Paenibacillus oryzae
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5020 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1A5YC33|A0A1A5YC33_9BACL Hydrolase OS=Paenibacillus oryzae OX=1844972 GN=A7K91_23890 PE=4 SV=1
MM1 pKa = 7.85 AKK3 pKa = 8.96 FTWVEE8 pKa = 3.45 KK9 pKa = 9.45 DD10 pKa = 3.02 TCIACGACGATAPDD24 pKa = 3.47 IYY26 pKa = 11.02 DD27 pKa = 3.64 YY28 pKa = 11.51 DD29 pKa = 4.43 DD30 pKa = 3.97 EE31 pKa = 5.79 GLAEE35 pKa = 4.52 VIFDD39 pKa = 3.5 GDD41 pKa = 3.42 KK42 pKa = 11.07 NRR44 pKa = 11.84 GVTAIPDD51 pKa = 3.59 DD52 pKa = 4.48 MFDD55 pKa = 5.12 DD56 pKa = 4.2 MQDD59 pKa = 3.28 ACDD62 pKa = 3.94 GCPTDD67 pKa = 4.24 SIKK70 pKa = 10.77 IADD73 pKa = 4.02 EE74 pKa = 4.13 PFNAEE79 pKa = 3.85 GG80 pKa = 3.55
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.528
IPC_protein 3.516
Toseland 3.3
ProMoST 3.732
Dawson 3.541
Bjellqvist 3.706
Wikipedia 3.528
Rodwell 3.35
Grimsley 3.21
Solomon 3.516
Lehninger 3.478
Nozaki 3.668
DTASelect 3.935
Thurlkill 3.376
EMBOSS 3.528
Sillero 3.656
Patrickios 0.769
IPC_peptide 3.503
IPC2_peptide 3.617
IPC2.peptide.svr19 3.664
Protein with the highest isoelectric point:
>tr|A0A1A5YM81|A0A1A5YM81_9BACL Uncharacterized protein OS=Paenibacillus oryzae OX=1844972 GN=A7K91_00110 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.84 PNVSKK11 pKa = 10.73 RR12 pKa = 11.84 AKK14 pKa = 8.72 VHH16 pKa = 5.43 GFRR19 pKa = 11.84 KK20 pKa = 10.02 RR21 pKa = 11.84 MSSKK25 pKa = 10.47 NGRR28 pKa = 11.84 KK29 pKa = 9.01 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.23 GRR39 pKa = 11.84 KK40 pKa = 8.56 VLSAA44 pKa = 4.05
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5020
0
5020
1677518
26
3049
334.2
37.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.773 ± 0.042
0.757 ± 0.011
5.06 ± 0.028
6.906 ± 0.034
4.063 ± 0.026
7.824 ± 0.036
2.018 ± 0.018
6.485 ± 0.029
5.222 ± 0.027
10.405 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.811 ± 0.018
3.762 ± 0.028
3.966 ± 0.02
3.757 ± 0.02
5.047 ± 0.027
6.562 ± 0.027
5.05 ± 0.029
6.765 ± 0.03
1.276 ± 0.012
3.489 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here