Tortoise microvirus 105
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W817|A0A4P8W817_9VIRU Replication initiation protein OS=Tortoise microvirus 105 OX=2583105 PE=4 SV=1
MM1 pKa = 6.74 KK2 pKa = 10.32 QIVIRR7 pKa = 11.84 LIAPQVPSYY16 pKa = 11.0 DD17 pKa = 3.47 VTVGEE22 pKa = 4.91 IIDD25 pKa = 3.94 GQFAPVNDD33 pKa = 4.02 INQIDD38 pKa = 4.15 PKK40 pKa = 10.21 LANVLIDD47 pKa = 5.57 DD48 pKa = 4.24 VTQSDD53 pKa = 3.75 ILGGSSYY60 pKa = 10.43 IRR62 pKa = 11.84 SKK64 pKa = 11.13 DD65 pKa = 3.47 LSHH68 pKa = 7.46 LLGSLYY74 pKa = 10.52 ASNMISNVTMFPNFTVIDD92 pKa = 4.83 LINYY96 pKa = 8.37 EE97 pKa = 4.34 SEE99 pKa = 4.19 EE100 pKa = 4.23 SS101 pKa = 3.4
Molecular weight: 11.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.165
IPC2_protein 4.05
IPC_protein 3.973
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.342
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.088
Patrickios 3.427
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 4.01
Protein with the highest isoelectric point:
>tr|A0A4P8WAB0|A0A4P8WAB0_9VIRU Uncharacterized protein OS=Tortoise microvirus 105 OX=2583105 PE=4 SV=1
MM1 pKa = 7.47 ACSHH5 pKa = 7.13 PIWIRR10 pKa = 11.84 NRR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 9.64 YY15 pKa = 10.65 DD16 pKa = 3.04 KK17 pKa = 11.0 KK18 pKa = 10.02 NPSRR22 pKa = 11.84 LGSDD26 pKa = 3.14 IQQSALALRR35 pKa = 11.84 PWDD38 pKa = 4.19 VSRR41 pKa = 11.84 QWLMVPCGHH50 pKa = 7.23 CDD52 pKa = 3.18 DD53 pKa = 4.21 CLRR56 pKa = 11.84 RR57 pKa = 11.84 LRR59 pKa = 11.84 NDD61 pKa = 2.4 WFVRR65 pKa = 11.84 LDD67 pKa = 3.87 RR68 pKa = 11.84 EE69 pKa = 4.07 LAHH72 pKa = 6.49 NKK74 pKa = 9.88 SINSQAVFITITISPKK90 pKa = 9.13 YY91 pKa = 8.64 YY92 pKa = 10.39 DD93 pKa = 3.5 RR94 pKa = 11.84 AFSEE98 pKa = 4.41 PAWFIRR104 pKa = 11.84 KK105 pKa = 7.35 WLEE108 pKa = 3.76 RR109 pKa = 11.84 VRR111 pKa = 11.84 HH112 pKa = 4.97 KK113 pKa = 10.74 VGHH116 pKa = 5.22 SFKK119 pKa = 10.69 HH120 pKa = 6.15 AFFQEE125 pKa = 4.74 FGSHH129 pKa = 7.28 PEE131 pKa = 4.05 TGSEE135 pKa = 3.99 PRR137 pKa = 11.84 LHH139 pKa = 6.05 FHH141 pKa = 6.84 GFLFGLDD148 pKa = 3.0 ISYY151 pKa = 11.1 DD152 pKa = 3.33 RR153 pKa = 11.84 LRR155 pKa = 11.84 AVVGDD160 pKa = 4.83 LGFIWVARR168 pKa = 11.84 ATQRR172 pKa = 11.84 RR173 pKa = 11.84 ARR175 pKa = 11.84 YY176 pKa = 5.2 TVKK179 pKa = 10.85 YY180 pKa = 9.67 VVKK183 pKa = 10.49 QINYY187 pKa = 9.97 DD188 pKa = 3.53 GNNPKK193 pKa = 10.01 LRR195 pKa = 11.84 ALLQHH200 pKa = 6.37 RR201 pKa = 11.84 RR202 pKa = 11.84 YY203 pKa = 7.92 TRR205 pKa = 11.84 KK206 pKa = 9.33 FVSAGLGDD214 pKa = 3.79 YY215 pKa = 10.6 LGNKK219 pKa = 7.4 PAPSFHH225 pKa = 6.08 IRR227 pKa = 11.84 SWSYY231 pKa = 10.81 LDD233 pKa = 3.11 TSSRR237 pKa = 11.84 VNYY240 pKa = 10.12 DD241 pKa = 3.17 YY242 pKa = 11.23 AIPRR246 pKa = 11.84 YY247 pKa = 8.56 YY248 pKa = 10.49 NRR250 pKa = 11.84 HH251 pKa = 6.08 LKK253 pKa = 10.63 PEE255 pKa = 4.32 DD256 pKa = 3.36 EE257 pKa = 4.46 LRR259 pKa = 11.84 RR260 pKa = 11.84 SVLAADD266 pKa = 4.33 TYY268 pKa = 11.94 ARR270 pKa = 11.84 FSKK273 pKa = 10.74 SPLVRR278 pKa = 11.84 HH279 pKa = 5.52 IVSRR283 pKa = 11.84 CVNLFIPEE291 pKa = 4.02 ASLSRR296 pKa = 11.84 RR297 pKa = 11.84 ASYY300 pKa = 8.35 TWQLEE305 pKa = 3.93 QMNKK309 pKa = 8.88 FRR311 pKa = 11.84 TAGRR315 pKa = 11.84 PLPAFDD321 pKa = 4.95 PPTFLTRR328 pKa = 11.84 QIVLFWYY335 pKa = 10.21 DD336 pKa = 3.47 NFKK339 pKa = 11.26 LSITT343 pKa = 3.88
Molecular weight: 40.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.692
IPC_protein 10.365
Toseland 10.218
ProMoST 10.028
Dawson 10.452
Bjellqvist 10.189
Wikipedia 10.672
Rodwell 10.643
Grimsley 10.54
Solomon 10.496
Lehninger 10.452
Nozaki 10.233
DTASelect 10.189
Thurlkill 10.292
EMBOSS 10.643
Sillero 10.365
Patrickios 10.072
IPC_peptide 10.482
IPC2_peptide 9.194
IPC2.peptide.svr19 8.557
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1666
68
601
238.0
26.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.983 ± 1.423
0.72 ± 0.383
5.882 ± 0.818
3.842 ± 0.742
4.982 ± 0.814
6.242 ± 0.747
1.861 ± 0.57
5.522 ± 1.168
3.962 ± 1.034
7.623 ± 0.527
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.921 ± 0.447
5.642 ± 0.599
4.742 ± 0.729
4.682 ± 0.775
6.423 ± 1.342
8.764 ± 0.859
6.182 ± 1.155
5.942 ± 0.457
1.861 ± 0.371
5.222 ± 0.967
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here