Ruminococcaceae bacterium AM28-23LB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2802 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417HLU2|A0A417HLU2_9FIRM DUF134 domain-containing protein OS=Ruminococcaceae bacterium AM28-23LB OX=2292269 GN=DW741_04745 PE=3 SV=1
MM1 pKa = 7.58TKK3 pKa = 10.54DD4 pKa = 3.29QLLSLWNADD13 pKa = 2.72NWEE16 pKa = 4.06VMSCGVYY23 pKa = 8.33FTAHH27 pKa = 6.82RR28 pKa = 11.84ADD30 pKa = 4.19KK31 pKa = 10.18EE32 pKa = 3.98LHH34 pKa = 6.43INCNDD39 pKa = 3.3YY40 pKa = 10.93TEE42 pKa = 5.23AEE44 pKa = 4.21ILAMPFWEE52 pKa = 4.77RR53 pKa = 11.84LAQEE57 pKa = 4.66LDD59 pKa = 3.38EE60 pKa = 5.5LDD62 pKa = 3.93RR63 pKa = 11.84QAHH66 pKa = 6.52EE67 pKa = 4.16ILQKK71 pKa = 10.58EE72 pKa = 4.37FPDD75 pKa = 5.56DD76 pKa = 3.82EE77 pKa = 6.01DD78 pKa = 5.44IPDD81 pKa = 4.64LPLTDD86 pKa = 3.23ITIDD90 pKa = 3.23KK91 pKa = 9.47SGCYY95 pKa = 9.0GTFSLCYY102 pKa = 9.4DD103 pKa = 4.01TGDD106 pKa = 3.66SPAGEE111 pKa = 4.66LYY113 pKa = 11.09LNVSFDD119 pKa = 3.85EE120 pKa = 4.32QFVPSPKK127 pKa = 10.07VGYY130 pKa = 8.25DD131 pKa = 3.22TFF133 pKa = 5.17

Molecular weight: 15.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A417HNM2|A0A417HNM2_9FIRM D-2-hydroxyacid dehydrogenase OS=Ruminococcaceae bacterium AM28-23LB OX=2292269 GN=DW741_03400 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 10.23RR3 pKa = 11.84CIIRR7 pKa = 11.84LALVFLVLFLISLFFRR23 pKa = 11.84FTAGGGLWEE32 pKa = 4.32TLCITFAVTAYY43 pKa = 9.68HH44 pKa = 6.11FWMRR48 pKa = 11.84LGVGQLLNHH57 pKa = 6.34TLQNNVDD64 pKa = 4.21FTKK67 pKa = 10.06PWFRR71 pKa = 11.84PRR73 pKa = 11.84RR74 pKa = 11.84FEE76 pKa = 3.88PFLYY80 pKa = 9.22RR81 pKa = 11.84TIRR84 pKa = 11.84VKK86 pKa = 10.1KK87 pKa = 7.97WKK89 pKa = 10.15KK90 pKa = 10.1HH91 pKa = 3.84MPTYY95 pKa = 10.41DD96 pKa = 2.98AAYY99 pKa = 10.27FDD101 pKa = 4.6IRR103 pKa = 11.84THH105 pKa = 6.86SITEE109 pKa = 4.13IIKK112 pKa = 10.34AGCQAEE118 pKa = 4.37IVHH121 pKa = 6.62EE122 pKa = 4.31IIIVLSFLPVGLAFWFRR139 pKa = 11.84PAGVFLATSFLSALVDD155 pKa = 3.57SLFVIMQRR163 pKa = 11.84YY164 pKa = 7.25NRR166 pKa = 11.84PRR168 pKa = 11.84LLKK171 pKa = 10.53LVSRR175 pKa = 5.0

Molecular weight: 20.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2802

0

2802

841265

37

2434

300.2

33.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.533 ± 0.042

1.692 ± 0.019

5.206 ± 0.037

7.339 ± 0.059

3.926 ± 0.033

7.869 ± 0.055

1.865 ± 0.024

5.56 ± 0.039

5.384 ± 0.041

10.129 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.022

3.289 ± 0.032

4.26 ± 0.031

4.32 ± 0.041

5.483 ± 0.046

5.708 ± 0.043

5.257 ± 0.05

6.78 ± 0.04

1.057 ± 0.019

3.429 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski