Amino acid dipepetide frequency for Amycolatopsis sacchari

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.522AlaAla: 20.522 ± 0.14	1.0AlaCys: 1.0 ± 0.019	7.899AlaAsp: 7.899 ± 0.062	9.474AlaGlu: 9.474 ± 0.089	3.674AlaPhe: 3.674 ± 0.044	13.219AlaGly: 13.219 ± 0.094	2.577AlaHis: 2.577 ± 0.035	4.136AlaIle: 4.136 ± 0.05	3.028AlaLys: 3.028 ± 0.043	14.298AlaLeu: 14.298 ± 0.101	2.383AlaMet: 2.383 ± 0.031	2.259AlaAsn: 2.259 ± 0.034	6.287AlaPro: 6.287 ± 0.066	3.757AlaGln: 3.757 ± 0.043	10.094AlaArg: 10.094 ± 0.084	5.727AlaSer: 5.727 ± 0.058	6.923AlaThr: 6.923 ± 0.049	12.236AlaVal: 12.236 ± 0.092	1.778AlaTrp: 1.778 ± 0.028	2.35AlaTyr: 2.35 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	0.982CysAla: 0.982 ± 0.022	0.099CysCys: 0.099 ± 0.007	0.412CysAsp: 0.412 ± 0.014	0.405CysGlu: 0.405 ± 0.014	0.244CysPhe: 0.244 ± 0.011	0.92CysGly: 0.92 ± 0.02	0.189CysHis: 0.189 ± 0.008	0.15CysIle: 0.15 ± 0.007	0.095CysLys: 0.095 ± 0.006	0.75CysLeu: 0.75 ± 0.018	0.088CysMet: 0.088 ± 0.006	0.112CysAsn: 0.112 ± 0.008	0.47CysPro: 0.47 ± 0.016	0.165CysGln: 0.165 ± 0.009	0.558CysArg: 0.558 ± 0.017	0.41CysSer: 0.41 ± 0.015	0.458CysThr: 0.458 ± 0.014	0.637CysVal: 0.637 ± 0.017	0.124CysTrp: 0.124 ± 0.007	0.188CysTyr: 0.188 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.135AspAla: 7.135 ± 0.064	0.375AspCys: 0.375 ± 0.011	3.3AspAsp: 3.3 ± 0.046	4.116AspGlu: 4.116 ± 0.042	1.616AspPhe: 1.616 ± 0.027	5.714AspGly: 5.714 ± 0.064	1.286AspHis: 1.286 ± 0.024	1.634AspIle: 1.634 ± 0.029	1.017AspLys: 1.017 ± 0.023	6.284AspLeu: 6.284 ± 0.05	0.663AspMet: 0.663 ± 0.017	0.953AspAsn: 0.953 ± 0.024	4.338AspPro: 4.338 ± 0.052	1.553AspGln: 1.553 ± 0.03	4.635AspArg: 4.635 ± 0.05	2.391AspSer: 2.391 ± 0.03	2.765AspThr: 2.765 ± 0.035	5.324AspVal: 5.324 ± 0.058	0.949AspTrp: 0.949 ± 0.021	1.199AspTyr: 1.199 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.264GluAla: 7.264 ± 0.075	0.335GluCys: 0.335 ± 0.012	2.906GluAsp: 2.906 ± 0.038	3.373GluGlu: 3.373 ± 0.051	1.787GluPhe: 1.787 ± 0.028	3.927GluGly: 3.927 ± 0.047	1.808GluHis: 1.808 ± 0.034	2.399GluIle: 2.399 ± 0.031	1.288GluLys: 1.288 ± 0.026	7.736GluLeu: 7.736 ± 0.069	0.823GluMet: 0.823 ± 0.019	1.096GluAsn: 1.096 ± 0.024	3.681GluPro: 3.681 ± 0.049	2.452GluGln: 2.452 ± 0.034	5.656GluArg: 5.656 ± 0.059	2.457GluSer: 2.457 ± 0.028	2.859GluThr: 2.859 ± 0.035	5.184GluVal: 5.184 ± 0.051	0.844GluTrp: 0.844 ± 0.02	1.115GluTyr: 1.115 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	4.238PheAla: 4.238 ± 0.045	0.276PheCys: 0.276 ± 0.011	2.143PheAsp: 2.143 ± 0.033	1.55PheGlu: 1.55 ± 0.027	0.943PhePhe: 0.943 ± 0.024	3.44PheGly: 3.44 ± 0.048	0.595PheHis: 0.595 ± 0.016	0.665PheIle: 0.665 ± 0.018	0.411PheLys: 0.411 ± 0.015	2.856PheLeu: 2.856 ± 0.036	0.341PheMet: 0.341 ± 0.013	0.555PheAsn: 0.555 ± 0.017	1.521PhePro: 1.521 ± 0.026	0.677PheGln: 0.677 ± 0.018	1.924PheArg: 1.924 ± 0.029	1.563PheSer: 1.563 ± 0.028	2.077PheThr: 2.077 ± 0.032	2.623PheVal: 2.623 ± 0.032	0.408PheTrp: 0.408 ± 0.014	0.609PheTyr: 0.609 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	10.38GlyAla: 10.38 ± 0.082	0.745GlyCys: 0.745 ± 0.021	4.727GlyAsp: 4.727 ± 0.049	5.573GlyGlu: 5.573 ± 0.053	3.132GlyPhe: 3.132 ± 0.047	8.601GlyGly: 8.601 ± 0.086	2.116GlyHis: 2.116 ± 0.037	3.708GlyIle: 3.708 ± 0.044	2.458GlyLys: 2.458 ± 0.037	9.643GlyLeu: 9.643 ± 0.071	1.997GlyMet: 1.997 ± 0.032	1.881GlyAsn: 1.881 ± 0.037	4.642GlyPro: 4.642 ± 0.048	2.91GlyGln: 2.91 ± 0.047	7.292GlyArg: 7.292 ± 0.056	5.103GlySer: 5.103 ± 0.052	5.777GlyThr: 5.777 ± 0.056	8.152GlyVal: 8.152 ± 0.065	1.782GlyTrp: 1.782 ± 0.033	2.405GlyTyr: 2.405 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.598HisAla: 2.598 ± 0.035	0.208HisCys: 0.208 ± 0.009	1.299HisAsp: 1.299 ± 0.022	1.276HisGlu: 1.276 ± 0.026	0.674HisPhe: 0.674 ± 0.017	2.335HisGly: 2.335 ± 0.038	0.632HisHis: 0.632 ± 0.02	0.597HisIle: 0.597 ± 0.017	0.3HisLys: 0.3 ± 0.011	2.428HisLeu: 2.428 ± 0.031	0.27HisMet: 0.27 ± 0.009	0.395HisAsn: 0.395 ± 0.014	1.728HisPro: 1.728 ± 0.028	0.595HisGln: 0.595 ± 0.017	1.932HisArg: 1.932 ± 0.028	0.963HisSer: 0.963 ± 0.021	1.135HisThr: 1.135 ± 0.023	1.876HisVal: 1.876 ± 0.032	0.356HisTrp: 0.356 ± 0.013	0.521HisTyr: 0.521 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	5.029IleAla: 5.029 ± 0.041	0.281IleCys: 0.281 ± 0.01	2.208IleAsp: 2.208 ± 0.034	2.006IleGlu: 2.006 ± 0.033	0.776IlePhe: 0.776 ± 0.022	3.662IleGly: 3.662 ± 0.045	0.544IleHis: 0.544 ± 0.016	0.877IleIle: 0.877 ± 0.018	0.632IleLys: 0.632 ± 0.016	2.38IleLeu: 2.38 ± 0.033	0.401IleMet: 0.401 ± 0.012	0.703IleAsn: 0.703 ± 0.021	1.816IlePro: 1.816 ± 0.026	0.697IleGln: 0.697 ± 0.018	2.261IleArg: 2.261 ± 0.032	1.786IleSer: 1.786 ± 0.03	2.34IleThr: 2.34 ± 0.034	3.008IleVal: 3.008 ± 0.037	0.357IleTrp: 0.357 ± 0.013	0.581IleTyr: 0.581 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.719LysAla: 2.719 ± 0.043	0.103LysCys: 0.103 ± 0.008	0.993LysAsp: 0.993 ± 0.024	0.962LysGlu: 0.962 ± 0.025	0.503LysPhe: 0.503 ± 0.018	1.481LysGly: 1.481 ± 0.029	0.454LysHis: 0.454 ± 0.015	0.872LysIle: 0.872 ± 0.021	0.543LysLys: 0.543 ± 0.019	2.234LysLeu: 2.234 ± 0.034	0.345LysMet: 0.345 ± 0.013	0.422LysAsn: 0.422 ± 0.014	1.42LysPro: 1.42 ± 0.024	0.717LysGln: 0.717 ± 0.018	1.561LysArg: 1.561 ± 0.025	0.943LysSer: 0.943 ± 0.022	1.171LysThr: 1.171 ± 0.024	1.894LysVal: 1.894 ± 0.033	0.278LysTrp: 0.278 ± 0.011	0.399LysTyr: 0.399 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	16.412LeuAla: 16.412 ± 0.124	0.802LeuCys: 0.802 ± 0.018	6.973LeuAsp: 6.973 ± 0.055	4.952LeuGlu: 4.952 ± 0.06	2.783LeuPhe: 2.783 ± 0.04	9.965LeuGly: 9.965 ± 0.079	2.267LeuHis: 2.267 ± 0.032	3.123LeuIle: 3.123 ± 0.042	1.748LeuLys: 1.748 ± 0.029	11.587LeuLeu: 11.587 ± 0.118	1.406LeuMet: 1.406 ± 0.024	1.79LeuAsn: 1.79 ± 0.029	6.764LeuPro: 6.764 ± 0.049	2.153LeuGln: 2.153 ± 0.03	9.26LeuArg: 9.26 ± 0.078	5.716LeuSer: 5.716 ± 0.042	6.622LeuThr: 6.622 ± 0.054	10.108LeuVal: 10.108 ± 0.083	1.255LeuTrp: 1.255 ± 0.022	1.735LeuTyr: 1.735 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.024MetAla: 2.024 ± 0.03	0.117MetCys: 0.117 ± 0.006	0.749MetAsp: 0.749 ± 0.019	0.566MetGlu: 0.566 ± 0.016	0.469MetPhe: 0.469 ± 0.015	1.176MetGly: 1.176 ± 0.022	0.308MetHis: 0.308 ± 0.013	0.651MetIle: 0.651 ± 0.017	0.382MetLys: 0.382 ± 0.013	1.684MetLeu: 1.684 ± 0.03	0.233MetMet: 0.233 ± 0.011	0.4MetAsn: 0.4 ± 0.014	1.036MetPro: 1.036 ± 0.022	0.37MetGln: 0.37 ± 0.014	1.397MetArg: 1.397 ± 0.027	1.217MetSer: 1.217 ± 0.023	1.493MetThr: 1.493 ± 0.024	1.209MetVal: 1.209 ± 0.025	0.186MetTrp: 0.186 ± 0.008	0.25MetTyr: 0.25 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.385AsnAla: 2.385 ± 0.034	0.152AsnCys: 0.152 ± 0.008	0.951AsnAsp: 0.951 ± 0.021	0.843AsnGlu: 0.843 ± 0.019	0.57AsnPhe: 0.57 ± 0.017	1.83AsnGly: 1.83 ± 0.036	0.405AsnHis: 0.405 ± 0.012	0.615AsnIle: 0.615 ± 0.019	0.343AsnLys: 0.343 ± 0.014	1.99AsnLeu: 1.99 ± 0.032	0.257AsnMet: 0.257 ± 0.01	0.462AsnAsn: 0.462 ± 0.015	1.529AsnPro: 1.529 ± 0.029	0.581AsnGln: 0.581 ± 0.016	1.3AsnArg: 1.3 ± 0.024	0.94AsnSer: 0.94 ± 0.021	1.118AsnThr: 1.118 ± 0.024	1.486AsnVal: 1.486 ± 0.028	0.304AsnTrp: 0.304 ± 0.013	0.456AsnTyr: 0.456 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.165ProAla: 8.165 ± 0.065	0.343ProCys: 0.343 ± 0.015	4.213ProAsp: 4.213 ± 0.05	4.632ProGlu: 4.632 ± 0.052	1.615ProPhe: 1.615 ± 0.028	6.716ProGly: 6.716 ± 0.065	1.273ProHis: 1.273 ± 0.021	1.44ProIle: 1.44 ± 0.023	1.175ProLys: 1.175 ± 0.024	5.405ProLeu: 5.405 ± 0.054	0.959ProMet: 0.959 ± 0.019	1.03ProAsn: 1.03 ± 0.025	3.654ProPro: 3.654 ± 0.066	1.621ProGln: 1.621 ± 0.031	4.025ProArg: 4.025 ± 0.045	3.039ProSer: 3.039 ± 0.04	2.9ProThr: 2.9 ± 0.037	5.818ProVal: 5.818 ± 0.058	0.886ProTrp: 0.886 ± 0.025	1.166ProTyr: 1.166 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.798GlnAla: 3.798 ± 0.051	0.178GlnCys: 0.178 ± 0.009	1.256GlnAsp: 1.256 ± 0.021	1.33GlnGlu: 1.33 ± 0.021	0.761GlnPhe: 0.761 ± 0.019	2.128GlnGly: 2.128 ± 0.034	0.658GlnHis: 0.658 ± 0.019	1.014GlnIle: 1.014 ± 0.024	0.498GlnLys: 0.498 ± 0.016	3.413GlnLeu: 3.413 ± 0.047	0.417GlnMet: 0.417 ± 0.014	0.51GlnAsn: 0.51 ± 0.018	1.891GlnPro: 1.891 ± 0.038	1.312GlnGln: 1.312 ± 0.031	2.664GlnArg: 2.664 ± 0.035	1.227GlnSer: 1.227 ± 0.022	1.316GlnThr: 1.316 ± 0.028	2.61GlnVal: 2.61 ± 0.032	0.477GlnTrp: 0.477 ± 0.015	0.607GlnTyr: 0.607 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.668ArgAla: 9.668 ± 0.085	0.566ArgCys: 0.566 ± 0.017	4.178ArgAsp: 4.178 ± 0.049	5.299ArgGlu: 5.299 ± 0.056	2.618ArgPhe: 2.618 ± 0.036	5.879ArgGly: 5.879 ± 0.056	1.981ArgHis: 1.981 ± 0.029	3.132ArgIle: 3.132 ± 0.036	1.839ArgLys: 1.839 ± 0.032	8.882ArgLeu: 8.882 ± 0.072	1.799ArgMet: 1.799 ± 0.027	1.473ArgAsn: 1.473 ± 0.027	4.741ArgPro: 4.741 ± 0.056	2.463ArgGln: 2.463 ± 0.038	8.028ArgArg: 8.028 ± 0.085	3.798ArgSer: 3.798 ± 0.042	4.775ArgThr: 4.775 ± 0.047	6.421ArgVal: 6.421 ± 0.057	1.466ArgTrp: 1.466 ± 0.027	2.017ArgTyr: 2.017 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.518SerAla: 6.518 ± 0.056	0.394SerCys: 0.394 ± 0.014	2.458SerAsp: 2.458 ± 0.033	2.405SerGlu: 2.405 ± 0.037	1.594SerPhe: 1.594 ± 0.025	5.731SerGly: 5.731 ± 0.051	0.92SerHis: 0.92 ± 0.022	1.585SerIle: 1.585 ± 0.027	0.939SerLys: 0.939 ± 0.024	4.781SerLeu: 4.781 ± 0.048	1.091SerMet: 1.091 ± 0.019	0.864SerAsn: 0.864 ± 0.019	3.096SerPro: 3.096 ± 0.038	1.213SerGln: 1.213 ± 0.022	3.727SerArg: 3.727 ± 0.046	2.831SerSer: 2.831 ± 0.048	3.172SerThr: 3.172 ± 0.037	4.35SerVal: 4.35 ± 0.047	0.947SerTrp: 0.947 ± 0.022	1.18SerTyr: 1.18 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	7.978ThrAla: 7.978 ± 0.068	0.41ThrCys: 0.41 ± 0.014	3.178ThrAsp: 3.178 ± 0.039	3.313ThrGlu: 3.313 ± 0.037	1.654ThrPhe: 1.654 ± 0.029	6.427ThrGly: 6.427 ± 0.063	1.102ThrHis: 1.102 ± 0.024	1.966ThrIle: 1.966 ± 0.032	1.223ThrLys: 1.223 ± 0.023	5.528ThrLeu: 5.528 ± 0.056	0.881ThrMet: 0.881 ± 0.018	1.045ThrAsn: 1.045 ± 0.022	3.813ThrPro: 3.813 ± 0.048	1.321ThrGln: 1.321 ± 0.028	3.851ThrArg: 3.851 ± 0.042	3.032ThrSer: 3.032 ± 0.037	3.81ThrThr: 3.81 ± 0.05	5.827ThrVal: 5.827 ± 0.059	0.878ThrTrp: 0.878 ± 0.021	1.233ThrTyr: 1.233 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	11.712ValAla: 11.712 ± 0.091	0.747ValCys: 0.747 ± 0.018	5.344ValAsp: 5.344 ± 0.046	5.154ValGlu: 5.154 ± 0.047	2.771ValPhe: 2.771 ± 0.034	6.808ValGly: 6.808 ± 0.059	2.107ValHis: 2.107 ± 0.035	2.831ValIle: 2.831 ± 0.038	1.563ValLys: 1.563 ± 0.028	11.191ValLeu: 11.191 ± 0.092	1.14ValMet: 1.14 ± 0.02	1.801ValAsn: 1.801 ± 0.029	5.622ValPro: 5.622 ± 0.047	2.203ValGln: 2.203 ± 0.03	7.516ValArg: 7.516 ± 0.06	4.723ValSer: 4.723 ± 0.048	5.653ValThr: 5.653 ± 0.049	9.527ValVal: 9.527 ± 0.086	1.093ValTrp: 1.093 ± 0.024	1.532ValTyr: 1.532 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.619TrpAla: 1.619 ± 0.032	0.148TrpCys: 0.148 ± 0.008	0.726TrpAsp: 0.726 ± 0.018	0.655TrpGlu: 0.655 ± 0.018	0.543TrpPhe: 0.543 ± 0.019	1.045TrpGly: 1.045 ± 0.021	0.423TrpHis: 0.423 ± 0.013	0.527TrpIle: 0.527 ± 0.014	0.257TrpLys: 0.257 ± 0.012	2.017TrpLeu: 2.017 ± 0.031	0.245TrpMet: 0.245 ± 0.011	0.349TrpAsn: 0.349 ± 0.014	0.86TrpPro: 0.86 ± 0.021	0.663TrpGln: 0.663 ± 0.018	1.419TrpArg: 1.419 ± 0.028	0.865TrpSer: 0.865 ± 0.023	0.956TrpThr: 0.956 ± 0.023	1.054TrpVal: 1.054 ± 0.024	0.342TrpTrp: 0.342 ± 0.013	0.336TrpTyr: 0.336 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.473TyrAla: 2.473 ± 0.038	0.178TyrCys: 0.178 ± 0.009	1.332TyrAsp: 1.332 ± 0.032	1.107TyrGlu: 1.107 ± 0.022	0.689TyrPhe: 0.689 ± 0.016	2.018TyrGly: 2.018 ± 0.031	0.474TyrHis: 0.474 ± 0.015	0.422TyrIle: 0.422 ± 0.015	0.279TyrLys: 0.279 ± 0.012	2.333TyrLeu: 2.333 ± 0.032	0.18TyrMet: 0.18 ± 0.01	0.416TyrAsn: 0.416 ± 0.015	1.169TyrPro: 1.169 ± 0.026	0.642TyrGln: 0.642 ± 0.018	1.923TyrArg: 1.923 ± 0.033	1.065TyrSer: 1.065 ± 0.022	1.15TyrThr: 1.15 ± 0.026	1.694TyrVal: 1.694 ± 0.026	0.339TyrTrp: 0.339 ± 0.013	0.501TyrTyr: 0.501 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7365 proteins (2303454 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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