Bacillus phage YungSlug

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; unclassified Spounavirinae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D3V2R0|A0A7D3V2R0_9CAUD Uncharacterized protein OS=Bacillus phage YungSlug OX=2736257 GN=YUNGSLUG_12 PE=4 SV=1
MM1 pKa = 7.35IAWFLFVVGFVFAITLLGNNNTWFLHH27 pKa = 5.17FCSAVGMLFFVIGYY41 pKa = 9.72LKK43 pKa = 10.82DD44 pKa = 3.58SDD46 pKa = 4.53LMTTIWPIFIPFMFFSIITNLFWEE70 pKa = 4.9EE71 pKa = 3.91VV72 pKa = 3.38

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D3QRD3|A0A7D3QRD3_9CAUD Uncharacterized protein OS=Bacillus phage YungSlug OX=2736257 GN=YUNGSLUG_164 PE=4 SV=1
MM1 pKa = 7.17MSYY4 pKa = 10.04FYY6 pKa = 10.31RR7 pKa = 11.84QKK9 pKa = 10.43EE10 pKa = 3.79RR11 pKa = 11.84AGRR14 pKa = 11.84FSSPPPITQEE24 pKa = 4.51RR25 pKa = 11.84IAKK28 pKa = 7.0TKK30 pKa = 10.99NKK32 pKa = 9.65IQVGG36 pKa = 3.76

Molecular weight: 4.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

227

0

227

46458

29

2883

204.7

23.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.917 ± 0.197

0.902 ± 0.078

6.249 ± 0.135

8.681 ± 0.33

4.219 ± 0.124

6.729 ± 0.264

1.649 ± 0.095

6.042 ± 0.126

9.46 ± 0.285

8.007 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.962 ± 0.114

5.351 ± 0.144

3.188 ± 0.13

3.181 ± 0.111

4.314 ± 0.139

5.284 ± 0.157

5.794 ± 0.279

6.836 ± 0.166

1.367 ± 0.073

3.868 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski