Murine feces-associated gemycircularvirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; unclassified Gemycircularvirus

Average proteome isoelectric point is 8.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0SZ51|A0A2S0SZ51_9VIRU Cap OS=Murine feces-associated gemycircularvirus 2 OX=2171385 PE=4 SV=1
MM1 pKa = 7.45SNDD4 pKa = 2.79SSTRR8 pKa = 11.84EE9 pKa = 4.04LLSLTVSRR17 pKa = 11.84RR18 pKa = 11.84ILEE21 pKa = 4.03ILDD24 pKa = 3.8AVGSDD29 pKa = 3.51ASITAMEE36 pKa = 4.16ASITTFTLVSTRR48 pKa = 11.84LQLSTRR54 pKa = 11.84RR55 pKa = 11.84KK56 pKa = 9.07RR57 pKa = 11.84SIISEE62 pKa = 3.94LTEE65 pKa = 3.58ILNQYY70 pKa = 8.95EE71 pKa = 3.99EE72 pKa = 4.4HH73 pKa = 5.99QRR75 pKa = 11.84SLLTMQARR83 pKa = 11.84RR84 pKa = 11.84EE85 pKa = 4.12MYY87 pKa = 10.24DD88 pKa = 2.64WTTANPHH95 pKa = 6.03DD96 pKa = 4.57HH97 pKa = 6.87LLNVASMIQINGQISSAAQIKK118 pKa = 9.59NHH120 pKa = 6.19FCTLYY125 pKa = 10.23VRR127 pKa = 11.84RR128 pKa = 11.84LQEE131 pKa = 3.69IGCYY135 pKa = 9.19PIRR138 pKa = 11.84GSSSMPTYY146 pKa = 9.64TIPTRR151 pKa = 11.84SPNINHH157 pKa = 6.72RR158 pKa = 11.84PSLHH162 pKa = 6.58RR163 pKa = 11.84MNATLSSSNGSNRR176 pKa = 11.84HH177 pKa = 4.41TLEE180 pKa = 3.77SHH182 pKa = 5.56EE183 pKa = 4.26MRR185 pKa = 11.84GKK187 pKa = 10.87LSLLAAGPAPGCGNRR202 pKa = 11.84RR203 pKa = 11.84GGCAPTPLRR212 pKa = 11.84TEE214 pKa = 4.16WNWANWRR221 pKa = 11.84RR222 pKa = 11.84VKK224 pKa = 10.84SLILWGGTRR233 pKa = 11.84TGKK236 pKa = 8.13TLYY239 pKa = 10.48ARR241 pKa = 11.84SLGHH245 pKa = 6.0HH246 pKa = 7.26AYY248 pKa = 11.04YY249 pKa = 10.88NLQFNMDD256 pKa = 4.81DD257 pKa = 3.87FSNDD261 pKa = 2.4CKK263 pKa = 11.1YY264 pKa = 11.29AVFDD268 pKa = 5.17DD269 pKa = 3.78IQGGFEE275 pKa = 3.92YY276 pKa = 9.65WHH278 pKa = 6.92SYY280 pKa = 9.54KK281 pKa = 10.77GWLGAQKK288 pKa = 10.48QFVITDD294 pKa = 3.92KK295 pKa = 10.7YY296 pKa = 9.09RR297 pKa = 11.84KK298 pKa = 9.5KK299 pKa = 10.11RR300 pKa = 11.84TIHH303 pKa = 5.65WGKK306 pKa = 9.61PSIMCTNDD314 pKa = 3.51DD315 pKa = 4.26PFSMRR320 pKa = 11.84NVDD323 pKa = 4.88YY324 pKa = 10.98DD325 pKa = 3.24WLMNNCYY332 pKa = 9.79IINVVDD338 pKa = 4.89PICIVSSLPSSSDD351 pKa = 3.34NQSEE355 pKa = 4.35QII357 pKa = 3.76

Molecular weight: 40.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0SZ51|A0A2S0SZ51_9VIRU Cap OS=Murine feces-associated gemycircularvirus 2 OX=2171385 PE=4 SV=1
MM1 pKa = 7.69ALRR4 pKa = 11.84SRR6 pKa = 11.84FYY8 pKa = 10.18RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84MGRR15 pKa = 11.84SYY17 pKa = 10.62RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84PVRR23 pKa = 11.84RR24 pKa = 11.84YY25 pKa = 8.9SRR27 pKa = 11.84RR28 pKa = 11.84GRR30 pKa = 11.84SGYY33 pKa = 10.52GRR35 pKa = 11.84GGYY38 pKa = 7.78NRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84YY43 pKa = 9.23RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84SWISPYY54 pKa = 10.45DD55 pKa = 3.53KK56 pKa = 10.94KK57 pKa = 11.1RR58 pKa = 11.84DD59 pKa = 3.9VVKK62 pKa = 10.51GAHH65 pKa = 6.16DD66 pKa = 3.68TNDD69 pKa = 3.08NSFAPIGAPNTFFGFCPTYY88 pKa = 10.07MPNAVAGDD96 pKa = 3.98TGHH99 pKa = 6.19SHH101 pKa = 5.51QKK103 pKa = 9.93VRR105 pKa = 11.84MSKK108 pKa = 9.51NVGFTGYY115 pKa = 7.79QEE117 pKa = 4.12RR118 pKa = 11.84VHH120 pKa = 6.38IATAEE125 pKa = 3.98SLIWRR130 pKa = 11.84RR131 pKa = 11.84IVIWSYY137 pKa = 11.43NRR139 pKa = 11.84LNNTAGPTKK148 pKa = 9.82TDD150 pKa = 3.12SDD152 pKa = 4.11GVSYY156 pKa = 7.39TTRR159 pKa = 11.84QITPLQLTEE168 pKa = 3.85EE169 pKa = 4.21VRR171 pKa = 11.84RR172 pKa = 11.84FLFRR176 pKa = 11.84GTQGVDD182 pKa = 3.16YY183 pKa = 10.72TEE185 pKa = 4.36NTLHH189 pKa = 6.48EE190 pKa = 4.74APLNADD196 pKa = 3.86HH197 pKa = 7.03FVVAYY202 pKa = 10.34DD203 pKa = 3.6RR204 pKa = 11.84TRR206 pKa = 11.84VLQPRR211 pKa = 11.84RR212 pKa = 11.84PTGDD216 pKa = 2.99EE217 pKa = 3.82YY218 pKa = 12.03GHH220 pKa = 6.44IFNMKK225 pKa = 8.4FWNPGGRR232 pKa = 11.84IIYY235 pKa = 10.4SDD237 pKa = 4.94DD238 pKa = 3.49EE239 pKa = 6.21DD240 pKa = 5.15GLTKK244 pKa = 10.78GPLVNGWSSLGRR256 pKa = 11.84ASKK259 pKa = 11.08GNMYY263 pKa = 10.41IFDD266 pKa = 4.53LFSTGFSLTSNTPSIARR283 pKa = 11.84FAPTGCRR290 pKa = 11.84YY291 pKa = 5.61WTEE294 pKa = 4.05GG295 pKa = 3.23

Molecular weight: 34.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

652

295

357

326.0

37.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.215 ± 0.078

1.534 ± 0.514

4.908 ± 0.106

3.528 ± 0.286

3.528 ± 0.935

7.515 ± 1.39

2.914 ± 0.325

5.675 ± 0.965

3.221 ± 0.102

6.902 ± 1.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.761 ± 0.436

5.982 ± 0.335

4.448 ± 0.382

3.067 ± 0.621

10.736 ± 2.102

9.202 ± 1.251

7.669 ± 0.28

4.141 ± 0.567

2.301 ± 0.16

4.755 ± 0.605
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski