Aeromonas phage 3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A219Y9U0|A0A219Y9U0_9CAUD Uncharacterized protein OS=Aeromonas phage 3 OX=1932897 PE=4 SV=1
MM1 pKa = 7.58AKK3 pKa = 9.48WFAYY7 pKa = 10.53SPDD10 pKa = 3.38GGMSFHH16 pKa = 7.2DD17 pKa = 3.76TPEE20 pKa = 3.92EE21 pKa = 3.77AKK23 pKa = 10.42AWAQRR28 pKa = 11.84EE29 pKa = 3.84IDD31 pKa = 3.83DD32 pKa = 5.15CRR34 pKa = 11.84DD35 pKa = 3.23AMDD38 pKa = 4.49DD39 pKa = 4.18LGWEE43 pKa = 4.2EE44 pKa = 4.99DD45 pKa = 4.27LVSGICWGQVKK56 pKa = 9.38EE57 pKa = 4.07QAKK60 pKa = 9.97EE61 pKa = 4.04VIPLDD66 pKa = 4.12AYY68 pKa = 10.59GSDD71 pKa = 4.07YY72 pKa = 11.05EE73 pKa = 5.17LEE75 pKa = 4.15AAA77 pKa = 5.07

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219Y921|A0A219Y921_9CAUD Uncharacterized protein OS=Aeromonas phage 3 OX=1932897 PE=4 SV=1
MM1 pKa = 7.47PVKK4 pKa = 10.25IPVIATSIATGEE16 pKa = 4.27VVRR19 pKa = 11.84FEE21 pKa = 4.73SVTSAARR28 pKa = 11.84VGGFDD33 pKa = 3.35RR34 pKa = 11.84TCVQACIHH42 pKa = 5.88GQRR45 pKa = 11.84KK46 pKa = 7.16HH47 pKa = 6.39HH48 pKa = 6.65NGFIFTTPATLRR60 pKa = 11.84QTSADD65 pKa = 3.73TLIHH69 pKa = 6.99KK70 pKa = 9.35IANYY74 pKa = 10.03RR75 pKa = 11.84NQGYY79 pKa = 6.91TNKK82 pKa = 8.99ATAAIMGLSASTVRR96 pKa = 11.84NRR98 pKa = 11.84VPMARR103 pKa = 11.84HH104 pKa = 5.26MKK106 pKa = 9.99LLKK109 pKa = 10.74DD110 pKa = 3.78GLL112 pKa = 4.01

Molecular weight: 12.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

14040

40

868

169.2

18.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.335 ± 0.4

1.368 ± 0.129

5.299 ± 0.199

6.489 ± 0.329

2.97 ± 0.146

7.657 ± 0.377

2.393 ± 0.215

4.9 ± 0.187

6.026 ± 0.256

8.725 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.419 ± 0.14

3.447 ± 0.216

4.238 ± 0.259

3.896 ± 0.218

6.054 ± 0.291

5.869 ± 0.251

6.011 ± 0.182

7.073 ± 0.246

1.574 ± 0.108

2.258 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski