Opium poppy mosaic virus
Average proteome isoelectric point is 7.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G0KUS6|G0KUS6_9TOMB Putative phloem RNA movement protein OS=Opium poppy mosaic virus OX=473784 PE=4 SV=2
MM1 pKa = 7.88 DD2 pKa = 4.05 FVDD5 pKa = 6.31 RR6 pKa = 11.84 ITRR9 pKa = 11.84 WLSGDD14 pKa = 4.47 FKK16 pKa = 10.69 PSAYY20 pKa = 9.23 IKK22 pKa = 10.56 SRR24 pKa = 11.84 NEE26 pKa = 3.56 CVVAYY31 pKa = 10.15 GEE33 pKa = 4.13 EE34 pKa = 4.19 WATLVTQSRR43 pKa = 11.84 MQRR46 pKa = 11.84 EE47 pKa = 4.24 ASRR50 pKa = 11.84 QYY52 pKa = 11.54 ADD54 pKa = 3.56 WYY56 pKa = 10.84 NSASPTTCTMPPPTDD71 pKa = 3.32 AEE73 pKa = 4.6 LIEE76 pKa = 4.12 EE77 pKa = 4.16 ARR79 pKa = 11.84 VGKK82 pKa = 10.39 LKK84 pKa = 10.95 AGLDD88 pKa = 3.65 QPKK91 pKa = 9.49 KK92 pKa = 7.79 QQEE95 pKa = 3.95 AASINLGGIKK105 pKa = 9.19 TPPPMSEE112 pKa = 4.06 EE113 pKa = 3.9 QSTLLDD119 pKa = 3.84 LEE121 pKa = 4.79 ALPSYY126 pKa = 10.33 DD127 pKa = 3.78 QIVAVGKK134 pKa = 9.96 LRR136 pKa = 11.84 RR137 pKa = 11.84 LQLQSACPQYY147 pKa = 10.68 TDD149 pKa = 3.76 EE150 pKa = 5.38 EE151 pKa = 4.35 RR152 pKa = 11.84 ASWAIIPYY160 pKa = 8.74 TGGPRR165 pKa = 11.84 VIRR168 pKa = 11.84 EE169 pKa = 4.14 DD170 pKa = 3.49 EE171 pKa = 4.22 VLPRR175 pKa = 11.84 APSVVLLNDD184 pKa = 3.83 APRR187 pKa = 11.84 SMSKK191 pKa = 9.62 IEE193 pKa = 4.19 EE194 pKa = 4.15 LVKK197 pKa = 10.99 ACFATIDD204 pKa = 4.36 KK205 pKa = 9.0 VAKK208 pKa = 8.0 WRR210 pKa = 11.84 KK211 pKa = 8.43 VDD213 pKa = 4.02 YY214 pKa = 10.8 IDD216 pKa = 4.7 FSSKK220 pKa = 10.46 AAQTPPSKK228 pKa = 11.03 YY229 pKa = 9.9 KK230 pKa = 10.22 LQEE233 pKa = 4.17 PGEE236 pKa = 4.33 VMKK239 pKa = 10.91 GMNAQSVAMEE249 pKa = 3.93 LRR251 pKa = 11.84 CRR253 pKa = 11.84 YY254 pKa = 8.86 GVQPCTAANLQLGNRR269 pKa = 11.84 VARR272 pKa = 11.84 EE273 pKa = 3.55 ILDD276 pKa = 3.83 RR277 pKa = 11.84 QCQATRR283 pKa = 11.84 EE284 pKa = 3.87 QVYY287 pKa = 10.04 YY288 pKa = 10.02 YY289 pKa = 11.0 GHH291 pKa = 7.86 LATTMWFQPTLVDD304 pKa = 3.84 LALRR308 pKa = 11.84 AGAKK312 pKa = 10.32 DD313 pKa = 3.36 FF314 pKa = 4.45
Molecular weight: 35.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.513
IPC2_protein 6.707
IPC_protein 6.81
Toseland 6.364
ProMoST 7.146
Dawson 7.454
Bjellqvist 7.483
Wikipedia 7.351
Rodwell 7.439
Grimsley 6.338
Solomon 7.512
Lehninger 7.541
Nozaki 7.79
DTASelect 7.556
Thurlkill 7.6
EMBOSS 7.673
Sillero 7.922
Patrickios 4.24
IPC_peptide 7.512
IPC2_peptide 6.985
IPC2.peptide.svr19 7.111
Protein with the highest isoelectric point:
>tr|G0KUS7|G0KUS7_9TOMB Putative cell-to-cell movement protein OS=Opium poppy mosaic virus OX=473784 PE=4 SV=2
MM1 pKa = 7.73 ASLPNVSSSSKK12 pKa = 10.75 SRR14 pKa = 11.84 MQGRR18 pKa = 11.84 TTQRR22 pKa = 11.84 AIRR25 pKa = 11.84 RR26 pKa = 11.84 GDD28 pKa = 3.32 GQRR31 pKa = 11.84 ARR33 pKa = 11.84 RR34 pKa = 11.84 NRR36 pKa = 11.84 PGHH39 pKa = 5.95 TDD41 pKa = 3.46 PPPSRR46 pKa = 11.84 AEE48 pKa = 3.72 ACRR51 pKa = 11.84 NPAPAPKK58 pKa = 10.42 NPGKK62 pKa = 10.27 NRR64 pKa = 11.84 GSLEE68 pKa = 3.99 EE69 pKa = 4.2 TPHH72 pKa = 5.77 HH73 pKa = 6.51 KK74 pKa = 9.97 KK75 pKa = 9.9 HH76 pKa = 6.57 RR77 pKa = 11.84 GPVVPRR83 pKa = 11.84 EE84 pKa = 4.25 NRR86 pKa = 11.84 GSVHH90 pKa = 4.96 PTRR93 pKa = 11.84 PRR95 pKa = 11.84 RR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 GDD102 pKa = 3.08 HH103 pKa = 5.57 MGSRR107 pKa = 11.84 QHH109 pKa = 6.22 ATTPEE114 pKa = 4.17 QCWGACVLSTEE125 pKa = 4.2 RR126 pKa = 11.84 RR127 pKa = 11.84 AEE129 pKa = 3.82 IDD131 pKa = 3.54 GLLSPLLHH139 pKa = 6.16 TALMQGWGDD148 pKa = 3.84 PEE150 pKa = 4.09 VLVYY154 pKa = 9.8 SIRR157 pKa = 11.84 VLRR160 pKa = 11.84 GEE162 pKa = 3.8 LRR164 pKa = 11.84 QWRR167 pKa = 11.84 KK168 pKa = 8.73 PVQSLHH174 pKa = 6.07 NVATEE179 pKa = 3.85 DD180 pKa = 3.65 RR181 pKa = 11.84 GNSAQLSAPIATEE194 pKa = 4.07 PPNMQAPSKK203 pKa = 8.99 EE204 pKa = 3.9 QPAVDD209 pKa = 4.76 GSPKK213 pKa = 9.86 ICNLGCNIDD222 pKa = 4.12 KK223 pKa = 11.0 VCGHH227 pKa = 7.29 PKK229 pKa = 9.87 PGEE232 pKa = 4.28 GNSGSTTQAGNTWW245 pKa = 3.06
Molecular weight: 26.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.56
IPC_protein 10.657
Toseland 11.052
ProMoST 11.199
Dawson 11.082
Bjellqvist 10.906
Wikipedia 11.403
Rodwell 11.082
Grimsley 11.111
Solomon 11.403
Lehninger 11.33
Nozaki 11.052
DTASelect 10.906
Thurlkill 11.038
EMBOSS 11.491
Sillero 11.038
Patrickios 10.818
IPC_peptide 11.403
IPC2_peptide 10.321
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1681
245
865
420.3
47.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.626 ± 0.457
2.32 ± 0.089
4.819 ± 0.75
6.068 ± 0.333
2.558 ± 0.638
6.246 ± 0.603
2.142 ± 0.483
4.521 ± 0.449
5.473 ± 0.558
8.15 ± 0.593
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.677 ± 0.25
3.629 ± 0.427
7.02 ± 0.905
4.521 ± 0.57
7.436 ± 0.885
6.425 ± 0.455
5.532 ± 0.134
6.901 ± 0.438
1.725 ± 0.219
3.212 ± 0.545
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here