Amino acid dipepetide frequency for Clostridium sp. CAG:127

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.547AlaAla: 6.547 ± 0.125	1.326AlaCys: 1.326 ± 0.042	5.006AlaAsp: 5.006 ± 0.087	5.692AlaGlu: 5.692 ± 0.116	3.09AlaPhe: 3.09 ± 0.073	5.903AlaGly: 5.903 ± 0.107	1.182AlaHis: 1.182 ± 0.042	5.599AlaIle: 5.599 ± 0.094	5.72AlaLys: 5.72 ± 0.117	6.606AlaLeu: 6.606 ± 0.094	2.597AlaMet: 2.597 ± 0.056	3.171AlaAsn: 3.171 ± 0.079	2.021AlaPro: 2.021 ± 0.056	2.595AlaGln: 2.595 ± 0.06	2.82AlaArg: 2.82 ± 0.072	4.241AlaSer: 4.241 ± 0.086	3.919AlaThr: 3.919 ± 0.076	5.766AlaVal: 5.766 ± 0.09	0.667AlaTrp: 0.667 ± 0.032	3.319AlaTyr: 3.319 ± 0.075	0.0AlaXaa: 0.0 ± 0.0
Cys	1.025CysAla: 1.025 ± 0.034	0.239CysCys: 0.239 ± 0.016	0.887CysAsp: 0.887 ± 0.033	0.932CysGlu: 0.932 ± 0.032	0.586CysPhe: 0.586 ± 0.028	1.382CysGly: 1.382 ± 0.043	0.256CysHis: 0.256 ± 0.017	1.126CysIle: 1.126 ± 0.034	0.941CysLys: 0.941 ± 0.033	1.187CysLeu: 1.187 ± 0.044	0.557CysMet: 0.557 ± 0.029	0.576CysAsn: 0.576 ± 0.028	0.525CysPro: 0.525 ± 0.031	0.369CysGln: 0.369 ± 0.022	0.618CysArg: 0.618 ± 0.028	0.788CysSer: 0.788 ± 0.036	0.751CysThr: 0.751 ± 0.032	1.143CysVal: 1.143 ± 0.038	0.09CysTrp: 0.09 ± 0.011	0.627CysTyr: 0.627 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	5.632AspAla: 5.632 ± 0.093	0.791AspCys: 0.791 ± 0.031	3.621AspAsp: 3.621 ± 0.087	4.877AspGlu: 4.877 ± 0.078	2.472AspPhe: 2.472 ± 0.063	4.693AspGly: 4.693 ± 0.101	0.881AspHis: 0.881 ± 0.028	4.895AspIle: 4.895 ± 0.086	4.052AspLys: 4.052 ± 0.078	4.41AspLeu: 4.41 ± 0.077	2.16AspMet: 2.16 ± 0.056	2.371AspAsn: 2.371 ± 0.059	1.571AspPro: 1.571 ± 0.039	1.4AspGln: 1.4 ± 0.042	2.282AspArg: 2.282 ± 0.06	3.15AspSer: 3.15 ± 0.064	3.688AspThr: 3.688 ± 0.079	4.576AspVal: 4.576 ± 0.087	0.542AspTrp: 0.542 ± 0.031	3.224AspTyr: 3.224 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	5.794GluAla: 5.794 ± 0.126	0.734GluCys: 0.734 ± 0.029	4.563GluAsp: 4.563 ± 0.078	6.746GluGlu: 6.746 ± 0.131	2.458GluPhe: 2.458 ± 0.065	3.881GluGly: 3.881 ± 0.081	1.486GluHis: 1.486 ± 0.043	5.649GluIle: 5.649 ± 0.091	6.276GluLys: 6.276 ± 0.104	6.41GluLeu: 6.41 ± 0.104	2.32GluMet: 2.32 ± 0.052	4.095GluAsn: 4.095 ± 0.073	1.794GluPro: 1.794 ± 0.056	3.266GluGln: 3.266 ± 0.076	2.95GluArg: 2.95 ± 0.067	3.435GluSer: 3.435 ± 0.078	4.041GluThr: 4.041 ± 0.073	4.374GluVal: 4.374 ± 0.079	0.478GluTrp: 0.478 ± 0.026	3.18GluTyr: 3.18 ± 0.068	0.0GluXaa: 0.0 ± 0.0
Phe	3.193PheAla: 3.193 ± 0.068	0.659PheCys: 0.659 ± 0.024	2.619PheAsp: 2.619 ± 0.057	2.52PheGlu: 2.52 ± 0.062	1.619PhePhe: 1.619 ± 0.053	2.845PheGly: 2.845 ± 0.064	0.787PheHis: 0.787 ± 0.031	2.628PheIle: 2.628 ± 0.059	1.847PheLys: 1.847 ± 0.052	3.539PheLeu: 3.539 ± 0.076	1.145PheMet: 1.145 ± 0.033	1.459PheAsn: 1.459 ± 0.042	1.238PhePro: 1.238 ± 0.043	1.07PheGln: 1.07 ± 0.035	1.511PheArg: 1.511 ± 0.045	2.501PheSer: 2.501 ± 0.062	2.284PheThr: 2.284 ± 0.057	2.998PheVal: 2.998 ± 0.066	0.324PheTrp: 0.324 ± 0.023	1.666PheTyr: 1.666 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.821GlyAla: 4.821 ± 0.098	1.163GlyCys: 1.163 ± 0.04	3.765GlyAsp: 3.765 ± 0.071	4.351GlyGlu: 4.351 ± 0.081	2.813GlyPhe: 2.813 ± 0.063	4.345GlyGly: 4.345 ± 0.09	1.139GlyHis: 1.139 ± 0.045	5.89GlyIle: 5.89 ± 0.096	5.24GlyLys: 5.24 ± 0.08	5.214GlyLeu: 5.214 ± 0.086	2.458GlyMet: 2.458 ± 0.058	3.259GlyAsn: 3.259 ± 0.068	1.105GlyPro: 1.105 ± 0.042	2.097GlyGln: 2.097 ± 0.053	2.714GlyArg: 2.714 ± 0.065	3.681GlySer: 3.681 ± 0.068	4.451GlyThr: 4.451 ± 0.1	5.151GlyVal: 5.151 ± 0.092	0.75GlyTrp: 0.75 ± 0.052	3.472GlyTyr: 3.472 ± 0.076	0.0GlyXaa: 0.0 ± 0.0
His	1.388HisAla: 1.388 ± 0.045	0.278HisCys: 0.278 ± 0.02	0.946HisAsp: 0.946 ± 0.033	1.016HisGlu: 1.016 ± 0.042	0.751HisPhe: 0.751 ± 0.031	1.314HisGly: 1.314 ± 0.044	0.368HisHis: 0.368 ± 0.029	1.341HisIle: 1.341 ± 0.041	1.009HisLys: 1.009 ± 0.039	1.397HisLeu: 1.397 ± 0.041	0.597HisMet: 0.597 ± 0.028	0.734HisAsn: 0.734 ± 0.033	0.75HisPro: 0.75 ± 0.03	0.474HisGln: 0.474 ± 0.024	0.744HisArg: 0.744 ± 0.033	0.876HisSer: 0.876 ± 0.027	1.085HisThr: 1.085 ± 0.035	1.426HisVal: 1.426 ± 0.046	0.134HisTrp: 0.134 ± 0.013	0.768HisTyr: 0.768 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.048IleAla: 6.048 ± 0.094	1.401IleCys: 1.401 ± 0.043	4.44IleAsp: 4.44 ± 0.077	4.917IleGlu: 4.917 ± 0.08	2.794IlePhe: 2.794 ± 0.07	5.078IleGly: 5.078 ± 0.081	1.294IleHis: 1.294 ± 0.035	5.08IleIle: 5.08 ± 0.089	4.238IleLys: 4.238 ± 0.075	6.713IleLeu: 6.713 ± 0.105	2.007IleMet: 2.007 ± 0.047	3.047IleAsn: 3.047 ± 0.062	2.954IlePro: 2.954 ± 0.059	2.427IleGln: 2.427 ± 0.063	3.516IleArg: 3.516 ± 0.074	4.586IleSer: 4.586 ± 0.085	4.308IleThr: 4.308 ± 0.073	5.502IleVal: 5.502 ± 0.09	0.587IleTrp: 0.587 ± 0.028	3.028IleTyr: 3.028 ± 0.066	0.0IleXaa: 0.0 ± 0.0
Lys	4.922LysAla: 4.922 ± 0.101	0.633LysCys: 0.633 ± 0.029	4.273LysAsp: 4.273 ± 0.082	6.099LysGlu: 6.099 ± 0.097	1.929LysPhe: 1.929 ± 0.046	3.719LysGly: 3.719 ± 0.074	1.207LysHis: 1.207 ± 0.04	4.716LysIle: 4.716 ± 0.078	6.413LysLys: 6.413 ± 0.127	6.032LysLeu: 6.032 ± 0.103	2.158LysMet: 2.158 ± 0.057	3.74LysAsn: 3.74 ± 0.085	2.047LysPro: 2.047 ± 0.049	2.94LysGln: 2.94 ± 0.068	3.181LysArg: 3.181 ± 0.069	3.262LysSer: 3.262 ± 0.063	3.839LysThr: 3.839 ± 0.068	4.27LysVal: 4.27 ± 0.076	0.536LysTrp: 0.536 ± 0.026	3.006LysTyr: 3.006 ± 0.07	0.001LysXaa: 0.001 ± 0.001
Leu	7.204LeuAla: 7.204 ± 0.106	1.458LeuCys: 1.458 ± 0.049	5.224LeuAsp: 5.224 ± 0.087	5.628LeuGlu: 5.628 ± 0.098	3.594LeuPhe: 3.594 ± 0.087	5.538LeuGly: 5.538 ± 0.084	1.536LeuHis: 1.536 ± 0.049	5.611LeuIle: 5.611 ± 0.094	5.36LeuLys: 5.36 ± 0.09	7.932LeuLeu: 7.932 ± 0.141	2.446LeuMet: 2.446 ± 0.061	3.573LeuAsn: 3.573 ± 0.077	3.163LeuPro: 3.163 ± 0.072	3.037LeuGln: 3.037 ± 0.068	3.687LeuArg: 3.687 ± 0.085	5.672LeuSer: 5.672 ± 0.101	4.925LeuThr: 4.925 ± 0.077	5.796LeuVal: 5.796 ± 0.092	0.587LeuTrp: 0.587 ± 0.024	3.425LeuTyr: 3.425 ± 0.075	0.0LeuXaa: 0.0 ± 0.0
Met	2.413MetAla: 2.413 ± 0.055	0.4MetCys: 0.4 ± 0.024	2.023MetAsp: 2.023 ± 0.05	2.488MetGlu: 2.488 ± 0.058	1.009MetPhe: 1.009 ± 0.038	2.009MetGly: 2.009 ± 0.056	0.554MetHis: 0.554 ± 0.03	2.282MetIle: 2.282 ± 0.054	2.462MetLys: 2.462 ± 0.051	2.869MetLeu: 2.869 ± 0.059	1.059MetMet: 1.059 ± 0.039	1.613MetAsn: 1.613 ± 0.046	1.129MetPro: 1.129 ± 0.038	1.498MetGln: 1.498 ± 0.045	1.415MetArg: 1.415 ± 0.037	1.86MetSer: 1.86 ± 0.049	1.73MetThr: 1.73 ± 0.047	2.006MetVal: 2.006 ± 0.048	0.243MetTrp: 0.243 ± 0.02	1.097MetTyr: 1.097 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.331AsnAla: 3.331 ± 0.067	0.628AsnCys: 0.628 ± 0.029	2.328AsnAsp: 2.328 ± 0.062	2.83AsnGlu: 2.83 ± 0.064	1.516AsnPhe: 1.516 ± 0.05	3.526AsnGly: 3.526 ± 0.077	0.9AsnHis: 0.9 ± 0.033	3.431AsnIle: 3.431 ± 0.074	2.781AsnLys: 2.781 ± 0.069	3.819AsnLeu: 3.819 ± 0.07	1.483AsnMet: 1.483 ± 0.039	2.106AsnAsn: 2.106 ± 0.08	1.995AsnPro: 1.995 ± 0.054	1.755AsnGln: 1.755 ± 0.044	2.048AsnArg: 2.048 ± 0.049	2.371AsnSer: 2.371 ± 0.062	2.405AsnThr: 2.405 ± 0.06	3.209AsnVal: 3.209 ± 0.078	0.389AsnTrp: 0.389 ± 0.023	2.107AsnTyr: 2.107 ± 0.061	0.0AsnXaa: 0.0 ± 0.0
Pro	2.249ProAla: 2.249 ± 0.059	0.429ProCys: 0.429 ± 0.021	2.305ProAsp: 2.305 ± 0.051	3.181ProGlu: 3.181 ± 0.077	1.317ProPhe: 1.317 ± 0.045	2.055ProGly: 2.055 ± 0.051	0.527ProHis: 0.527 ± 0.025	2.071ProIle: 2.071 ± 0.045	1.841ProLys: 1.841 ± 0.047	2.312ProLeu: 2.312 ± 0.056	0.889ProMet: 0.889 ± 0.039	1.201ProAsn: 1.201 ± 0.042	0.611ProPro: 0.611 ± 0.03	1.032ProGln: 1.032 ± 0.034	0.879ProArg: 0.879 ± 0.031	1.515ProSer: 1.515 ± 0.046	1.565ProThr: 1.565 ± 0.058	2.78ProVal: 2.78 ± 0.064	0.28ProTrp: 0.28 ± 0.02	1.464ProTyr: 1.464 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	3.047GlnAla: 3.047 ± 0.069	0.357GlnCys: 0.357 ± 0.021	1.81GlnAsp: 1.81 ± 0.045	2.733GlnGlu: 2.733 ± 0.063	1.17GlnPhe: 1.17 ± 0.036	2.055GlnGly: 2.055 ± 0.052	0.493GlnHis: 0.493 ± 0.025	2.836GlnIle: 2.836 ± 0.055	2.47GlnLys: 2.47 ± 0.062	2.761GlnLeu: 2.761 ± 0.062	1.36GlnMet: 1.36 ± 0.043	1.586GlnAsn: 1.586 ± 0.049	1.052GlnPro: 1.052 ± 0.044	1.257GlnGln: 1.257 ± 0.052	1.227GlnArg: 1.227 ± 0.039	1.813GlnSer: 1.813 ± 0.05	2.224GlnThr: 2.224 ± 0.06	2.248GlnVal: 2.248 ± 0.051	0.241GlnTrp: 0.241 ± 0.017	1.586GlnTyr: 1.586 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	2.766ArgAla: 2.766 ± 0.067	0.555ArgCys: 0.555 ± 0.027	2.236ArgAsp: 2.236 ± 0.049	3.236ArgGlu: 3.236 ± 0.078	1.682ArgPhe: 1.682 ± 0.054	2.22ArgGly: 2.22 ± 0.054	0.717ArgHis: 0.717 ± 0.029	3.28ArgIle: 3.28 ± 0.063	3.267ArgLys: 3.267 ± 0.065	3.676ArgLeu: 3.676 ± 0.082	1.542ArgMet: 1.542 ± 0.047	2.016ArgAsn: 2.016 ± 0.052	1.128ArgPro: 1.128 ± 0.042	1.603ArgGln: 1.603 ± 0.049	2.044ArgArg: 2.044 ± 0.055	1.897ArgSer: 1.897 ± 0.048	2.048ArgThr: 2.048 ± 0.05	2.812ArgVal: 2.812 ± 0.062	0.289ArgTrp: 0.289 ± 0.019	1.933ArgTyr: 1.933 ± 0.06	0.0ArgXaa: 0.0 ± 0.0
Ser	4.123SerAla: 4.123 ± 0.08	0.713SerCys: 0.713 ± 0.03	3.468SerAsp: 3.468 ± 0.088	3.798SerGlu: 3.798 ± 0.075	2.382SerPhe: 2.382 ± 0.062	4.682SerGly: 4.682 ± 0.077	0.932SerHis: 0.932 ± 0.032	3.957SerIle: 3.957 ± 0.073	3.537SerLys: 3.537 ± 0.073	4.627SerLeu: 4.627 ± 0.083	1.92SerMet: 1.92 ± 0.05	2.339SerAsn: 2.339 ± 0.063	1.473SerPro: 1.473 ± 0.049	1.597SerGln: 1.597 ± 0.047	2.238SerArg: 2.238 ± 0.046	3.079SerSer: 3.079 ± 0.076	2.914SerThr: 2.914 ± 0.066	4.297SerVal: 4.297 ± 0.069	0.392SerTrp: 0.392 ± 0.025	2.798SerTyr: 2.798 ± 0.065	0.001SerXaa: 0.001 ± 0.001
Thr	4.211ThrAla: 4.211 ± 0.077	0.765ThrCys: 0.765 ± 0.03	3.631ThrAsp: 3.631 ± 0.079	4.202ThrGlu: 4.202 ± 0.066	2.256ThrPhe: 2.256 ± 0.056	4.531ThrGly: 4.531 ± 0.082	0.946ThrHis: 0.946 ± 0.038	4.39ThrIle: 4.39 ± 0.083	3.511ThrLys: 3.511 ± 0.06	4.82ThrLeu: 4.82 ± 0.081	1.607ThrMet: 1.607 ± 0.045	2.419ThrAsn: 2.419 ± 0.075	2.118ThrPro: 2.118 ± 0.056	1.665ThrGln: 1.665 ± 0.051	1.895ThrArg: 1.895 ± 0.052	3.124ThrSer: 3.124 ± 0.079	3.512ThrThr: 3.512 ± 0.105	4.491ThrVal: 4.491 ± 0.083	0.52ThrTrp: 0.52 ± 0.026	2.65ThrTyr: 2.65 ± 0.073	0.0ThrXaa: 0.0 ± 0.0
Val	5.257ValAla: 5.257 ± 0.088	1.357ValCys: 1.357 ± 0.039	4.488ValAsp: 4.488 ± 0.071	4.869ValGlu: 4.869 ± 0.085	2.869ValPhe: 2.869 ± 0.068	4.334ValGly: 4.334 ± 0.081	1.177ValHis: 1.177 ± 0.04	5.352ValIle: 5.352 ± 0.094	4.624ValLys: 4.624 ± 0.082	6.581ValLeu: 6.581 ± 0.106	2.151ValMet: 2.151 ± 0.063	3.069ValAsn: 3.069 ± 0.065	2.31ValPro: 2.31 ± 0.055	2.308ValGln: 2.308 ± 0.058	2.882ValArg: 2.882 ± 0.067	4.59ValSer: 4.59 ± 0.085	4.531ValThr: 4.531 ± 0.086	5.445ValVal: 5.445 ± 0.104	0.563ValTrp: 0.563 ± 0.025	3.148ValTyr: 3.148 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	0.456TrpAla: 0.456 ± 0.025	0.116TrpCys: 0.116 ± 0.012	0.472TrpAsp: 0.472 ± 0.025	0.523TrpGlu: 0.523 ± 0.024	0.368TrpPhe: 0.368 ± 0.019	0.482TrpGly: 0.482 ± 0.026	0.16TrpHis: 0.16 ± 0.014	0.56TrpIle: 0.56 ± 0.028	0.661TrpLys: 0.661 ± 0.03	0.844TrpLeu: 0.844 ± 0.038	0.352TrpMet: 0.352 ± 0.022	0.464TrpAsn: 0.464 ± 0.027	0.14TrpPro: 0.14 ± 0.015	0.346TrpGln: 0.346 ± 0.025	0.302TrpArg: 0.302 ± 0.019	0.427TrpSer: 0.427 ± 0.025	0.383TrpThr: 0.383 ± 0.024	0.52TrpVal: 0.52 ± 0.025	0.079TrpTrp: 0.079 ± 0.009	0.353TrpTyr: 0.353 ± 0.02	0.001TrpXaa: 0.001 ± 0.001
Tyr	3.36TyrAla: 3.36 ± 0.065	0.601TyrCys: 0.601 ± 0.027	3.184TyrAsp: 3.184 ± 0.075	3.291TyrGlu: 3.291 ± 0.072	1.778TyrPhe: 1.778 ± 0.047	3.288TyrGly: 3.288 ± 0.075	0.853TyrHis: 0.853 ± 0.034	3.273TyrIle: 3.273 ± 0.071	2.672TyrLys: 2.672 ± 0.058	3.645TyrLeu: 3.645 ± 0.074	1.349TyrMet: 1.349 ± 0.035	2.121TyrAsn: 2.121 ± 0.055	1.378TyrPro: 1.378 ± 0.042	1.589TyrGln: 1.589 ± 0.043	1.996TyrArg: 1.996 ± 0.052	2.398TyrSer: 2.398 ± 0.05	2.661TyrThr: 2.661 ± 0.071	3.097TyrVal: 3.097 ± 0.071	0.319TyrTrp: 0.319 ± 0.02	2.17TyrTyr: 2.17 ± 0.066	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.003

Statistics based on 2463 proteins (812000 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---