Amino acid dipepetide frequency for Aquamicrobium phage P14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.567AlaAla: 12.567 ± 1.509
0.649AlaCys: 0.649 ± 0.259
6.162AlaAsp: 6.162 ± 0.648
5.27AlaGlu: 5.27 ± 0.763
3.486AlaPhe: 3.486 ± 0.639
7.54AlaGly: 7.54 ± 1.209
2.027AlaHis: 2.027 ± 0.273
4.297AlaIle: 4.297 ± 0.502
5.189AlaLys: 5.189 ± 0.989
10.621AlaLeu: 10.621 ± 1.244
2.676AlaMet: 2.676 ± 0.314
3.892AlaAsn: 3.892 ± 0.884
2.513AlaPro: 2.513 ± 0.591
4.946AlaGln: 4.946 ± 0.931
6.081AlaArg: 6.081 ± 0.847
5.675AlaSer: 5.675 ± 0.746
5.189AlaThr: 5.189 ± 0.907
6.324AlaVal: 6.324 ± 0.836
1.135AlaTrp: 1.135 ± 0.403
2.838AlaTyr: 2.838 ± 0.564
0.0AlaXaa: 0.0 ± 0.0
Cys
0.649CysAla: 0.649 ± 0.239
0.162CysCys: 0.162 ± 0.095
0.324CysAsp: 0.324 ± 0.138
0.243CysGlu: 0.243 ± 0.131
0.324CysPhe: 0.324 ± 0.163
0.811CysGly: 0.811 ± 0.349
0.486CysHis: 0.486 ± 0.224
0.486CysIle: 0.486 ± 0.258
0.324CysLys: 0.324 ± 0.134
0.811CysLeu: 0.811 ± 0.27
0.081CysMet: 0.081 ± 0.072
0.486CysAsn: 0.486 ± 0.201
0.243CysPro: 0.243 ± 0.148
0.405CysGln: 0.405 ± 0.231
0.162CysArg: 0.162 ± 0.119
0.243CysSer: 0.243 ± 0.153
0.649CysThr: 0.649 ± 0.247
0.892CysVal: 0.892 ± 0.273
0.324CysTrp: 0.324 ± 0.187
0.162CysTyr: 0.162 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
5.594AspAla: 5.594 ± 0.729
0.486AspCys: 0.486 ± 0.177
2.594AspAsp: 2.594 ± 0.561
3.73AspGlu: 3.73 ± 0.493
2.838AspPhe: 2.838 ± 0.454
4.378AspGly: 4.378 ± 0.719
0.73AspHis: 0.73 ± 0.213
3.405AspIle: 3.405 ± 0.422
3.973AspLys: 3.973 ± 0.532
5.513AspLeu: 5.513 ± 0.733
1.297AspMet: 1.297 ± 0.321
1.946AspAsn: 1.946 ± 0.421
3.243AspPro: 3.243 ± 0.432
2.027AspGln: 2.027 ± 0.404
3.648AspArg: 3.648 ± 0.536
3.567AspSer: 3.567 ± 0.419
4.459AspThr: 4.459 ± 0.798
4.54AspVal: 4.54 ± 0.526
0.486AspTrp: 0.486 ± 0.255
2.513AspTyr: 2.513 ± 0.554
0.0AspXaa: 0.0 ± 0.0
Glu
8.189GluAla: 8.189 ± 0.78
0.892GluCys: 0.892 ± 0.316
4.297GluAsp: 4.297 ± 0.674
4.54GluGlu: 4.54 ± 0.524
2.513GluPhe: 2.513 ± 0.452
4.702GluGly: 4.702 ± 0.683
1.622GluHis: 1.622 ± 0.445
2.757GluIle: 2.757 ± 0.434
3.405GluLys: 3.405 ± 0.557
4.459GluLeu: 4.459 ± 0.548
1.378GluMet: 1.378 ± 0.276
1.297GluAsn: 1.297 ± 0.255
1.297GluPro: 1.297 ± 0.316
3.567GluGln: 3.567 ± 0.433
4.459GluArg: 4.459 ± 0.642
3.0GluSer: 3.0 ± 0.521
3.324GluThr: 3.324 ± 0.481
5.675GluVal: 5.675 ± 0.654
1.054GluTrp: 1.054 ± 0.307
2.189GluTyr: 2.189 ± 0.319
0.0GluXaa: 0.0 ± 0.0
Phe
1.784PheAla: 1.784 ± 0.333
0.162PheCys: 0.162 ± 0.111
3.162PheAsp: 3.162 ± 0.406
1.784PheGlu: 1.784 ± 0.312
0.892PhePhe: 0.892 ± 0.263
2.676PheGly: 2.676 ± 0.442
0.811PheHis: 0.811 ± 0.225
1.784PheIle: 1.784 ± 0.398
2.594PheLys: 2.594 ± 0.45
3.73PheLeu: 3.73 ± 0.484
0.649PheMet: 0.649 ± 0.191
1.297PheAsn: 1.297 ± 0.296
1.459PhePro: 1.459 ± 0.434
1.703PheGln: 1.703 ± 0.374
1.784PheArg: 1.784 ± 0.334
2.676PheSer: 2.676 ± 0.45
2.594PheThr: 2.594 ± 0.478
2.351PheVal: 2.351 ± 0.425
0.324PheTrp: 0.324 ± 0.147
0.486PheTyr: 0.486 ± 0.166
0.0PheXaa: 0.0 ± 0.0
Gly
6.243GlyAla: 6.243 ± 0.967
0.73GlyCys: 0.73 ± 0.26
4.621GlyAsp: 4.621 ± 0.642
5.351GlyGlu: 5.351 ± 0.725
3.567GlyPhe: 3.567 ± 0.595
6.81GlyGly: 6.81 ± 0.841
1.459GlyHis: 1.459 ± 0.298
2.757GlyIle: 2.757 ± 0.436
4.135GlyLys: 4.135 ± 0.542
5.432GlyLeu: 5.432 ± 0.627
2.108GlyMet: 2.108 ± 0.421
3.405GlyAsn: 3.405 ± 0.471
2.189GlyPro: 2.189 ± 0.539
3.081GlyGln: 3.081 ± 0.421
5.27GlyArg: 5.27 ± 0.696
3.648GlySer: 3.648 ± 0.499
4.459GlyThr: 4.459 ± 0.606
6.243GlyVal: 6.243 ± 0.714
1.054GlyTrp: 1.054 ± 0.213
2.676GlyTyr: 2.676 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
1.54HisAla: 1.54 ± 0.308
0.162HisCys: 0.162 ± 0.118
1.216HisAsp: 1.216 ± 0.377
1.54HisGlu: 1.54 ± 0.364
0.973HisPhe: 0.973 ± 0.284
1.54HisGly: 1.54 ± 0.338
0.486HisHis: 0.486 ± 0.202
1.054HisIle: 1.054 ± 0.268
0.73HisLys: 0.73 ± 0.245
2.027HisLeu: 2.027 ± 0.506
0.568HisMet: 0.568 ± 0.213
0.973HisAsn: 0.973 ± 0.302
1.54HisPro: 1.54 ± 0.315
0.973HisGln: 0.973 ± 0.256
0.973HisArg: 0.973 ± 0.23
0.892HisSer: 0.892 ± 0.29
1.459HisThr: 1.459 ± 0.249
1.622HisVal: 1.622 ± 0.382
0.162HisTrp: 0.162 ± 0.107
0.162HisTyr: 0.162 ± 0.104
0.0HisXaa: 0.0 ± 0.0
Ile
4.297IleAla: 4.297 ± 0.544
0.162IleCys: 0.162 ± 0.119
2.513IleAsp: 2.513 ± 0.47
2.676IleGlu: 2.676 ± 0.438
0.811IlePhe: 0.811 ± 0.187
2.513IleGly: 2.513 ± 0.403
1.622IleHis: 1.622 ± 0.428
2.351IleIle: 2.351 ± 0.458
3.0IleLys: 3.0 ± 0.605
4.297IleLeu: 4.297 ± 0.598
0.892IleMet: 0.892 ± 0.269
2.757IleAsn: 2.757 ± 0.473
2.027IlePro: 2.027 ± 0.447
2.189IleGln: 2.189 ± 0.47
3.162IleArg: 3.162 ± 0.422
3.081IleSer: 3.081 ± 0.425
2.757IleThr: 2.757 ± 0.585
3.162IleVal: 3.162 ± 0.491
0.243IleTrp: 0.243 ± 0.119
1.378IleTyr: 1.378 ± 0.31
0.0IleXaa: 0.0 ± 0.0
Lys
7.297LysAla: 7.297 ± 0.81
0.486LysCys: 0.486 ± 0.181
3.081LysAsp: 3.081 ± 0.622
3.405LysGlu: 3.405 ± 0.648
1.946LysPhe: 1.946 ± 0.339
3.324LysGly: 3.324 ± 0.706
0.892LysHis: 0.892 ± 0.204
1.297LysIle: 1.297 ± 0.437
2.351LysLys: 2.351 ± 0.452
5.513LysLeu: 5.513 ± 0.808
0.973LysMet: 0.973 ± 0.254
2.594LysAsn: 2.594 ± 0.575
2.189LysPro: 2.189 ± 0.324
1.865LysGln: 1.865 ± 0.58
3.567LysArg: 3.567 ± 0.642
2.432LysSer: 2.432 ± 0.38
1.622LysThr: 1.622 ± 0.299
3.973LysVal: 3.973 ± 0.608
1.135LysTrp: 1.135 ± 0.311
1.622LysTyr: 1.622 ± 0.373
0.0LysXaa: 0.0 ± 0.0
Leu
8.918LeuAla: 8.918 ± 0.861
1.135LeuCys: 1.135 ± 0.391
5.838LeuAsp: 5.838 ± 0.576
5.838LeuGlu: 5.838 ± 0.737
2.27LeuPhe: 2.27 ± 0.437
7.054LeuGly: 7.054 ± 1.191
1.297LeuHis: 1.297 ± 0.347
3.486LeuIle: 3.486 ± 0.441
4.621LeuLys: 4.621 ± 0.574
7.135LeuLeu: 7.135 ± 1.064
1.865LeuMet: 1.865 ± 0.473
3.486LeuAsn: 3.486 ± 0.509
4.297LeuPro: 4.297 ± 0.398
3.324LeuGln: 3.324 ± 0.58
5.189LeuArg: 5.189 ± 0.447
5.27LeuSer: 5.27 ± 0.663
6.162LeuThr: 6.162 ± 0.59
6.162LeuVal: 6.162 ± 0.779
1.135LeuTrp: 1.135 ± 0.328
2.919LeuTyr: 2.919 ± 0.556
0.0LeuXaa: 0.0 ± 0.0
Met
2.189MetAla: 2.189 ± 0.464
0.081MetCys: 0.081 ± 0.081
1.703MetAsp: 1.703 ± 0.342
1.135MetGlu: 1.135 ± 0.451
0.243MetPhe: 0.243 ± 0.118
1.784MetGly: 1.784 ± 0.377
0.568MetHis: 0.568 ± 0.19
1.703MetIle: 1.703 ± 0.362
0.73MetLys: 0.73 ± 0.252
2.189MetLeu: 2.189 ± 0.362
0.162MetMet: 0.162 ± 0.09
0.892MetAsn: 0.892 ± 0.259
1.054MetPro: 1.054 ± 0.296
1.459MetGln: 1.459 ± 0.405
1.378MetArg: 1.378 ± 0.364
2.108MetSer: 2.108 ± 0.381
1.703MetThr: 1.703 ± 0.283
1.622MetVal: 1.622 ± 0.374
0.162MetTrp: 0.162 ± 0.114
1.135MetTyr: 1.135 ± 0.285
0.0MetXaa: 0.0 ± 0.0
Asn
2.432AsnAla: 2.432 ± 0.424
0.081AsnCys: 0.081 ± 0.084
1.378AsnAsp: 1.378 ± 0.254
2.513AsnGlu: 2.513 ± 0.462
1.297AsnPhe: 1.297 ± 0.33
3.567AsnGly: 3.567 ± 0.433
0.73AsnHis: 0.73 ± 0.221
1.946AsnIle: 1.946 ± 0.422
2.189AsnLys: 2.189 ± 0.467
3.73AsnLeu: 3.73 ± 0.458
0.973AsnMet: 0.973 ± 0.251
1.54AsnAsn: 1.54 ± 0.361
2.513AsnPro: 2.513 ± 0.625
2.513AsnGln: 2.513 ± 0.377
2.351AsnArg: 2.351 ± 0.429
2.189AsnSer: 2.189 ± 0.495
2.351AsnThr: 2.351 ± 0.45
1.703AsnVal: 1.703 ± 0.348
0.892AsnTrp: 0.892 ± 0.224
1.297AsnTyr: 1.297 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
3.73ProAla: 3.73 ± 0.857
0.405ProCys: 0.405 ± 0.182
3.567ProAsp: 3.567 ± 0.489
3.162ProGlu: 3.162 ± 0.452
1.378ProPhe: 1.378 ± 0.337
3.892ProGly: 3.892 ± 0.454
0.649ProHis: 0.649 ± 0.238
1.703ProIle: 1.703 ± 0.301
1.946ProLys: 1.946 ± 0.325
2.919ProLeu: 2.919 ± 0.521
1.216ProMet: 1.216 ± 0.372
1.54ProAsn: 1.54 ± 0.303
1.865ProPro: 1.865 ± 0.478
1.378ProGln: 1.378 ± 0.393
1.297ProArg: 1.297 ± 0.362
2.594ProSer: 2.594 ± 0.435
3.081ProThr: 3.081 ± 0.672
3.973ProVal: 3.973 ± 0.556
0.486ProTrp: 0.486 ± 0.168
1.622ProTyr: 1.622 ± 0.348
0.0ProXaa: 0.0 ± 0.0
Gln
5.189GlnAla: 5.189 ± 0.804
0.568GlnCys: 0.568 ± 0.237
2.757GlnAsp: 2.757 ± 0.396
4.135GlnGlu: 4.135 ± 0.556
1.297GlnPhe: 1.297 ± 0.393
3.162GlnGly: 3.162 ± 0.579
0.568GlnHis: 0.568 ± 0.266
1.622GlnIle: 1.622 ± 0.354
2.108GlnLys: 2.108 ± 0.425
3.162GlnLeu: 3.162 ± 0.426
1.135GlnMet: 1.135 ± 0.395
1.378GlnAsn: 1.378 ± 0.375
0.811GlnPro: 0.811 ± 0.248
2.594GlnGln: 2.594 ± 0.745
3.567GlnArg: 3.567 ± 0.554
2.594GlnSer: 2.594 ± 0.595
2.432GlnThr: 2.432 ± 0.452
3.486GlnVal: 3.486 ± 0.44
0.73GlnTrp: 0.73 ± 0.2
1.622GlnTyr: 1.622 ± 0.335
0.0GlnXaa: 0.0 ± 0.0
Arg
5.432ArgAla: 5.432 ± 1.004
0.568ArgCys: 0.568 ± 0.217
3.73ArgAsp: 3.73 ± 0.443
4.297ArgGlu: 4.297 ± 0.664
2.27ArgPhe: 2.27 ± 0.357
3.973ArgGly: 3.973 ± 0.523
1.054ArgHis: 1.054 ± 0.265
3.324ArgIle: 3.324 ± 0.539
3.324ArgLys: 3.324 ± 0.639
5.838ArgLeu: 5.838 ± 0.736
1.703ArgMet: 1.703 ± 0.422
2.432ArgAsn: 2.432 ± 0.636
2.108ArgPro: 2.108 ± 0.412
2.27ArgGln: 2.27 ± 0.465
3.648ArgArg: 3.648 ± 0.543
3.486ArgSer: 3.486 ± 0.388
3.243ArgThr: 3.243 ± 0.522
4.702ArgVal: 4.702 ± 0.588
0.973ArgTrp: 0.973 ± 0.272
3.0ArgTyr: 3.0 ± 0.501
0.0ArgXaa: 0.0 ± 0.0
Ser
4.865SerAla: 4.865 ± 0.793
0.486SerCys: 0.486 ± 0.239
3.567SerAsp: 3.567 ± 0.546
3.486SerGlu: 3.486 ± 0.598
2.108SerPhe: 2.108 ± 0.412
4.459SerGly: 4.459 ± 0.92
1.378SerHis: 1.378 ± 0.325
2.919SerIle: 2.919 ± 0.467
2.594SerLys: 2.594 ± 0.507
5.108SerLeu: 5.108 ± 0.455
1.703SerMet: 1.703 ± 0.372
2.108SerAsn: 2.108 ± 0.424
3.081SerPro: 3.081 ± 0.518
3.567SerGln: 3.567 ± 0.651
4.702SerArg: 4.702 ± 0.481
3.567SerSer: 3.567 ± 0.522
4.135SerThr: 4.135 ± 0.793
3.486SerVal: 3.486 ± 0.623
1.054SerTrp: 1.054 ± 0.296
1.459SerTyr: 1.459 ± 0.292
0.0SerXaa: 0.0 ± 0.0
Thr
6.0ThrAla: 6.0 ± 1.295
0.324ThrCys: 0.324 ± 0.153
3.405ThrAsp: 3.405 ± 0.524
3.324ThrGlu: 3.324 ± 0.578
2.351ThrPhe: 2.351 ± 0.438
4.297ThrGly: 4.297 ± 0.528
0.973ThrHis: 0.973 ± 0.241
3.324ThrIle: 3.324 ± 0.57
3.162ThrLys: 3.162 ± 0.466
5.189ThrLeu: 5.189 ± 0.71
1.54ThrMet: 1.54 ± 0.37
2.27ThrAsn: 2.27 ± 0.334
4.216ThrPro: 4.216 ± 0.867
2.108ThrGln: 2.108 ± 0.595
2.189ThrArg: 2.189 ± 0.293
4.784ThrSer: 4.784 ± 0.766
3.648ThrThr: 3.648 ± 0.636
4.378ThrVal: 4.378 ± 0.626
0.73ThrTrp: 0.73 ± 0.216
2.027ThrTyr: 2.027 ± 0.432
0.0ThrXaa: 0.0 ± 0.0
Val
7.702ValAla: 7.702 ± 0.857
0.243ValCys: 0.243 ± 0.139
4.378ValAsp: 4.378 ± 0.566
5.027ValGlu: 5.027 ± 0.704
2.432ValPhe: 2.432 ± 0.353
5.432ValGly: 5.432 ± 0.667
2.351ValHis: 2.351 ± 0.357
2.838ValIle: 2.838 ± 0.615
3.405ValLys: 3.405 ± 0.777
5.27ValLeu: 5.27 ± 0.561
1.784ValMet: 1.784 ± 0.352
2.594ValAsn: 2.594 ± 0.454
3.648ValPro: 3.648 ± 0.601
3.243ValGln: 3.243 ± 0.62
4.865ValArg: 4.865 ± 0.677
4.784ValSer: 4.784 ± 0.692
4.54ValThr: 4.54 ± 0.563
5.513ValVal: 5.513 ± 0.724
1.135ValTrp: 1.135 ± 0.304
2.513ValTyr: 2.513 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
1.54TrpAla: 1.54 ± 0.342
0.243TrpCys: 0.243 ± 0.154
0.811TrpAsp: 0.811 ± 0.309
0.973TrpGlu: 0.973 ± 0.231
0.811TrpPhe: 0.811 ± 0.295
0.73TrpGly: 0.73 ± 0.256
0.243TrpHis: 0.243 ± 0.117
0.486TrpIle: 0.486 ± 0.172
0.811TrpLys: 0.811 ± 0.267
1.216TrpLeu: 1.216 ± 0.325
0.486TrpMet: 0.486 ± 0.192
0.324TrpAsn: 0.324 ± 0.17
0.568TrpPro: 0.568 ± 0.217
0.568TrpGln: 0.568 ± 0.217
0.405TrpArg: 0.405 ± 0.154
0.892TrpSer: 0.892 ± 0.295
0.73TrpThr: 0.73 ± 0.235
1.459TrpVal: 1.459 ± 0.463
0.324TrpTrp: 0.324 ± 0.159
0.405TrpTyr: 0.405 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.919TyrAla: 2.919 ± 0.483
0.243TyrCys: 0.243 ± 0.142
1.865TyrAsp: 1.865 ± 0.443
2.189TyrGlu: 2.189 ± 0.441
0.892TyrPhe: 0.892 ± 0.423
2.594TyrGly: 2.594 ± 0.421
0.649TyrHis: 0.649 ± 0.224
2.351TyrIle: 2.351 ± 0.421
0.973TyrLys: 0.973 ± 0.258
3.243TyrLeu: 3.243 ± 0.341
0.73TyrMet: 0.73 ± 0.261
0.973TyrAsn: 0.973 ± 0.246
1.54TyrPro: 1.54 ± 0.445
1.135TyrGln: 1.135 ± 0.339
2.676TyrArg: 2.676 ± 0.366
2.432TyrSer: 2.432 ± 0.301
1.703TyrThr: 1.703 ± 0.429
2.432TyrVal: 2.432 ± 0.409
0.486TyrTrp: 0.486 ± 0.182
0.973TyrTyr: 0.973 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12335 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski