Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1482 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9HIP0|RL37A_THEAC 50S ribosomal protein L37Ae OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=rpl37ae PE=3 SV=1
MM1 pKa = 6.7 NTEE4 pKa = 3.86 HH5 pKa = 6.13 TVSVFILIFLFIGSYY20 pKa = 9.56 SAPGSISTIHH30 pKa = 5.82 QPAYY34 pKa = 9.24 VQNTKK39 pKa = 9.62 MSANNTSEE47 pKa = 4.21 TIDD50 pKa = 4.78 LINDD54 pKa = 3.62 SVISGNFMDD63 pKa = 4.45 TGNAIVPTSGAYY75 pKa = 10.02 ASDD78 pKa = 3.62 CGCYY82 pKa = 9.53 FVANYY87 pKa = 7.49 FTDD90 pKa = 3.24 NVYY93 pKa = 10.9 VINATTYY100 pKa = 10.26 RR101 pKa = 11.84 IANVMPVGSGPISVMYY117 pKa = 10.61 VEE119 pKa = 5.26 SNGLVYY125 pKa = 10.49 VANQNSGDD133 pKa = 3.22 ISVINTHH140 pKa = 6.13 LMSVIDD146 pKa = 4.33 TIGVGSGPQSIAYY159 pKa = 9.07 DD160 pKa = 3.74 PQIDD164 pKa = 3.83 EE165 pKa = 4.43 VFIANSYY172 pKa = 8.98 SGSVTVISGSNSVVANISVAGASGVAYY199 pKa = 9.98 SQYY202 pKa = 10.91 NGYY205 pKa = 10.05 IYY207 pKa = 10.54 VSQYY211 pKa = 8.14 GHH213 pKa = 6.16 GMVSVINPLTLSTVGTFEE231 pKa = 4.94 AGSSPSSLAIDD242 pKa = 3.68 NLNGKK247 pKa = 8.22 VFIDD251 pKa = 3.7 NQGSDD256 pKa = 4.27 TITVTGPNGTYY267 pKa = 9.94 IRR269 pKa = 11.84 TIPVGSDD276 pKa = 2.9 PLQVMFCNYY285 pKa = 9.62 SDD287 pKa = 3.8 QVYY290 pKa = 8.85 TSNFDD295 pKa = 3.45 SDD297 pKa = 3.92 TVTAINAINFSSITNISVGLNPYY320 pKa = 10.0 GLFMGNGRR328 pKa = 11.84 NEE330 pKa = 3.48 IAVANEE336 pKa = 3.62 YY337 pKa = 9.89 TNNISFINTSTDD349 pKa = 2.81 EE350 pKa = 4.29 VYY352 pKa = 11.11 YY353 pKa = 10.85 SLTIGSGPQGLAYY366 pKa = 10.48 DD367 pKa = 4.21 PVNSYY372 pKa = 11.3 LYY374 pKa = 9.0 VTNAYY379 pKa = 10.87 SNFVYY384 pKa = 10.78 VLDD387 pKa = 4.23 KK388 pKa = 10.87 NYY390 pKa = 11.04 SVIAKK395 pKa = 9.49 VYY397 pKa = 9.72 VGWGPSGIAYY407 pKa = 9.78 DD408 pKa = 3.98 PYY410 pKa = 11.1 NNYY413 pKa = 10.05 VYY415 pKa = 10.57 VANYY419 pKa = 7.82 DD420 pKa = 3.56 TDD422 pKa = 3.78 NVSVLDD428 pKa = 4.0 TQTQTVLRR436 pKa = 11.84 NIAVGEE442 pKa = 4.5 GPAGIVVNPSNGYY455 pKa = 9.58 VYY457 pKa = 10.56 SINQLSDD464 pKa = 3.51 DD465 pKa = 3.81 VSVINPSNNSVISSIITGSSPFNGVYY491 pKa = 10.33 DD492 pKa = 3.67 QANGFIYY499 pKa = 10.65 VGNFGSNNISVINPDD514 pKa = 3.64 SEE516 pKa = 4.66 SVVYY520 pKa = 8.79 TIQVGSGPQDD530 pKa = 3.36 LSYY533 pKa = 11.2 DD534 pKa = 3.67 PEE536 pKa = 4.84 NGYY539 pKa = 10.3 IYY541 pKa = 10.37 VINFNSDD548 pKa = 2.88 SVSIINGKK556 pKa = 8.65 EE557 pKa = 3.79 RR558 pKa = 11.84 VLSTINTGSGPEE570 pKa = 4.36 GICYY574 pKa = 10.24 DD575 pKa = 3.95 PYY577 pKa = 11.72 DD578 pKa = 3.7 NSLYY582 pKa = 10.67 VSNSGSNDD590 pKa = 2.97 VYY592 pKa = 11.23 VINATTEE599 pKa = 4.02 GVEE602 pKa = 4.21 AIIPVGIYY610 pKa = 9.46 PFRR613 pKa = 11.84 DD614 pKa = 3.42 CVDD617 pKa = 3.67 PANGLVFVANTNSGTVSIIEE637 pKa = 4.22 PMSPTAGTVISPHH650 pKa = 5.05 VQNDD654 pKa = 3.22 ALFFILAILVCALAITGYY672 pKa = 10.21 IATIKK677 pKa = 10.09 HH678 pKa = 5.56 KK679 pKa = 10.64 KK680 pKa = 8.65
Molecular weight: 72.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.89
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.935
Patrickios 0.006
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|Q9HM23|Q9HM23_THEAC Phosphate permease related protein OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=Ta0048 PE=4 SV=1
MM1 pKa = 7.4 SRR3 pKa = 11.84 NKK5 pKa = 10.27 EE6 pKa = 3.86 LGRR9 pKa = 11.84 KK10 pKa = 7.91 IRR12 pKa = 11.84 LMKK15 pKa = 10.23 KK16 pKa = 8.44 IKK18 pKa = 9.27 QNRR21 pKa = 11.84 RR22 pKa = 11.84 VPGWVMMRR30 pKa = 11.84 TARR33 pKa = 11.84 KK34 pKa = 7.94 VTQNPLRR41 pKa = 11.84 RR42 pKa = 11.84 NWRR45 pKa = 11.84 RR46 pKa = 11.84 GSLKK50 pKa = 10.12 II51 pKa = 4.26
Molecular weight: 6.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.426
IPC2_protein 10.921
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1482
0
1482
453232
45
2081
305.8
34.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.96 ± 0.055
0.601 ± 0.018
5.751 ± 0.061
5.984 ± 0.079
4.701 ± 0.056
7.251 ± 0.054
1.646 ± 0.02
9.021 ± 0.055
5.641 ± 0.068
8.384 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.195 ± 0.032
4.251 ± 0.053
3.963 ± 0.039
2.157 ± 0.031
5.499 ± 0.07
7.568 ± 0.061
4.779 ± 0.05
7.157 ± 0.052
0.854 ± 0.023
4.635 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here