Clostridium sp. CAG:127

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5PC08|R5PC08_9CLOT Capsular biosynthesis protein OS=Clostridium sp. CAG:127 OX=1262774 GN=BN482_00830 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.22VCEE5 pKa = 4.08MCGAQNDD12 pKa = 4.23DD13 pKa = 3.81VFTKK17 pKa = 10.66CSVCGADD24 pKa = 3.79LPSMNSQDD32 pKa = 3.74GDD34 pKa = 3.78DD35 pKa = 4.32EE36 pKa = 4.49KK37 pKa = 10.75TVLIDD42 pKa = 3.8PDD44 pKa = 3.5QAPAQPSGLSLKK56 pKa = 9.72TDD58 pKa = 3.31TSTYY62 pKa = 9.05GQADD66 pKa = 3.69AGQTNPQPAAPQFGGMGQPQMNGMGQPQMNGMGQPGMNGMPQQGMPGQAPYY117 pKa = 9.93GQPGFGGPVPAQPAKK132 pKa = 9.81PKK134 pKa = 9.91KK135 pKa = 10.06GLIIGIIAAAVVVLAVAFVLIFKK158 pKa = 10.52GGFGAKK164 pKa = 9.72GGASSPEE171 pKa = 3.51AVAQQFIEE179 pKa = 4.11AMNDD183 pKa = 2.93QDD185 pKa = 4.0VDD187 pKa = 3.96KK188 pKa = 10.38MAALCPPFLDD198 pKa = 4.74PGQEE202 pKa = 4.45DD203 pKa = 3.92IEE205 pKa = 5.05DD206 pKa = 3.85MLDD209 pKa = 3.32SYY211 pKa = 11.55SGYY214 pKa = 10.84DD215 pKa = 3.06VTFKK219 pKa = 11.54YY220 pKa = 10.46EE221 pKa = 4.14GVEE224 pKa = 4.12SKK226 pKa = 10.9EE227 pKa = 4.37MYY229 pKa = 10.69DD230 pKa = 5.54SDD232 pKa = 5.64DD233 pKa = 4.83LDD235 pKa = 4.88DD236 pKa = 5.15LEE238 pKa = 5.53EE239 pKa = 5.72DD240 pKa = 3.24ISDD243 pKa = 4.07YY244 pKa = 11.46ADD246 pKa = 3.37KK247 pKa = 10.63TIKK250 pKa = 10.76LQDD253 pKa = 3.56ACDD256 pKa = 3.86LEE258 pKa = 5.24FSFNVAVTMSGEE270 pKa = 4.42TYY272 pKa = 9.96DD273 pKa = 3.86QSSTYY278 pKa = 10.38PITCIKK284 pKa = 10.81YY285 pKa = 8.6KK286 pKa = 10.72GNWYY290 pKa = 10.2LYY292 pKa = 9.61EE293 pKa = 4.06

Molecular weight: 31.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5NU32|R5NU32_9CLOT Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Clostridium sp. CAG:127 OX=1262774 GN=BN482_00769 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.89VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2463

0

2463

812000

29

2354

329.7

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.779 ± 0.052

1.46 ± 0.021

6.129 ± 0.047

7.393 ± 0.058

3.845 ± 0.034

6.788 ± 0.051

1.74 ± 0.021

7.397 ± 0.041

6.892 ± 0.041

8.54 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.216 ± 0.026

4.496 ± 0.037

3.05 ± 0.026

3.433 ± 0.027

4.095 ± 0.036

5.527 ± 0.039

5.726 ± 0.053

7.191 ± 0.04

0.801 ± 0.016

4.502 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski