Magnaporthe oryzae ourmia-like virus
Average proteome isoelectric point is 9.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M4HCZ9|A0A1M4HCZ9_9VIRU RNA dependent RNA polymerase OS=Magnaporthe oryzae ourmia-like virus OX=1858671 GN=RdRp PE=4 SV=1
MM1 pKa = 7.63 SYY3 pKa = 10.66 SEE5 pKa = 4.55 SDD7 pKa = 3.07 GSAACNSAGRR17 pKa = 11.84 VAVPSLQMRR26 pKa = 11.84 NLVSFSGLLSEE37 pKa = 4.7 TFRR40 pKa = 11.84 VDD42 pKa = 3.3 LRR44 pKa = 11.84 VPEE47 pKa = 4.13 TLGDD51 pKa = 3.63 STRR54 pKa = 11.84 EE55 pKa = 3.83 LKK57 pKa = 10.42 EE58 pKa = 3.48 FCGRR62 pKa = 11.84 LLEE65 pKa = 4.54 PSGSHH70 pKa = 5.82 PWAHH74 pKa = 7.2 ILRR77 pKa = 11.84 PLPAVDD83 pKa = 3.85 RR84 pKa = 11.84 LSIAGSLFSFSKK96 pKa = 9.66 TLPSSDD102 pKa = 4.62 PDD104 pKa = 3.19 CDD106 pKa = 3.25 AYY108 pKa = 10.58 VRR110 pKa = 11.84 HH111 pKa = 5.9 MSVPGVKK118 pKa = 9.45 TPDD121 pKa = 3.0 GYY123 pKa = 11.57 LRR125 pKa = 11.84 FVRR128 pKa = 11.84 KK129 pKa = 10.05 LIMRR133 pKa = 11.84 EE134 pKa = 4.23 FPVGWDD140 pKa = 3.12 KK141 pKa = 11.32 GWRR144 pKa = 11.84 STYY147 pKa = 10.79 LGTTPTSSSALGSSKK162 pKa = 10.9 GKK164 pKa = 10.65 GGARR168 pKa = 11.84 YY169 pKa = 8.06 QLRR172 pKa = 11.84 GSRR175 pKa = 11.84 QWFLKK180 pKa = 10.21 SCMGLEE186 pKa = 4.24 RR187 pKa = 11.84 EE188 pKa = 4.46 LLLDD192 pKa = 3.57 PVRR195 pKa = 11.84 KK196 pKa = 10.09 VGIARR201 pKa = 11.84 CDD203 pKa = 3.36 GKK205 pKa = 10.63 SRR207 pKa = 11.84 RR208 pKa = 11.84 VTQSPAEE215 pKa = 4.21 SVVLSPLHH223 pKa = 4.95 TLIYY227 pKa = 10.4 NHH229 pKa = 7.33 LSRR232 pKa = 11.84 KK233 pKa = 9.34 DD234 pKa = 3.04 WLLRR238 pKa = 11.84 GEE240 pKa = 4.08 ATPDD244 pKa = 3.05 KK245 pKa = 10.76 FADD248 pKa = 4.28 FCQKK252 pKa = 10.56 DD253 pKa = 3.67 GEE255 pKa = 4.69 VFVSGDD261 pKa = 3.54 YY262 pKa = 10.98 EE263 pKa = 4.32 SASDD267 pKa = 3.79 NLSIEE272 pKa = 4.03 AAEE275 pKa = 4.4 TVLSAIFAKK284 pKa = 10.38 ARR286 pKa = 11.84 YY287 pKa = 8.32 IPQAIRR293 pKa = 11.84 RR294 pKa = 11.84 VAMDD298 pKa = 3.31 SLRR301 pKa = 11.84 CVLVSEE307 pKa = 4.58 GAVGIQARR315 pKa = 11.84 GQLMGNFLCFPLLCLQNYY333 pKa = 9.16 AAFRR337 pKa = 11.84 YY338 pKa = 9.25 LAGNYY343 pKa = 7.31 PVRR346 pKa = 11.84 INGDD350 pKa = 3.8 DD351 pKa = 3.22 IVFRR355 pKa = 11.84 APEE358 pKa = 3.86 HH359 pKa = 6.22 VRR361 pKa = 11.84 ARR363 pKa = 11.84 WAAGVQALGLTLSVGKK379 pKa = 8.33 TFVHH383 pKa = 6.56 KK384 pKa = 10.64 RR385 pKa = 11.84 FFSLNSTYY393 pKa = 10.23 FRR395 pKa = 11.84 ARR397 pKa = 11.84 TKK399 pKa = 10.87 NGVKK403 pKa = 10.02 GVPIVRR409 pKa = 11.84 ATLFYY414 pKa = 10.77 KK415 pKa = 10.67 GVEE418 pKa = 4.3 SADD421 pKa = 3.86 SIVGRR426 pKa = 11.84 FSSVGSFFPVATSSMLRR443 pKa = 11.84 VRR445 pKa = 11.84 ILQAMQKK452 pKa = 9.96 EE453 pKa = 4.48 IRR455 pKa = 11.84 CTQRR459 pKa = 11.84 SVRR462 pKa = 11.84 RR463 pKa = 11.84 GLGLNATPAEE473 pKa = 4.18 LARR476 pKa = 11.84 SGLLVRR482 pKa = 11.84 EE483 pKa = 4.28 KK484 pKa = 10.51 FYY486 pKa = 11.54 SNLPRR491 pKa = 11.84 EE492 pKa = 4.27 SAVPSKK498 pKa = 10.7 LAVSGALPPGWVKK511 pKa = 10.04 VRR513 pKa = 11.84 PLWTGMTLVEE523 pKa = 4.47 DD524 pKa = 4.22 PEE526 pKa = 4.48 FKK528 pKa = 10.62 KK529 pKa = 10.83 EE530 pKa = 4.25 LVQHH534 pKa = 6.45 CWNSVVSRR542 pKa = 11.84 VEE544 pKa = 3.95 TQDD547 pKa = 2.76 YY548 pKa = 8.89 WNVVRR553 pKa = 11.84 SGGCPYY559 pKa = 10.76 RR560 pKa = 11.84 STSGQAYY567 pKa = 9.07 RR568 pKa = 11.84 RR569 pKa = 11.84 KK570 pKa = 10.4 AKK572 pKa = 10.07 LLKK575 pKa = 10.54 LSVNATRR582 pKa = 11.84 RR583 pKa = 11.84 YY584 pKa = 10.23 LEE586 pKa = 4.16 GRR588 pKa = 11.84 LGAVPPRR595 pKa = 11.84 KK596 pKa = 9.7 KK597 pKa = 10.73 GVWQRR602 pKa = 11.84 ADD604 pKa = 3.14 RR605 pKa = 4.36
Molecular weight: 67.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.399
IPC_protein 9.648
Toseland 10.16
ProMoST 9.897
Dawson 10.35
Bjellqvist 10.043
Wikipedia 10.526
Rodwell 10.643
Grimsley 10.409
Solomon 10.379
Lehninger 10.35
Nozaki 10.204
DTASelect 10.028
Thurlkill 10.204
EMBOSS 10.57
Sillero 10.277
Patrickios 10.189
IPC_peptide 10.379
IPC2_peptide 9.004
IPC2.peptide.svr19 8.393
Protein with the highest isoelectric point:
>tr|A0A1M4HCZ9|A0A1M4HCZ9_9VIRU RNA dependent RNA polymerase OS=Magnaporthe oryzae ourmia-like virus OX=1858671 GN=RdRp PE=4 SV=1
MM1 pKa = 7.63 SYY3 pKa = 10.66 SEE5 pKa = 4.55 SDD7 pKa = 3.07 GSAACNSAGRR17 pKa = 11.84 VAVPSLQMRR26 pKa = 11.84 NLVSFSGLLSEE37 pKa = 4.7 TFRR40 pKa = 11.84 VDD42 pKa = 3.3 LRR44 pKa = 11.84 VPEE47 pKa = 4.13 TLGDD51 pKa = 3.63 STRR54 pKa = 11.84 EE55 pKa = 3.83 LKK57 pKa = 10.42 EE58 pKa = 3.48 FCGRR62 pKa = 11.84 LLEE65 pKa = 4.54 PSGSHH70 pKa = 5.82 PWAHH74 pKa = 7.2 ILRR77 pKa = 11.84 PLPAVDD83 pKa = 3.85 RR84 pKa = 11.84 LSIAGSLFSFSKK96 pKa = 9.66 TLPSSDD102 pKa = 4.62 PDD104 pKa = 3.19 CDD106 pKa = 3.25 AYY108 pKa = 10.58 VRR110 pKa = 11.84 HH111 pKa = 5.9 MSVPGVKK118 pKa = 9.45 TPDD121 pKa = 3.0 GYY123 pKa = 11.57 LRR125 pKa = 11.84 FVRR128 pKa = 11.84 KK129 pKa = 10.05 LIMRR133 pKa = 11.84 EE134 pKa = 4.23 FPVGWDD140 pKa = 3.12 KK141 pKa = 11.32 GWRR144 pKa = 11.84 STYY147 pKa = 10.79 LGTTPTSSSALGSSKK162 pKa = 10.9 GKK164 pKa = 10.65 GGARR168 pKa = 11.84 YY169 pKa = 8.06 QLRR172 pKa = 11.84 GSRR175 pKa = 11.84 QWFLKK180 pKa = 10.21 SCMGLEE186 pKa = 4.24 RR187 pKa = 11.84 EE188 pKa = 4.46 LLLDD192 pKa = 3.57 PVRR195 pKa = 11.84 KK196 pKa = 10.09 VGIARR201 pKa = 11.84 CDD203 pKa = 3.36 GKK205 pKa = 10.63 SRR207 pKa = 11.84 RR208 pKa = 11.84 VTQSPAEE215 pKa = 4.21 SVVLSPLHH223 pKa = 4.95 TLIYY227 pKa = 10.4 NHH229 pKa = 7.33 LSRR232 pKa = 11.84 KK233 pKa = 9.34 DD234 pKa = 3.04 WLLRR238 pKa = 11.84 GEE240 pKa = 4.08 ATPDD244 pKa = 3.05 KK245 pKa = 10.76 FADD248 pKa = 4.28 FCQKK252 pKa = 10.56 DD253 pKa = 3.67 GEE255 pKa = 4.69 VFVSGDD261 pKa = 3.54 YY262 pKa = 10.98 EE263 pKa = 4.32 SASDD267 pKa = 3.79 NLSIEE272 pKa = 4.03 AAEE275 pKa = 4.4 TVLSAIFAKK284 pKa = 10.38 ARR286 pKa = 11.84 YY287 pKa = 8.32 IPQAIRR293 pKa = 11.84 RR294 pKa = 11.84 VAMDD298 pKa = 3.31 SLRR301 pKa = 11.84 CVLVSEE307 pKa = 4.58 GAVGIQARR315 pKa = 11.84 GQLMGNFLCFPLLCLQNYY333 pKa = 9.16 AAFRR337 pKa = 11.84 YY338 pKa = 9.25 LAGNYY343 pKa = 7.31 PVRR346 pKa = 11.84 INGDD350 pKa = 3.8 DD351 pKa = 3.22 IVFRR355 pKa = 11.84 APEE358 pKa = 3.86 HH359 pKa = 6.22 VRR361 pKa = 11.84 ARR363 pKa = 11.84 WAAGVQALGLTLSVGKK379 pKa = 8.33 TFVHH383 pKa = 6.56 KK384 pKa = 10.64 RR385 pKa = 11.84 FFSLNSTYY393 pKa = 10.23 FRR395 pKa = 11.84 ARR397 pKa = 11.84 TKK399 pKa = 10.87 NGVKK403 pKa = 10.02 GVPIVRR409 pKa = 11.84 ATLFYY414 pKa = 10.77 KK415 pKa = 10.67 GVEE418 pKa = 4.3 SADD421 pKa = 3.86 SIVGRR426 pKa = 11.84 FSSVGSFFPVATSSMLRR443 pKa = 11.84 VRR445 pKa = 11.84 ILQAMQKK452 pKa = 9.96 EE453 pKa = 4.48 IRR455 pKa = 11.84 CTQRR459 pKa = 11.84 SVRR462 pKa = 11.84 RR463 pKa = 11.84 GLGLNATPAEE473 pKa = 4.18 LARR476 pKa = 11.84 SGLLVRR482 pKa = 11.84 EE483 pKa = 4.28 KK484 pKa = 10.51 FYY486 pKa = 11.54 SNLPRR491 pKa = 11.84 EE492 pKa = 4.27 SAVPSKK498 pKa = 10.7 LAVSGALPPGWVKK511 pKa = 10.04 VRR513 pKa = 11.84 PLWTGMTLVEE523 pKa = 4.47 DD524 pKa = 4.22 PEE526 pKa = 4.48 FKK528 pKa = 10.62 KK529 pKa = 10.83 EE530 pKa = 4.25 LVQHH534 pKa = 6.45 CWNSVVSRR542 pKa = 11.84 VEE544 pKa = 3.95 TQDD547 pKa = 2.76 YY548 pKa = 8.89 WNVVRR553 pKa = 11.84 SGGCPYY559 pKa = 10.76 RR560 pKa = 11.84 STSGQAYY567 pKa = 9.07 RR568 pKa = 11.84 RR569 pKa = 11.84 KK570 pKa = 10.4 AKK572 pKa = 10.07 LLKK575 pKa = 10.54 LSVNATRR582 pKa = 11.84 RR583 pKa = 11.84 YY584 pKa = 10.23 LEE586 pKa = 4.16 GRR588 pKa = 11.84 LGAVPPRR595 pKa = 11.84 KK596 pKa = 9.7 KK597 pKa = 10.73 GVWQRR602 pKa = 11.84 ADD604 pKa = 3.14 RR605 pKa = 4.36
Molecular weight: 67.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.399
IPC_protein 9.648
Toseland 10.16
ProMoST 9.897
Dawson 10.35
Bjellqvist 10.043
Wikipedia 10.526
Rodwell 10.643
Grimsley 10.409
Solomon 10.379
Lehninger 10.35
Nozaki 10.204
DTASelect 10.028
Thurlkill 10.204
EMBOSS 10.57
Sillero 10.277
Patrickios 10.189
IPC_peptide 10.379
IPC2_peptide 9.004
IPC2.peptide.svr19 8.393
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
605
605
605
605.0
67.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.934 ± 0.0
1.983 ± 0.0
3.967 ± 0.0
4.463 ± 0.0
4.298 ± 0.0
8.099 ± 0.0
1.322 ± 0.0
2.645 ± 0.0
4.959 ± 0.0
10.413 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.653 ± 0.0
2.479 ± 0.0
5.289 ± 0.0
2.81 ± 0.0
9.752 ± 0.0
10.083 ± 0.0
4.298 ± 0.0
8.76 ± 0.0
1.818 ± 0.0
2.975 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here