Flavobacterium phage FPSV-S27

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M2VC07|A0A6M2VC07_9CAUD Uncharacterized protein OS=Flavobacterium phage FPSV-S27 OX=2575349 PE=4 SV=1
MM1 pKa = 7.58AKK3 pKa = 10.15KK4 pKa = 10.4SIAILKK10 pKa = 9.69QYY12 pKa = 10.34YY13 pKa = 8.5LTGLRR18 pKa = 11.84PTQQNYY24 pKa = 10.24HH25 pKa = 6.76DD26 pKa = 4.17WLDD29 pKa = 3.87SFLHH33 pKa = 6.55KK34 pKa = 10.61DD35 pKa = 3.47DD36 pKa = 4.54GVLITSIVSDD46 pKa = 3.85PLIGNVTINYY56 pKa = 7.7TSGDD60 pKa = 3.61PFIFNVTPTSVPISAVTGLQDD81 pKa = 3.33VLNNFVLQQNGYY93 pKa = 10.09GLTQNNFTNEE103 pKa = 3.75DD104 pKa = 3.72VILLNEE110 pKa = 4.69LGTYY114 pKa = 10.44LDD116 pKa = 3.81YY117 pKa = 11.27LQNYY121 pKa = 9.24NGGLSVKK128 pKa = 10.63NIFIPSNLVLDD139 pKa = 4.02SAGADD144 pKa = 2.88IEE146 pKa = 4.92GFLSEE151 pKa = 4.83IEE153 pKa = 4.02PSFSINRR160 pKa = 11.84GQLLTVKK167 pKa = 10.1YY168 pKa = 10.46NRR170 pKa = 11.84AEE172 pKa = 4.05LSDD175 pKa = 3.78GSSALVHH182 pKa = 6.29CNAVFALPAGKK193 pKa = 10.38YY194 pKa = 9.93GFGFGDD200 pKa = 3.6VYY202 pKa = 11.18GSTVFDD208 pKa = 3.48SYY210 pKa = 11.28EE211 pKa = 3.86FKK213 pKa = 11.06LRR215 pKa = 11.84DD216 pKa = 3.53SAVVLEE222 pKa = 4.65DD223 pKa = 3.72EE224 pKa = 4.52YY225 pKa = 11.74SIFGLNAVDD234 pKa = 3.29IQGNLLTTSVQEE246 pKa = 4.29QFNQAAYY253 pKa = 10.07KK254 pKa = 10.33NITSIYY260 pKa = 8.9GKK262 pKa = 9.32PAALRR267 pKa = 11.84GRR269 pKa = 11.84DD270 pKa = 3.58FTNGYY275 pKa = 8.19SAQWNDD281 pKa = 3.7NTPMSWINDD290 pKa = 3.56EE291 pKa = 4.43IYY293 pKa = 10.54RR294 pKa = 11.84ISTNVNFSQLQDD306 pKa = 3.45GFSVICQIEE315 pKa = 4.24NQDD318 pKa = 4.54PITPSIMRR326 pKa = 11.84RR327 pKa = 11.84FYY329 pKa = 11.17LALNKK334 pKa = 10.4AQGSCVFSCWLEE346 pKa = 3.86EE347 pKa = 4.32VQSNTYY353 pKa = 10.19LKK355 pKa = 10.28RR356 pKa = 11.84KK357 pKa = 9.84KK358 pKa = 10.68FEE360 pKa = 4.01TDD362 pKa = 2.55ATNIPEE368 pKa = 4.36NFFKK372 pKa = 10.5IDD374 pKa = 3.35VEE376 pKa = 4.74VIINDD381 pKa = 4.21LAANNFNCLFNIDD394 pKa = 3.88GASFSLTQGNVTFTDD409 pKa = 3.73YY410 pKa = 11.36SGGYY414 pKa = 7.87GTTNYY419 pKa = 8.38PQNNRR424 pKa = 11.84ICWGNYY430 pKa = 7.89YY431 pKa = 9.29PAKK434 pKa = 10.16ILMSSFYY441 pKa = 10.4YY442 pKa = 10.01QINGIEE448 pKa = 4.4RR449 pKa = 11.84NFSLDD454 pKa = 3.27QPYY457 pKa = 10.27IPYY460 pKa = 8.81ATTDD464 pKa = 3.52GVFMNISPNNDD475 pKa = 3.31NFWDD479 pKa = 4.01LSKK482 pKa = 10.08TSSLYY487 pKa = 10.63VKK489 pKa = 10.04PSSDD493 pKa = 3.8VIRR496 pKa = 11.84ALDD499 pKa = 3.6FNDD502 pKa = 2.62IHH504 pKa = 7.37ALNNTEE510 pKa = 4.43VFDD513 pKa = 4.69LSMYY517 pKa = 9.37MSPNWRR523 pKa = 11.84GKK525 pKa = 10.66VFGQMNNGNLRR536 pKa = 11.84VFITGSYY543 pKa = 10.08KK544 pKa = 10.57GDD546 pKa = 3.32KK547 pKa = 9.82TDD549 pKa = 3.71LNTYY553 pKa = 10.06ILLDD557 pKa = 4.58KK558 pKa = 10.83IPEE561 pKa = 3.96FLFNRR566 pKa = 11.84DD567 pKa = 3.5YY568 pKa = 10.43QTSKK572 pKa = 10.92CLYY575 pKa = 8.32LQNIKK580 pKa = 10.63RR581 pKa = 11.84PTDD584 pKa = 3.73TILGAAVMGIEE595 pKa = 4.26NNLKK599 pKa = 10.12IKK601 pKa = 10.67VNNFDD606 pKa = 3.62INGFEE611 pKa = 4.15IKK613 pKa = 8.69EE614 pKa = 4.3TLVTFDD620 pKa = 4.83LLNN623 pKa = 3.79

Molecular weight: 70.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M2VBX2|A0A6M2VBX2_9CAUD Phage_Mu_F domain-containing protein OS=Flavobacterium phage FPSV-S27 OX=2575349 PE=4 SV=1
MM1 pKa = 6.93MSFRR5 pKa = 11.84FKK7 pKa = 11.03EE8 pKa = 3.82KK9 pKa = 9.92IAAFKK14 pKa = 10.73AMKK17 pKa = 8.62RR18 pKa = 11.84TLPIVVANAAKK29 pKa = 10.3NHH31 pKa = 5.72FLEE34 pKa = 5.01GFRR37 pKa = 11.84KK38 pKa = 10.33GGFTDD43 pKa = 3.91EE44 pKa = 6.05SFDD47 pKa = 3.33PWVKK51 pKa = 10.55RR52 pKa = 11.84KK53 pKa = 10.09AKK55 pKa = 10.08AKK57 pKa = 9.77RR58 pKa = 11.84NAGRR62 pKa = 11.84ALLVYY67 pKa = 9.88KK68 pKa = 10.45GHH70 pKa = 6.92LRR72 pKa = 11.84RR73 pKa = 11.84SIRR76 pKa = 11.84VISASFNKK84 pKa = 9.93IEE86 pKa = 4.2VGSTGTKK93 pKa = 8.52YY94 pKa = 10.82ASRR97 pKa = 11.84HH98 pKa = 4.31NQGLDD103 pKa = 3.36GMPKK107 pKa = 9.46RR108 pKa = 11.84QFIGKK113 pKa = 9.45SGQLHH118 pKa = 5.93KK119 pKa = 10.49KK120 pKa = 8.52ISKK123 pKa = 10.12IIQNKK128 pKa = 9.58IKK130 pKa = 10.69DD131 pKa = 3.7SLLL134 pKa = 3.7

Molecular weight: 15.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

7565

64

623

280.2

31.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.31 ± 0.784

0.833 ± 0.145

6.173 ± 0.291

6.623 ± 0.465

5.327 ± 0.404

6.557 ± 0.446

1.058 ± 0.107

7.971 ± 0.25

8.367 ± 0.63

8.301 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.56 ± 0.219

6.821 ± 0.484

2.895 ± 0.209

3.688 ± 0.195

3.437 ± 0.271

5.843 ± 0.311

5.988 ± 0.265

6.557 ± 0.26

1.058 ± 0.114

3.635 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski