Amino acid dipepetide frequency for Enterobacter phage Enc34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.842AlaAla: 10.842 ± 0.882
0.684AlaCys: 0.684 ± 0.194
4.947AlaAsp: 4.947 ± 0.525
5.631AlaGlu: 5.631 ± 0.683
3.368AlaPhe: 3.368 ± 0.393
7.473AlaGly: 7.473 ± 0.65
1.105AlaHis: 1.105 ± 0.259
5.052AlaIle: 5.052 ± 0.514
4.842AlaLys: 4.842 ± 0.581
8.052AlaLeu: 8.052 ± 0.628
3.052AlaMet: 3.052 ± 0.49
3.474AlaAsn: 3.474 ± 0.479
3.737AlaPro: 3.737 ± 0.438
3.684AlaGln: 3.684 ± 0.487
5.052AlaArg: 5.052 ± 0.679
5.0AlaSer: 5.0 ± 0.519
5.737AlaThr: 5.737 ± 0.588
5.737AlaVal: 5.737 ± 0.683
1.105AlaTrp: 1.105 ± 0.262
2.737AlaTyr: 2.737 ± 0.34
0.0AlaXaa: 0.0 ± 0.0
Cys
0.895CysAla: 0.895 ± 0.243
0.211CysCys: 0.211 ± 0.11
1.0CysAsp: 1.0 ± 0.302
0.579CysGlu: 0.579 ± 0.156
0.579CysPhe: 0.579 ± 0.171
1.158CysGly: 1.158 ± 0.275
0.263CysHis: 0.263 ± 0.1
0.579CysIle: 0.579 ± 0.183
0.421CysLys: 0.421 ± 0.148
0.579CysLeu: 0.579 ± 0.179
0.263CysMet: 0.263 ± 0.105
0.474CysAsn: 0.474 ± 0.175
0.632CysPro: 0.632 ± 0.201
0.316CysGln: 0.316 ± 0.126
0.632CysArg: 0.632 ± 0.209
0.474CysSer: 0.474 ± 0.182
0.737CysThr: 0.737 ± 0.281
0.474CysVal: 0.474 ± 0.206
0.158CysTrp: 0.158 ± 0.083
0.737CysTyr: 0.737 ± 0.187
0.0CysXaa: 0.0 ± 0.0
Asp
6.21AspAla: 6.21 ± 0.55
0.947AspCys: 0.947 ± 0.226
4.631AspAsp: 4.631 ± 0.453
4.789AspGlu: 4.789 ± 0.707
2.421AspPhe: 2.421 ± 0.323
5.263AspGly: 5.263 ± 0.511
1.316AspHis: 1.316 ± 0.245
3.316AspIle: 3.316 ± 0.461
2.842AspLys: 2.842 ± 0.369
4.526AspLeu: 4.526 ± 0.5
2.526AspMet: 2.526 ± 0.372
2.263AspAsn: 2.263 ± 0.457
3.947AspPro: 3.947 ± 0.497
1.947AspGln: 1.947 ± 0.342
2.842AspArg: 2.842 ± 0.403
2.631AspSer: 2.631 ± 0.356
3.842AspThr: 3.842 ± 0.439
5.21AspVal: 5.21 ± 0.461
1.526AspTrp: 1.526 ± 0.29
2.421AspTyr: 2.421 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
5.158GluAla: 5.158 ± 0.681
0.579GluCys: 0.579 ± 0.168
4.052GluAsp: 4.052 ± 0.522
3.684GluGlu: 3.684 ± 0.46
2.21GluPhe: 2.21 ± 0.305
3.474GluGly: 3.474 ± 0.483
0.947GluHis: 0.947 ± 0.315
3.368GluIle: 3.368 ± 0.404
2.789GluLys: 2.789 ± 0.487
4.316GluLeu: 4.316 ± 0.442
1.842GluMet: 1.842 ± 0.326
3.0GluAsn: 3.0 ± 0.44
2.631GluPro: 2.631 ± 0.549
2.474GluGln: 2.474 ± 0.371
3.052GluArg: 3.052 ± 0.451
3.474GluSer: 3.474 ± 0.343
3.105GluThr: 3.105 ± 0.411
4.631GluVal: 4.631 ± 0.556
1.526GluTrp: 1.526 ± 0.276
2.21GluTyr: 2.21 ± 0.286
0.0GluXaa: 0.0 ± 0.0
Phe
3.0PheAla: 3.0 ± 0.387
0.737PheCys: 0.737 ± 0.186
3.526PheAsp: 3.526 ± 0.517
2.21PheGlu: 2.21 ± 0.3
1.158PhePhe: 1.158 ± 0.211
2.789PheGly: 2.789 ± 0.392
0.579PheHis: 0.579 ± 0.149
1.895PheIle: 1.895 ± 0.286
2.737PheLys: 2.737 ± 0.321
2.579PheLeu: 2.579 ± 0.361
0.895PheMet: 0.895 ± 0.177
1.789PheAsn: 1.789 ± 0.286
1.526PhePro: 1.526 ± 0.298
0.684PheGln: 0.684 ± 0.235
1.737PheArg: 1.737 ± 0.353
1.842PheSer: 1.842 ± 0.352
2.316PheThr: 2.316 ± 0.378
2.263PheVal: 2.263 ± 0.347
0.632PheTrp: 0.632 ± 0.2
1.0PheTyr: 1.0 ± 0.227
0.0PheXaa: 0.0 ± 0.0
Gly
5.684GlyAla: 5.684 ± 0.536
1.053GlyCys: 1.053 ± 0.302
3.789GlyAsp: 3.789 ± 0.53
4.526GlyGlu: 4.526 ± 0.443
2.789GlyPhe: 2.789 ± 0.46
6.526GlyGly: 6.526 ± 0.989
0.789GlyHis: 0.789 ± 0.227
3.579GlyIle: 3.579 ± 0.388
4.947GlyLys: 4.947 ± 0.495
5.631GlyLeu: 5.631 ± 0.632
2.947GlyMet: 2.947 ± 0.455
3.052GlyAsn: 3.052 ± 0.422
1.789GlyPro: 1.789 ± 0.307
2.684GlyGln: 2.684 ± 0.341
3.842GlyArg: 3.842 ± 0.485
3.737GlySer: 3.737 ± 0.399
4.473GlyThr: 4.473 ± 0.636
5.842GlyVal: 5.842 ± 0.647
1.526GlyTrp: 1.526 ± 0.27
3.526GlyTyr: 3.526 ± 0.494
0.0GlyXaa: 0.0 ± 0.0
His
1.105HisAla: 1.105 ± 0.236
0.105HisCys: 0.105 ± 0.075
1.368HisAsp: 1.368 ± 0.26
0.895HisGlu: 0.895 ± 0.198
0.684HisPhe: 0.684 ± 0.202
1.0HisGly: 1.0 ± 0.247
0.316HisHis: 0.316 ± 0.145
1.158HisIle: 1.158 ± 0.241
0.632HisLys: 0.632 ± 0.163
1.579HisLeu: 1.579 ± 0.285
0.474HisMet: 0.474 ± 0.145
0.632HisAsn: 0.632 ± 0.182
0.632HisPro: 0.632 ± 0.201
0.421HisGln: 0.421 ± 0.167
1.053HisArg: 1.053 ± 0.211
0.737HisSer: 0.737 ± 0.197
0.947HisThr: 0.947 ± 0.251
1.316HisVal: 1.316 ± 0.332
0.158HisTrp: 0.158 ± 0.099
0.789HisTyr: 0.789 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
5.21IleAla: 5.21 ± 0.511
0.474IleCys: 0.474 ± 0.144
3.631IleAsp: 3.631 ± 0.444
3.684IleGlu: 3.684 ± 0.374
1.474IlePhe: 1.474 ± 0.261
4.158IleGly: 4.158 ± 0.588
1.053IleHis: 1.053 ± 0.227
3.0IleIle: 3.0 ± 0.522
3.316IleLys: 3.316 ± 0.445
3.789IleLeu: 3.789 ± 0.454
1.105IleMet: 1.105 ± 0.236
3.316IleAsn: 3.316 ± 0.488
2.21IlePro: 2.21 ± 0.266
1.579IleGln: 1.579 ± 0.263
3.684IleArg: 3.684 ± 0.582
3.947IleSer: 3.947 ± 0.5
4.526IleThr: 4.526 ± 0.543
3.789IleVal: 3.789 ± 0.403
0.421IleTrp: 0.421 ± 0.156
1.579IleTyr: 1.579 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
5.316LysAla: 5.316 ± 0.504
0.474LysCys: 0.474 ± 0.178
3.316LysAsp: 3.316 ± 0.339
3.368LysGlu: 3.368 ± 0.49
1.842LysPhe: 1.842 ± 0.348
3.316LysGly: 3.316 ± 0.475
1.368LysHis: 1.368 ± 0.297
2.579LysIle: 2.579 ± 0.433
3.737LysLys: 3.737 ± 0.487
4.052LysLeu: 4.052 ± 0.452
1.947LysMet: 1.947 ± 0.333
2.421LysAsn: 2.421 ± 0.321
2.842LysPro: 2.842 ± 0.378
1.526LysGln: 1.526 ± 0.266
3.21LysArg: 3.21 ± 0.435
3.052LysSer: 3.052 ± 0.413
3.631LysThr: 3.631 ± 0.418
4.526LysVal: 4.526 ± 0.482
1.053LysTrp: 1.053 ± 0.279
2.21LysTyr: 2.21 ± 0.343
0.0LysXaa: 0.0 ± 0.0
Leu
6.684LeuAla: 6.684 ± 0.607
0.789LeuCys: 0.789 ± 0.182
5.105LeuAsp: 5.105 ± 0.569
3.947LeuGlu: 3.947 ± 0.523
2.105LeuPhe: 2.105 ± 0.332
4.737LeuGly: 4.737 ± 0.462
1.053LeuHis: 1.053 ± 0.227
4.894LeuIle: 4.894 ± 0.65
4.579LeuLys: 4.579 ± 0.59
4.421LeuLeu: 4.421 ± 0.461
2.474LeuMet: 2.474 ± 0.353
3.526LeuAsn: 3.526 ± 0.447
4.316LeuPro: 4.316 ± 0.704
3.316LeuGln: 3.316 ± 0.494
4.526LeuArg: 4.526 ± 0.376
4.789LeuSer: 4.789 ± 0.586
5.684LeuThr: 5.684 ± 0.5
4.579LeuVal: 4.579 ± 0.542
1.263LeuTrp: 1.263 ± 0.203
2.421LeuTyr: 2.421 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
3.158MetAla: 3.158 ± 0.456
0.368MetCys: 0.368 ± 0.118
1.579MetAsp: 1.579 ± 0.282
1.684MetGlu: 1.684 ± 0.345
1.263MetPhe: 1.263 ± 0.276
1.789MetGly: 1.789 ± 0.33
0.316MetHis: 0.316 ± 0.104
1.526MetIle: 1.526 ± 0.304
1.526MetLys: 1.526 ± 0.327
2.684MetLeu: 2.684 ± 0.394
1.0MetMet: 1.0 ± 0.252
1.0MetAsn: 1.0 ± 0.242
1.684MetPro: 1.684 ± 0.248
1.158MetGln: 1.158 ± 0.297
2.053MetArg: 2.053 ± 0.273
2.579MetSer: 2.579 ± 0.36
2.631MetThr: 2.631 ± 0.315
2.0MetVal: 2.0 ± 0.343
0.474MetTrp: 0.474 ± 0.146
1.421MetTyr: 1.421 ± 0.264
0.0MetXaa: 0.0 ± 0.0
Asn
3.789AsnAla: 3.789 ± 0.492
0.316AsnCys: 0.316 ± 0.139
2.789AsnAsp: 2.789 ± 0.454
2.105AsnGlu: 2.105 ± 0.338
1.526AsnPhe: 1.526 ± 0.243
4.526AsnGly: 4.526 ± 0.515
1.105AsnHis: 1.105 ± 0.344
2.474AsnIle: 2.474 ± 0.305
2.053AsnLys: 2.053 ± 0.365
3.052AsnLeu: 3.052 ± 0.368
1.316AsnMet: 1.316 ± 0.211
2.0AsnAsn: 2.0 ± 0.328
2.526AsnPro: 2.526 ± 0.413
1.789AsnGln: 1.789 ± 0.309
2.105AsnArg: 2.105 ± 0.321
2.631AsnSer: 2.631 ± 0.398
2.631AsnThr: 2.631 ± 0.386
2.263AsnVal: 2.263 ± 0.369
0.632AsnTrp: 0.632 ± 0.192
1.579AsnTyr: 1.579 ± 0.276
0.0AsnXaa: 0.0 ± 0.0
Pro
3.263ProAla: 3.263 ± 0.542
0.526ProCys: 0.526 ± 0.201
3.631ProAsp: 3.631 ± 0.552
3.21ProGlu: 3.21 ± 0.534
1.368ProPhe: 1.368 ± 0.244
3.263ProGly: 3.263 ± 0.443
0.947ProHis: 0.947 ± 0.199
2.789ProIle: 2.789 ± 0.508
2.368ProLys: 2.368 ± 0.342
3.052ProLeu: 3.052 ± 0.475
1.368ProMet: 1.368 ± 0.26
2.263ProAsn: 2.263 ± 0.377
2.316ProPro: 2.316 ± 0.5
1.368ProGln: 1.368 ± 0.328
1.947ProArg: 1.947 ± 0.344
3.052ProSer: 3.052 ± 0.455
3.21ProThr: 3.21 ± 0.398
3.105ProVal: 3.105 ± 0.422
0.632ProTrp: 0.632 ± 0.179
1.789ProTyr: 1.789 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
4.105GlnAla: 4.105 ± 0.59
0.211GlnCys: 0.211 ± 0.106
2.158GlnAsp: 2.158 ± 0.351
1.526GlnGlu: 1.526 ± 0.281
2.0GlnPhe: 2.0 ± 0.353
2.158GlnGly: 2.158 ± 0.304
0.368GlnHis: 0.368 ± 0.136
2.421GlnIle: 2.421 ± 0.316
1.895GlnLys: 1.895 ± 0.29
3.263GlnLeu: 3.263 ± 0.42
1.474GlnMet: 1.474 ± 0.313
1.368GlnAsn: 1.368 ± 0.269
1.316GlnPro: 1.316 ± 0.319
2.21GlnGln: 2.21 ± 0.518
2.368GlnArg: 2.368 ± 0.369
2.421GlnSer: 2.421 ± 0.39
2.105GlnThr: 2.105 ± 0.379
3.368GlnVal: 3.368 ± 0.632
0.737GlnTrp: 0.737 ± 0.177
1.263GlnTyr: 1.263 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
4.316ArgAla: 4.316 ± 0.613
0.895ArgCys: 0.895 ± 0.207
3.158ArgAsp: 3.158 ± 0.394
2.947ArgGlu: 2.947 ± 0.433
2.895ArgPhe: 2.895 ± 0.36
3.421ArgGly: 3.421 ± 0.43
0.895ArgHis: 0.895 ± 0.249
2.842ArgIle: 2.842 ± 0.267
2.842ArgLys: 2.842 ± 0.389
4.894ArgLeu: 4.894 ± 0.529
2.263ArgMet: 2.263 ± 0.412
2.316ArgAsn: 2.316 ± 0.324
1.947ArgPro: 1.947 ± 0.354
2.421ArgGln: 2.421 ± 0.389
3.368ArgArg: 3.368 ± 0.452
2.737ArgSer: 2.737 ± 0.392
2.789ArgThr: 2.789 ± 0.346
4.0ArgVal: 4.0 ± 0.411
0.789ArgTrp: 0.789 ± 0.194
2.158ArgTyr: 2.158 ± 0.364
0.0ArgXaa: 0.0 ± 0.0
Ser
4.947SerAla: 4.947 ± 0.407
0.526SerCys: 0.526 ± 0.175
3.526SerAsp: 3.526 ± 0.345
2.684SerGlu: 2.684 ± 0.426
2.368SerPhe: 2.368 ± 0.348
4.263SerGly: 4.263 ± 0.502
0.947SerHis: 0.947 ± 0.248
3.263SerIle: 3.263 ± 0.413
3.368SerLys: 3.368 ± 0.471
4.368SerLeu: 4.368 ± 0.427
1.368SerMet: 1.368 ± 0.285
3.158SerAsn: 3.158 ± 0.484
2.737SerPro: 2.737 ± 0.358
3.0SerGln: 3.0 ± 0.511
3.0SerArg: 3.0 ± 0.394
3.052SerSer: 3.052 ± 0.294
3.684SerThr: 3.684 ± 0.531
4.894SerVal: 4.894 ± 0.796
0.947SerTrp: 0.947 ± 0.199
2.0SerTyr: 2.0 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
6.842ThrAla: 6.842 ± 0.646
0.947ThrCys: 0.947 ± 0.239
3.631ThrAsp: 3.631 ± 0.405
2.474ThrGlu: 2.474 ± 0.328
1.579ThrPhe: 1.579 ± 0.242
5.263ThrGly: 5.263 ± 0.548
0.737ThrHis: 0.737 ± 0.197
3.737ThrIle: 3.737 ± 0.511
4.105ThrLys: 4.105 ± 0.545
5.105ThrLeu: 5.105 ± 0.479
1.526ThrMet: 1.526 ± 0.275
2.158ThrAsn: 2.158 ± 0.328
3.158ThrPro: 3.158 ± 0.369
3.158ThrGln: 3.158 ± 0.429
3.158ThrArg: 3.158 ± 0.43
3.947ThrSer: 3.947 ± 0.583
5.368ThrThr: 5.368 ± 0.566
5.526ThrVal: 5.526 ± 0.491
0.947ThrTrp: 0.947 ± 0.201
2.316ThrTyr: 2.316 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
6.052ValAla: 6.052 ± 0.727
0.632ValCys: 0.632 ± 0.212
5.631ValAsp: 5.631 ± 0.488
4.526ValGlu: 4.526 ± 0.488
2.368ValPhe: 2.368 ± 0.382
4.737ValGly: 4.737 ± 0.476
0.789ValHis: 0.789 ± 0.217
4.684ValIle: 4.684 ± 0.5
4.579ValLys: 4.579 ± 0.457
4.947ValLeu: 4.947 ± 0.511
2.579ValMet: 2.579 ± 0.377
3.052ValAsn: 3.052 ± 0.359
3.158ValPro: 3.158 ± 0.516
2.895ValGln: 2.895 ± 0.5
3.789ValArg: 3.789 ± 0.458
4.684ValSer: 4.684 ± 0.602
4.473ValThr: 4.473 ± 0.575
4.947ValVal: 4.947 ± 0.598
1.263ValTrp: 1.263 ± 0.231
2.158ValTyr: 2.158 ± 0.428
0.0ValXaa: 0.0 ± 0.0
Trp
1.737TrpAla: 1.737 ± 0.265
0.211TrpCys: 0.211 ± 0.122
1.474TrpAsp: 1.474 ± 0.317
1.158TrpGlu: 1.158 ± 0.234
0.579TrpPhe: 0.579 ± 0.181
1.0TrpGly: 1.0 ± 0.261
0.316TrpHis: 0.316 ± 0.114
0.737TrpIle: 0.737 ± 0.191
0.526TrpLys: 0.526 ± 0.146
1.789TrpLeu: 1.789 ± 0.311
0.474TrpMet: 0.474 ± 0.157
0.632TrpAsn: 0.632 ± 0.16
0.368TrpPro: 0.368 ± 0.139
0.789TrpGln: 0.789 ± 0.172
0.526TrpArg: 0.526 ± 0.208
1.105TrpSer: 1.105 ± 0.224
1.053TrpThr: 1.053 ± 0.267
1.21TrpVal: 1.21 ± 0.261
0.474TrpTrp: 0.474 ± 0.192
0.632TrpTyr: 0.632 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.0TyrAla: 3.0 ± 0.343
0.474TyrCys: 0.474 ± 0.195
2.526TyrAsp: 2.526 ± 0.313
2.895TyrGlu: 2.895 ± 0.381
1.316TyrPhe: 1.316 ± 0.303
2.421TyrGly: 2.421 ± 0.452
0.789TyrHis: 0.789 ± 0.191
2.053TyrIle: 2.053 ± 0.358
1.684TyrLys: 1.684 ± 0.305
2.526TyrLeu: 2.526 ± 0.374
0.947TyrMet: 0.947 ± 0.213
1.316TyrAsn: 1.316 ± 0.301
2.0TyrPro: 2.0 ± 0.409
1.316TyrGln: 1.316 ± 0.25
1.947TyrArg: 1.947 ± 0.339
2.21TyrSer: 2.21 ± 0.302
2.737TyrThr: 2.737 ± 0.393
2.263TyrVal: 2.263 ± 0.291
0.526TyrTrp: 0.526 ± 0.208
1.368TyrTyr: 1.368 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (19002 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski