Amino acid dipepetide frequency for Lactobacillus phage Lpa804

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.536AlaAla: 2.536 ± 0.458
0.211AlaCys: 0.211 ± 0.082
3.769AlaAsp: 3.769 ± 0.393
3.346AlaGlu: 3.346 ± 0.35
1.972AlaPhe: 1.972 ± 0.262
4.508AlaGly: 4.508 ± 0.72
0.669AlaHis: 0.669 ± 0.125
3.804AlaIle: 3.804 ± 0.34
6.481AlaLys: 6.481 ± 0.669
5.248AlaLeu: 5.248 ± 0.416
1.515AlaMet: 1.515 ± 0.231
3.91AlaAsn: 3.91 ± 0.518
2.078AlaPro: 2.078 ± 0.339
2.254AlaGln: 2.254 ± 0.29
2.289AlaArg: 2.289 ± 0.252
4.614AlaSer: 4.614 ± 0.423
4.614AlaThr: 4.614 ± 0.409
3.874AlaVal: 3.874 ± 0.385
0.599AlaTrp: 0.599 ± 0.14
2.712AlaTyr: 2.712 ± 0.333
0.0AlaXaa: 0.0 ± 0.0
Cys
0.211CysAla: 0.211 ± 0.084
0.0CysCys: 0.0 ± 0.0
0.458CysAsp: 0.458 ± 0.128
0.282CysGlu: 0.282 ± 0.098
0.282CysPhe: 0.282 ± 0.095
0.493CysGly: 0.493 ± 0.194
0.211CysHis: 0.211 ± 0.088
0.458CysIle: 0.458 ± 0.116
0.599CysLys: 0.599 ± 0.129
0.599CysLeu: 0.599 ± 0.146
0.07CysMet: 0.07 ± 0.048
0.247CysAsn: 0.247 ± 0.111
0.247CysPro: 0.247 ± 0.112
0.176CysGln: 0.176 ± 0.072
0.141CysArg: 0.141 ± 0.07
0.528CysSer: 0.528 ± 0.167
0.317CysThr: 0.317 ± 0.118
0.352CysVal: 0.352 ± 0.089
0.035CysTrp: 0.035 ± 0.033
0.282CysTyr: 0.282 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
4.332AspAla: 4.332 ± 0.395
0.74AspCys: 0.74 ± 0.197
5.635AspAsp: 5.635 ± 0.6
4.191AspGlu: 4.191 ± 0.556
2.642AspPhe: 2.642 ± 0.324
4.403AspGly: 4.403 ± 0.469
0.564AspHis: 0.564 ± 0.134
5.354AspIle: 5.354 ± 0.398
6.058AspLys: 6.058 ± 0.585
6.445AspLeu: 6.445 ± 0.587
2.43AspMet: 2.43 ± 0.258
5.6AspAsn: 5.6 ± 0.401
2.325AspPro: 2.325 ± 0.29
1.515AspGln: 1.515 ± 0.283
2.254AspArg: 2.254 ± 0.24
5.178AspSer: 5.178 ± 0.348
4.649AspThr: 4.649 ± 0.413
4.086AspVal: 4.086 ± 0.434
0.881AspTrp: 0.881 ± 0.16
3.663AspTyr: 3.663 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
4.684GluAla: 4.684 ± 0.624
0.282GluCys: 0.282 ± 0.109
4.473GluAsp: 4.473 ± 0.512
4.367GluGlu: 4.367 ± 0.566
1.902GluPhe: 1.902 ± 0.248
2.782GluGly: 2.782 ± 0.346
1.479GluHis: 1.479 ± 0.218
3.381GluIle: 3.381 ± 0.334
3.839GluLys: 3.839 ± 0.368
6.481GluLeu: 6.481 ± 0.573
1.409GluMet: 1.409 ± 0.173
2.959GluAsn: 2.959 ± 0.294
1.62GluPro: 1.62 ± 0.306
2.219GluGln: 2.219 ± 0.257
2.606GluArg: 2.606 ± 0.24
4.015GluSer: 4.015 ± 0.437
3.593GluThr: 3.593 ± 0.387
3.17GluVal: 3.17 ± 0.385
0.528GluTrp: 0.528 ± 0.152
3.029GluTyr: 3.029 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
1.479PheAla: 1.479 ± 0.243
0.282PheCys: 0.282 ± 0.108
2.606PheAsp: 2.606 ± 0.318
2.113PheGlu: 2.113 ± 0.305
0.81PhePhe: 0.81 ± 0.22
2.008PheGly: 2.008 ± 0.27
0.669PheHis: 0.669 ± 0.156
2.148PheIle: 2.148 ± 0.297
3.416PheLys: 3.416 ± 0.314
2.219PheLeu: 2.219 ± 0.328
0.881PheMet: 0.881 ± 0.159
3.663PheAsn: 3.663 ± 0.392
1.162PhePro: 1.162 ± 0.236
1.057PheGln: 1.057 ± 0.204
1.55PheArg: 1.55 ± 0.188
3.205PheSer: 3.205 ± 0.312
2.148PheThr: 2.148 ± 0.301
2.536PheVal: 2.536 ± 0.346
0.352PheTrp: 0.352 ± 0.106
1.55PheTyr: 1.55 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
3.416GlyAla: 3.416 ± 0.556
0.282GlyCys: 0.282 ± 0.1
4.262GlyAsp: 4.262 ± 0.387
3.874GlyGlu: 3.874 ± 0.376
2.747GlyPhe: 2.747 ± 0.344
4.05GlyGly: 4.05 ± 0.494
0.951GlyHis: 0.951 ± 0.16
4.684GlyIle: 4.684 ± 0.47
5.001GlyLys: 5.001 ± 0.506
4.966GlyLeu: 4.966 ± 0.457
1.55GlyMet: 1.55 ± 0.255
4.086GlyAsn: 4.086 ± 0.353
0.528GlyPro: 0.528 ± 0.139
1.867GlyGln: 1.867 ± 0.251
1.796GlyArg: 1.796 ± 0.279
4.72GlySer: 4.72 ± 0.705
4.227GlyThr: 4.227 ± 0.61
4.931GlyVal: 4.931 ± 0.485
0.81GlyTrp: 0.81 ± 0.163
3.628GlyTyr: 3.628 ± 0.312
0.0GlyXaa: 0.0 ± 0.0
His
0.881HisAla: 0.881 ± 0.167
0.282HisCys: 0.282 ± 0.132
1.585HisAsp: 1.585 ± 0.24
0.881HisGlu: 0.881 ± 0.157
0.704HisPhe: 0.704 ± 0.153
1.057HisGly: 1.057 ± 0.201
0.247HisHis: 0.247 ± 0.112
0.986HisIle: 0.986 ± 0.224
1.585HisLys: 1.585 ± 0.258
0.951HisLeu: 0.951 ± 0.187
0.352HisMet: 0.352 ± 0.106
1.268HisAsn: 1.268 ± 0.196
0.599HisPro: 0.599 ± 0.143
0.423HisGln: 0.423 ± 0.097
0.528HisArg: 0.528 ± 0.158
0.916HisSer: 0.916 ± 0.163
0.704HisThr: 0.704 ± 0.177
0.986HisVal: 0.986 ± 0.195
0.211HisTrp: 0.211 ± 0.086
0.845HisTyr: 0.845 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
3.628IleAla: 3.628 ± 0.355
0.282IleCys: 0.282 ± 0.106
5.459IleAsp: 5.459 ± 0.428
3.98IleGlu: 3.98 ± 0.445
1.233IlePhe: 1.233 ± 0.193
3.733IleGly: 3.733 ± 0.366
0.775IleHis: 0.775 ± 0.182
3.663IleIle: 3.663 ± 0.387
5.847IleLys: 5.847 ± 0.399
3.24IleLeu: 3.24 ± 0.359
1.62IleMet: 1.62 ± 0.251
4.72IleAsn: 4.72 ± 0.526
2.325IlePro: 2.325 ± 0.333
1.937IleGln: 1.937 ± 0.276
2.536IleArg: 2.536 ± 0.308
5.847IleSer: 5.847 ± 0.432
4.579IleThr: 4.579 ± 0.39
4.332IleVal: 4.332 ± 0.413
0.387IleTrp: 0.387 ± 0.128
2.818IleTyr: 2.818 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
5.741LysAla: 5.741 ± 0.576
0.528LysCys: 0.528 ± 0.11
5.354LysAsp: 5.354 ± 0.523
5.318LysGlu: 5.318 ± 0.645
3.24LysPhe: 3.24 ± 0.361
4.896LysGly: 4.896 ± 0.553
1.409LysHis: 1.409 ± 0.243
3.874LysIle: 3.874 ± 0.428
5.283LysLys: 5.283 ± 0.599
7.256LysLeu: 7.256 ± 0.625
2.395LysMet: 2.395 ± 0.235
4.332LysAsn: 4.332 ± 0.444
2.677LysPro: 2.677 ± 0.349
3.698LysGln: 3.698 ± 0.415
3.416LysArg: 3.416 ± 0.394
5.741LysSer: 5.741 ± 0.757
4.015LysThr: 4.015 ± 0.317
5.424LysVal: 5.424 ± 0.381
0.986LysTrp: 0.986 ± 0.242
3.698LysTyr: 3.698 ± 0.43
0.0LysXaa: 0.0 ± 0.0
Leu
5.107LeuAla: 5.107 ± 0.412
0.387LeuCys: 0.387 ± 0.123
6.692LeuAsp: 6.692 ± 0.596
5.178LeuGlu: 5.178 ± 0.382
3.346LeuPhe: 3.346 ± 0.376
4.896LeuGly: 4.896 ± 0.414
1.479LeuHis: 1.479 ± 0.196
4.79LeuIle: 4.79 ± 0.47
5.988LeuLys: 5.988 ± 0.53
6.023LeuLeu: 6.023 ± 0.603
1.937LeuMet: 1.937 ± 0.257
5.882LeuAsn: 5.882 ± 0.441
2.853LeuPro: 2.853 ± 0.394
3.064LeuGln: 3.064 ± 0.33
2.782LeuArg: 2.782 ± 0.321
7.432LeuSer: 7.432 ± 0.496
5.882LeuThr: 5.882 ± 0.465
5.001LeuVal: 5.001 ± 0.312
0.775LeuTrp: 0.775 ± 0.172
3.452LeuTyr: 3.452 ± 0.315
0.0LeuXaa: 0.0 ± 0.0
Met
2.148MetAla: 2.148 ± 0.299
0.106MetCys: 0.106 ± 0.054
1.409MetAsp: 1.409 ± 0.252
1.515MetGlu: 1.515 ± 0.206
0.916MetPhe: 0.916 ± 0.178
1.021MetGly: 1.021 ± 0.17
0.352MetHis: 0.352 ± 0.112
1.479MetIle: 1.479 ± 0.242
2.043MetLys: 2.043 ± 0.232
2.219MetLeu: 2.219 ± 0.261
0.352MetMet: 0.352 ± 0.169
1.62MetAsn: 1.62 ± 0.241
0.599MetPro: 0.599 ± 0.149
1.127MetGln: 1.127 ± 0.212
0.775MetArg: 0.775 ± 0.184
1.761MetSer: 1.761 ± 0.273
1.585MetThr: 1.585 ± 0.243
1.233MetVal: 1.233 ± 0.244
0.141MetTrp: 0.141 ± 0.069
0.881MetTyr: 0.881 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.839AsnAla: 3.839 ± 0.373
0.282AsnCys: 0.282 ± 0.102
4.508AsnAsp: 4.508 ± 0.452
3.487AsnGlu: 3.487 ± 0.356
2.536AsnPhe: 2.536 ± 0.323
5.142AsnGly: 5.142 ± 0.521
1.338AsnHis: 1.338 ± 0.206
4.086AsnIle: 4.086 ± 0.409
5.882AsnLys: 5.882 ± 0.479
5.424AsnLeu: 5.424 ± 0.474
1.655AsnMet: 1.655 ± 0.257
4.473AsnAsn: 4.473 ± 0.456
2.853AsnPro: 2.853 ± 0.356
2.923AsnGln: 2.923 ± 0.307
2.782AsnArg: 2.782 ± 0.301
5.213AsnSer: 5.213 ± 0.543
3.98AsnThr: 3.98 ± 0.442
3.874AsnVal: 3.874 ± 0.393
0.564AsnTrp: 0.564 ± 0.139
3.839AsnTyr: 3.839 ± 0.436
0.0AsnXaa: 0.0 ± 0.0
Pro
1.515ProAla: 1.515 ± 0.266
0.247ProCys: 0.247 ± 0.1
2.043ProAsp: 2.043 ± 0.333
2.782ProGlu: 2.782 ± 0.421
1.127ProPhe: 1.127 ± 0.178
0.881ProGly: 0.881 ± 0.163
0.528ProHis: 0.528 ± 0.134
1.937ProIle: 1.937 ± 0.277
2.818ProLys: 2.818 ± 0.351
3.029ProLeu: 3.029 ± 0.328
0.423ProMet: 0.423 ± 0.098
2.289ProAsn: 2.289 ± 0.314
0.423ProPro: 0.423 ± 0.121
0.916ProGln: 0.916 ± 0.246
1.162ProArg: 1.162 ± 0.193
2.395ProSer: 2.395 ± 0.268
2.008ProThr: 2.008 ± 0.287
2.254ProVal: 2.254 ± 0.321
0.176ProTrp: 0.176 ± 0.069
1.761ProTyr: 1.761 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
3.24GlnAla: 3.24 ± 0.481
0.211GlnCys: 0.211 ± 0.094
2.289GlnAsp: 2.289 ± 0.293
2.043GlnGlu: 2.043 ± 0.272
1.233GlnPhe: 1.233 ± 0.22
2.325GlnGly: 2.325 ± 0.327
0.458GlnHis: 0.458 ± 0.112
2.043GlnIle: 2.043 ± 0.286
1.726GlnLys: 1.726 ± 0.226
3.522GlnLeu: 3.522 ± 0.288
0.704GlnMet: 0.704 ± 0.19
1.832GlnAsn: 1.832 ± 0.237
1.233GlnPro: 1.233 ± 0.328
1.761GlnGln: 1.761 ± 0.305
1.444GlnArg: 1.444 ± 0.201
3.064GlnSer: 3.064 ± 0.368
1.796GlnThr: 1.796 ± 0.304
2.642GlnVal: 2.642 ± 0.323
0.282GlnTrp: 0.282 ± 0.111
1.338GlnTyr: 1.338 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
2.113ArgAla: 2.113 ± 0.207
0.247ArgCys: 0.247 ± 0.087
2.465ArgAsp: 2.465 ± 0.281
1.62ArgGlu: 1.62 ± 0.308
1.655ArgPhe: 1.655 ± 0.235
2.818ArgGly: 2.818 ± 0.406
0.352ArgHis: 0.352 ± 0.148
2.36ArgIle: 2.36 ± 0.312
2.536ArgLys: 2.536 ± 0.324
3.522ArgLeu: 3.522 ± 0.334
1.127ArgMet: 1.127 ± 0.193
1.972ArgAsn: 1.972 ± 0.288
1.092ArgPro: 1.092 ± 0.174
1.268ArgGln: 1.268 ± 0.177
1.479ArgArg: 1.479 ± 0.22
2.43ArgSer: 2.43 ± 0.279
2.36ArgThr: 2.36 ± 0.294
3.276ArgVal: 3.276 ± 0.301
0.528ArgTrp: 0.528 ± 0.148
1.691ArgTyr: 1.691 ± 0.249
0.0ArgXaa: 0.0 ± 0.0
Ser
4.825SerAla: 4.825 ± 0.415
0.106SerCys: 0.106 ± 0.062
6.023SerAsp: 6.023 ± 0.462
4.086SerGlu: 4.086 ± 0.471
2.571SerPhe: 2.571 ± 0.339
5.706SerGly: 5.706 ± 0.627
1.268SerHis: 1.268 ± 0.202
5.283SerIle: 5.283 ± 0.503
6.516SerLys: 6.516 ± 0.618
6.586SerLeu: 6.586 ± 0.582
1.655SerMet: 1.655 ± 0.24
5.741SerAsn: 5.741 ± 0.495
1.972SerPro: 1.972 ± 0.248
2.853SerGln: 2.853 ± 0.316
2.888SerArg: 2.888 ± 0.315
6.551SerSer: 6.551 ± 0.827
4.684SerThr: 4.684 ± 0.61
5.001SerVal: 5.001 ± 0.436
0.669SerTrp: 0.669 ± 0.147
3.557SerTyr: 3.557 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
3.452ThrAla: 3.452 ± 0.422
0.423ThrCys: 0.423 ± 0.12
4.473ThrAsp: 4.473 ± 0.35
2.853ThrGlu: 2.853 ± 0.297
2.642ThrPhe: 2.642 ± 0.3
4.438ThrGly: 4.438 ± 0.551
0.881ThrHis: 0.881 ± 0.174
4.191ThrIle: 4.191 ± 0.461
4.649ThrLys: 4.649 ± 0.34
5.354ThrLeu: 5.354 ± 0.436
1.092ThrMet: 1.092 ± 0.232
4.896ThrAsn: 4.896 ± 0.468
2.43ThrPro: 2.43 ± 0.319
1.902ThrGln: 1.902 ± 0.202
2.078ThrArg: 2.078 ± 0.292
4.508ThrSer: 4.508 ± 0.66
4.262ThrThr: 4.262 ± 0.585
5.318ThrVal: 5.318 ± 0.514
0.634ThrTrp: 0.634 ± 0.169
3.381ThrTyr: 3.381 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
4.121ValAla: 4.121 ± 0.367
0.493ValCys: 0.493 ± 0.158
5.565ValAsp: 5.565 ± 0.454
3.91ValGlu: 3.91 ± 0.508
2.325ValPhe: 2.325 ± 0.274
3.029ValGly: 3.029 ± 0.37
0.845ValHis: 0.845 ± 0.166
4.262ValIle: 4.262 ± 0.396
5.389ValLys: 5.389 ± 0.406
5.072ValLeu: 5.072 ± 0.491
1.127ValMet: 1.127 ± 0.223
4.79ValAsn: 4.79 ± 0.493
2.219ValPro: 2.219 ± 0.285
2.325ValGln: 2.325 ± 0.383
2.148ValArg: 2.148 ± 0.265
5.917ValSer: 5.917 ± 0.526
4.684ValThr: 4.684 ± 0.386
3.628ValVal: 3.628 ± 0.416
0.669ValTrp: 0.669 ± 0.172
3.311ValTyr: 3.311 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
0.493TrpAla: 0.493 ± 0.119
0.07TrpCys: 0.07 ± 0.056
0.528TrpAsp: 0.528 ± 0.127
0.493TrpGlu: 0.493 ± 0.119
0.458TrpPhe: 0.458 ± 0.138
0.845TrpGly: 0.845 ± 0.161
0.282TrpHis: 0.282 ± 0.093
0.669TrpIle: 0.669 ± 0.187
0.81TrpLys: 0.81 ± 0.144
0.951TrpLeu: 0.951 ± 0.211
0.176TrpMet: 0.176 ± 0.084
0.634TrpAsn: 0.634 ± 0.132
0.106TrpPro: 0.106 ± 0.06
0.247TrpGln: 0.247 ± 0.098
0.211TrpArg: 0.211 ± 0.098
0.81TrpSer: 0.81 ± 0.166
0.564TrpThr: 0.564 ± 0.138
0.704TrpVal: 0.704 ± 0.151
0.106TrpTrp: 0.106 ± 0.057
0.775TrpTyr: 0.775 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.24TyrAla: 3.24 ± 0.362
0.528TyrCys: 0.528 ± 0.163
3.346TyrAsp: 3.346 ± 0.41
2.501TyrGlu: 2.501 ± 0.349
1.515TyrPhe: 1.515 ± 0.203
3.346TyrGly: 3.346 ± 0.335
1.233TyrHis: 1.233 ± 0.222
3.487TyrIle: 3.487 ± 0.381
2.818TyrLys: 2.818 ± 0.275
3.839TyrLeu: 3.839 ± 0.374
0.775TyrMet: 0.775 ± 0.15
3.804TyrAsn: 3.804 ± 0.428
1.374TyrPro: 1.374 ± 0.263
1.655TyrGln: 1.655 ± 0.263
2.043TyrArg: 2.043 ± 0.263
3.698TyrSer: 3.698 ± 0.453
3.205TyrThr: 3.205 ± 0.299
3.205TyrVal: 3.205 ± 0.28
0.599TyrTrp: 0.599 ± 0.152
2.712TyrTyr: 2.712 ± 0.296
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (28393 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski