Chaco virus
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R174|A0A0D3R174_9RHAB Matrix OS=Chaco virus OX=1158189 PE=4 SV=1
MM1 pKa = 7.53 GEE3 pKa = 4.16 SPINNYY9 pKa = 9.54 HH10 pKa = 6.4 FSDD13 pKa = 4.67 FEE15 pKa = 4.44 MTDD18 pKa = 3.02 STLDD22 pKa = 3.44 LSEE25 pKa = 4.47 YY26 pKa = 10.89 NVLEE30 pKa = 4.14 INAIPEE36 pKa = 4.26 GLQQWEE42 pKa = 4.28 IKK44 pKa = 10.13 SRR46 pKa = 11.84 QIDD49 pKa = 3.84 NILYY53 pKa = 10.57 ALAQLGIRR61 pKa = 11.84 CDD63 pKa = 3.85 YY64 pKa = 11.16 LVDD67 pKa = 4.26 DD68 pKa = 4.33 VDD70 pKa = 4.6 SIKK73 pKa = 10.77 FKK75 pKa = 11.26 LDD77 pKa = 3.46 FDD79 pKa = 4.61 PPLYY83 pKa = 10.61 SYY85 pKa = 11.13 EE86 pKa = 4.63 RR87 pKa = 11.84 DD88 pKa = 3.49 TDD90 pKa = 3.6 DD91 pKa = 3.59 SFIYY95 pKa = 10.48 FDD97 pKa = 5.05 EE98 pKa = 5.52 PDD100 pKa = 4.3 DD101 pKa = 5.5 LDD103 pKa = 5.69 DD104 pKa = 6.31 LFTLIDD110 pKa = 5.33 DD111 pKa = 4.06 EE112 pKa = 4.73 EE113 pKa = 4.4 FWW115 pKa = 5.16
Molecular weight: 13.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.503
IPC_protein 3.516
Toseland 3.287
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.478
Rodwell 3.338
Grimsley 3.198
Solomon 3.503
Lehninger 3.452
Nozaki 3.643
DTASelect 3.897
Thurlkill 3.35
EMBOSS 3.49
Sillero 3.643
Patrickios 0.896
IPC_peptide 3.49
IPC2_peptide 3.605
IPC2.peptide.svr19 3.642
Protein with the highest isoelectric point:
>tr|A0A0D3R1M6|A0A0D3R1M6_9RHAB Phosphoprotein OS=Chaco virus OX=1158189 PE=4 SV=1
MM1 pKa = 7.78 IILPIEE7 pKa = 3.95 MFAPFIVIAFKK18 pKa = 10.67 RR19 pKa = 11.84 IRR21 pKa = 11.84 KK22 pKa = 9.38 IIIKK26 pKa = 10.02 FLLATCANLNVQGEE40 pKa = 4.36 IKK42 pKa = 10.36 EE43 pKa = 4.09 RR44 pKa = 11.84 FYY46 pKa = 11.39 LYY48 pKa = 9.27 VTGMWEE54 pKa = 4.11 IYY56 pKa = 9.86 RR57 pKa = 11.84 HH58 pKa = 5.19 GRR60 pKa = 11.84 KK61 pKa = 9.04 QDD63 pKa = 3.48 FQALLSEE70 pKa = 4.42 EE71 pKa = 4.06 LRR73 pKa = 11.84 EE74 pKa = 4.33 IRR76 pKa = 11.84 YY77 pKa = 9.85 DD78 pKa = 3.43 PP79 pKa = 4.4
Molecular weight: 9.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.75
IPC2_protein 8.639
IPC_protein 8.77
Toseland 9.151
ProMoST 9.048
Dawson 9.428
Bjellqvist 9.253
Wikipedia 9.633
Rodwell 9.56
Grimsley 9.502
Solomon 9.604
Lehninger 9.575
Nozaki 9.268
DTASelect 9.194
Thurlkill 9.268
EMBOSS 9.56
Sillero 9.428
Patrickios 4.8
IPC_peptide 9.589
IPC2_peptide 8.039
IPC2.peptide.svr19 7.742
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
3758
79
2097
469.8
54.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.741 ± 0.428
2.076 ± 0.358
6.546 ± 0.803
6.067 ± 0.537
5.003 ± 0.418
5.695 ± 0.564
2.182 ± 0.185
9.5 ± 0.71
7.371 ± 0.553
9.047 ± 0.55
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.501 ± 0.285
5.774 ± 0.466
3.991 ± 0.309
2.954 ± 0.184
4.577 ± 0.347
8.089 ± 0.504
5.535 ± 0.497
4.657 ± 0.413
1.756 ± 0.281
3.938 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here