Tomato leaf curl Moheli virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7DXK4|A7DXK4_9GEMI C4 protein OS=Tomato leaf curl Moheli virus OX=439425 GN=c4 PE=3 SV=1
MM1 pKa = 7.62GNLICMPLFNSRR13 pKa = 11.84EE14 pKa = 3.96NSRR17 pKa = 11.84APTIASSIAYY27 pKa = 5.69TQAVAPVSTPTYY39 pKa = 10.26KK40 pKa = 10.5EE41 pKa = 3.86LSPALTSSPIWTRR54 pKa = 11.84TEE56 pKa = 4.01TPLSGEE62 pKa = 4.58SFRR65 pKa = 11.84SMDD68 pKa = 4.68DD69 pKa = 3.41LQEE72 pKa = 4.33GDD74 pKa = 3.81NNQPMTLTPKK84 pKa = 10.8LLTQAVSQRR93 pKa = 11.84LLEE96 pKa = 4.29SLGSS100 pKa = 3.61

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7DXK2|A7DXK2_9GEMI Replication enhancer OS=Tomato leaf curl Moheli virus OX=439425 GN=c3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.29SPFTSRR30 pKa = 11.84AVVPIVPGTSRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.79RR48 pKa = 11.84PMYY51 pKa = 9.54RR52 pKa = 11.84KK53 pKa = 8.92PRR55 pKa = 11.84MYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 10.57RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 6.99EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.05DD83 pKa = 3.72VKK85 pKa = 9.5HH86 pKa = 5.48TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.36VTRR98 pKa = 11.84GPGITHH104 pKa = 6.76RR105 pKa = 11.84VGKK108 pKa = 9.5RR109 pKa = 11.84FCVKK113 pKa = 10.3SIYY116 pKa = 10.1ILGKK120 pKa = 9.09IWMDD124 pKa = 3.64EE125 pKa = 4.09NIKK128 pKa = 10.06KK129 pKa = 9.97QNHH132 pKa = 4.95TNQVMFFLVRR142 pKa = 11.84DD143 pKa = 3.77RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.07GAAPMDD153 pKa = 4.26FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.81NDD173 pKa = 2.84LRR175 pKa = 11.84DD176 pKa = 3.47RR177 pKa = 11.84YY178 pKa = 9.28QVLRR182 pKa = 11.84KK183 pKa = 8.77FHH185 pKa = 5.93ATVVGGPSGMKK196 pKa = 9.47EE197 pKa = 3.54QALVKK202 pKa = 10.34RR203 pKa = 11.84FFKK206 pKa = 10.71INSHH210 pKa = 4.14VTYY213 pKa = 10.54NHH215 pKa = 5.79QEE217 pKa = 3.49AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.63FYY253 pKa = 11.33DD254 pKa = 3.78SIGNN258 pKa = 3.69

Molecular weight: 29.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

745

100

258

149.0

17.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.966 ± 0.657

2.282 ± 0.624

4.966 ± 0.574

4.295 ± 0.532

4.295 ± 0.757

4.564 ± 0.563

4.161 ± 1.005

6.309 ± 0.922

4.966 ± 0.761

6.846 ± 1.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.492

4.564 ± 1.152

6.309 ± 0.741

5.235 ± 0.842

8.188 ± 0.814

7.651 ± 1.6

5.772 ± 0.895

6.711 ± 1.18

1.074 ± 0.145

4.027 ± 0.561
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski