Yellowstone lake phycodnavirus 3
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0YNP1|A0A0P0YNP1_9PHYC RING-type domain-containing protein OS=Yellowstone lake phycodnavirus 3 OX=1586715 PE=4 SV=1
MM1 pKa = 7.43 SPNAYY6 pKa = 9.05 VHH8 pKa = 6.67 WDD10 pKa = 3.36 DD11 pKa = 4.75 EE12 pKa = 4.31 KK13 pKa = 11.31 RR14 pKa = 11.84 LLFGFQGEE22 pKa = 4.58 AEE24 pKa = 4.17 HH25 pKa = 6.41 EE26 pKa = 4.34 KK27 pKa = 10.72 PVFTWTYY34 pKa = 8.67 STFEE38 pKa = 4.09 EE39 pKa = 4.9 YY40 pKa = 10.95 LNEE43 pKa = 4.17 ACTPSMYY50 pKa = 10.81 QFMANYY56 pKa = 9.27 IIEE59 pKa = 4.93 EE60 pKa = 3.92 ISKK63 pKa = 11.06 DD64 pKa = 3.57 EE65 pKa = 4.34 LYY67 pKa = 10.68 EE68 pKa = 3.95 LQGGDD73 pKa = 3.75 YY74 pKa = 10.93 CPGRR78 pKa = 11.84 LEE80 pKa = 4.15 EE81 pKa = 4.36 EE82 pKa = 4.13 AVDD85 pKa = 4.92 AYY87 pKa = 11.16 FDD89 pKa = 3.96 LPINEE94 pKa = 4.75 RR95 pKa = 11.84 IEE97 pKa = 4.03 LHH99 pKa = 5.51 NQEE102 pKa = 5.13 LLNLYY107 pKa = 10.29 AEE109 pKa = 4.79 RR110 pKa = 11.84 DD111 pKa = 3.41 RR112 pKa = 11.84 ATGKK116 pKa = 9.72 EE117 pKa = 3.68 AAFIDD122 pKa = 3.93 ACLDD126 pKa = 3.39 DD127 pKa = 4.99 HH128 pKa = 7.53 CPFDD132 pKa = 4.3 VDD134 pKa = 3.74 SPIYY138 pKa = 10.65 VEE140 pKa = 4.09 YY141 pKa = 10.63 CQWCRR146 pKa = 11.84 KK147 pKa = 7.24 QTEE150 pKa = 3.84 KK151 pKa = 7.96 WTKK154 pKa = 9.63 IVARR158 pKa = 11.84 LADD161 pKa = 4.87 EE162 pKa = 3.95 IHH164 pKa = 6.87 EE165 pKa = 4.37 EE166 pKa = 4.25 EE167 pKa = 4.79 DD168 pKa = 3.19 WHH170 pKa = 8.37 SGAEE174 pKa = 4.0 AGASDD179 pKa = 5.26 LEE181 pKa = 4.45 TDD183 pKa = 3.38 EE184 pKa = 6.15 HH185 pKa = 6.75 YY186 pKa = 11.41 DD187 pKa = 3.57 PMNEE191 pKa = 3.77
Molecular weight: 22.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.942
IPC2_protein 4.177
IPC_protein 4.126
Toseland 3.961
ProMoST 4.215
Dawson 4.075
Bjellqvist 4.253
Wikipedia 3.948
Rodwell 3.961
Grimsley 3.872
Solomon 4.075
Lehninger 4.024
Nozaki 4.19
DTASelect 4.329
Thurlkill 3.973
EMBOSS 3.961
Sillero 4.24
Patrickios 0.858
IPC_peptide 4.075
IPC2_peptide 4.228
IPC2.peptide.svr19 4.14
Protein with the highest isoelectric point:
>tr|A0A0N7KVY5|A0A0N7KVY5_9PHYC Uncharacterized protein OS=Yellowstone lake phycodnavirus 3 OX=1586715 PE=4 SV=1
MM1 pKa = 6.04 TTTRR5 pKa = 11.84 IRR7 pKa = 11.84 RR8 pKa = 11.84 TTSPARR14 pKa = 11.84 TAKK17 pKa = 8.95 KK18 pKa = 7.55 TASPARR24 pKa = 11.84 TASPANKK31 pKa = 9.76 RR32 pKa = 11.84 PRR34 pKa = 11.84 NAPNPWAPKK43 pKa = 7.98 YY44 pKa = 8.69 TWAPWGTSGVVHH56 pKa = 6.75 DD57 pKa = 4.59 NCYY60 pKa = 11.01 DD61 pKa = 3.52 YY62 pKa = 11.94 AFGSFSNNRR71 pKa = 11.84 TEE73 pKa = 3.96 KK74 pKa = 10.39 SVPGVRR80 pKa = 11.84 AKK82 pKa = 10.95 LNSNGMNFRR91 pKa = 11.84 TCDD94 pKa = 3.77 GIAKK98 pKa = 9.83 RR99 pKa = 11.84 VLADD103 pKa = 3.43 NPGNVYY109 pKa = 10.57 KK110 pKa = 10.42 MKK112 pKa = 10.86 SGDD115 pKa = 3.93 EE116 pKa = 3.96 KK117 pKa = 10.95 PKK119 pKa = 10.16 AGFYY123 pKa = 10.9 KK124 pKa = 10.35 VMCFVAPTNDD134 pKa = 3.4 FGNSTGDD141 pKa = 3.19 FHH143 pKa = 6.94 WYY145 pKa = 10.31 KK146 pKa = 10.28 EE147 pKa = 3.99 ISAIRR152 pKa = 11.84 YY153 pKa = 7.43 KK154 pKa = 10.2 IRR156 pKa = 11.84 RR157 pKa = 11.84 ADD159 pKa = 3.79 TIMGMAKK166 pKa = 10.07 FFHH169 pKa = 5.89 VTPKK173 pKa = 9.93 TIRR176 pKa = 11.84 DD177 pKa = 4.1 AIAKK181 pKa = 9.37 GRR183 pKa = 11.84 ASANANNGRR192 pKa = 11.84 VANKK196 pKa = 8.59 NTDD199 pKa = 3.32 LHH201 pKa = 6.51 VLNSMSAASRR211 pKa = 11.84 NKK213 pKa = 8.76 MPVPVGRR220 pKa = 11.84 VIDD223 pKa = 4.05 FPVKK227 pKa = 10.15 LWSHH231 pKa = 4.92 KK232 pKa = 9.14 TGWAGGPLIVDD243 pKa = 4.09 ASGKK247 pKa = 9.06 TITDD251 pKa = 3.08 PRR253 pKa = 11.84 RR254 pKa = 11.84 CDD256 pKa = 3.12 RR257 pKa = 11.84 KK258 pKa = 8.82 FTPGFHH264 pKa = 4.95 YY265 pKa = 9.79 TKK267 pKa = 10.11 FCSAYY272 pKa = 8.73 GVRR275 pKa = 11.84 RR276 pKa = 11.84 GFAKK280 pKa = 10.07 TGNNANRR287 pKa = 11.84 NN288 pKa = 3.73
Molecular weight: 31.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.823
IPC_protein 10.394
Toseland 10.789
ProMoST 10.379
Dawson 10.877
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.272
Grimsley 10.906
Solomon 10.95
Lehninger 10.921
Nozaki 10.76
DTASelect 10.526
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.804
Patrickios 10.979
IPC_peptide 10.965
IPC2_peptide 9.414
IPC2.peptide.svr19 8.553
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
236
0
236
53188
53
1432
225.4
24.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.634 ± 0.313
1.542 ± 0.122
4.715 ± 0.26
5.076 ± 0.294
4.172 ± 0.117
7.165 ± 0.227
1.688 ± 0.122
5.334 ± 0.11
6.067 ± 0.334
8.062 ± 0.166
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.76 ± 0.119
5.443 ± 0.32
5.356 ± 0.14
3.495 ± 0.112
5.721 ± 0.35
6.515 ± 0.287
6.618 ± 0.448
7.069 ± 0.204
1.324 ± 0.068
3.243 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here