Microbacterium phage Busephilis

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Quhwahvirus; unclassified Quhwahvirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EM32|A0A4Y6EM32_9CAUD Uncharacterized protein OS=Microbacterium phage Busephilis OX=2588508 GN=72 PE=4 SV=1
MM1 pKa = 7.6AGPYY5 pKa = 9.54KK6 pKa = 10.57VYY8 pKa = 10.52NDD10 pKa = 4.43HH11 pKa = 7.19GDD13 pKa = 4.5LIAGDD18 pKa = 4.34PSLDD22 pKa = 3.92DD23 pKa = 4.34LLKK26 pKa = 10.35ATARR30 pKa = 11.84DD31 pKa = 3.22ASGYY35 pKa = 10.11IEE37 pKa = 5.15DD38 pKa = 4.27ADD40 pKa = 4.16GEE42 pKa = 4.77VVYY45 pKa = 10.89GDD47 pKa = 3.95RR48 pKa = 11.84PAGG51 pKa = 3.52

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EJZ6|A0A4Y6EJZ6_9CAUD Tail assembly chaperone OS=Microbacterium phage Busephilis OX=2588508 GN=33 PE=4 SV=1
MM1 pKa = 8.27IMGCACNKK9 pKa = 9.67QKK11 pKa = 11.41SNGLAAKK18 pKa = 9.89RR19 pKa = 11.84EE20 pKa = 4.13EE21 pKa = 4.39SATTSTSAKK30 pKa = 9.8SDD32 pKa = 3.58TEE34 pKa = 4.1SRR36 pKa = 11.84QATTASRR43 pKa = 11.84LSAPTSRR50 pKa = 11.84RR51 pKa = 11.84QSFALEE57 pKa = 4.12SGGRR61 pKa = 11.84TASFGSRR68 pKa = 11.84LEE70 pKa = 3.93RR71 pKa = 11.84DD72 pKa = 3.0AAAARR77 pKa = 11.84SRR79 pKa = 11.84GG80 pKa = 3.52

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

16851

41

955

189.3

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.106 ± 0.459

0.926 ± 0.156

6.712 ± 0.401

6.445 ± 0.409

2.504 ± 0.138

8.629 ± 0.341

2.0 ± 0.207

4.439 ± 0.279

2.516 ± 0.216

7.483 ± 0.408

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.128

2.486 ± 0.152

6.089 ± 0.299

3.175 ± 0.153

6.991 ± 0.437

5.839 ± 0.289

7.068 ± 0.301

7.537 ± 0.209

1.887 ± 0.159

2.676 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski