Aeromonas phage 4_4572

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lahexavirus; Aeromonas virus 4-4572

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NC85|A0A5B9NC85_9CAUD Uncharacterized protein OS=Aeromonas phage 4_4572 OX=2588517 GN=44572_073 PE=4 SV=1
MM1 pKa = 6.99VAILFTFALLIALAVVIYY19 pKa = 9.03TDD21 pKa = 3.64SVIVKK26 pKa = 9.92VMEE29 pKa = 4.5NYY31 pKa = 10.68DD32 pKa = 3.71EE33 pKa = 5.71GGDD36 pKa = 3.51IHH38 pKa = 7.63QDD40 pKa = 3.85LIDD43 pKa = 4.71HH44 pKa = 7.19IIDD47 pKa = 3.43HH48 pKa = 6.3MANNDD53 pKa = 3.15

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N4D0|A0A5B9N4D0_9CAUD Uncharacterized protein OS=Aeromonas phage 4_4572 OX=2588517 GN=44572_049 PE=4 SV=1
MM1 pKa = 7.92PSNEE5 pKa = 4.08NYY7 pKa = 9.66VRR9 pKa = 11.84DD10 pKa = 3.84YY11 pKa = 10.41EE12 pKa = 4.34QEE14 pKa = 3.7RR15 pKa = 11.84KK16 pKa = 7.31TAIKK20 pKa = 10.34RR21 pKa = 11.84GEE23 pKa = 4.08TGVGSKK29 pKa = 10.65SKK31 pKa = 10.13DD32 pKa = 3.44AKK34 pKa = 8.91RR35 pKa = 11.84HH36 pKa = 4.68RR37 pKa = 11.84ARR39 pKa = 11.84RR40 pKa = 11.84IVEE43 pKa = 3.74KK44 pKa = 10.73RR45 pKa = 11.84LGRR48 pKa = 11.84KK49 pKa = 8.53LRR51 pKa = 11.84PDD53 pKa = 3.06EE54 pKa = 4.91HH55 pKa = 7.87VDD57 pKa = 3.01HH58 pKa = 7.11KK59 pKa = 11.43KK60 pKa = 9.92PLKK63 pKa = 10.46EE64 pKa = 4.19GGSNHH69 pKa = 6.14SSNLAVRR76 pKa = 11.84PASSNTSAGGKK87 pKa = 8.52MGSKK91 pKa = 9.97AGKK94 pKa = 9.47AAGARR99 pKa = 11.84KK100 pKa = 8.57GHH102 pKa = 6.84KK103 pKa = 9.79SRR105 pKa = 11.84LPVKK109 pKa = 10.41KK110 pKa = 10.14PSS112 pKa = 3.16

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

154

0

154

30176

30

1446

195.9

22.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.823 ± 0.458

1.186 ± 0.119

6.214 ± 0.204

7.264 ± 0.286

4.427 ± 0.212

6.943 ± 0.238

1.852 ± 0.13

5.776 ± 0.183

7.131 ± 0.286

7.675 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.012 ± 0.129

5.064 ± 0.192

3.695 ± 0.153

3.503 ± 0.185

4.765 ± 0.237

6.721 ± 0.183

5.455 ± 0.232

7.234 ± 0.215

1.713 ± 0.111

3.546 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski