Amino acid dipepetide frequency for Flavobacterium sp. Leaf359

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.544AlaAla: 4.544 ± 0.1	0.544AlaCys: 0.544 ± 0.023	3.368AlaAsp: 3.368 ± 0.074	4.194AlaGlu: 4.194 ± 0.074	3.425AlaPhe: 3.425 ± 0.063	4.472AlaGly: 4.472 ± 0.09	1.004AlaHis: 1.004 ± 0.028	5.527AlaIle: 5.527 ± 0.078	5.026AlaLys: 5.026 ± 0.099	6.348AlaLeu: 6.348 ± 0.088	1.532AlaMet: 1.532 ± 0.045	3.532AlaAsn: 3.532 ± 0.08	2.025AlaPro: 2.025 ± 0.053	2.496AlaGln: 2.496 ± 0.048	2.04AlaArg: 2.04 ± 0.052	4.398AlaSer: 4.398 ± 0.077	4.224AlaThr: 4.224 ± 0.118	4.454AlaVal: 4.454 ± 0.073	0.6AlaTrp: 0.6 ± 0.024	2.558AlaTyr: 2.558 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.461CysAla: 0.461 ± 0.03	0.101CysCys: 0.101 ± 0.01	0.415CysAsp: 0.415 ± 0.022	0.451CysGlu: 0.451 ± 0.026	0.386CysPhe: 0.386 ± 0.018	0.65CysGly: 0.65 ± 0.033	0.192CysHis: 0.192 ± 0.014	0.56CysIle: 0.56 ± 0.024	0.43CysLys: 0.43 ± 0.022	0.658CysLeu: 0.658 ± 0.026	0.121CysMet: 0.121 ± 0.01	0.429CysAsn: 0.429 ± 0.023	0.311CysPro: 0.311 ± 0.02	0.266CysGln: 0.266 ± 0.021	0.223CysArg: 0.223 ± 0.015	0.58CysSer: 0.58 ± 0.028	0.446CysThr: 0.446 ± 0.028	0.41CysVal: 0.41 ± 0.02	0.061CysTrp: 0.061 ± 0.008	0.295CysTyr: 0.295 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.536AspAla: 3.536 ± 0.062	0.42AspCys: 0.42 ± 0.021	2.671AspAsp: 2.671 ± 0.059	3.563AspGlu: 3.563 ± 0.063	3.699AspPhe: 3.699 ± 0.064	3.589AspGly: 3.589 ± 0.071	0.744AspHis: 0.744 ± 0.026	4.248AspIle: 4.248 ± 0.064	4.228AspLys: 4.228 ± 0.08	4.761AspLeu: 4.761 ± 0.07	1.097AspMet: 1.097 ± 0.033	3.179AspAsn: 3.179 ± 0.06	1.559AspPro: 1.559 ± 0.046	1.268AspGln: 1.268 ± 0.036	1.884AspArg: 1.884 ± 0.042	3.162AspSer: 3.162 ± 0.065	2.675AspThr: 2.675 ± 0.055	3.284AspVal: 3.284 ± 0.054	0.69AspTrp: 0.69 ± 0.025	2.82AspTyr: 2.82 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	4.487GluAla: 4.487 ± 0.077	0.323GluCys: 0.323 ± 0.02	3.138GluAsp: 3.138 ± 0.052	4.888GluGlu: 4.888 ± 0.093	2.942GluPhe: 2.942 ± 0.061	3.469GluGly: 3.469 ± 0.062	1.023GluHis: 1.023 ± 0.029	5.795GluIle: 5.795 ± 0.085	6.773GluLys: 6.773 ± 0.102	5.84GluLeu: 5.84 ± 0.088	1.681GluMet: 1.681 ± 0.036	4.949GluAsn: 4.949 ± 0.073	1.554GluPro: 1.554 ± 0.043	2.24GluGln: 2.24 ± 0.055	2.484GluArg: 2.484 ± 0.054	3.359GluSer: 3.359 ± 0.06	3.603GluThr: 3.603 ± 0.062	3.97GluVal: 3.97 ± 0.072	0.574GluTrp: 0.574 ± 0.025	2.486GluTyr: 2.486 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	3.326PheAla: 3.326 ± 0.065	0.497PheCys: 0.497 ± 0.028	3.235PheAsp: 3.235 ± 0.06	3.432PheGlu: 3.432 ± 0.064	2.982PhePhe: 2.982 ± 0.056	3.692PheGly: 3.692 ± 0.064	0.836PheHis: 0.836 ± 0.029	3.88PheIle: 3.88 ± 0.082	3.479PheLys: 3.479 ± 0.064	4.961PheLeu: 4.961 ± 0.095	1.114PheMet: 1.114 ± 0.033	3.114PheAsn: 3.114 ± 0.055	1.798PhePro: 1.798 ± 0.045	1.68PheGln: 1.68 ± 0.045	1.798PheArg: 1.798 ± 0.039	4.232PheSer: 4.232 ± 0.074	3.241PheThr: 3.241 ± 0.068	3.049PheVal: 3.049 ± 0.054	0.558PheTrp: 0.558 ± 0.02	2.291PheTyr: 2.291 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.078GlyAla: 4.078 ± 0.071	0.611GlyCys: 0.611 ± 0.03	3.1GlyAsp: 3.1 ± 0.051	3.409GlyGlu: 3.409 ± 0.057	3.632GlyPhe: 3.632 ± 0.064	4.38GlyGly: 4.38 ± 0.107	1.111GlyHis: 1.111 ± 0.033	5.583GlyIle: 5.583 ± 0.087	5.332GlyLys: 5.332 ± 0.084	5.588GlyLeu: 5.588 ± 0.073	1.558GlyMet: 1.558 ± 0.039	4.068GlyAsn: 4.068 ± 0.076	1.32GlyPro: 1.32 ± 0.042	2.063GlyGln: 2.063 ± 0.053	2.076GlyArg: 2.076 ± 0.046	4.065GlySer: 4.065 ± 0.079	4.513GlyThr: 4.513 ± 0.132	3.849GlyVal: 3.849 ± 0.068	0.698GlyTrp: 0.698 ± 0.031	2.918GlyTyr: 2.918 ± 0.05	0.001GlyXaa: 0.001 ± 0.001
His	0.892HisAla: 0.892 ± 0.028	0.16HisCys: 0.16 ± 0.013	0.837HisAsp: 0.837 ± 0.031	1.002HisGlu: 1.002 ± 0.035	1.145HisPhe: 1.145 ± 0.04	0.99HisGly: 0.99 ± 0.03	0.411HisHis: 0.411 ± 0.021	1.326HisIle: 1.326 ± 0.035	1.158HisLys: 1.158 ± 0.033	1.609HisLeu: 1.609 ± 0.036	0.298HisMet: 0.298 ± 0.016	0.956HisAsn: 0.956 ± 0.027	0.771HisPro: 0.771 ± 0.031	0.648HisGln: 0.648 ± 0.027	0.618HisArg: 0.618 ± 0.026	1.022HisSer: 1.022 ± 0.03	0.928HisThr: 0.928 ± 0.029	0.797HisVal: 0.797 ± 0.031	0.163HisTrp: 0.163 ± 0.011	0.804HisTyr: 0.804 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.861IleAla: 5.861 ± 0.092	0.636IleCys: 0.636 ± 0.03	4.566IleAsp: 4.566 ± 0.068	5.317IleGlu: 5.317 ± 0.081	3.589IlePhe: 3.589 ± 0.066	4.996IleGly: 4.996 ± 0.077	1.316IleHis: 1.316 ± 0.036	6.026IleIle: 6.026 ± 0.098	5.732IleLys: 5.732 ± 0.09	6.924IleLeu: 6.924 ± 0.098	1.395IleMet: 1.395 ± 0.038	4.573IleAsn: 4.573 ± 0.069	3.2IlePro: 3.2 ± 0.061	2.838IleGln: 2.838 ± 0.056	2.821IleArg: 2.821 ± 0.052	5.685IleSer: 5.685 ± 0.073	4.901IleThr: 4.901 ± 0.101	4.948IleVal: 4.948 ± 0.075	0.716IleTrp: 0.716 ± 0.025	2.919IleTyr: 2.919 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	5.368LysAla: 5.368 ± 0.1	0.329LysCys: 0.329 ± 0.019	4.379LysAsp: 4.379 ± 0.079	6.316LysGlu: 6.316 ± 0.102	3.1LysPhe: 3.1 ± 0.059	4.462LysGly: 4.462 ± 0.074	1.218LysHis: 1.218 ± 0.033	6.918LysIle: 6.918 ± 0.103	7.458LysLys: 7.458 ± 0.108	6.603LysLeu: 6.603 ± 0.101	2.093LysMet: 2.093 ± 0.049	5.704LysAsn: 5.704 ± 0.086	2.546LysPro: 2.546 ± 0.054	2.705LysGln: 2.705 ± 0.053	2.598LysArg: 2.598 ± 0.059	4.572LysSer: 4.572 ± 0.074	4.92LysThr: 4.92 ± 0.071	4.463LysVal: 4.463 ± 0.066	0.762LysTrp: 0.762 ± 0.029	3.013LysTyr: 3.013 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	5.987LeuAla: 5.987 ± 0.084	0.648LeuCys: 0.648 ± 0.023	4.828LeuAsp: 4.828 ± 0.071	5.984LeuGlu: 5.984 ± 0.084	5.143LeuPhe: 5.143 ± 0.087	5.577LeuGly: 5.577 ± 0.087	1.563LeuHis: 1.563 ± 0.04	6.351LeuIle: 6.351 ± 0.104	7.631LeuLys: 7.631 ± 0.111	9.193LeuLeu: 9.193 ± 0.134	2.094LeuMet: 2.094 ± 0.052	5.302LeuAsn: 5.302 ± 0.083	3.574LeuPro: 3.574 ± 0.058	3.299LeuGln: 3.299 ± 0.064	3.048LeuArg: 3.048 ± 0.063	6.696LeuSer: 6.696 ± 0.087	5.109LeuThr: 5.109 ± 0.087	5.128LeuVal: 5.128 ± 0.064	0.772LeuTrp: 0.772 ± 0.027	3.309LeuTyr: 3.309 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	1.683MetAla: 1.683 ± 0.046	0.109MetCys: 0.109 ± 0.009	1.136MetAsp: 1.136 ± 0.034	1.501MetGlu: 1.501 ± 0.04	0.842MetPhe: 0.842 ± 0.028	1.352MetGly: 1.352 ± 0.038	0.385MetHis: 0.385 ± 0.018	1.577MetIle: 1.577 ± 0.042	2.368MetLys: 2.368 ± 0.048	1.989MetLeu: 1.989 ± 0.046	0.619MetMet: 0.619 ± 0.027	1.37MetAsn: 1.37 ± 0.039	0.869MetPro: 0.869 ± 0.033	0.895MetGln: 0.895 ± 0.027	0.819MetArg: 0.819 ± 0.028	1.383MetSer: 1.383 ± 0.036	1.221MetThr: 1.221 ± 0.036	1.269MetVal: 1.269 ± 0.034	0.151MetTrp: 0.151 ± 0.013	0.664MetTyr: 0.664 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	4.071AsnAla: 4.071 ± 0.065	0.489AsnCys: 0.489 ± 0.037	3.2AsnAsp: 3.2 ± 0.057	3.669AsnGlu: 3.669 ± 0.056	3.227AsnPhe: 3.227 ± 0.061	4.423AsnGly: 4.423 ± 0.104	1.067AsnHis: 1.067 ± 0.03	4.638AsnIle: 4.638 ± 0.068	4.162AsnLys: 4.162 ± 0.071	5.402AsnLeu: 5.402 ± 0.08	1.342AsnMet: 1.342 ± 0.035	3.867AsnAsn: 3.867 ± 0.081	3.133AsnPro: 3.133 ± 0.063	2.186AsnGln: 2.186 ± 0.053	2.223AsnArg: 2.223 ± 0.049	4.001AsnSer: 4.001 ± 0.078	3.688AsnThr: 3.688 ± 0.083	3.567AsnVal: 3.567 ± 0.069	0.741AsnTrp: 0.741 ± 0.03	2.914AsnTyr: 2.914 ± 0.061	0.0AsnXaa: 0.0 ± 0.0
Pro	2.358ProAla: 2.358 ± 0.064	0.202ProCys: 0.202 ± 0.014	2.147ProAsp: 2.147 ± 0.044	2.883ProGlu: 2.883 ± 0.058	1.926ProPhe: 1.926 ± 0.047	2.075ProGly: 2.075 ± 0.059	0.52ProHis: 0.52 ± 0.023	2.562ProIle: 2.562 ± 0.047	2.682ProLys: 2.682 ± 0.064	2.882ProLeu: 2.882 ± 0.057	0.728ProMet: 0.728 ± 0.025	2.243ProAsn: 2.243 ± 0.065	0.845ProPro: 0.845 ± 0.03	1.178ProGln: 1.178 ± 0.036	0.842ProArg: 0.842 ± 0.029	2.085ProSer: 2.085 ± 0.052	2.057ProThr: 2.057 ± 0.074	2.582ProVal: 2.582 ± 0.046	0.286ProTrp: 0.286 ± 0.016	1.449ProTyr: 1.449 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.121GlnAla: 2.121 ± 0.046	0.192GlnCys: 0.192 ± 0.019	1.624GlnAsp: 1.624 ± 0.037	2.358GlnGlu: 2.358 ± 0.046	1.744GlnPhe: 1.744 ± 0.043	1.801GlnGly: 1.801 ± 0.043	0.565GlnHis: 0.565 ± 0.026	2.617GlnIle: 2.617 ± 0.054	3.26GlnLys: 3.26 ± 0.066	3.364GlnLeu: 3.364 ± 0.053	0.849GlnMet: 0.849 ± 0.027	2.484GlnAsn: 2.484 ± 0.049	1.135GlnPro: 1.135 ± 0.037	1.487GlnGln: 1.487 ± 0.041	1.155GlnArg: 1.155 ± 0.035	2.003GlnSer: 2.003 ± 0.041	1.987GlnThr: 1.987 ± 0.051	1.958GlnVal: 1.958 ± 0.044	0.368GlnTrp: 0.368 ± 0.02	1.428GlnTyr: 1.428 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	1.941ArgAla: 1.941 ± 0.046	0.209ArgCys: 0.209 ± 0.014	1.647ArgAsp: 1.647 ± 0.041	2.274ArgGlu: 2.274 ± 0.051	1.909ArgPhe: 1.909 ± 0.041	1.732ArgGly: 1.732 ± 0.042	0.583ArgHis: 0.583 ± 0.025	3.009ArgIle: 3.009 ± 0.052	2.929ArgLys: 2.929 ± 0.062	3.323ArgLeu: 3.323 ± 0.068	0.939ArgMet: 0.939 ± 0.032	2.306ArgAsn: 2.306 ± 0.053	1.092ArgPro: 1.092 ± 0.034	1.193ArgGln: 1.193 ± 0.036	1.281ArgArg: 1.281 ± 0.036	1.843ArgSer: 1.843 ± 0.045	1.829ArgThr: 1.829 ± 0.039	2.002ArgVal: 2.002 ± 0.043	0.396ArgTrp: 0.396 ± 0.018	1.506ArgTyr: 1.506 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.819SerAla: 3.819 ± 0.069	0.697SerCys: 0.697 ± 0.032	3.447SerAsp: 3.447 ± 0.053	4.034SerGlu: 4.034 ± 0.065	3.807SerPhe: 3.807 ± 0.065	5.018SerGly: 5.018 ± 0.101	1.104SerHis: 1.104 ± 0.037	4.91SerIle: 4.91 ± 0.071	4.83SerLys: 4.83 ± 0.078	6.097SerLeu: 6.097 ± 0.086	1.351SerMet: 1.351 ± 0.037	3.806SerAsn: 3.806 ± 0.081	2.22SerPro: 2.22 ± 0.049	2.378SerGln: 2.378 ± 0.053	2.232SerArg: 2.232 ± 0.046	4.287SerSer: 4.287 ± 0.077	3.421SerThr: 3.421 ± 0.082	4.102SerVal: 4.102 ± 0.068	0.667SerTrp: 0.667 ± 0.028	2.881SerTyr: 2.881 ± 0.061	0.001SerXaa: 0.001 ± 0.001
Thr	4.458ThrAla: 4.458 ± 0.132	0.354ThrCys: 0.354 ± 0.022	3.197ThrAsp: 3.197 ± 0.067	3.443ThrGlu: 3.443 ± 0.05	3.113ThrPhe: 3.113 ± 0.051	4.451ThrGly: 4.451 ± 0.108	0.942ThrHis: 0.942 ± 0.034	5.065ThrIle: 5.065 ± 0.09	3.828ThrLys: 3.828 ± 0.056	5.281ThrLeu: 5.281 ± 0.087	0.936ThrMet: 0.936 ± 0.03	3.26ThrAsn: 3.26 ± 0.075	2.629ThrPro: 2.629 ± 0.077	1.98ThrGln: 1.98 ± 0.05	1.687ThrArg: 1.687 ± 0.044	3.805ThrSer: 3.805 ± 0.081	3.843ThrThr: 3.843 ± 0.115	4.319ThrVal: 4.319 ± 0.133	0.556ThrTrp: 0.556 ± 0.028	2.397ThrTyr: 2.397 ± 0.066	0.001ThrXaa: 0.001 ± 0.001
Val	4.215ValAla: 4.215 ± 0.077	0.512ValCys: 0.512 ± 0.023	3.143ValAsp: 3.143 ± 0.051	3.73ValGlu: 3.73 ± 0.068	3.427ValPhe: 3.427 ± 0.064	3.637ValGly: 3.637 ± 0.063	0.948ValHis: 0.948 ± 0.031	4.721ValIle: 4.721 ± 0.077	4.415ValLys: 4.415 ± 0.07	5.745ValLeu: 5.745 ± 0.072	1.365ValMet: 1.365 ± 0.04	3.552ValAsn: 3.552 ± 0.062	2.256ValPro: 2.256 ± 0.042	1.801ValGln: 1.801 ± 0.044	2.119ValArg: 2.119 ± 0.046	4.48ValSer: 4.48 ± 0.069	3.903ValThr: 3.903 ± 0.112	3.945ValVal: 3.945 ± 0.07	0.594ValTrp: 0.594 ± 0.024	2.43ValTyr: 2.43 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.622TrpAla: 0.622 ± 0.027	0.085TrpCys: 0.085 ± 0.009	0.546TrpAsp: 0.546 ± 0.024	0.633TrpGlu: 0.633 ± 0.026	0.529TrpPhe: 0.529 ± 0.023	0.585TrpGly: 0.585 ± 0.025	0.212TrpHis: 0.212 ± 0.015	0.775TrpIle: 0.775 ± 0.027	0.824TrpLys: 0.824 ± 0.03	0.913TrpLeu: 0.913 ± 0.031	0.281TrpMet: 0.281 ± 0.019	0.748TrpAsn: 0.748 ± 0.031	0.187TrpPro: 0.187 ± 0.014	0.399TrpGln: 0.399 ± 0.022	0.339TrpArg: 0.339 ± 0.017	0.59TrpSer: 0.59 ± 0.024	0.569TrpThr: 0.569 ± 0.028	0.523TrpVal: 0.523 ± 0.023	0.129TrpTrp: 0.129 ± 0.011	0.417TrpTyr: 0.417 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.486TyrAla: 2.486 ± 0.045	0.33TyrCys: 0.33 ± 0.019	2.451TyrAsp: 2.451 ± 0.056	2.499TyrGlu: 2.499 ± 0.051	2.63TyrPhe: 2.63 ± 0.055	2.597TyrGly: 2.597 ± 0.053	0.791TyrHis: 0.791 ± 0.031	2.869TyrIle: 2.869 ± 0.062	2.981TyrLys: 2.981 ± 0.055	3.755TyrLeu: 3.755 ± 0.071	0.806TyrMet: 0.806 ± 0.028	2.572TyrAsn: 2.572 ± 0.059	1.508TyrPro: 1.508 ± 0.037	1.5TyrGln: 1.5 ± 0.04	1.655TyrArg: 1.655 ± 0.042	2.861TyrSer: 2.861 ± 0.063	2.464TyrThr: 2.464 ± 0.067	2.319TyrVal: 2.319 ± 0.048	0.423TyrTrp: 0.423 ± 0.02	1.994TyrTyr: 1.994 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.003

Statistics based on 3309 proteins (1096483 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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