Amino acid dipepetide frequency for Thermoflexus hugenholtzii JAD2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.41AlaAla: 14.41 ± 0.201	1.013AlaCys: 1.013 ± 0.037	4.575AlaAsp: 4.575 ± 0.074	7.798AlaGlu: 7.798 ± 0.117	4.937AlaPhe: 4.937 ± 0.076	9.243AlaGly: 9.243 ± 0.092	2.343AlaHis: 2.343 ± 0.049	5.26AlaIle: 5.26 ± 0.08	1.561AlaLys: 1.561 ± 0.057	17.161AlaLeu: 17.161 ± 0.218	3.041AlaMet: 3.041 ± 0.057	1.324AlaAsn: 1.324 ± 0.035	5.899AlaPro: 5.899 ± 0.087	3.77AlaGln: 3.77 ± 0.065	11.605AlaArg: 11.605 ± 0.131	3.971AlaSer: 3.971 ± 0.065	4.213AlaThr: 4.213 ± 0.071	8.585AlaVal: 8.585 ± 0.105	2.605AlaTrp: 2.605 ± 0.068	3.076AlaTyr: 3.076 ± 0.063	0.001AlaXaa: 0.001 ± 0.001
Cys	0.839CysAla: 0.839 ± 0.035	0.095CysCys: 0.095 ± 0.011	0.319CysAsp: 0.319 ± 0.02	0.392CysGlu: 0.392 ± 0.021	0.234CysPhe: 0.234 ± 0.018	0.931CysGly: 0.931 ± 0.037	0.208CysHis: 0.208 ± 0.018	0.3CysIle: 0.3 ± 0.018	0.113CysLys: 0.113 ± 0.012	0.661CysLeu: 0.661 ± 0.027	0.114CysMet: 0.114 ± 0.011	0.156CysAsn: 0.156 ± 0.013	0.712CysPro: 0.712 ± 0.032	0.239CysGln: 0.239 ± 0.017	0.662CysArg: 0.662 ± 0.026	0.271CysSer: 0.271 ± 0.021	0.346CysThr: 0.346 ± 0.021	0.453CysVal: 0.453 ± 0.024	0.129CysTrp: 0.129 ± 0.011	0.22CysTyr: 0.22 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.953AspAla: 3.953 ± 0.07	0.24AspCys: 0.24 ± 0.017	1.331AspAsp: 1.331 ± 0.043	2.671AspGlu: 2.671 ± 0.06	1.6AspPhe: 1.6 ± 0.04	3.791AspGly: 3.791 ± 0.073	0.909AspHis: 0.909 ± 0.033	1.649AspIle: 1.649 ± 0.042	0.541AspLys: 0.541 ± 0.028	6.295AspLeu: 6.295 ± 0.085	0.534AspMet: 0.534 ± 0.021	0.531AspAsn: 0.531 ± 0.026	5.011AspPro: 5.011 ± 0.082	1.155AspGln: 1.155 ± 0.037	4.057AspArg: 4.057 ± 0.07	1.103AspSer: 1.103 ± 0.036	1.427AspThr: 1.427 ± 0.043	2.968AspVal: 2.968 ± 0.068	0.905AspTrp: 0.905 ± 0.031	1.235AspTyr: 1.235 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	11.3GluAla: 11.3 ± 0.163	0.293GluCys: 0.293 ± 0.019	3.189GluAsp: 3.189 ± 0.06	6.664GluGlu: 6.664 ± 0.127	1.627GluPhe: 1.627 ± 0.043	6.476GluGly: 6.476 ± 0.08	1.171GluHis: 1.171 ± 0.037	4.333GluIle: 4.333 ± 0.08	1.589GluLys: 1.589 ± 0.056	6.017GluLeu: 6.017 ± 0.099	1.482GluMet: 1.482 ± 0.037	1.204GluAsn: 1.204 ± 0.032	3.783GluPro: 3.783 ± 0.058	2.117GluGln: 2.117 ± 0.052	7.34GluArg: 7.34 ± 0.111	1.906GluSer: 1.906 ± 0.049	3.156GluThr: 3.156 ± 0.052	5.768GluVal: 5.768 ± 0.078	1.284GluTrp: 1.284 ± 0.037	1.015GluTyr: 1.015 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.572PheAla: 3.572 ± 0.059	0.354PheCys: 0.354 ± 0.019	1.625PheAsp: 1.625 ± 0.043	2.149PheGlu: 2.149 ± 0.047	1.432PhePhe: 1.432 ± 0.044	3.134PheGly: 3.134 ± 0.061	0.83PheHis: 0.83 ± 0.028	1.542PheIle: 1.542 ± 0.041	0.446PheLys: 0.446 ± 0.019	4.385PheLeu: 4.385 ± 0.076	0.574PheMet: 0.574 ± 0.024	0.715PheAsn: 0.715 ± 0.03	2.408PhePro: 2.408 ± 0.052	1.13PheGln: 1.13 ± 0.036	3.127PheArg: 3.127 ± 0.054	1.519PheSer: 1.519 ± 0.044	1.783PheThr: 1.783 ± 0.047	2.272PheVal: 2.272 ± 0.054	0.728PheTrp: 0.728 ± 0.028	1.223PheTyr: 1.223 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	8.241GlyAla: 8.241 ± 0.1	0.744GlyCys: 0.744 ± 0.031	3.451GlyAsp: 3.451 ± 0.059	5.694GlyGlu: 5.694 ± 0.091	3.359GlyPhe: 3.359 ± 0.068	6.758GlyGly: 6.758 ± 0.105	1.691GlyHis: 1.691 ± 0.045	4.869GlyIle: 4.869 ± 0.079	2.074GlyLys: 2.074 ± 0.058	10.714GlyLeu: 10.714 ± 0.125	2.516GlyMet: 2.516 ± 0.048	1.441GlyAsn: 1.441 ± 0.047	4.774GlyPro: 4.774 ± 0.076	2.575GlyGln: 2.575 ± 0.051	7.66GlyArg: 7.66 ± 0.093	3.572GlySer: 3.572 ± 0.067	3.977GlyThr: 3.977 ± 0.071	6.439GlyVal: 6.439 ± 0.088	2.393GlyTrp: 2.393 ± 0.053	2.442GlyTyr: 2.442 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.963HisAla: 1.963 ± 0.049	0.18HisCys: 0.18 ± 0.017	0.689HisAsp: 0.689 ± 0.029	1.025HisGlu: 1.025 ± 0.032	0.603HisPhe: 0.603 ± 0.021	1.762HisGly: 1.762 ± 0.045	0.574HisHis: 0.574 ± 0.029	0.984HisIle: 0.984 ± 0.033	0.289HisLys: 0.289 ± 0.019	2.561HisLeu: 2.561 ± 0.051	0.31HisMet: 0.31 ± 0.018	0.405HisAsn: 0.405 ± 0.019	2.153HisPro: 2.153 ± 0.056	0.59HisGln: 0.59 ± 0.024	2.077HisArg: 2.077 ± 0.046	0.618HisSer: 0.618 ± 0.026	0.839HisThr: 0.839 ± 0.031	1.271HisVal: 1.271 ± 0.038	0.317HisTrp: 0.317 ± 0.017	0.549HisTyr: 0.549 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.021IleAla: 6.021 ± 0.078	0.402IleCys: 0.402 ± 0.021	2.019IleAsp: 2.019 ± 0.045	3.179IleGlu: 3.179 ± 0.073	1.57IlePhe: 1.57 ± 0.043	4.706IleGly: 4.706 ± 0.072	1.085IleHis: 1.085 ± 0.037	1.605IleIle: 1.605 ± 0.044	0.672IleLys: 0.672 ± 0.032	6.3IleLeu: 6.3 ± 0.088	0.612IleMet: 0.612 ± 0.026	0.834IleAsn: 0.834 ± 0.035	4.032IlePro: 4.032 ± 0.065	1.492IleGln: 1.492 ± 0.038	5.245IleArg: 5.245 ± 0.065	1.974IleSer: 1.974 ± 0.042	2.327IleThr: 2.327 ± 0.056	3.51IleVal: 3.51 ± 0.065	0.848IleTrp: 0.848 ± 0.029	1.32IleTyr: 1.32 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	2.43LysAla: 2.43 ± 0.065	0.109LysCys: 0.109 ± 0.011	0.795LysAsp: 0.795 ± 0.033	1.356LysGlu: 1.356 ± 0.044	0.437LysPhe: 0.437 ± 0.023	1.772LysGly: 1.772 ± 0.047	0.276LysHis: 0.276 ± 0.016	0.952LysIle: 0.952 ± 0.035	0.531LysLys: 0.531 ± 0.033	1.296LysLeu: 1.296 ± 0.046	0.377LysMet: 0.377 ± 0.02	0.358LysAsn: 0.358 ± 0.02	1.215LysPro: 1.215 ± 0.04	0.475LysGln: 0.475 ± 0.022	1.411LysArg: 1.411 ± 0.045	0.606LysSer: 0.606 ± 0.026	0.967LysThr: 0.967 ± 0.033	1.487LysVal: 1.487 ± 0.046	0.285LysTrp: 0.285 ± 0.02	0.415LysTyr: 0.415 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	14.376LeuAla: 14.376 ± 0.167	0.828LeuCys: 0.828 ± 0.03	4.864LeuAsp: 4.864 ± 0.082	8.526LeuGlu: 8.526 ± 0.113	4.047LeuPhe: 4.047 ± 0.087	9.921LeuGly: 9.921 ± 0.132	2.286LeuHis: 2.286 ± 0.046	6.287LeuIle: 6.287 ± 0.1	2.318LeuLys: 2.318 ± 0.053	13.774LeuLeu: 13.774 ± 0.185	2.238LeuMet: 2.238 ± 0.05	2.099LeuAsn: 2.099 ± 0.049	7.91LeuPro: 7.91 ± 0.11	4.038LeuGln: 4.038 ± 0.069	11.859LeuArg: 11.859 ± 0.136	5.857LeuSer: 5.857 ± 0.085	5.552LeuThr: 5.552 ± 0.087	7.482LeuVal: 7.482 ± 0.109	2.422LeuTrp: 2.422 ± 0.066	2.998LeuTyr: 2.998 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	3.234MetAla: 3.234 ± 0.063	0.11MetCys: 0.11 ± 0.01	0.96MetAsp: 0.96 ± 0.033	1.578MetGlu: 1.578 ± 0.042	0.542MetPhe: 0.542 ± 0.026	2.111MetGly: 2.111 ± 0.055	0.3MetHis: 0.3 ± 0.018	1.092MetIle: 1.092 ± 0.034	0.533MetLys: 0.533 ± 0.025	2.067MetLeu: 2.067 ± 0.041	0.467MetMet: 0.467 ± 0.024	0.418MetAsn: 0.418 ± 0.021	1.309MetPro: 1.309 ± 0.037	0.551MetGln: 0.551 ± 0.025	2.038MetArg: 2.038 ± 0.042	0.96MetSer: 0.96 ± 0.031	1.022MetThr: 1.022 ± 0.028	1.646MetVal: 1.646 ± 0.035	0.288MetTrp: 0.288 ± 0.016	0.29MetTyr: 0.29 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	1.908AsnAla: 1.908 ± 0.051	0.126AsnCys: 0.126 ± 0.014	0.636AsnAsp: 0.636 ± 0.027	0.775AsnGlu: 0.775 ± 0.029	0.576AsnPhe: 0.576 ± 0.023	1.516AsnGly: 1.516 ± 0.045	0.308AsnHis: 0.308 ± 0.02	0.799AsnIle: 0.799 ± 0.031	0.294AsnLys: 0.294 ± 0.018	2.112AsnLeu: 2.112 ± 0.049	0.302AsnMet: 0.302 ± 0.02	0.352AsnAsn: 0.352 ± 0.025	1.717AsnPro: 1.717 ± 0.046	0.443AsnGln: 0.443 ± 0.023	1.376AsnArg: 1.376 ± 0.042	0.501AsnSer: 0.501 ± 0.026	0.838AsnThr: 0.838 ± 0.037	1.224AsnVal: 1.224 ± 0.042	0.315AsnTrp: 0.315 ± 0.018	0.448AsnTyr: 0.448 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.227ProAla: 7.227 ± 0.097	0.394ProCys: 0.394 ± 0.021	3.968ProAsp: 3.968 ± 0.069	6.737ProGlu: 6.737 ± 0.1	2.375ProPhe: 2.375 ± 0.053	6.096ProGly: 6.096 ± 0.102	1.421ProHis: 1.421 ± 0.045	2.909ProIle: 2.909 ± 0.06	1.003ProLys: 1.003 ± 0.035	7.305ProLeu: 7.305 ± 0.093	1.534ProMet: 1.534 ± 0.039	1.168ProAsn: 1.168 ± 0.041	5.466ProPro: 5.466 ± 0.112	2.02ProGln: 2.02 ± 0.045	4.987ProArg: 4.987 ± 0.074	3.357ProSer: 3.357 ± 0.074	3.643ProThr: 3.643 ± 0.113	5.193ProVal: 5.193 ± 0.071	1.403ProTrp: 1.403 ± 0.037	1.712ProTyr: 1.712 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.888GlnAla: 4.888 ± 0.093	0.161GlnCys: 0.161 ± 0.014	1.139GlnAsp: 1.139 ± 0.036	2.374GlnGlu: 2.374 ± 0.053	0.712GlnPhe: 0.712 ± 0.031	2.597GlnGly: 2.597 ± 0.054	0.514GlnHis: 0.514 ± 0.02	1.759GlnIle: 1.759 ± 0.05	0.606GlnLys: 0.606 ± 0.029	2.495GlnLeu: 2.495 ± 0.06	0.671GlnMet: 0.671 ± 0.027	0.485GlnAsn: 0.485 ± 0.025	2.005GlnPro: 2.005 ± 0.052	1.035GlnGln: 1.035 ± 0.035	2.986GlnArg: 2.986 ± 0.055	0.857GlnSer: 0.857 ± 0.033	1.346GlnThr: 1.346 ± 0.041	2.663GlnVal: 2.663 ± 0.057	0.491GlnTrp: 0.491 ± 0.024	0.462GlnTyr: 0.462 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	10.412ArgAla: 10.412 ± 0.128	0.758ArgCys: 0.758 ± 0.033	3.47ArgAsp: 3.47 ± 0.056	7.111ArgGlu: 7.111 ± 0.087	3.442ArgPhe: 3.442 ± 0.054	6.4ArgGly: 6.4 ± 0.087	1.762ArgHis: 1.762 ± 0.043	5.264ArgIle: 5.264 ± 0.067	1.626ArgLys: 1.626 ± 0.043	11.425ArgLeu: 11.425 ± 0.121	2.56ArgMet: 2.56 ± 0.054	1.46ArgAsn: 1.46 ± 0.045	6.183ArgPro: 6.183 ± 0.089	2.921ArgGln: 2.921 ± 0.059	9.892ArgArg: 9.892 ± 0.139	3.496ArgSer: 3.496 ± 0.065	3.773ArgThr: 3.773 ± 0.06	6.859ArgVal: 6.859 ± 0.09	2.778ArgTrp: 2.778 ± 0.068	2.807ArgTyr: 2.807 ± 0.059	0.001ArgXaa: 0.001 ± 0.001
Ser	4.011SerAla: 4.011 ± 0.065	0.316SerCys: 0.316 ± 0.018	1.549SerAsp: 1.549 ± 0.041	2.214SerGlu: 2.214 ± 0.051	1.456SerPhe: 1.456 ± 0.04	4.037SerGly: 4.037 ± 0.066	0.737SerHis: 0.737 ± 0.027	1.682SerIle: 1.682 ± 0.048	0.598SerLys: 0.598 ± 0.026	4.688SerLeu: 4.688 ± 0.074	0.901SerMet: 0.901 ± 0.034	0.589SerAsn: 0.589 ± 0.028	3.719SerPro: 3.719 ± 0.079	1.005SerGln: 1.005 ± 0.03	3.186SerArg: 3.186 ± 0.06	1.77SerSer: 1.77 ± 0.05	1.721SerThr: 1.721 ± 0.042	2.528SerVal: 2.528 ± 0.054	0.745SerTrp: 0.745 ± 0.028	0.806SerTyr: 0.806 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	4.756ThrAla: 4.756 ± 0.069	0.359ThrCys: 0.359 ± 0.02	1.821ThrAsp: 1.821 ± 0.055	2.323ThrGlu: 2.323 ± 0.052	1.693ThrPhe: 1.693 ± 0.05	4.104ThrGly: 4.104 ± 0.073	0.929ThrHis: 0.929 ± 0.034	1.898ThrIle: 1.898 ± 0.048	0.598ThrLys: 0.598 ± 0.026	6.142ThrLeu: 6.142 ± 0.095	0.902ThrMet: 0.902 ± 0.034	0.703ThrAsn: 0.703 ± 0.035	4.306ThrPro: 4.306 ± 0.108	1.022ThrGln: 1.022 ± 0.034	3.308ThrArg: 3.308 ± 0.064	1.651ThrSer: 1.651 ± 0.046	1.86ThrThr: 1.86 ± 0.06	3.918ThrVal: 3.918 ± 0.072	0.867ThrTrp: 0.867 ± 0.037	1.198ThrTyr: 1.198 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	7.884ValAla: 7.884 ± 0.099	0.579ValCys: 0.579 ± 0.028	3.475ValAsp: 3.475 ± 0.058	5.841ValGlu: 5.841 ± 0.091	2.714ValPhe: 2.714 ± 0.058	5.862ValGly: 5.862 ± 0.083	1.513ValHis: 1.513 ± 0.042	4.159ValIle: 4.159 ± 0.076	1.352ValLys: 1.352 ± 0.043	8.564ValLeu: 8.564 ± 0.098	1.478ValMet: 1.478 ± 0.042	1.351ValAsn: 1.351 ± 0.041	4.445ValPro: 4.445 ± 0.075	2.189ValGln: 2.189 ± 0.046	6.99ValArg: 6.99 ± 0.09	2.637ValSer: 2.637 ± 0.054	3.337ValThr: 3.337 ± 0.07	6.192ValVal: 6.192 ± 0.107	1.366ValTrp: 1.366 ± 0.041	1.943ValTyr: 1.943 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	2.302TrpAla: 2.302 ± 0.056	0.142TrpCys: 0.142 ± 0.011	0.756TrpAsp: 0.756 ± 0.028	1.545TrpGlu: 1.545 ± 0.041	0.644TrpPhe: 0.644 ± 0.027	1.797TrpGly: 1.797 ± 0.055	0.325TrpHis: 0.325 ± 0.019	1.336TrpIle: 1.336 ± 0.034	0.417TrpLys: 0.417 ± 0.023	2.654TrpLeu: 2.654 ± 0.064	0.552TrpMet: 0.552 ± 0.027	0.438TrpAsn: 0.438 ± 0.02	1.25TrpPro: 1.25 ± 0.042	0.675TrpGln: 0.675 ± 0.027	1.955TrpArg: 1.955 ± 0.057	0.878TrpSer: 0.878 ± 0.035	1.002TrpThr: 1.002 ± 0.032	1.557TrpVal: 1.557 ± 0.044	0.49TrpTrp: 0.49 ± 0.027	0.373TrpTyr: 0.373 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.669TyrAla: 2.669 ± 0.047	0.195TyrCys: 0.195 ± 0.014	1.27TyrAsp: 1.27 ± 0.071	1.761TyrGlu: 1.761 ± 0.045	0.946TyrPhe: 0.946 ± 0.029	2.368TyrGly: 2.368 ± 0.048	0.579TyrHis: 0.579 ± 0.022	1.071TyrIle: 1.071 ± 0.04	0.389TyrLys: 0.389 ± 0.019	3.063TyrLeu: 3.063 ± 0.057	0.35TyrMet: 0.35 ± 0.021	0.535TyrAsn: 0.535 ± 0.024	1.621TyrPro: 1.621 ± 0.043	0.803TyrGln: 0.803 ± 0.029	2.639TyrArg: 2.639 ± 0.058	0.754TyrSer: 0.754 ± 0.029	1.229TyrThr: 1.229 ± 0.047	1.856TyrVal: 1.856 ± 0.048	0.434TyrTrp: 0.434 ± 0.023	0.744TyrTyr: 0.744 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.015XaaXaa: 0.015 ± 0.012

Statistics based on 2944 proteins (955847 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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