Amino acid dipepetide frequency for Citrobacter phage phiCFP-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.946AlaAla: 10.946 ± 1.083
0.788AlaCys: 0.788 ± 0.243
5.604AlaAsp: 5.604 ± 0.614
5.954AlaGlu: 5.954 ± 0.918
3.765AlaPhe: 3.765 ± 0.646
6.918AlaGly: 6.918 ± 0.98
1.664AlaHis: 1.664 ± 0.33
5.692AlaIle: 5.692 ± 0.62
5.692AlaLys: 5.692 ± 0.844
7.618AlaLeu: 7.618 ± 0.816
3.415AlaMet: 3.415 ± 0.567
4.203AlaAsn: 4.203 ± 0.705
2.014AlaPro: 2.014 ± 0.483
3.765AlaGln: 3.765 ± 0.733
5.254AlaArg: 5.254 ± 0.696
4.991AlaSer: 4.991 ± 0.722
3.415AlaThr: 3.415 ± 0.692
6.743AlaVal: 6.743 ± 0.714
1.664AlaTrp: 1.664 ± 0.431
2.715AlaTyr: 2.715 ± 0.479
0.0AlaXaa: 0.0 ± 0.0
Cys
0.525CysAla: 0.525 ± 0.272
0.0CysCys: 0.0 ± 0.0
0.525CysAsp: 0.525 ± 0.214
0.701CysGlu: 0.701 ± 0.333
0.438CysPhe: 0.438 ± 0.251
0.788CysGly: 0.788 ± 0.284
0.35CysHis: 0.35 ± 0.212
0.701CysIle: 0.701 ± 0.288
0.438CysLys: 0.438 ± 0.212
0.701CysLeu: 0.701 ± 0.25
0.088CysMet: 0.088 ± 0.103
0.0CysAsn: 0.0 ± 0.0
0.263CysPro: 0.263 ± 0.17
0.438CysGln: 0.438 ± 0.215
0.525CysArg: 0.525 ± 0.27
0.35CysSer: 0.35 ± 0.199
0.175CysThr: 0.175 ± 0.131
0.175CysVal: 0.175 ± 0.132
0.088CysTrp: 0.088 ± 0.095
0.438CysTyr: 0.438 ± 0.229
0.0CysXaa: 0.0 ± 0.0
Asp
6.217AspAla: 6.217 ± 0.55
0.35AspCys: 0.35 ± 0.173
4.291AspAsp: 4.291 ± 0.527
4.729AspGlu: 4.729 ± 0.798
2.627AspPhe: 2.627 ± 0.538
6.655AspGly: 6.655 ± 0.851
0.963AspHis: 0.963 ± 0.264
3.152AspIle: 3.152 ± 0.486
3.853AspLys: 3.853 ± 0.716
4.116AspLeu: 4.116 ± 0.639
2.364AspMet: 2.364 ± 0.321
2.627AspAsn: 2.627 ± 0.53
2.977AspPro: 2.977 ± 0.444
1.839AspGln: 1.839 ± 0.426
2.977AspArg: 2.977 ± 0.449
3.415AspSer: 3.415 ± 0.522
4.028AspThr: 4.028 ± 0.499
4.203AspVal: 4.203 ± 0.62
0.963AspTrp: 0.963 ± 0.298
1.751AspTyr: 1.751 ± 0.384
0.0AspXaa: 0.0 ± 0.0
Glu
7.18GluAla: 7.18 ± 0.913
0.525GluCys: 0.525 ± 0.207
4.816GluAsp: 4.816 ± 0.778
5.254GluGlu: 5.254 ± 1.184
2.627GluPhe: 2.627 ± 0.427
4.466GluGly: 4.466 ± 0.631
1.926GluHis: 1.926 ± 0.499
3.24GluIle: 3.24 ± 0.405
4.116GluLys: 4.116 ± 0.717
5.954GluLeu: 5.954 ± 0.585
2.89GluMet: 2.89 ± 0.742
2.89GluAsn: 2.89 ± 0.466
1.926GluPro: 1.926 ± 0.36
4.116GluGln: 4.116 ± 0.906
4.028GluArg: 4.028 ± 0.554
4.116GluSer: 4.116 ± 0.608
3.065GluThr: 3.065 ± 0.603
4.553GluVal: 4.553 ± 0.788
1.138GluTrp: 1.138 ± 0.338
3.24GluTyr: 3.24 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.715PheAla: 2.715 ± 0.401
0.263PheCys: 0.263 ± 0.179
3.065PheAsp: 3.065 ± 0.6
2.014PheGlu: 2.014 ± 0.359
1.138PhePhe: 1.138 ± 0.383
3.327PheGly: 3.327 ± 0.565
0.525PheHis: 0.525 ± 0.236
1.926PheIle: 1.926 ± 0.443
2.89PheLys: 2.89 ± 0.499
2.89PheLeu: 2.89 ± 0.501
1.138PheMet: 1.138 ± 0.286
2.189PheAsn: 2.189 ± 0.462
1.576PhePro: 1.576 ± 0.397
1.401PheGln: 1.401 ± 0.316
1.751PheArg: 1.751 ± 0.324
2.102PheSer: 2.102 ± 0.543
2.802PheThr: 2.802 ± 0.653
2.102PheVal: 2.102 ± 0.407
0.088PheTrp: 0.088 ± 0.077
1.138PheTyr: 1.138 ± 0.351
0.0PheXaa: 0.0 ± 0.0
Gly
6.305GlyAla: 6.305 ± 0.815
0.613GlyCys: 0.613 ± 0.326
5.079GlyAsp: 5.079 ± 0.791
5.254GlyGlu: 5.254 ± 0.755
3.24GlyPhe: 3.24 ± 0.443
6.48GlyGly: 6.48 ± 0.904
1.226GlyHis: 1.226 ± 0.312
4.816GlyIle: 4.816 ± 0.644
5.867GlyLys: 5.867 ± 0.758
7.005GlyLeu: 7.005 ± 0.805
2.277GlyMet: 2.277 ± 0.379
3.765GlyAsn: 3.765 ± 0.57
0.701GlyPro: 0.701 ± 0.231
2.452GlyGln: 2.452 ± 0.445
4.466GlyArg: 4.466 ± 0.514
4.553GlySer: 4.553 ± 0.684
3.065GlyThr: 3.065 ± 0.584
4.991GlyVal: 4.991 ± 0.601
1.489GlyTrp: 1.489 ± 0.447
2.189GlyTyr: 2.189 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
1.576HisAla: 1.576 ± 0.398
0.175HisCys: 0.175 ± 0.117
0.788HisAsp: 0.788 ± 0.211
1.401HisGlu: 1.401 ± 0.428
0.876HisPhe: 0.876 ± 0.219
1.401HisGly: 1.401 ± 0.374
0.438HisHis: 0.438 ± 0.19
1.576HisIle: 1.576 ± 0.353
1.138HisLys: 1.138 ± 0.303
2.014HisLeu: 2.014 ± 0.408
0.438HisMet: 0.438 ± 0.175
1.051HisAsn: 1.051 ± 0.303
0.788HisPro: 0.788 ± 0.32
0.263HisGln: 0.263 ± 0.291
0.35HisArg: 0.35 ± 0.151
1.313HisSer: 1.313 ± 0.391
0.876HisThr: 0.876 ± 0.238
1.401HisVal: 1.401 ± 0.342
0.438HisTrp: 0.438 ± 0.176
0.963HisTyr: 0.963 ± 0.291
0.0HisXaa: 0.0 ± 0.0
Ile
3.59IleAla: 3.59 ± 0.55
0.35IleCys: 0.35 ± 0.161
3.94IleAsp: 3.94 ± 0.603
4.116IleGlu: 4.116 ± 0.708
1.489IlePhe: 1.489 ± 0.391
3.59IleGly: 3.59 ± 0.55
1.489IleHis: 1.489 ± 0.329
3.152IleIle: 3.152 ± 0.38
4.116IleLys: 4.116 ± 0.665
3.678IleLeu: 3.678 ± 0.557
0.963IleMet: 0.963 ± 0.27
2.715IleAsn: 2.715 ± 0.84
2.627IlePro: 2.627 ± 0.448
1.926IleGln: 1.926 ± 0.465
2.89IleArg: 2.89 ± 0.527
3.065IleSer: 3.065 ± 0.481
2.452IleThr: 2.452 ± 0.457
2.977IleVal: 2.977 ± 0.403
0.613IleTrp: 0.613 ± 0.2
2.102IleTyr: 2.102 ± 0.392
0.0IleXaa: 0.0 ± 0.0
Lys
7.356LysAla: 7.356 ± 0.966
0.613LysCys: 0.613 ± 0.287
4.028LysAsp: 4.028 ± 0.577
5.079LysGlu: 5.079 ± 0.658
2.539LysPhe: 2.539 ± 0.439
4.904LysGly: 4.904 ± 0.689
1.576LysHis: 1.576 ± 0.415
1.576LysIle: 1.576 ± 0.283
4.641LysLys: 4.641 ± 1.149
5.342LysLeu: 5.342 ± 0.793
1.664LysMet: 1.664 ± 0.376
2.014LysAsn: 2.014 ± 0.3
3.065LysPro: 3.065 ± 0.659
2.715LysGln: 2.715 ± 0.557
3.765LysArg: 3.765 ± 0.617
3.503LysSer: 3.503 ± 0.701
2.977LysThr: 2.977 ± 0.375
5.779LysVal: 5.779 ± 0.541
0.525LysTrp: 0.525 ± 0.22
2.014LysTyr: 2.014 ± 0.323
0.0LysXaa: 0.0 ± 0.0
Leu
7.356LeuAla: 7.356 ± 1.475
0.175LeuCys: 0.175 ± 0.168
4.466LeuAsp: 4.466 ± 0.446
6.48LeuGlu: 6.48 ± 0.992
2.802LeuPhe: 2.802 ± 0.485
4.641LeuGly: 4.641 ± 0.486
1.313LeuHis: 1.313 ± 0.359
4.028LeuIle: 4.028 ± 0.666
6.655LeuLys: 6.655 ± 0.87
5.342LeuLeu: 5.342 ± 0.694
2.364LeuMet: 2.364 ± 0.404
4.116LeuAsn: 4.116 ± 0.653
3.678LeuPro: 3.678 ± 0.555
3.853LeuGln: 3.853 ± 0.633
4.904LeuArg: 4.904 ± 0.653
4.729LeuSer: 4.729 ± 0.641
4.904LeuThr: 4.904 ± 0.679
4.729LeuVal: 4.729 ± 0.601
1.313LeuTrp: 1.313 ± 0.47
2.452LeuTyr: 2.452 ± 0.435
0.0LeuXaa: 0.0 ± 0.0
Met
3.327MetAla: 3.327 ± 0.525
0.175MetCys: 0.175 ± 0.122
1.489MetAsp: 1.489 ± 0.428
1.664MetGlu: 1.664 ± 0.494
0.963MetPhe: 0.963 ± 0.315
1.926MetGly: 1.926 ± 0.465
0.701MetHis: 0.701 ± 0.239
1.489MetIle: 1.489 ± 0.301
0.701MetLys: 0.701 ± 0.236
3.853MetLeu: 3.853 ± 0.627
0.701MetMet: 0.701 ± 0.262
1.489MetAsn: 1.489 ± 0.249
1.664MetPro: 1.664 ± 0.326
1.576MetGln: 1.576 ± 0.443
1.576MetArg: 1.576 ± 0.386
1.489MetSer: 1.489 ± 0.334
2.014MetThr: 2.014 ± 0.387
2.102MetVal: 2.102 ± 0.394
0.175MetTrp: 0.175 ± 0.127
0.525MetTyr: 0.525 ± 0.217
0.0MetXaa: 0.0 ± 0.0
Asn
3.94AsnAla: 3.94 ± 0.411
0.613AsnCys: 0.613 ± 0.286
2.715AsnAsp: 2.715 ± 0.446
3.415AsnGlu: 3.415 ± 0.496
1.489AsnPhe: 1.489 ± 0.431
3.415AsnGly: 3.415 ± 0.467
0.525AsnHis: 0.525 ± 0.224
3.59AsnIle: 3.59 ± 0.861
2.539AsnLys: 2.539 ± 0.431
3.065AsnLeu: 3.065 ± 0.51
1.051AsnMet: 1.051 ± 0.286
2.977AsnAsn: 2.977 ± 0.685
2.452AsnPro: 2.452 ± 0.614
1.926AsnGln: 1.926 ± 0.383
2.977AsnArg: 2.977 ± 0.811
2.977AsnSer: 2.977 ± 0.691
1.664AsnThr: 1.664 ± 0.312
2.364AsnVal: 2.364 ± 0.592
0.701AsnTrp: 0.701 ± 0.226
2.452AsnTyr: 2.452 ± 0.55
0.0AsnXaa: 0.0 ± 0.0
Pro
2.89ProAla: 2.89 ± 0.392
0.438ProCys: 0.438 ± 0.19
2.715ProAsp: 2.715 ± 0.406
3.327ProGlu: 3.327 ± 0.581
1.489ProPhe: 1.489 ± 0.271
1.839ProGly: 1.839 ± 0.381
0.613ProHis: 0.613 ± 0.192
1.313ProIle: 1.313 ± 0.318
2.539ProLys: 2.539 ± 0.577
2.539ProLeu: 2.539 ± 0.613
1.138ProMet: 1.138 ± 0.304
2.627ProAsn: 2.627 ± 0.641
1.138ProPro: 1.138 ± 0.362
1.138ProGln: 1.138 ± 0.376
2.014ProArg: 2.014 ± 0.433
1.751ProSer: 1.751 ± 0.363
2.014ProThr: 2.014 ± 0.508
2.452ProVal: 2.452 ± 0.497
0.701ProTrp: 0.701 ± 0.164
1.226ProTyr: 1.226 ± 0.389
0.0ProXaa: 0.0 ± 0.0
Gln
4.466GlnAla: 4.466 ± 0.914
0.088GlnCys: 0.088 ± 0.077
2.014GlnAsp: 2.014 ± 0.441
3.678GlnGlu: 3.678 ± 0.409
1.576GlnPhe: 1.576 ± 0.329
2.89GlnGly: 2.89 ± 0.41
0.613GlnHis: 0.613 ± 0.221
2.014GlnIle: 2.014 ± 0.483
2.364GlnLys: 2.364 ± 0.397
3.853GlnLeu: 3.853 ± 0.501
0.788GlnMet: 0.788 ± 0.283
1.839GlnAsn: 1.839 ± 0.447
1.226GlnPro: 1.226 ± 0.389
1.751GlnGln: 1.751 ± 0.404
2.014GlnArg: 2.014 ± 0.376
2.277GlnSer: 2.277 ± 0.489
1.926GlnThr: 1.926 ± 0.485
2.802GlnVal: 2.802 ± 0.509
0.788GlnTrp: 0.788 ± 0.218
1.226GlnTyr: 1.226 ± 0.394
0.0GlnXaa: 0.0 ± 0.0
Arg
5.779ArgAla: 5.779 ± 0.726
0.876ArgCys: 0.876 ± 0.274
3.59ArgAsp: 3.59 ± 0.703
3.678ArgGlu: 3.678 ± 0.648
2.715ArgPhe: 2.715 ± 0.473
3.503ArgGly: 3.503 ± 0.563
1.138ArgHis: 1.138 ± 0.33
2.539ArgIle: 2.539 ± 0.556
3.503ArgLys: 3.503 ± 0.581
5.342ArgLeu: 5.342 ± 0.636
1.839ArgMet: 1.839 ± 0.397
2.802ArgAsn: 2.802 ± 0.592
1.401ArgPro: 1.401 ± 0.349
2.102ArgGln: 2.102 ± 0.408
2.977ArgArg: 2.977 ± 0.397
4.553ArgSer: 4.553 ± 0.576
2.977ArgThr: 2.977 ± 0.44
3.327ArgVal: 3.327 ± 0.538
0.613ArgTrp: 0.613 ± 0.217
0.963ArgTyr: 0.963 ± 0.183
0.0ArgXaa: 0.0 ± 0.0
Ser
5.692SerAla: 5.692 ± 1.079
0.35SerCys: 0.35 ± 0.169
5.429SerAsp: 5.429 ± 0.739
3.765SerGlu: 3.765 ± 0.583
2.715SerPhe: 2.715 ± 0.456
5.342SerGly: 5.342 ± 0.747
1.138SerHis: 1.138 ± 0.27
2.627SerIle: 2.627 ± 0.542
3.152SerLys: 3.152 ± 0.549
3.59SerLeu: 3.59 ± 0.589
1.576SerMet: 1.576 ± 0.427
2.014SerAsn: 2.014 ± 0.455
1.839SerPro: 1.839 ± 0.482
1.839SerGln: 1.839 ± 0.368
3.503SerArg: 3.503 ± 0.491
3.678SerSer: 3.678 ± 0.683
3.59SerThr: 3.59 ± 0.629
3.59SerVal: 3.59 ± 0.497
0.613SerTrp: 0.613 ± 0.143
2.539SerTyr: 2.539 ± 0.534
0.0SerXaa: 0.0 ± 0.0
Thr
3.853ThrAla: 3.853 ± 0.646
0.613ThrCys: 0.613 ± 0.319
2.452ThrAsp: 2.452 ± 0.408
3.415ThrGlu: 3.415 ± 0.624
1.664ThrPhe: 1.664 ± 0.371
5.254ThrGly: 5.254 ± 0.755
1.226ThrHis: 1.226 ± 0.361
3.503ThrIle: 3.503 ± 0.443
4.028ThrLys: 4.028 ± 0.45
4.291ThrLeu: 4.291 ± 0.612
1.401ThrMet: 1.401 ± 0.35
2.014ThrAsn: 2.014 ± 0.355
2.802ThrPro: 2.802 ± 0.373
2.102ThrGln: 2.102 ± 0.419
2.627ThrArg: 2.627 ± 0.474
3.415ThrSer: 3.415 ± 0.53
3.503ThrThr: 3.503 ± 0.61
3.415ThrVal: 3.415 ± 0.532
0.175ThrTrp: 0.175 ± 0.107
1.926ThrTyr: 1.926 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
5.342ValAla: 5.342 ± 0.725
0.263ValCys: 0.263 ± 0.136
3.327ValAsp: 3.327 ± 0.523
5.254ValGlu: 5.254 ± 0.625
1.751ValPhe: 1.751 ± 0.478
5.342ValGly: 5.342 ± 0.751
0.876ValHis: 0.876 ± 0.236
2.802ValIle: 2.802 ± 0.53
4.378ValLys: 4.378 ± 0.658
4.816ValLeu: 4.816 ± 0.589
1.839ValMet: 1.839 ± 0.424
3.065ValAsn: 3.065 ± 0.513
2.627ValPro: 2.627 ± 0.459
2.715ValGln: 2.715 ± 0.486
4.553ValArg: 4.553 ± 0.582
3.94ValSer: 3.94 ± 0.484
4.904ValThr: 4.904 ± 0.546
5.166ValVal: 5.166 ± 0.698
0.876ValTrp: 0.876 ± 0.315
2.102ValTyr: 2.102 ± 0.425
0.0ValXaa: 0.0 ± 0.0
Trp
0.876TrpAla: 0.876 ± 0.206
0.175TrpCys: 0.175 ± 0.142
0.788TrpAsp: 0.788 ± 0.265
0.701TrpGlu: 0.701 ± 0.271
0.525TrpPhe: 0.525 ± 0.207
0.788TrpGly: 0.788 ± 0.27
0.263TrpHis: 0.263 ± 0.16
0.35TrpIle: 0.35 ± 0.254
1.138TrpLys: 1.138 ± 0.321
1.664TrpLeu: 1.664 ± 0.424
0.438TrpMet: 0.438 ± 0.188
0.963TrpAsn: 0.963 ± 0.302
0.263TrpPro: 0.263 ± 0.14
0.613TrpGln: 0.613 ± 0.229
0.788TrpArg: 0.788 ± 0.209
0.613TrpSer: 0.613 ± 0.319
1.051TrpThr: 1.051 ± 0.306
1.226TrpVal: 1.226 ± 0.426
0.35TrpTrp: 0.35 ± 0.156
0.175TrpTyr: 0.175 ± 0.108
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.89TyrAla: 2.89 ± 0.578
0.263TyrCys: 0.263 ± 0.154
2.977TyrAsp: 2.977 ± 0.718
2.189TyrGlu: 2.189 ± 0.428
0.613TyrPhe: 0.613 ± 0.3
2.364TyrGly: 2.364 ± 0.403
0.701TyrHis: 0.701 ± 0.212
1.751TyrIle: 1.751 ± 0.509
1.926TyrLys: 1.926 ± 0.294
2.627TyrLeu: 2.627 ± 0.452
1.138TyrMet: 1.138 ± 0.287
1.489TyrAsn: 1.489 ± 0.315
0.876TyrPro: 0.876 ± 0.235
1.751TyrGln: 1.751 ± 0.413
2.277TyrArg: 2.277 ± 0.434
1.751TyrSer: 1.751 ± 0.463
2.277TyrThr: 2.277 ± 0.572
1.839TyrVal: 1.839 ± 0.393
0.438TyrTrp: 0.438 ± 0.19
0.525TyrTyr: 0.525 ± 0.239
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (11421 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski