Agrobacterium phage Atu_ph07

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Polybotosvirus; Agrobacterium virus Atuph07

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 714 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0UZN5|A0A2L0UZN5_9CAUD Uncharacterized protein OS=Agrobacterium phage Atu_ph07 OX=2024264 PE=4 SV=1
MM1 pKa = 7.98LDD3 pKa = 3.36TEE5 pKa = 4.55NEE7 pKa = 3.4IGYY10 pKa = 8.5TIDD13 pKa = 2.89ILYY16 pKa = 10.3DD17 pKa = 3.37IFNGEE22 pKa = 4.47SYY24 pKa = 10.66TFFATTMEE32 pKa = 4.52SFSQILPMSLPNPNDD47 pKa = 2.79ISKK50 pKa = 10.31IYY52 pKa = 10.41IGDD55 pKa = 3.93FSYY58 pKa = 11.27FEE60 pKa = 4.75NDD62 pKa = 3.27TGIHH66 pKa = 6.34RR67 pKa = 11.84ADD69 pKa = 3.31TMQEE73 pKa = 3.79IIEE76 pKa = 4.93KK77 pKa = 10.23ISSLTT82 pKa = 3.42

Molecular weight: 9.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0V0X0|A0A2L0V0X0_9CAUD Threonyl-tRNA synthetase OS=Agrobacterium phage Atu_ph07 OX=2024264 PE=4 SV=1
MM1 pKa = 7.25FAFDD5 pKa = 4.19AVFEE9 pKa = 4.35IRR11 pKa = 11.84WANDD15 pKa = 2.52ITEE18 pKa = 4.19TRR20 pKa = 11.84RR21 pKa = 11.84FEE23 pKa = 4.06IMCRR27 pKa = 11.84RR28 pKa = 11.84SALVRR33 pKa = 11.84SAKK36 pKa = 8.79KK37 pKa = 6.61TTGIYY42 pKa = 10.61VSTKK46 pKa = 9.78RR47 pKa = 11.84LFNSGMFRR55 pKa = 11.84KK56 pKa = 7.98TFF58 pKa = 3.35

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

714

0

714

134448

37

4449

188.3

21.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.38 ± 0.109

0.794 ± 0.041

7.038 ± 0.097

6.822 ± 0.138

4.962 ± 0.081

6.17 ± 0.186

1.785 ± 0.054

7.813 ± 0.106

6.803 ± 0.154

7.23 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.542 ± 0.071

6.251 ± 0.086

3.676 ± 0.079

2.51 ± 0.064

4.129 ± 0.077

6.758 ± 0.096

6.6 ± 0.151

6.83 ± 0.082

1.279 ± 0.063

4.629 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski