDD122 pKa = 3.98 GYY124 pKa = 11.0 LAFTGEE130 pKa = 4.42 SLQEE134 pKa = 4.0 GSPLQQVQPEE144 pKa = 4.44 SVPKK148 pKa = 10.14 GQAVLIAEE156 pKa = 4.52 TATARR161 pKa = 11.84 YY162 pKa = 8.55 LQGQVTRR169 pKa = 11.84 MDD171 pKa = 3.59 NVEE174 pKa = 4.0 VTDD177 pKa = 3.87 TVIEE181 pKa = 4.14 RR182 pKa = 11.84 WNKK185 pKa = 10.45 LGIDD189 pKa = 3.66 YY190 pKa = 10.72 DD191 pKa = 4.2 FSIVNSGEE199 pKa = 4.26 TKK201 pKa = 9.11 FACYY205 pKa = 9.97 AGLSHH210 pKa = 6.74 CTTRR214 pKa = 11.84 SGKK217 pKa = 9.93 YY218 pKa = 10.04 FIAKK222 pKa = 10.29 GNDD225 pKa = 3.15 LNVQVPCYY233 pKa = 10.03 SVPSLAAEE241 pKa = 3.96 DD242 pKa = 4.4 LMFFLVPANKK252 pKa = 9.76 VSVLGNPVVKK262 pKa = 9.93 PHH264 pKa = 6.15 STDD267 pKa = 2.85 LPANVRR273 pKa = 11.84 VITSAAKK280 pKa = 9.4 YY281 pKa = 10.05 GEE283 pKa = 4.5 KK284 pKa = 10.19 FGSWSTFGRR293 pKa = 11.84 LDD295 pKa = 3.68 RR296 pKa = 11.84 DD297 pKa = 4.12 DD298 pKa = 3.68 VTGCCRR304 pKa = 11.84 FTGSTISGYY313 pKa = 10.78 SGAGYY318 pKa = 9.49 YY319 pKa = 10.57 ASNAWVGIHH328 pKa = 6.53 RR329 pKa = 11.84 GHH331 pKa = 7.37 RR332 pKa = 11.84 SGLNFGFLAAYY343 pKa = 9.38 VKK345 pKa = 9.27 TVKK348 pKa = 10.55 NVTFLDD354 pKa = 3.71 KK355 pKa = 11.01 AVTGEE360 pKa = 4.17 SDD362 pKa = 3.43 TEE364 pKa = 4.05 LARR367 pKa = 11.84 LLGLLRR373 pKa = 11.84 EE374 pKa = 5.04 DD375 pKa = 4.55 GPMAYY380 pKa = 10.12 SKK382 pKa = 10.77 LSKK385 pKa = 10.15 RR386 pKa = 11.84 KK387 pKa = 10.27 SGDD390 pKa = 3.24 PSIDD394 pKa = 3.2 LYY396 pKa = 11.03 EE397 pKa = 4.05 YY398 pKa = 10.74 NGDD401 pKa = 3.47 YY402 pKa = 10.63 FYY404 pKa = 11.76 VDD406 pKa = 3.36 RR407 pKa = 11.84 DD408 pKa = 3.99 DD409 pKa = 3.94 VNSYY413 pKa = 8.44 QRR415 pKa = 11.84 EE416 pKa = 3.75 EE417 pKa = 3.85 WQNYY421 pKa = 7.89 LDD423 pKa = 4.87 AMQMDD428 pKa = 4.8 NEE430 pKa = 4.44 TEE432 pKa = 3.84 DD433 pKa = 3.49 RR434 pKa = 11.84 QVRR437 pKa = 11.84 RR438 pKa = 11.84 RR439 pKa = 11.84 NKK441 pKa = 9.53 SRR443 pKa = 11.84 RR444 pKa = 11.84 RR445 pKa = 11.84 DD446 pKa = 3.49 DD447 pKa = 3.41 TDD449 pKa = 3.76 DD450 pKa = 4.17 FEE452 pKa = 7.14 GEE454 pKa = 4.09 AGSVDD459 pKa = 3.87 LTHH462 pKa = 6.84 LNSRR466 pKa = 11.84 GGAVRR471 pKa = 11.84 TEE473 pKa = 4.28 CGEE476 pKa = 4.2 EE477 pKa = 3.76 IQGTMLLPPPRR488 pKa = 11.84 CDD490 pKa = 2.86 STGNVTDD497 pKa = 3.57 HH498 pKa = 6.71 ARR500 pKa = 11.84 EE501 pKa = 3.8 KK502 pKa = 10.72 RR503 pKa = 11.84 ADD505 pKa = 4.17 EE506 pKa = 4.1 IALLLIKK513 pKa = 10.53 QKK515 pKa = 10.83 LDD517 pKa = 3.34 RR518 pKa = 11.84 LSEE521 pKa = 4.01 KK522 pKa = 9.91 MKK524 pKa = 10.74 QSFVNYY530 pKa = 9.11 TFEE533 pKa = 5.17 QKK535 pKa = 10.49 LGWMKK540 pKa = 10.55 SPQEE544 pKa = 3.84 LWHH547 pKa = 6.27 LTGKK551 pKa = 8.82 MLSIQSALTHH561 pKa = 6.5 WIKK564 pKa = 10.56 PVAEE568 pKa = 4.85 AYY570 pKa = 10.28 SEE572 pKa = 4.25 TMSRR576 pKa = 11.84 VAEE579 pKa = 3.91 LTPWEE584 pKa = 4.13 EE585 pKa = 3.8 EE586 pKa = 4.13 AKK588 pKa = 10.63 KK589 pKa = 10.67 KK590 pKa = 9.58 LAKK593 pKa = 10.25 SRR595 pKa = 11.84 ASYY598 pKa = 10.94 NKK600 pKa = 10.53 ANGTPAPPASFDD612 pKa = 4.61 PILEE616 pKa = 4.08 SWYY619 pKa = 9.83 EE620 pKa = 3.9 ATEE623 pKa = 4.01 KK624 pKa = 10.61 LHH626 pKa = 5.13 EE627 pKa = 4.47 QIMEE631 pKa = 3.69 ISEE634 pKa = 4.58 RR635 pKa = 11.84 YY636 pKa = 8.24 PPPSVTTEE644 pKa = 3.9 GDD646 pKa = 3.44 VADD649 pKa = 4.69 PEE651 pKa = 4.54 DD652 pKa = 4.39 FEE654 pKa = 6.48 GEE656 pKa = 4.33 TGDD659 pKa = 4.46 HH660 pKa = 5.69 QLEE663 pKa = 4.38 DD664 pKa = 3.65 LLEE667 pKa = 4.18 ILRR670 pKa = 11.84 NPTQPPLTQPLEE682 pKa = 4.17 KK683 pKa = 10.1 EE684 pKa = 3.95 QTSRR688 pKa = 11.84 FQMKK692 pKa = 9.28 HH693 pKa = 5.61 LSGAPIVKK701 pKa = 8.21 QTAAKK706 pKa = 10.44 SPTTLPVVPAVISSGDD722 pKa = 3.79 DD723 pKa = 3.5 LEE725 pKa = 5.08 VGADD729 pKa = 3.48 AEE731 pKa = 4.63 SFSASQAVSHH741 pKa = 6.09 PTEE744 pKa = 4.34 DD745 pKa = 3.93 FIEE748 pKa = 4.72 RR749 pKa = 11.84 SSLTSQATSRR759 pKa = 11.84 PPVLSDD765 pKa = 3.31 RR766 pKa = 11.84 GQRR769 pKa = 11.84 HH770 pKa = 5.82 ASASNQLEE778 pKa = 4.3 TPLASNGQIQNDD790 pKa = 3.82 QPTQLPKK797 pKa = 10.68 ASAIIPEE804 pKa = 4.69 CSSEE808 pKa = 4.25 TTSQMSGPHH817 pKa = 5.81 GMNLTKK823 pKa = 10.37 SAKK826 pKa = 9.14 TSGKK830 pKa = 10.23 SSLSSPRR837 pKa = 11.84 QEE839 pKa = 4.44 SRR841 pKa = 11.84 FEE843 pKa = 4.29 TVWPEE848 pKa = 3.76 KK849 pKa = 10.95 SKK851 pKa = 9.82 LTSNKK856 pKa = 9.81 KK857 pKa = 9.97 LVSLLTMVEE866 pKa = 3.92 RR867 pKa = 11.84 PDD869 pKa = 3.23 WVRR872 pKa = 11.84 QADD875 pKa = 3.58 WDD877 pKa = 3.79 KK878 pKa = 11.46 LINKK882 pKa = 9.53 RR883 pKa = 11.84 LFLRR887 pKa = 11.84 VNLLTDD893 pKa = 3.5 TCLQEE898 pKa = 4.17 YY899 pKa = 10.47 CLTRR903 pKa = 11.84 PSWSHH908 pKa = 6.73 QYY910 pKa = 8.45 TQDD913 pKa = 2.66 QWEE916 pKa = 4.98 DD917 pKa = 3.6 YY918 pKa = 10.17 LQKK921 pKa = 10.7 EE922 pKa = 4.45 SQAQQLVSLKK932 pKa = 8.93 TQLEE936 pKa = 4.59 KK937 pKa = 10.49 IQQQADD943 pKa = 3.62 SHH945 pKa = 6.56 KK946 pKa = 10.6 GSARR950 pKa = 11.84 QPQAQKK956 pKa = 10.59 QKK958 pKa = 10.69 KK959 pKa = 9.7 KK960 pKa = 10.35 KK961 pKa = 9.88 GKK963 pKa = 8.66 QANATPVAEE972 pKa = 4.3 PTTSNN977 pKa = 3.29
Molecular weight: 109.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.649
IPC2_protein 5.614
IPC_protein 5.639
Toseland 5.97
ProMoST 5.944
Dawson 5.83
Bjellqvist 5.881
Wikipedia 5.817
Rodwell 5.804
Grimsley 6.097
Solomon 5.842
Lehninger 5.817
Nozaki 6.071
DTASelect 6.249
Thurlkill 6.224
EMBOSS 6.186
Sillero 6.173
Patrickios 4.279
IPC_peptide 5.855
IPC2_peptide 6.16
IPC2.peptide.svr19 6.163
Protein with the highest isoelectric point:
>tr|A0A1L3KE94|A0A1L3KE94_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 21 OX=1922693 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 4.35 EE3 pKa = 4.19 VTSRR7 pKa = 11.84 TLAPHH12 pKa = 6.59 RR13 pKa = 11.84 KK14 pKa = 9.12 DD15 pKa = 3.09 VEE17 pKa = 4.18 YY18 pKa = 9.88 PKK20 pKa = 10.87 RR21 pKa = 11.84 LDD23 pKa = 3.91 TLDD26 pKa = 3.56 QACSRR31 pKa = 11.84 GLLRR35 pKa = 11.84 DD36 pKa = 3.64 NVEE39 pKa = 4.12 SGGTHH44 pKa = 4.22 TMGRR48 pKa = 11.84 RR49 pKa = 11.84 SEE51 pKa = 4.17 EE52 pKa = 3.91 EE53 pKa = 3.55 TGKK56 pKa = 10.1 IARR59 pKa = 11.84 KK60 pKa = 8.58 LQQSEE65 pKa = 4.61 RR66 pKa = 11.84 NASTSCVIRR75 pKa = 11.84 PNSGVLVRR83 pKa = 11.84 GNGKK87 pKa = 7.09 TARR90 pKa = 11.84 TDD92 pKa = 3.29 NGDD95 pKa = 3.23 QRR97 pKa = 11.84 TLSATICDD105 pKa = 3.54 NRR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 CGPGRR115 pKa = 11.84 LRR117 pKa = 11.84 RR118 pKa = 11.84 GNGRR122 pKa = 11.84 SSARR126 pKa = 11.84 RR127 pKa = 11.84 PVRR130 pKa = 11.84 NSEE133 pKa = 4.07 KK134 pKa = 10.44 PNPTTVDD141 pKa = 3.25 STIGEE146 pKa = 4.29 GADD149 pKa = 3.11 IEE151 pKa = 4.52 ISNEE155 pKa = 3.73 APFRR159 pKa = 11.84 CPDD162 pKa = 3.62 CEE164 pKa = 4.05 TDD166 pKa = 3.3 CRR168 pKa = 11.84 KK169 pKa = 8.75 ITDD172 pKa = 3.54 YY173 pKa = 7.48 TTRR176 pKa = 11.84 CPSCDD181 pKa = 3.54 KK182 pKa = 10.15 QWRR185 pKa = 11.84 RR186 pKa = 11.84 LGGRR190 pKa = 11.84 CGCRR194 pKa = 11.84 EE195 pKa = 4.01 LFGVPGCLTSYY206 pKa = 10.51 RR207 pKa = 11.84 GFHH210 pKa = 6.41 RR211 pKa = 11.84 KK212 pKa = 8.79 IKK214 pKa = 10.36 FDD216 pKa = 3.67 FSSYY220 pKa = 10.64 VPATCPLRR228 pKa = 11.84 QGAEE232 pKa = 3.73 TCEE235 pKa = 4.25 CFKK238 pKa = 11.23 SIGDD242 pKa = 3.6 PAGFQWPDD250 pKa = 3.3 PEE252 pKa = 5.61 RR253 pKa = 11.84 PTNPIAEE260 pKa = 4.72 SICYY264 pKa = 9.87 HH265 pKa = 6.11 SGVFLRR271 pKa = 11.84 NYY273 pKa = 9.86 KK274 pKa = 10.09 PDD276 pKa = 3.3 VRR278 pKa = 11.84 PTRR281 pKa = 11.84 DD282 pKa = 3.69 EE283 pKa = 4.23 LDD285 pKa = 3.71 QVCKK289 pKa = 10.73 DD290 pKa = 3.18 LWKK293 pKa = 10.42 KK294 pKa = 10.41 FSIFASPRR302 pKa = 11.84 KK303 pKa = 9.1 PVRR306 pKa = 11.84 DD307 pKa = 3.54 CLAGKK312 pKa = 8.76 EE313 pKa = 3.98 QTYY316 pKa = 9.94 LQQEE320 pKa = 4.33 VSKK323 pKa = 10.62 PLDD326 pKa = 4.16 DD327 pKa = 4.69 GGKK330 pKa = 7.43 TRR332 pKa = 11.84 LGATSRR338 pKa = 11.84 LGQVDD343 pKa = 3.7 QQTSFPEE350 pKa = 4.05 SEE352 pKa = 4.44 PLDD355 pKa = 4.19 GYY357 pKa = 11.03 LPTGILPDD365 pKa = 3.52 QTEE368 pKa = 4.75 LEE370 pKa = 4.19 SSIYY374 pKa = 9.1 SRR376 pKa = 11.84 PMGGLSSEE384 pKa = 4.53 GEE386 pKa = 4.33 SGSTTGEE393 pKa = 4.23 LEE395 pKa = 3.96 NSTGEE400 pKa = 4.05 NPTTGGFPQGICSSTPGSKK419 pKa = 10.39 AEE421 pKa = 4.14 EE422 pKa = 4.44 KK423 pKa = 10.31 EE424 pKa = 4.07 RR425 pKa = 11.84 EE426 pKa = 4.13 AGKK429 pKa = 10.4 CYY431 pKa = 9.88 PGCRR435 pKa = 11.84 TDD437 pKa = 4.31 HH438 pKa = 5.87 EE439 pKa = 4.36 QLVRR443 pKa = 11.84 GILPSFDD450 pKa = 5.18 DD451 pKa = 4.01 FTTLVNRR458 pKa = 11.84 QLDD461 pKa = 3.93 KK462 pKa = 11.46 NSTPGIPYY470 pKa = 8.45 NTIAPTNAEE479 pKa = 3.93 VFGWDD484 pKa = 3.23 GYY486 pKa = 11.65 KK487 pKa = 8.9 YY488 pKa = 9.35 TKK490 pKa = 10.45 NLDD493 pKa = 3.25 MLYY496 pKa = 10.4 NDD498 pKa = 3.31 VFEE501 pKa = 5.57 RR502 pKa = 11.84 MVDD505 pKa = 3.91 LLSGPASNPINLFLKK520 pKa = 10.32 PEE522 pKa = 3.83 PHH524 pKa = 6.83 KK525 pKa = 10.76 KK526 pKa = 9.99 SKK528 pKa = 10.94 CEE530 pKa = 3.57 AGAWRR535 pKa = 11.84 IIWGCSCVDD544 pKa = 3.27 QMVALVLWYY553 pKa = 9.85 PNVDD557 pKa = 3.51 KK558 pKa = 10.91 IVNQWGMSPSMIGWTPQRR576 pKa = 11.84 GGHH579 pKa = 5.7 KK580 pKa = 9.52 WLSEE584 pKa = 4.06 LFNARR589 pKa = 11.84 HH590 pKa = 6.06 EE591 pKa = 4.55 SVQCADD597 pKa = 3.11 KK598 pKa = 11.18 SAWDD602 pKa = 3.33 WTVQFWVCVAVSYY615 pKa = 10.88 VLVNLMVGASEE626 pKa = 4.04 AQRR629 pKa = 11.84 VIAHH633 pKa = 5.57 NHH635 pKa = 4.66 CMAMLGYY642 pKa = 8.45 QTVQFGKK649 pKa = 10.53 KK650 pKa = 9.04 KK651 pKa = 10.74 LKK653 pKa = 9.96 FQIPGRR659 pKa = 11.84 LPSGWFWTIFFNCAGQASVDD679 pKa = 3.7 SLASQRR685 pKa = 11.84 VGEE688 pKa = 4.31 VPDD691 pKa = 4.47 PKK693 pKa = 10.48 LKK695 pKa = 11.17 AMGDD699 pKa = 3.59 DD700 pKa = 3.47 TAQTVRR706 pKa = 11.84 SDD708 pKa = 4.17 AYY710 pKa = 9.1 WKK712 pKa = 10.54 AVNQLCIVKK721 pKa = 10.48 QIDD724 pKa = 3.52 TFKK727 pKa = 10.93 PGDD730 pKa = 3.55 EE731 pKa = 4.69 FEE733 pKa = 4.55 FCGTLMNHH741 pKa = 6.09 EE742 pKa = 4.88 RR743 pKa = 11.84 GTPAKK748 pKa = 9.75 RR749 pKa = 11.84 GSHH752 pKa = 6.45 AFRR755 pKa = 11.84 LHH757 pKa = 6.76 FLRR760 pKa = 11.84 DD761 pKa = 3.08 EE762 pKa = 4.45 CAAEE766 pKa = 4.12 GLVSYY771 pKa = 10.98 ALLYY775 pKa = 10.87 AHH777 pKa = 7.48 DD778 pKa = 4.32 PAAFATISALQCEE791 pKa = 4.61 IGRR794 pKa = 11.84 YY795 pKa = 7.35 DD796 pKa = 3.55 TLITRR801 pKa = 11.84 DD802 pKa = 3.86 AAVAWYY808 pKa = 10.19 SGTALGIGG816 pKa = 3.61
Molecular weight: 90.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.823
IPC2_protein 7.088
IPC_protein 6.883
Toseland 6.561
ProMoST 7.512
Dawson 7.571
Bjellqvist 8.083
Wikipedia 7.483
Rodwell 7.6
Grimsley 6.59
Solomon 7.6
Lehninger 7.614
Nozaki 8.419
DTASelect 7.775
Thurlkill 7.819
EMBOSS 7.805
Sillero 8.17
Patrickios 4.215
IPC_peptide 7.585
IPC2_peptide 7.366
IPC2.peptide.svr19 7.13
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1793
816
977
896.5
100.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.86 ± 0.244
2.454 ± 0.981
5.745 ± 0.256
6.581 ± 0.467
3.625 ± 0.034
7.195 ± 1.089
1.952 ± 0.158
3.96 ± 0.026
5.521 ± 0.25
8.254 ± 0.767
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.84 ± 0.165
3.569 ± 0.072
5.745 ± 0.256
4.908 ± 0.578
6.247 ± 0.822
8.199 ± 0.565
6.804 ± 0.043
5.856 ± 0.31
1.84 ± 0.162
2.844 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here