Alistipes putredinis CAG:67
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1913 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7JRS1|R7JRS1_9BACT Uncharacterized protein OS=Alistipes putredinis CAG:67 OX=1263036 GN=BN752_01193 PE=4 SV=1
MM1 pKa = 7.5 ARR3 pKa = 11.84 NIIDD7 pKa = 5.41 LICNSCSCGKK17 pKa = 10.43 EE18 pKa = 3.76 EE19 pKa = 4.17 AQEE22 pKa = 4.0 YY23 pKa = 10.71 LDD25 pKa = 5.32 DD26 pKa = 5.17 EE27 pKa = 4.38 IRR29 pKa = 11.84 NLQEE33 pKa = 3.7 LQADD37 pKa = 3.52 NDD39 pKa = 3.62 LRR41 pKa = 11.84 GEE43 pKa = 4.17 DD44 pKa = 4.73 FEE46 pKa = 4.85 IACSNLGLDD55 pKa = 4.42 QDD57 pKa = 3.69 WQIYY61 pKa = 8.32 FINRR65 pKa = 11.84 LAGLL69 pKa = 3.89
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.897
IPC_protein 3.808
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|R7JR39|R7JR39_9BACT YCII domain-containing protein OS=Alistipes putredinis CAG:67 OX=1263036 GN=BN752_01447 PE=3 SV=1
MM1 pKa = 7.84 PNGKK5 pKa = 8.55 KK6 pKa = 9.72 HH7 pKa = 6.1 KK8 pKa = 7.52 RR9 pKa = 11.84 HH10 pKa = 5.93 KK11 pKa = 9.95 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.95
IPC_protein 12.442
Toseland 12.647
ProMoST 13.1
Dawson 12.647
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.676
Grimsley 12.676
Solomon 13.115
Lehninger 13.027
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.398
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.949
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1913
0
1913
604354
29
1717
315.9
35.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.626 ± 0.053
1.279 ± 0.022
5.59 ± 0.045
6.787 ± 0.06
4.329 ± 0.041
7.343 ± 0.052
1.814 ± 0.026
6.457 ± 0.051
5.156 ± 0.047
9.232 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.587 ± 0.031
3.839 ± 0.038
4.148 ± 0.034
3.221 ± 0.028
6.318 ± 0.07
5.697 ± 0.048
5.618 ± 0.048
6.973 ± 0.052
1.116 ± 0.024
3.868 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here