Candidatus Sodalis pierantonius str. SOPE
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2189 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0HSG0|W0HSG0_9GAMM Ribosomal RNA large subunit methyltransferase J OS=Candidatus Sodalis pierantonius str. SOPE OX=2342 GN=yhiR PE=3 SV=1
MM1 pKa = 7.42 SNVPTEE7 pKa = 4.39 LKK9 pKa = 9.44 YY10 pKa = 9.08 TASHH14 pKa = 5.55 EE15 pKa = 4.34 WVLNEE20 pKa = 4.02 GDD22 pKa = 3.48 GVYY25 pKa = 10.52 CVGITEE31 pKa = 4.61 HH32 pKa = 6.39 AQEE35 pKa = 4.67 LLGDD39 pKa = 4.16 MVFMDD44 pKa = 4.87 LPEE47 pKa = 4.74 VGASFSAGEE56 pKa = 4.12 DD57 pKa = 3.55 CAVAEE62 pKa = 4.4 SVKK65 pKa = 10.54 AASDD69 pKa = 3.51 IYY71 pKa = 11.37 APISGEE77 pKa = 3.64 IVAVNDD83 pKa = 4.54 DD84 pKa = 4.24 LDD86 pKa = 4.14 GSPEE90 pKa = 4.2 LVNSAPYY97 pKa = 9.09 TDD99 pKa = 3.43 GWLFKK104 pKa = 10.39 IKK106 pKa = 10.49 SADD109 pKa = 3.39 EE110 pKa = 4.51 SEE112 pKa = 4.36 VNEE115 pKa = 4.53 LLDD118 pKa = 3.76 AAAYY122 pKa = 8.65 QAAIDD127 pKa = 4.45 EE128 pKa = 4.4 EE129 pKa = 5.26 DD130 pKa = 3.47 EE131 pKa = 4.2
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.815
IPC2_protein 3.592
IPC_protein 3.554
Toseland 3.363
ProMoST 3.694
Dawson 3.541
Bjellqvist 3.745
Wikipedia 3.465
Rodwell 3.389
Grimsley 3.274
Solomon 3.516
Lehninger 3.478
Nozaki 3.668
DTASelect 3.834
Thurlkill 3.414
EMBOSS 3.465
Sillero 3.668
Patrickios 0.54
IPC_peptide 3.516
IPC2_peptide 3.656
IPC2.peptide.svr19 3.702
Protein with the highest isoelectric point:
>tr|W0HMJ4|W0HMJ4_9GAMM ATP-dependent 6-phosphofructokinase OS=Candidatus Sodalis pierantonius str. SOPE OX=2342 GN=pfkA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.36 GRR39 pKa = 11.84 TRR41 pKa = 11.84 LTVSSKK47 pKa = 11.13
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2189
0
2189
655530
34
1487
299.5
33.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.272 ± 0.056
1.108 ± 0.018
5.374 ± 0.041
5.461 ± 0.056
3.363 ± 0.037
7.232 ± 0.045
2.46 ± 0.026
5.473 ± 0.042
4.164 ± 0.047
11.202 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.736 ± 0.023
3.35 ± 0.033
4.498 ± 0.032
4.613 ± 0.041
6.771 ± 0.047
5.521 ± 0.032
5.228 ± 0.032
6.96 ± 0.038
1.437 ± 0.023
2.777 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here