Giant house spider associated circular virus 1
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BP83|A0A346BP83_9VIRU Putative capsid protein OS=Giant house spider associated circular virus 1 OX=2293288 PE=4 SV=1
MM1 pKa = 7.71 SFRR4 pKa = 11.84 FNHH7 pKa = 6.52 KK8 pKa = 10.25 YY9 pKa = 10.79 VLLTYY14 pKa = 8.91 AQCGDD19 pKa = 3.65 LDD21 pKa = 3.91 GFAVMEE27 pKa = 4.95 KK28 pKa = 9.92 ISSLGAEE35 pKa = 4.25 CIVARR40 pKa = 11.84 EE41 pKa = 4.01 HH42 pKa = 7.03 HH43 pKa = 6.86 EE44 pKa = 4.46 DD45 pKa = 3.68 GGVHH49 pKa = 6.12 LHH51 pKa = 5.86 VFCEE55 pKa = 4.49 FEE57 pKa = 3.92 RR58 pKa = 11.84 KK59 pKa = 8.99 FRR61 pKa = 11.84 SRR63 pKa = 11.84 RR64 pKa = 11.84 ADD66 pKa = 3.25 VFDD69 pKa = 3.64 VDD71 pKa = 4.58 GVHH74 pKa = 7.22 PNIEE78 pKa = 4.13 PSKK81 pKa = 8.32 GTPEE85 pKa = 3.79 KK86 pKa = 10.85 GYY88 pKa = 10.88 DD89 pKa = 3.47 YY90 pKa = 10.69 AIKK93 pKa = 10.71 DD94 pKa = 3.36 GDD96 pKa = 4.31 VICGGLARR104 pKa = 11.84 PSARR108 pKa = 11.84 PTTNGNTFDD117 pKa = 3.79 KK118 pKa = 10.32 WSQITSAEE126 pKa = 4.04 SRR128 pKa = 11.84 DD129 pKa = 3.9 EE130 pKa = 4.2 FWSLVHH136 pKa = 6.73 EE137 pKa = 5.23 LDD139 pKa = 4.49 PKK141 pKa = 10.79 SAACNHH147 pKa = 5.93 SSLVKK152 pKa = 10.17 YY153 pKa = 10.47 CDD155 pKa = 2.5 WKK157 pKa = 10.05 YY158 pKa = 10.79 RR159 pKa = 11.84 EE160 pKa = 5.0 RR161 pKa = 11.84 IPDD164 pKa = 3.51 YY165 pKa = 8.84 KK166 pKa = 10.28 TPAGISFDD174 pKa = 3.43 SGGIDD179 pKa = 5.4 GRR181 pKa = 11.84 DD182 pKa = 2.96 SWLQQSGIGLDD193 pKa = 3.53 EE194 pKa = 4.49 PLVGKK199 pKa = 10.56 LSYY202 pKa = 9.74 TSQGGIPPTGKK213 pKa = 8.15 TLWARR218 pKa = 11.84 SLGAHH223 pKa = 7.15 IYY225 pKa = 9.06 CVGMISGDD233 pKa = 3.51 EE234 pKa = 4.27 CIKK237 pKa = 10.31 TDD239 pKa = 2.81 VDD241 pKa = 3.7 YY242 pKa = 11.49 AIFDD246 pKa = 5.02 DD247 pKa = 3.61 IRR249 pKa = 11.84 GGIKK253 pKa = 9.99 FFPSFKK259 pKa = 10.22 EE260 pKa = 3.81 WLGCQQWITVKK271 pKa = 10.48 RR272 pKa = 11.84 LYY274 pKa = 10.44 RR275 pKa = 11.84 EE276 pKa = 3.79 PKK278 pKa = 9.24 LRR280 pKa = 11.84 LWGKK284 pKa = 8.42 PSIWISNTDD293 pKa = 3.32 PRR295 pKa = 11.84 NEE297 pKa = 3.86 MLQSDD302 pKa = 4.98 VEE304 pKa = 4.35 WMNLNCTFIEE314 pKa = 4.22 VNSAIFHH321 pKa = 6.9 ANIQQ325 pKa = 3.5
Molecular weight: 36.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.557
IPC2_protein 5.55
IPC_protein 5.575
Toseland 5.893
ProMoST 5.881
Dawson 5.804
Bjellqvist 5.83
Wikipedia 5.792
Rodwell 5.779
Grimsley 5.995
Solomon 5.804
Lehninger 5.792
Nozaki 6.046
DTASelect 6.237
Thurlkill 6.211
EMBOSS 6.186
Sillero 6.148
Patrickios 3.986
IPC_peptide 5.817
IPC2_peptide 6.148
IPC2.peptide.svr19 6.114
Protein with the highest isoelectric point:
>tr|A0A346BP83|A0A346BP83_9VIRU Putative capsid protein OS=Giant house spider associated circular virus 1 OX=2293288 PE=4 SV=1
MM1 pKa = 7.53 GKK3 pKa = 8.41 YY4 pKa = 6.03 TTSKK8 pKa = 10.28 SSGRR12 pKa = 11.84 YY13 pKa = 4.63 GTKK16 pKa = 9.59 RR17 pKa = 11.84 YY18 pKa = 9.89 ARR20 pKa = 11.84 RR21 pKa = 11.84 SRR23 pKa = 11.84 TTIKK27 pKa = 9.93 RR28 pKa = 11.84 RR29 pKa = 11.84 YY30 pKa = 6.53 PARR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 9.17 RR36 pKa = 11.84 AYY38 pKa = 8.37 TKK40 pKa = 10.21 RR41 pKa = 11.84 PRR43 pKa = 11.84 PMSRR47 pKa = 11.84 KK48 pKa = 9.59 RR49 pKa = 11.84 ILNVSTTKK57 pKa = 10.52 KK58 pKa = 10.2 RR59 pKa = 11.84 DD60 pKa = 3.25 TLMSYY65 pKa = 10.89 DD66 pKa = 3.44 VARR69 pKa = 11.84 SQNGPAIVSSGSVEE83 pKa = 3.51 RR84 pKa = 11.84 WTTNLLNYY92 pKa = 10.17 LPTSFSSADD101 pKa = 3.22 DD102 pKa = 3.78 DD103 pKa = 4.61 YY104 pKa = 11.89 RR105 pKa = 11.84 RR106 pKa = 11.84 TKK108 pKa = 10.32 NNVYY112 pKa = 10.14 FRR114 pKa = 11.84 GAKK117 pKa = 9.58 LAWNFDD123 pKa = 3.96 MSDD126 pKa = 3.24 SSPWEE131 pKa = 3.41 IRR133 pKa = 11.84 MIAFWGSDD141 pKa = 3.31 GTITANPQSRR151 pKa = 11.84 FYY153 pKa = 9.1 TTITPTGGSPYY164 pKa = 10.0 QVNGLLPVLPGSVPDD179 pKa = 3.87 LRR181 pKa = 11.84 MTLFQGTEE189 pKa = 3.85 GTDD192 pKa = 2.67 WFDD195 pKa = 5.04 AMIAPLDD202 pKa = 3.51 VRR204 pKa = 11.84 RR205 pKa = 11.84 NKK207 pKa = 10.25 IIIDD211 pKa = 3.17 KK212 pKa = 10.87 RR213 pKa = 11.84 MTLRR217 pKa = 11.84 SGNDD221 pKa = 2.9 VGSFKK226 pKa = 10.86 RR227 pKa = 11.84 MNLWVPANKK236 pKa = 9.09 MVRR239 pKa = 11.84 FNNDD243 pKa = 2.6 EE244 pKa = 4.25 NGSDD248 pKa = 3.52 VDD250 pKa = 3.89 KK251 pKa = 11.11 TSGIPSGLYY260 pKa = 10.62 SPDD263 pKa = 3.01 CLYY266 pKa = 11.23 YY267 pKa = 9.44 VTLYY271 pKa = 9.62 KK272 pKa = 10.37 QRR274 pKa = 11.84 GAADD278 pKa = 3.32 TAAQLLVSCNATVYY292 pKa = 8.05 WHH294 pKa = 6.33 EE295 pKa = 4.12 RR296 pKa = 3.32
Molecular weight: 33.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.75
IPC_protein 10.35
Toseland 10.306
ProMoST 10.116
Dawson 10.511
Bjellqvist 10.218
Wikipedia 10.716
Rodwell 10.774
Grimsley 10.584
Solomon 10.555
Lehninger 10.511
Nozaki 10.292
DTASelect 10.218
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.423
Patrickios 10.35
IPC_peptide 10.54
IPC2_peptide 9.077
IPC2.peptide.svr19 8.649
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
621
296
325
310.5
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.636 ± 0.269
1.932 ± 0.759
7.085 ± 0.402
3.865 ± 1.314
4.026 ± 0.595
7.89 ± 0.685
1.771 ± 0.866
5.636 ± 0.955
5.797 ± 0.033
6.441 ± 0.013
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.415 ± 0.582
4.509 ± 0.746
4.992 ± 0.25
2.415 ± 0.235
7.89 ± 1.56
8.696 ± 0.461
6.602 ± 1.522
5.314 ± 0.055
2.738 ± 0.225
4.348 ± 0.639
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here