Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales; Thermoplasmataceae; Thermoplasma; Thermoplasma acidophilum

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1482 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9HIP0|RL37A_THEAC 50S ribosomal protein L37Ae OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=rpl37ae PE=3 SV=1
MM1 pKa = 6.7NTEE4 pKa = 3.86HH5 pKa = 6.13TVSVFILIFLFIGSYY20 pKa = 9.56SAPGSISTIHH30 pKa = 5.82QPAYY34 pKa = 9.24VQNTKK39 pKa = 9.62MSANNTSEE47 pKa = 4.21TIDD50 pKa = 4.78LINDD54 pKa = 3.62SVISGNFMDD63 pKa = 4.45TGNAIVPTSGAYY75 pKa = 10.02ASDD78 pKa = 3.62CGCYY82 pKa = 9.53FVANYY87 pKa = 7.49FTDD90 pKa = 3.24NVYY93 pKa = 10.9VINATTYY100 pKa = 10.26RR101 pKa = 11.84IANVMPVGSGPISVMYY117 pKa = 10.61VEE119 pKa = 5.26SNGLVYY125 pKa = 10.49VANQNSGDD133 pKa = 3.22ISVINTHH140 pKa = 6.13LMSVIDD146 pKa = 4.33TIGVGSGPQSIAYY159 pKa = 9.07DD160 pKa = 3.74PQIDD164 pKa = 3.83EE165 pKa = 4.43VFIANSYY172 pKa = 8.98SGSVTVISGSNSVVANISVAGASGVAYY199 pKa = 9.98SQYY202 pKa = 10.91NGYY205 pKa = 10.05IYY207 pKa = 10.54VSQYY211 pKa = 8.14GHH213 pKa = 6.16GMVSVINPLTLSTVGTFEE231 pKa = 4.94AGSSPSSLAIDD242 pKa = 3.68NLNGKK247 pKa = 8.22VFIDD251 pKa = 3.7NQGSDD256 pKa = 4.27TITVTGPNGTYY267 pKa = 9.94IRR269 pKa = 11.84TIPVGSDD276 pKa = 2.9PLQVMFCNYY285 pKa = 9.62SDD287 pKa = 3.8QVYY290 pKa = 8.85TSNFDD295 pKa = 3.45SDD297 pKa = 3.92TVTAINAINFSSITNISVGLNPYY320 pKa = 10.0GLFMGNGRR328 pKa = 11.84NEE330 pKa = 3.48IAVANEE336 pKa = 3.62YY337 pKa = 9.89TNNISFINTSTDD349 pKa = 2.81EE350 pKa = 4.29VYY352 pKa = 11.11YY353 pKa = 10.85SLTIGSGPQGLAYY366 pKa = 10.48DD367 pKa = 4.21PVNSYY372 pKa = 11.3LYY374 pKa = 9.0VTNAYY379 pKa = 10.87SNFVYY384 pKa = 10.78VLDD387 pKa = 4.23KK388 pKa = 10.87NYY390 pKa = 11.04SVIAKK395 pKa = 9.49VYY397 pKa = 9.72VGWGPSGIAYY407 pKa = 9.78DD408 pKa = 3.98PYY410 pKa = 11.1NNYY413 pKa = 10.05VYY415 pKa = 10.57VANYY419 pKa = 7.82DD420 pKa = 3.56TDD422 pKa = 3.78NVSVLDD428 pKa = 4.0TQTQTVLRR436 pKa = 11.84NIAVGEE442 pKa = 4.5GPAGIVVNPSNGYY455 pKa = 9.58VYY457 pKa = 10.56SINQLSDD464 pKa = 3.51DD465 pKa = 3.81VSVINPSNNSVISSIITGSSPFNGVYY491 pKa = 10.33DD492 pKa = 3.67QANGFIYY499 pKa = 10.65VGNFGSNNISVINPDD514 pKa = 3.64SEE516 pKa = 4.66SVVYY520 pKa = 8.79TIQVGSGPQDD530 pKa = 3.36LSYY533 pKa = 11.2DD534 pKa = 3.67PEE536 pKa = 4.84NGYY539 pKa = 10.3IYY541 pKa = 10.37VINFNSDD548 pKa = 2.88SVSIINGKK556 pKa = 8.65EE557 pKa = 3.79RR558 pKa = 11.84VLSTINTGSGPEE570 pKa = 4.36GICYY574 pKa = 10.24DD575 pKa = 3.95PYY577 pKa = 11.72DD578 pKa = 3.7NSLYY582 pKa = 10.67VSNSGSNDD590 pKa = 2.97VYY592 pKa = 11.23VINATTEE599 pKa = 4.02GVEE602 pKa = 4.21AIIPVGIYY610 pKa = 9.46PFRR613 pKa = 11.84DD614 pKa = 3.42CVDD617 pKa = 3.67PANGLVFVANTNSGTVSIIEE637 pKa = 4.22PMSPTAGTVISPHH650 pKa = 5.05VQNDD654 pKa = 3.22ALFFILAILVCALAITGYY672 pKa = 10.21IATIKK677 pKa = 10.09HH678 pKa = 5.56KK679 pKa = 10.64KK680 pKa = 8.65

Molecular weight: 72.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9HM23|Q9HM23_THEAC Phosphate permease related protein OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=Ta0048 PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84NKK5 pKa = 10.27EE6 pKa = 3.86LGRR9 pKa = 11.84KK10 pKa = 7.91IRR12 pKa = 11.84LMKK15 pKa = 10.23KK16 pKa = 8.44IKK18 pKa = 9.27QNRR21 pKa = 11.84RR22 pKa = 11.84VPGWVMMRR30 pKa = 11.84TARR33 pKa = 11.84KK34 pKa = 7.94VTQNPLRR41 pKa = 11.84RR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84GSLKK50 pKa = 10.12II51 pKa = 4.26

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1482

0

1482

453232

45

2081

305.8

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.96 ± 0.055

0.601 ± 0.018

5.751 ± 0.061

5.984 ± 0.079

4.701 ± 0.056

7.251 ± 0.054

1.646 ± 0.02

9.021 ± 0.055

5.641 ± 0.068

8.384 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.195 ± 0.032

4.251 ± 0.053

3.963 ± 0.039

2.157 ± 0.031

5.499 ± 0.07

7.568 ± 0.061

4.779 ± 0.05

7.157 ± 0.052

0.854 ± 0.023

4.635 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski