Anaerotruncus sp. CAG:528

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaerotruncus; environmental samples

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1705 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5Y696|R5Y696_9FIRM Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1 OS=Anaerotruncus sp. CAG:528 OX=1262700 GN=BN695_00471 PE=4 SV=1
MM1 pKa = 7.87ADD3 pKa = 3.67YY4 pKa = 10.98EE5 pKa = 4.51NNLNNTEE12 pKa = 4.28TTSGGAPQPNTNAQVQPASEE32 pKa = 4.29PKK34 pKa = 9.76PEE36 pKa = 4.43QINEE40 pKa = 4.11PAQGAQSAPSYY51 pKa = 10.95AEE53 pKa = 3.93PQGQSYY59 pKa = 7.22TTSPQEE65 pKa = 3.81DD66 pKa = 4.27TYY68 pKa = 11.58YY69 pKa = 11.17APQANQAPQGNYY81 pKa = 8.33NQPGYY86 pKa = 9.65QAPPQQAYY94 pKa = 8.84YY95 pKa = 10.6APQNGAQVPPYY106 pKa = 10.79GMPQQPVEE114 pKa = 4.31QKK116 pKa = 11.02ASVGLAILSFFIPIAGLIIFLTKK139 pKa = 10.63KK140 pKa = 10.15NDD142 pKa = 3.49RR143 pKa = 11.84PKK145 pKa = 8.33TAKK148 pKa = 10.37VSGICALVSFILNIVIGVIGGVIGGVAATKK178 pKa = 10.1IAEE181 pKa = 4.56NPDD184 pKa = 3.29QLDD187 pKa = 3.64SYY189 pKa = 11.11ISDD192 pKa = 4.82AIGDD196 pKa = 3.81NSDD199 pKa = 3.55NLVDD203 pKa = 4.78GDD205 pKa = 3.84EE206 pKa = 4.92LGNFVCDD213 pKa = 3.51VTDD216 pKa = 3.48VSKK219 pKa = 8.23TTDD222 pKa = 3.32ANGKK226 pKa = 7.82PAIIVTYY233 pKa = 8.62YY234 pKa = 10.49FKK236 pKa = 11.29NNSDD240 pKa = 3.43TAIDD244 pKa = 3.99FDD246 pKa = 4.09SALYY250 pKa = 9.73TIVKK254 pKa = 10.35QNDD257 pKa = 2.99NTLIRR262 pKa = 11.84TVLGTSDD269 pKa = 3.96DD270 pKa = 3.71TDD272 pKa = 4.07LADD275 pKa = 3.82TTATEE280 pKa = 4.35PGATSKK286 pKa = 10.79VKK288 pKa = 10.23RR289 pKa = 11.84AYY291 pKa = 9.08TLNDD295 pKa = 3.05QTSDD299 pKa = 3.78VVFEE303 pKa = 5.29LYY305 pKa = 10.93DD306 pKa = 3.6NTDD309 pKa = 3.75EE310 pKa = 4.41NNMYY314 pKa = 10.05YY315 pKa = 10.62LEE317 pKa = 4.2YY318 pKa = 10.66TFTLGNN324 pKa = 3.53

Molecular weight: 34.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5XWP9|R5XWP9_9FIRM Predicted spore germination protein OS=Anaerotruncus sp. CAG:528 OX=1262700 GN=BN695_01562 PE=3 SV=1
MM1 pKa = 7.78IKK3 pKa = 9.22GTNRR7 pKa = 11.84KK8 pKa = 9.06CVFTPGYY15 pKa = 8.97KK16 pKa = 9.6PRR18 pKa = 11.84NIAIGVPRR26 pKa = 11.84NRR28 pKa = 11.84GRR30 pKa = 11.84GLPRR34 pKa = 11.84TVLRR38 pKa = 11.84FIPNLISVCPAYY50 pKa = 10.07GVPINMRR57 pKa = 11.84RR58 pKa = 11.84AAGLGINGKK67 pKa = 8.01ILRR70 pKa = 11.84CRR72 pKa = 11.84QRR74 pKa = 11.84IAVFNLHH81 pKa = 6.48ARR83 pKa = 11.84VFMPTAAHH91 pKa = 5.97CFVYY95 pKa = 10.68SVFNRR100 pKa = 11.84IRR102 pKa = 11.84IEE104 pKa = 3.68RR105 pKa = 11.84GRR107 pKa = 11.84ASKK110 pKa = 10.25FNKK113 pKa = 9.89RR114 pKa = 11.84GFCAQICLCPTEE126 pKa = 4.85NISLIYY132 pKa = 10.4SVMVALISGKK142 pKa = 9.97IICASVNAYY151 pKa = 10.14QIGITEE157 pKa = 4.56HH158 pKa = 6.61GIKK161 pKa = 8.9PTVKK165 pKa = 9.66INVNIHH171 pKa = 6.55FIFIHH176 pKa = 6.88KK177 pKa = 9.31IAQGAAFARR186 pKa = 11.84LEE188 pKa = 4.01FARR191 pKa = 11.84YY192 pKa = 7.44EE193 pKa = 4.28IPVHH197 pKa = 5.49IHH199 pKa = 5.96IIRR202 pKa = 11.84RR203 pKa = 11.84ASAEE207 pKa = 4.1CTGFCAVVVFAVNSAIRR224 pKa = 11.84IGAGHH229 pKa = 6.51YY230 pKa = 9.41NQTIIPKK237 pKa = 9.48IIRR240 pKa = 11.84IFGEE244 pKa = 3.59IFHH247 pKa = 6.65NVICGINKK255 pKa = 8.77QFTVSVITIFKK266 pKa = 8.18TVNRR270 pKa = 11.84KK271 pKa = 7.11GTKK274 pKa = 8.95QSFAVVAAAVYY285 pKa = 10.26NKK287 pKa = 9.82RR288 pKa = 11.84LQIAAEE294 pKa = 4.01RR295 pKa = 11.84TFAINDD301 pKa = 3.38FCYY304 pKa = 10.32II305 pKa = 3.78

Molecular weight: 34.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1705

0

1705

549919

32

2274

322.5

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.707 ± 0.062

1.817 ± 0.025

5.798 ± 0.055

6.699 ± 0.065

4.431 ± 0.043

7.176 ± 0.049

1.481 ± 0.025

7.429 ± 0.059

7.592 ± 0.04

8.21 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.026

5.082 ± 0.052

3.317 ± 0.033

2.597 ± 0.029

3.697 ± 0.046

6.455 ± 0.059

5.217 ± 0.061

6.896 ± 0.049

0.769 ± 0.021

4.022 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski